Updated Research and Knowledge - Cutting Edge

[lucky1987]

got the olive, egcg, gla and rosemary today.
only need to order the alcohol, but the ones being linked to are out.
i wonder if this will be good as wel:

http://www.drank.nl/winkel/pure-alcohol/

its 96% pure alcohol, if i understand it correctly this should work, if anyone can verify this.

also i want to make progression pictures, but i am a diffuse thinner, tried to take pictures, but you just dont see it on in the pictures.
if anyone has some advice on this.

[ryan82]

Somebody already results or it that too early to ask?

[potato1987]

Chemical where are you mate?

[Sogeking]

I got mine in 70% Eth / 20 % PG and it dissolved well enough. Same goes for apple olyphenols, ECGC and rosemary...

[FeelsBad]

Has anyone actually obtained some 20% oleuropein / olive leaf extract and tried dissolving it in a vehicle? It could be one of those things that just floats around in chunks, never dissolving. The oleuropein paper looked pretty good though. Too bad it wasn't a human study, but the mouse data, plus the human DP cell culture data showed a lot of good gene expression going in the desired direction.

I bought some olive leaf extract that was already extracted in a water/glycerin base. It mixes easily with ethanol. Not sure if this is more or less effective though

[InBeforeTheCure]

Has anyone actually obtained some 20% oleuropein / olive leaf extract and tried dissolving it in a vehicle? It could be one of those things that just floats around in chunks, never dissolving. The oleuropein paper looked pretty good though. Too bad it wasn't a human study, but the mouse data, plus the human DP cell culture data showed a lot of good gene expression going in the desired direction.

In a 50% ethanol/30% polysorbate 80/20% dimethyl isosorbide vehicle, it dissolves easily at .75%, the concentration he was using. It won't dissolve at 5% say in that vehicle, but if you first mix it around in a bit of DMSO and then add the other stuff, it works.

[bej]

Has anyone actually obtained some 20% oleuropein / olive leaf extract and tried dissolving it in a vehicle? It could be one of those things that just floats around in chunks, never dissolving. The oleuropein paper looked pretty good though. Too bad it wasn't a human study, but the mouse data, plus the human DP cell culture data showed a lot of good gene expression going in the desired direction.

[bej]

oils

deleted double post

[bej]

Might be on to something there http://www.ncbi.nlm.nih.gov/pubmed/26848219 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737836/. Can buy here: http://www.bulknutrients.com.au/prod...c-acid-40.html

The GLA and LA may have other functions, like inhibiting 5AR, but those activities are probably weak, and trumped by including something like RU in the regimen.

One caveat to all of these oils is whether they will dissolve in a vehicle. In my own experiments at home, I haven't found any oils that mix into a vehicle. One possible exception to that is Castor oil, which I read is miscible with ethanol. I don't have the patience to put several different things on my head every day.

[tiktok]

There was discussion of using gamma linolenic acid and also linoleic acid (both omega-6) topically to help hair. I guess the premise is that they are precursors to eventually be turned into, among other things, PGE2.

I was looking at the pathway diagrams, and arachidonic acid is like 3 or 4 steps closer to PGE2 than those other fatty acids. Wouldn't arachidonic be superior to GLA and LA? Arachidonic acid should be easy to obtain, there are supplements for it.

Might be on to something there http://www.ncbi.nlm.nih.gov/pubmed/26848219 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737836/. Can buy here: http://www.bulknutrients.com.au/prod...c-acid-40.html

[bej]

I was reading through this thread, sifting through the ideas...

There was discussion of using gamma linolenic acid and also linoleic acid (both omega-6) topically to help hair. I guess the premise is that they are precursors to eventually be turned into, among other things, PGE2.

I was looking at the pathway diagrams, and arachidonic acid is like 3 or 4 steps closer to PGE2 than those other fatty acids. Wouldn't arachidonic be superior to GLA and LA? Arachidonic acid should be easy to obtain, there are supplements for it.

[musky]

Yo McChemical dog you have misinterpreted this study

http://www.ncbi.nlm.nih.gov/pubmed/1632630

5 alpha-Dihydrotestosterone, the principal androgen mediating prostate growth and function in the rat, is formed from testosterone by steroid 5 alpha-reductase. The inactivation of 5 alpha-dihydrotestosterone involves reversible reduction to 5 alpha-androstane-3 beta,17 beta-diol by 3 beta-hydroxysteroid oxidoreductase followed by 6 alpha-, 7 alpha-, or 7 beta-hydroxylation. 5 alpha-Androstane-3 beta,17 beta-diol hydroxylation represents the ultimate inactivation step of dihydrotestosterone in rat prostate and is apparently catalyzed by a single, high-affinity (Km approximately 0.5 microM) microsomal cytochrome P450 enzyme. The present studies were designed to determine if 5 alpha-androstane-3 beta,17 beta-diol hydroxylation by rat prostate microsomes is inhibited by agents that are known inhibitors of androgen-metabolizing enzymes. Inhibitors of steroid 5 alpha-reductase (4-azasteroid analogs; 10 microM) or inhibitors of 3 beta-hydroxysteroid oxidoreductase (trilostane, azastene, and cyanoketone; 10 microM) had no appreciable effect on the 6 alpha-, 7 alpha-, or 7 beta-hydroxylation of 5 alpha-androstane-3 beta,17 beta-diol (10 microM) by rat prostate microsomes. Imidazole-type antimycotic drugs (ketoconazole, clotrimazole, and miconazole; 0.1-10 microM) all markedly inhibited 5 alpha-androstane-3 beta,17 beta-diol hydroxylation in a concentration-dependent manner, whereas triazole-type antimycotic drugs (fluconazole and itraconazole; 0.1-10 microM) had no inhibitory effect. The rank order of inhibitory potency of the imidazole-type antimycotic drugs was miconazole greater than clotrimazole greater than ketoconazole. In the case of clotrimazole, the inhibition was shown to be competitive in nature, with a Ki of 0.03 microM. The imidazole-type antimycotic drugs inhibited all three pathways of 5 alpha-androstane-3 beta,17 beta-diol hydroxylation to the same extent, which provides further evidence that, in rat prostate microsomes, a single cytochrome P450 enzyme catalyzes the 6 alpha-, 7 alpha-, and 7 beta-hydroxylation of 5 alpha-androstane-3 beta,17 beta-diol. These studies demonstrate that certain imidazole-type compounds are potent, competitive inhibitors of 5 alpha-androstane-3 beta,17 beta-diol hydroxylation by rat prostate microsomes, which is consistent with the effect of these antimycotic drugs on cytochrome P450 enzymes involved in the metabolism of other androgens and steroids.

Ketocanzole and miconazole inhibited the HYDROXYLATION of beta-diol, this means less beta-diol will be converted into it's hydroxy metabolites, which means if you use Keto there will be MORE beta-diol lying around. Food for thought dog.

[Seuxin]

Thanks Noisette !

Sadlly, it's only on mices...
Formononetin appear to be expansive...

Maybe have to find a natural component who contain it (red clover plants powder ?)

http://www.starwest-botanicals.com/category/red-clover-blossoms/

[noisette]

Topical Treatment of Hair Loss with Formononetin by Modulating Apoptosis.

Kim MH1,*Choi YY1,*Lee JE1,*Kim K2,*Yang WM1.

Author information

1College of Korean Medicine and Institute of Korean Medicine, Kyung Hee University, Seoul, Korea.2Department of Ophthalmology, Otorhinolaryngology and Dermatology of Korean Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.

Abstract

Formononetin is one of the main components of red clover plants and its role on hair regrowth against hair loss has not been established yet. In the present study, we assessed the potential effects of formononetin on alopecia, along with impaired hair cycles by induction of apoptosis-regression.Depilated C57BL/6 mice were used for monitoring the hair cycles. Formononetin (1 and 100 µM) was topically treated to the dorsal skin for 14 days. Topical formononetin treatment induced miniaturized hair follicles to recover to normal sizes. Tapering hair shaft began to grow newly, emerging from the hair follicles by formononetin. In addition, formononetin inhibited the activation of caspase-8 and decreased the procaspase-9 expression. As a result of formononetin treatment, anti-apoptotic Bcl-2 was up-regulated, whereas pro-apoptotic Bax and p53 were down-regulated, resulting in a decrease of caspase-3 activation. Formononetin showed the obvious inhibition of apoptosis under terminal deoxynucleotidyl transferase dUTP nick end labeling staining thereafter.Taken together, our findings demonstrate that formononetin exerted the hair regrowth effect on hair loss, in which the underlying mechanisms were associated with Fas/Fas L-induced caspase activation, thus inhibiting apoptosis.

Georg Thieme Verlag KG Stuttgart · New York

[joshuk]

will be starting again next week with an eth/PG mix of OLE/EGCG/APPLE POLY

also recently bought 10g of CB-03-01 which i will be using again as i stopped all hairloss treatments for 2 weeks as i was in hospital. cant really take stuff like that in their haha.