Updated Research and Knowledge - Cutting Edge

[Keeper]

L-ascorbic acid 2-phosphate is possible too buy around 40 dollars

[Forestry]

other dkk1 inhibitors

Review of dkk1:

http://www.jidonline.org/article/S0022-202X%2815%2933732-5/pdf

Dihydrotestosterone-Inducible Dickkopf 1 from Balding Dermal Papilla Cells Causes Apoptosis in Follicular Keratinocytes

http://www.freepatentsonline.com/y2010/0040619.html

"Dickkopf-1 (Dkk-1) is a member of the dickkopf family of proteins that have been shown to be negative regulators of the canonical Wnt-signaling pathway, which has a central role in bone development and formation (see, for example, Glinka et al., Nature 391:357-62 (1998); Fedi et al., J Biol Chem 274(27):19465-72 (1999); Zorn, Curr Biol 11:R592-95 (2001); and Krupnik et al., Gene 238: 301-13 (1999)). Dkk-1 inhibits Wnt signaling through its interaction with the Wnt co-receptors LRP5 or LRP6 and the kremen proteins (see, for example, Bafico et al., Nature Cell Biol 3:683 (2001); Mao et al., Nature 411(17):321 (2001); Mao et al., Nature 417:664 (2002); and Semënov et al., Curr Biol 11:951-61 (2001). By binding LRP5 (LRP6) and kremen proteins, Dkk-1 prevents LRP5 or LRP6 from associating with members of the Wnt pathway and thus prevents Wnt-mediated signal transduction, which in turn results in the inhibition of bone formation.

LRP5 is a key protein in regulating bone mass (see, for example, Gong et al., Cell 107:513-23 (2001); Patel, N Eng J Med 346(20):1572 (2002)). An autosomal recessive disorder characterized by low bone mass (osteoporosis-pseudoglioma syndrome, or “OPPG”) has been identified as being caused by loss-of-function mutations in LRP5 (Gong et al., 2001). In addition, gain-of-function mutations in LRP5 have been shown to result in autosomal dominant high bone mass in humans (Little et al., Am J Human Genetics. 70(1):11-19, 2002). The same mutations in LRP5 that result in high bone mass can interfere with the ability of Dkk-1 to inhibit LRP5 signaling (see, for example, Boyden et al., N Eng J Med. 346(20):1513-1521, 2002). Thus, Dkk-1 is appropriately characterized as being a negative regulator of bone deposition.

In view of the involvement of Dkk-1 in the regulation of bone formation and its role in various other diseases that are associated with bone loss (e.g., cancer and diabetes), there is a need for improved anti-Dkk-1 antibodies for therapeutic use and for other purposes."

http://www.ncbi.nlm.nih.gov/gene/22943

other dkk1 blockers:

Horny Goat Weed: "Flavonoids of Herba Epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/beta-catenin signaling pathway." http://www.ncbi.nlm.nih.gov/pubmed/19703516

L-ascorbic acid 2-phosphate represses the dihydrotestosterone-induced dickkopf-1 expression in human balding dermal papilla cells. http://www.ncbi.nlm.nih.gov/pubmed/20701628

Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosterone-induced dickkopf-1 expression in human hair dermal papilla cells. http://www.ncbi.nlm.nih.gov/pubmed/21034532

[Seuxin]

Can really be improve with an AA ( Duta, Fin, RU, CB)

[bananana]

Here is the full resolution BEFORE/AFTER pic.

When you zoom in you can see some progress, but it's too early to say for sure, in my opinion.

http://postimg.org/image/o3vhp8lij/full/

Lemme know what do you think.

[Seuxin]

joshuk, you can whitout problems ;

@Chemical : These studies about rice bran supercritical CO2 extract are very awesome !

It could be a great and cheap addition to our regimen !

Here is a link..if we used at 1%...it could last very long time

http://www.aromantic.co.uk/home/products/botanical-co2-extracts/organic-rice-bran-oil-co2-extract.aspx

Need investigations !

[joshuk]

chemical do you think adding OL to lipogaine would work, only reason im asking is becasue its a diffrent type of vehicle

[Forestry]

rice bran second study

Too bad, the second link is "16-week double-blind randomized controlled single-center trial to evaluate the safety and efficacy of dermal rice bran supercritical CO2 extract (RB-SCE) in the treatment of androgenic alopecia."

I read the second study, which confirms RB-SCE as inhibiting the 5-AR enzyme in androgen-responsive organs (the skin organ in this case). the results were "similar to slightly lower than those reported for 2% minoxidil after 48 weeks or 1% pro-cyanidin B-2 after 16 weeks."

I was also interested in this study in that they published their stack:

INCI................................... % (as w/w)
----------------------------- -----------
Water (aqua), demineralized . as 100%
Glycerin................................ 1.0
Hydroxyethyl cellulose............ 0.5
Tetrasodium EDTA................. 0.03
C12–14 Pareth-12................. 2.0
Hyaluronic acid...................... 0.5
Alcohol................................ 15.0
RB-SCE................................ 0.5
PEG-40 castor oil................... 2.0
Triethanolamine..................... 0.3

INCI = International Nomenclature of Cosmetic Ingredients;
RB-SCE = rice bran supercritical CO2 extract.

I did not look at all of these, but i did look at Hyaluronic Acid.

It appears (total guess on my part) that HA may be added due to it's value in cell proliferation and migration, and possible reduction of normally occuring local HA synthesis due to the lowering of TGF-Beta1 by RB-SCE (as noted in the first rice bran study in SriHanuman's post).

http://www.jbc.org/content/290/52/30910.long

This study does mention high molecular weight HA (might be the type used in the RB-SCE study (?)).

"The turnover of HA in most tissues is extraordinarily rapid; the half-life of HA in the skin, which contains about half of all HA in the body, is 1–1.5 days. Thus, the tight control of HA synthesis and degradation is necessary for this turnover and seems to finely balance the amounts of high molecular mass HA (1,000–10,000 kDa) within tissues. On the other hand, an imbalance of synthesis and degradation causes the accumulation of HA with different molecular weights, which is commonly observed in diseases such as arthritis and cancers."

"TGF-β1 conferred the most potent effect on production of high molecular weight HA by up-regulation of HAS expression and down-regulation of HYBID (HYaluronan-Binding protein Involvedin hyaluronan Depolymerization), aka KIAA1199, expression via distinct signaling pathways."

[Forestry]

rice bran second study

Too bad, the second link is "16-week double-blind randomized controlled single-center trial to evaluate the safety and efficacy of dermal rice bran supercritical CO2 extract (RB-SCE) in the treatment of androgenic alopecia."

I read the second study, which confirms RB-SCE as inhibiting the 5-AR enzyme in androgen-responsive organs (the skin organ in this case). the results were "similar to slightly lower than those reported for 2% minoxidil after 48 weeks or 1% pro
-cyanidin B-2 after 16 weeks."

I was also interested in this study in that they published their stack:1

INCI % (as w/w)
----------------------------
Water (aqua), demineralized as 100%
Glycerin 1.0
Hydroxyethyl cellulose 0.5
Tetrasodium EDTA 0.03
C12–14 Pareth-12 2.0
Hyaluronic acid 0.5
Alcohol 15.0
RB-SCE 0.5
PEG-40 castor oil 2.0
Triethanolamine 0.3

INCI = International Nomenclature of Cosmetic Ingredients;
RB-SCE = rice bran supercritical CO2 extract.

[Bobbylobbyhobbythrobby]

Supplemental hair growth?

Has anyone tried this method (below), or does anyone think that these supplements would help?

http://forum.bodybuilding.com/showthread.php?t=160444941

[Bobbylobbyhobbythrobby]

Has anyone tried this method (below), or does anyone think that these supplements would help?

http://forum.bodybuilding.com/showthread.php?t=160444941

[SriHanuman]

3.- betacatenin can increase the oxidation of body, increase the wrinkles in the front or in the eyes, produce bones calcification? because I have my skin in nice conditions and I don¡t want destroy it

http://www.ncbi.nlm.nih.gov/pubmed/21707762

[pepmorg79]

Hi Chemical,

I am a fan of your studies, I am fighting against hairloss since 6 years at least and I have tried a lot of treatments: FIN, MINOX, RU, KETO, CB, LATANOPROST, SETIPIPRANT, and someone more that now I don't remember it.

Last mont I have started to read you and follow your instructions and my hair are better, I have a nice regrowth that I hope continue next months. My actual treatment is:

- CB 1% (70% ethano 30% propilenglicol) at morning
- OL 1mg/ml (70% ethanol 30 %distilled water) at midday
- 1,15 mg of FIN at midday
- MINOX with OL (minox 5%, OL 1mg/ml (60mg of OL in bottle of 60ml of Minox) at night.

I think the regeneration hair is for the increase of betacatenin which produced Oleuropein, but I have some questions about OLEUROPEIN in my head wich last days I am a little worried, and I am very happy if you can answer me:

1.- betacatenin can increase the risk about the proliferation cancer cells? because I have read something in internet and I am quite worried about it.

2.- betacatenin influence in the sleeping time? because last weeks I sleep worse and a little of insomnia

3.- betacatenin can increase the oxidation of body, increase the wrinkles in the front or in the eyes, produce bones calcification? because I have my skin in nice conditions and I don¡t want destroy it

4.- other question about it is if the antioxidants can reduce the quantity of betacatenin? because I read that some antioxidants like magnesium or vit.d can reduce betacatenin

5.- if are true, what are the antioxidants who affect betacatenin in major percentage? do you know about vit c or vit e effects?
Because the objective global is can regrowth hair without generate radical free or maintaining the skin in good conditions.

6.- the last question is about diet: what can I do with our diet relationed with betacatenin? If I eat a lot of verdures (brocoli, salmon, fishes, calabacin, etc..) which have antioxidant propierties are we reducing the results of OL? Or it is independent and doesn't have relationed?
Because I consume some brocoli for example, the antioxidant propierties, furthermore inhibitor aromatasa action could reduce the benefits of OL?

Sorry for a lot of questions, but I admire your studies about regrwoth hair, you have helped to start regrowth my hair and I am happy for this, now I do this questions because I am apasionate like you about find solutions of hair and I hope this solutions don't damage other things.
However, I am quite worried with the relation about betacatenin and cancer and the oxidation of body and damages skin.

I hope your answer when you can,

[ryan82]

WNT agonist is very important.

TheKaneShop is selling a WNT agonist named "6BIo", as 99$ for 500mg. Nevertheless i don't know the amount to use per day. I think it's a little amount because WNT agonist at high dose can be dangerous.

It can be a good addition...

In addition, Follistatin is VERY IMPORTANT for hair, it's used in the famous Histogen ( HSC) protocol ( KGF, VEGF, and Follistatin), nevertheless AFAIK, there is different type of follistatin....Maybe it could be good to find a simple an cheap way to increase production of follistatin.

-> Increasing VEGF is simple ( With hypoxia like Stemoxydine, or with Minoxidil )
-> Increasing KGF is simple ( With Minoxidil, or buying directky KGF for cheap on the skinactive website )

So we need to find a way to increase Follistatin !

And we have to find the amount to use for 6-BIO.

Bio-6 is a better VPA. Why is it dangerous with high dose? Do you have experience with bio-6?

[SriHanuman]

I've only ready the first link SriHanuman , that is a good read and shows RB-SCE a decent alternative to minoxidil.

Too bad, the second link is "16-week double-blind randomized controlled single-center trial to evaluate the safety and efficacy of dermal rice bran supercritical CO2 extract (RB-SCE) in the treatment of androgenic alopecia."

[burtandernie]

One issue I always have is some things that are natural SOUND good, but do they actually work as the small studies might seem to indicate? Tons of natural products have many of these ingredients like revivogen listed below comes to mind. It never really had much success from user stories back then, and I tried it as well back when it was the latest fad. Why would it not work if it was formulated with many of these natural ingredients your mentioning? Right off the bat it has linolenic acid, beta-sis, saw palmetto among many others.

revivogen
SD Alcohol 40 (Denat. Alcohol), Purified Water (Aqua), Linolenic Acid (Gamma & Alpha), Linoleic Acid, Oleic Acid, Saw Palmetto Extract (Serenoa Serrulata), Ethyl Laurate, Beta-Sitosterol, Caffeine, Octyl (Ethylhexyl) Gallate, Dodecyl Gallate, Pyridoxal 5-Phosphate, Tocopherol (Vitamin E), Procyanidin B2 (Vitis Vinifera Extract), Azelaic Acid , Zinc Sulfate, Menthol, EDTA, Perfume (Parfum).