Final Days: Chinese Scientists Have Solved the DP Culturing Problem! (2014)

[Arashi]

Hell, they even did it themselves "The cells were able to produce human epidermis and hair follicles, which Xu says were “nearly identical to the epithelial stem cells directly isolated from a hair follicle—biochemically, in gene expression, as well as functionally". So they already made a follicle in the lab. So again, that remark has been taken out of context. I think he's talking about making EVERYTHING out of iPS cells (epethelial + DP cells) AND most probably about the day a therapy based on that can hit the market. That's nice, but there's just no need (from the market, us baldies) if DP cells can be cultured.

[Arashi]

The 10 years estimate come from this article http://www.upenn.edu/pennnews/curren...inst-hair-loss

I'm pretty sure that comment is taken out of context by the journalist: "He says the ultimate goal is to be able to make a hair follicle in a lab". People already made hair follicles in the lab ! Like Tsuji lab did. Maybe the researcher said that it will be 10 years before it's on the market. Or, maybe he meant that he wants to make a follice totally derived from iPSC cells (so the DP cells too). That maybe complex (and also unnecessary since they can now culture the cells, so no need to induce them from iPSC, it's just an alternative route). But the way it is stated there, just makes NO sense. Again, follicles have already been made in the lab.

[534623]

You give me their resources, funding and past knowledge... I would LAUGH at the idea of taking 10 years to do put the theory into practice.

I totally agree with you. Especially with such a very simple approach ...

They just took existing cells and hair grew.

[Jonathan]

Have anyone emailed the Chinese researchers and asked for more details regarding their time estimates, clinical trials etc? I don't have enough knowledge to know what to ask them (if you post suggestions on what to ask them here, I will be happy to send the email myself)

[Jonathan]

Who did actually say 10 years? I'd rather wait for an official statemant than believing in random multiples of 2 and 5.

The 10 years estimate come from this article http://www.upenn.edu/pennnews/curren...inst-hair-loss

[ytterligare]

Lets put our hope on that they say 10 years instead of 1 year, just to cheat their competitors

Who did actually say 10 years? I'd rather wait for an official statemant than believing in random multiples of 2 and 5.

[Jonathan]

You give me their resources, funding and past knowledge... I would LAUGH at the idea of taking 10 years to do put the theory into practice.

Let's not pretend like 10 years is acceptable. That's friggin' pitiful.

Whats your theory behind this? Don't you think the researching companies and the investors behind them understand the economic value of being the first company that presents a cure for baldness?

If they really could do it in shorter time if they put more effort into it, then I agree with you that it is pitiful, but I don't think that's the case. Why would they say no to wealth and fame? It doesn't make sense.

Lets put our hope on that they say 10 years instead of 1 year, just to cheat their competitors

[nameless]

Hi Guys,

The scientists @ Nanfang Hospital of Southern Medical University in China just published this article. They confirmed that the expression of several genes and proteins associated with hair follicle inductivity of DP cells, such as NCAM, Versican and α-SMA were maintained using this 3D Matrigel Culturing Method.

THREE DIFFERENT TEAMS FROM ALL OVER THE WORLD HAVE MANAGED TO CRACK THIS ISSUE IN THE LAST 3 MONTHS Jahoda/Christiano, Taiwan Uni & Now the Chinese. We are so close Here's the abstract:

Controllable production of transplantable adult human high-passage dermal papilla spheroids using 3D Matrigel culture

We have succeeded in culturing human dermal papilla (DP) cells spheroids and developed a three-dimensional Matrigel (basement membrane matrix) culture technique that can enhance and restores DP cells unique characteristics in vitro.

When 10000 DP cells were cultured on the 96 well plates pre-coated with Matrigel for 5 days, both passage 2 and passage 8 DP cells formed spheroidal microtissues with a diameter of 150-250 μm in an aggregative and proliferative manner. We transferred and re-cultured these DP spheroids onto commercial plates. Cells within DP spheres could disaggregate and migrate out, which was similar to primary DP. Moreover, we examined the expression of several genes and proteins associated with hair follicle inductivity of DP cells, such as NCAM, Versican and α-SMA, and confirmed that their expression level was elevated in the spheres compared with the dissociated DP cells. To examine hair-inducing ability of DP spheres, hair germinal matrix cells and DP spheres were mixed and cultured on Matrigel. Unlike the dissociated DP cells and hair germinal matrix cells co-cultured in two dimensions, hair germinal matrix cells can differentiate into hair-like fibers under the induction of the DP spheres made from the high passage cells (passage 8) in vitro.

We are the first to show that passage 3 human hair germinal matrix cells differentiate into hair-like fiber in the presence of human DP spheroids.

These results suggest that three-dimensional Matrigel culture technique is an ideal culture model for forming DP spheroids and that sphere formation partially models the intact DP, resulting in hair induction, even by high passage DP cells.

http://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2013.0547

__________________________________________________ _______________

My brothers are getting the article for me Once I read it I'll update you guys

Desmond, the Chinese talks about partial preservation of trichogenicity/induction, not 100% preservation of it. Jahoda already accomplished partial preservation last year so the Chinese have not accomplished a big advance unless they have preserved a lot more trichogenicity than Jahoda did.

[nameless]

You give me their resources, funding and past knowledge... I would LAUGH at the idea of taking 10 years to do put the theory into practice.

Let's not pretend like 10 years is acceptable. That's friggin' pitiful.

They were probably talking about 10 years to get the treatment to market. Which is a point I've been making the past few days.

I think with the focus now on trichogenicity, and with the researchers going at the problem full-tilt-boogie, they will probably solve the problem within 2 years. It may just be a year or even months, but then it will take another 6 - 8 years to get it into the mainstream marketplace. I've said this repeatedly for a few days now.

[greatjob!]

You give me their resources, funding and past knowledge... I would LAUGH at the idea of taking 10 years to do put the theory into practice.

Let's not pretend like 10 years is acceptable. That's friggin' pitiful.

LOL!! Yes give me all the knowledge, all the money, and all the resources and I will fix everything....troll level= 1000

[hellouser]

Can either of you do it any faster?

You give me their resources, funding and past knowledge... I would LAUGH at the idea of taking 10 years to do put the theory into practice.

Let's not pretend like 10 years is acceptable. That's friggin' pitiful.

[yeahyeahyeah]

If you truly stopped checking this place, you would not be posting now! There's the door...

I check from time to time see if there is anything new.

[greatjob!]

You stop posting on this web site and live your life.

I have stopped checking this place because it's the same rehashed material being posted.

I'm glad you quit posting here concidering you have a perfect head of hair.

[Mike K]

You stop posting on this web site and live your life.

I have stopped checking this place because it's the same rehashed material being posted.

If you truly stopped checking this place, you would not be posting now! There's the door...

[yeahyeahyeah]

I seriously want to hurt someone when I read this kind of bullsh*t. Why TEN years? What's the effing hold up? Did they really crack the final piece of the puzzle, call it a day and say 'we'll come back in ten years' ? What the hell are they going to do for the next TEN YEARS? Thats 3650 DAYS... of doing what?

You stop posting on this web site and live your life.

I have stopped checking this place because it's the same rehashed material being posted.