50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

[534623]

I'm sorry, but how can you have higher than 100% regen in the donor?

Am I missing something?

Yes, you missed something:
Dr. Nigam had big problems at school with percentage calculations.

[534623]

IM is Totally wrong, if you compare with my donor regen which is much higher than 100%, the true invivo stemcell follilce multiplication.

erm, excuse me, but what exactly can be "higher than 100%"??
Let me guess:
You extract 10 grafts and 15 grafts will regrow in the donor area - am I right?
Or you extract 10 single follicles and 17 single follicles will regrow in the donor area - am I right?

I mean, THAT would be "much higher than 100%" regrowth. lol

So can you prove (scientifically or anecdotally or photoshopically) where YOU got "much higher than 100%" regrowth?

[gc83uk]

IM is Totally wrong, if you compare with my donor regen which is much higher than 100%, the true invivo stemcell follilce multiplication.
I should also have less regen if im is to be believed, as my patients will also have follicles in anagen and telogen,
That's why i say, if you don't share and pronounce your invention as secret, you are definitely not confident of your own technique, and can be exposed...,but still will be.
I would like to quote the word's of DR WESLEY..when you find something exciting ..you can't resist sharing.He also said the invention was nothing but common sense,and he wondered why nobody found till now ..this was regarding his trial on scarlesss procedure.
Arashi it is because many a times they extract complete follicles for implantation at recipient as seen in petri dish as shown by ironman that anyone can easily see dermal papilla in the extracted grafts in ironmans petri dish(again im force's me speak)

I'm sorry, but how can you have higher than 100% regen in the donor?

Am I missing something?

[drnigams]

IM is Totally wrong, if you compare with my donor regen which is much higher than 100%, the true invivo stemcell follilce multiplication.
I should also have less regen if im is to be believed, as my patients will also have follicles in anagen and telogen,
That's why i say, if you don't share and pronounce your invention as secret, you are definitely not confident of your own technique, and can be exposed...,but still will be.
I would like to quote the word's of DR WESLEY..when you find something exciting ..you can't resist sharing.He also said the invention was nothing but common sense,and he wondered why nobody found till now ..this was regarding his trial on scarlesss procedure.
Arashi it is because many a times they extract complete follicles for implantation at recipient as seen in petri dish as shown by ironman that anyone can easily see dermal papilla in the extracted grafts in ironmans petri dish(again im force's me speak)

Oh, the answer to this question is rocket sience for Dr. Nigam ...

The 10-20% "failure rate" with Gho's HST technique reflects just the percentage of follicles in TELOGEN versus ANAGEN.

[gc83uk]

Oh, the answer to this question is rocket science for Dr. Nigam ...

The 10-20% "failure rate" with Gho's HST technique reflects just the percentage of follicles in TELOGEN versus ANAGEN in every human scalp.

So it has nothing to do with any "technical failure" per se.

Dr Nigam, how do you avoid this very problem to get 100% regrowth?

Or do you also agree that because of Telogen, it can cause 10% or 20% of grafts not to regrow?

[534623]

But in your opinion, what exactly causes that 20% of donor in the Gho procedure doesn't regrow ?

Oh, the answer to this question is rocket science for Dr. Nigam ...

The 10-20% "failure rate" with Gho's HST technique reflects just the percentage of follicles in TELOGEN versus ANAGEN in every human scalp.

So it has nothing to do with any "technical failure" per se.

[drnigams]

We do not split grafts.
When a girl plucks her eyebrow (mostly a single follicle),
A part of follicle comes out with breakage or bisection under her skin.
Does not the follicle of the eyebrow grows back immediately and with same thickness even if you pluck after an interval (although these plucked hair are partial follicles with various length).
This also proofs that if you pluck a hair follicle(provided it is not plucked from the root), it will regenerate back again and again.
That's why i say we can pluck the follicle every 3 to 4 months and have great supply of hair to convert nw7 to nw1/2 in 2013 itself.
Yes we can improve the quality of plucking by giving eyes to the technique by using special hair ULTRASOUND.
And most importantly we will have to support the partial follicle with dp cells,stemcells and growth factors.
All the above skill and ingredients will define the regenerative % at recipient,
but no way any damage to donor which is safe and intact.
Here the partial graft acts like a semi hair germ with which stemcells and dp cells can talk and repair regrow the follicle.
Thus this becomes the interim solution for MPB total cure till hair germ is successfully developed in lab and is successfully implanted.

Maybe I'm missing your point here, how is a girl plucking hairs related to splitting grafts ?

[gc83uk]

But in your opinion, what exactly causes that 20% of donor in the Gho procedure doesn't regrow ? Did they extract too much of the graft ? You're basically saying that that shouldn't matter ? Or am I missing something here ?

I would hazard a guess it's because only 80% to 90% of hairs are in anagen phase.

[Arashi]

hmmm, didn't you know that -in theory- you can make a complete hair follicle just from 1 single (hair follicle-)cell?? Just from one!

So who cares about any "bisection level" IF you know how to create a brand new complete follicle just from 1 (hair follicle-) cell.

THE reason why most researchers out there always get just "mixed results" from bisection studies is just due to lack of knowledge about what I have just explained... and yeah, so it's not surprising that, of course, they get always better results the more "cells" they extract and implant the greater the success. And the lesser the "mass of cells" the greater the failure - but all that is just one reason why most HT docs still prefer to extract and to transplant "the whole mass" of something - as with heart or kidney transplants ...lol

But in your opinion, what exactly causes that 20% of donor in the Gho procedure doesn't regrow ? Did they extract too much of the graft ? You're basically saying that that shouldn't matter ? Or am I missing something here ?

[534623]

I will prefer to use world's first ultra sound to locate the exact level of bisection...Because i do not have eyes of a scientist like you ,which can give me vision under the skin,and exacatly so because different follicles have different length and different angle...that's why if you follow blind procedure ,you will lower regen %, grow up...!.

hmmm, didn't you know that -in theory- you can make a complete hair follicle just from 1 single (hair follicle-)cell?? Just from one!

So who cares about any "bisection level" IF you know how to create a brand new complete follicle just from 1 (hair follicle-) cell.

THE reason why most researchers out there always get just "mixed results" from bisection studies is just due to lack of knowledge about what I have just explained... and yeah, so it's not surprising that, of course, they get always better results the more "cells" they extract and implant the greater the success. And the lesser the "mass of cells" the greater the failure - but all that is just one reason why most HT docs still prefer to extract and to transplant "the whole mass" of something - as with heart or kidney transplants ...lol

[Arashi]

GC,

does a beautiful girl loses her eyebrow,
although she plucks her eyebrow every month and the eyebrow regrows with similar thickness and with same speed,
Still that's a good point to analyse in coming weeks.

Maybe I'm missing your point here, how is a girl plucking hairs related to splitting grafts ?

[drnigams]

The great scientist ,you can just google and type hair morphometry studies and you will get the author...nothing rocket science like...extraction of stemcells... ..preservation media to activate and multiply follicles...!

By the way - WHO (author) found it out?

Or is that just something you found at hairsite in one of my posts?

[drnigams]

The all knowing great scientist, ironman,
Here we are discussing locating the dermal papilla below the skin and not when it is lying outside the scalp as in your petri dish.
Do you understand the most intelligent researcher mr im.
I will prefer to use world's first ultra sound to locate the exact level of bisection...Because i do not have eyes of a scientist like you ,which can give me vision under the skin,and exacatly so because different follicles have different length and different angle...that's why if you follow blind procedure ,you will lower regen %, grow up...!.
Now i hope the dumbest will understand why it is the only relevant thing in invivo technique.


E=534623;114502]Didn't you know that practically it's IRRELEVANT and NEEDLESS to know that?
The reason why it's irrelevant and needless - oh, THAT's indeed rocket science, because "the exact level" (aka 'perfect level') varies from patient to patient and even from follicle to follicle anyhow...that means, you know a shit anyhow about the "exact level" ...


hmmm, I didn't know that scientists and hair researchers needed various "morphometry studies" to find out "where the root/dermal papilla lies".

Check this out ...

The pic shows a typical Gho-petri-dish with typical Gho-HST-grafts and this pic is taken straight from Dr. Gho's website: http://www.hasci.com/en/rates/
And just in case of any doubts ...

...I couldn't see something different in my OWN petri-dish - could you, gc?

Anyway, the point is:
Even the dumpest hair transplant doctor out there is able to show you in the first pic (with red arrows or something) "where the root/dermal papilla lies" - WITHOUT "morphometry studies"...[/QUOTE]

[534623]

GC,
Percentage is dependent on at the exact level we bisect the follicle.I will not disclose the exact level, niether have i shown that in pics as of now.
As you know the follicle is approx. 4.12mm deep as per various morphometry studies where the root/dermal papilla lies.

By the way - WHO (author) found it out?

Or is that just something you found at hairsite in one of my posts?

[534623]

GC,
Percentage is dependent on at the exact level we bisect the follicle. I will not disclose the exact level, niether have i shown that in pics as of now.

Didn't you know that practically it's IRRELEVANT and NEEDLESS to know that?
The reason why it's irrelevant and needless - oh, THAT's indeed rocket science, because "the exact level" (aka 'perfect level') varies from patient to patient and even from follicle to follicle anyhow (in the same patient!)...that means, you know a shit anyhow about the "exact level" ...

As you know the follicle is approx. 4.12mm deep as per various morphometry studies where the root/dermal papilla lies.

hmmm, I didn't know that scientists and hair researchers needed various "morphometry studies" to find out "where the root/dermal papilla lies".

Check this out ...

The pic shows a typical Gho-petri-dish with typical Gho-HST-grafts and this pic is taken straight from Dr. Gho's website: http://www.hasci.com/en/rates/
And just in case of any doubts ...

...I couldn't see something different in my OWN petri-dish - could you, gc?

Anyway, the point is:
Even the dumpest hair transplant doctor out there is able to show you in the first pic (with red arrows or something) "where the root/dermal papilla lies" - WITHOUT "morphometry studies"...