50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

drnigams · 05-24-2013, 04:52 PM

One member from HRN,who joined before me..who is from mumbai..is slick bald..NW6 should be getting his doubling plus HM ..next month end...
Let's first focus on Tom Vercetti,a forum member and forum coordinator at french forum...results..
Procedure is on monday..and mat be repeat touch up for density on wednesday...

update latest pics...of approx.5000G doubling to 10000G, all at recipient...done on an HT doc..in another thread...in a fortnight..

Plus another guy from malaysia ..is being followed up at another thread..who is around NW6..to be converted to NW2..in coming months..
Regards your suggestion,
I have in mind ..one senior member from canada..who will shoot documentary and great photos..i will be doing at cost to cost for him..he will shortly working on my new website...

May be one day..you will understand..i am not here for bussiness or investment..i would rather invest on photon microscope..and research on HM, as we are still some distance from finding true cure through HM.
As per the visits planned by forum members..we will have one member every month..i think by this year end ..you will have sufficient independent documentation..

UOTE=didi;124868]Dr Nigam

Can you find true slick nw6/7 and give him 5 000-6000 grafts in one session,
shave his head to see if regeneration occurred and recipient will be easy to asses

Give these guys free procedure +accommodation+flights...if success it will be the best investment ever..you know that already...so just DO IT[/QUOTE]

veca · 05-24-2013, 04:35 PM

That is what i am talking about, great Didi !!! Dr. Nigam just do that for us...

santos · 05-24-2013, 04:33 PM

Originally posted by Arashi

Not sure what happened Didi but lately i'm agreeing a lot to what you say

This indeed would be a GREAT way to show us proof of your technique, Dr Nigams.

I think its because we all want his "Dr.Nigams" procedure to work as claimed... so we all come together.

Arashi · 05-24-2013, 04:23 PM

Originally posted by didi

Dr Nigam

Can you find true slick nw6/7 and give him 5 000-6000 grafts in one session,
shave his head to see if regeneration occurred and recipient will be easy to asses

Give these guys free procedure +accommodation+flights...if success it will be the best investment ever..you know that already...so just DO IT

Not sure what happened Didi but lately i'm agreeing a lot to what you say

This indeed would be a GREAT way to show us proof of your technique, Dr Nigams.

didi · 05-24-2013, 04:21 PM

Dr Nigam

Can you find true slick nw6/7 and give him 5 000-6000 grafts in one session,
shave his head to see if regeneration occurred and recipient will be easy to asses

Give these guys free procedure +accommodation+flights...if success it will be the best investment ever..you know that already...so just DO IT

veca · 05-24-2013, 01:23 PM

The results are impressive indeed, but we still have not seen a classic transformation NW7 to NW1-2 ...
Dr. Nigam
Can you find one forum member who NW7 and turn gau NW1-2? To conclude, once and for all whether you are able to really achieve! Everything revolves around the theory and the "small" evidence. I do not want to be negative, to the contrary, I am very excited about the results but before I sit on a plane to Mumbai I want to see this transformation ...

One · 05-24-2013, 12:08 PM

Dr Nigam what do you pay to user of forum your in vitro technique?

oppenheimer82 · 05-24-2013, 10:48 AM

keep it coming dr nigam. very nice results indeed!

FearTheLoss · 05-24-2013, 10:35 AM

yeah, I mean it's your decision, but be careful.

TO YOUNG TO RETIRE · 05-24-2013, 10:22 AM

so some of us must get there and document this, bring it to the news etc,

FearTheLoss · 05-24-2013, 10:20 AM

Lol, you guys need to relax until things are proven. There are so many scammers in the hair loss industry, obviously you guys are new members so you haven't learned this and done your research yet. (not saying whether Nigam is a scammer or not, I would like to believe he's the real deal) You guys just need to calm yourselves and wait for proof before getting all excited and jumping to conclusions.

TO YOUNG TO RETIRE · 05-24-2013, 10:09 AM

we must make a donate page and donate him for his research, lets all help curing this shit...

i mean really this guy is going to get kidnapped with the things he is achieving, respect

Gjm127 · 05-24-2013, 10:07 AM

The dude's a hustler, never seen this before! Props to you Dr. Nigam.

Thank you!

TO YOUNG TO RETIRE · 05-24-2013, 09:48 AM

this is unbelievable, please Dr give us proof of large sessions of 4k grafts of that kind

drnigams · 05-24-2013, 08:53 AM

Day 53 Update In-Vitro Doubling of 15 G patch test @Dr. Nigam's

Day 53 Update In-Vitro Doubling of 15 G patch test @Dr. Nigam's

Dear friends,

Kindly find Day 53 update of donor and recipient of 15 graft patch test of in-vitro hair doubling along with tattoos marks and one shot video.

results
Donor - On Day 0 total 23 follicles were extracted from 15 grafts (Single - 8, Double - 6 & Triple - 1)

On Day 53 by Videoscopic & Macro images of Donor shows - 37 follicles as against 52 follicles on Day 18 may be some must have gone into shedding or some may have not regenerated. Although still better than 23 follicles which were extracted from the donor which mean the gain of 14 follicles at the donor which means as on today (Day 53) regeneration is 160%, may become less or more, actual donor regeneration will come to know in 6 to 9 months. You must be wondering how come more than 100% regeneration that is becuase of stem cells, dp cell, growth factor were injected into the donor and recipient. You can clearly see even after using large punch to extract the graft from the donor like in FUE...... THERE IS NO WHITE DOT OR SCAR MARK. This proves if FUE doctors start using ECM, concentrated PRP and / or growth factor, stem cells, DP cells, visible FUE scar can be avoided. As the above have important role in wound healing. Hence no white dot or scare.

Recipient shows 23 follicles as against 38 follicles on Day 18 but still 100% recipient regeneration as 23 bisected follicle were implanted at the recipient, may be the 15 follicles is not seen on Day 53. May be they must have gone into shedding or they must have not regenerated. Actual recipient regeneration will be known after 6 to 8 month.

observe the pigmentation and thickness of Donor and Recipient re-growth to analyze the diameter of new hair.

WCHR 2013, Edinburgh, UK, had 2 paper presentation on unlimited hair transplant. One Japanese paper were shown which was earlier confirmed by Jahoda that ........
Freshly isolated DP cells which is trichogenic, if implanted at the base of upper half of the amputated / bisected follicles, new dermal papilla or new follicle with similar characteristic of the donor will be created.
Another study confirm that upper half of the hair follicle if amputed and extracted with outer root sheath stem cells minimum and great if extracted with matrix stem cells can create it's own dermal papilla.

The study also confirmed that the lower dermal papilla when imputed from its upper half of the follicle can create it's own shaft and rest of the follicle.

Cotsarelis had published one paper called "De-Differentiation of stem cells" in which bulge stem cells (epidermal) can become matrix germ stem cells and dermal stem cells and similarly dermal stem cells can become epidermal stem cells if required, this is called stem cell De-Differentiation.

With my experience of 4 to 5 month of in-vitro and in-vivo technique, as on today I come to conclusion that in-vitro hair doubling or donor doubling will give higher donor & recipient regeneration with better quality of hair than in-vivo donor doubling.

since in in-vivo donor doubling the major issues is that its a blind technique and not one can bisect the follicle at the matrix germ cells level or dermal cup sheath level. In-vivo technique has disadvantages....
1. since it's a blind technique, precise bisection which we want cannot happen
2. Becuase precise bisection cannot happen some extraction cannot suitable hence multiple extraction need to be done
3. Because it is blind technique hence it will be more expensive
4. The hair re-growth at the recipient will be much lesser than the in-vitro technique because only part of upper follicle is being extracted hence hair at the recipient could be thinner.

Both In-vitro and in-vivo technique will be scarless but the role of injecting stem cells, dp cells, growth factor, ECM, as per my observation is very important for donor and recipient re-growth and thickness of hair.

I remember what Washneik said that in the 1st phase hair multiplication will be used as a adjunct to the hair transplant. This is what I feel too, by the time in coming months we will get consistent result when we will add 3d spheroidal aggregated capsulated, growth factor secretarine trichogenic, good dose of dp cells and / or what Dr. Gerd and Tsuji Lab did that is co-culturing of epithelial stem cells and trichogenic dp cells to create proto hair or micro follicle as named by Gerd for real CELLULAR HAIR IMPLANT or if Histogen succeeds and as believed by Ralf Paus of UK as he told to me in WCHR 2013 that dormant 100000 hair follicles lying also in the slick bald scalp in Telogen phase with partial fibrosis can be activated with stem cells, DP cells, Growth factors, Noggin, WNT7A, etc. in-vivo.

I would advice all the patient of AGA doing or not doing hair transplant, doubling or HM should start with mast cells supressor (Oral or topical) OC-03-01, anti-androgen preferably topical will come in the market, antioxydent like melatonin. Oral good dose of antioxidents and smoker should start L-Cystine and biotin in good doses orally and / or topically to counter the follicle damage of nicotine.

By next month I will have topical vascular endothelial growth factor FGF and others which can be applied topically for the medical AGA prescription but these topical gels are not available to the patient directly but only to the bio-tech labs.
For prevention of AGA I am working on how to reduce on micro inflammation due to multiplication at and around hair follicle and androgenic TGF - Beta-catenine topical or injectible to reduce mast cell released histamines. Reducing DHT concentration on scalp, countering prostaglandin, interlukine 1 & 6 concentration on scalp and products to up regulate PGE2, insulin growth factor, products to prevent apoptosis of stem cells, products to convert dormant stem cells to progenitor stem cells in-vivo through ioantophoresis, electoporation, nano particulate transdermal targetted delivery, chitosan, etc capsulated vehicle.

These medical prescription will also helps my HM results if patient starts this medical prescription, same for the regular transplant.

If I had movable photon microscope costing US $1 million, my HM result would have increase significantly. Since with movable photon microscope applied on patient scalp. I can see telogen follicles on the human scalp and inject the stem cells directly into it specially when they are fibrosed, partially or totally, normal stem cells injection could be difficult to them. In movable photo microscope, I can see cells of epidermis and dermis and also upward & downward movement of stem cells.

As you remember I had mentioned the use of photon micro-scope in cellular regeneration micro imaging study, same was highlighted by 2 presenters with their paper titled "Imaging in hair generative therapies"

Elian Fuchs in her paper presentation "Stem cells in silence and in action" showed upward & downward descent video of moving stem cells in a hair follicle in-vivo

Hope fully I have to do some research in Gerd lab in Berlin which is having fixed photon microscope and bio-reactors, etc to find the answer that why HM doesn't response on all the scalp? and we know HM respond on some scalp and in these responding scalp the results are really good and acceptable.

Donor Count line

Click the below link for enlarge image

http://www.drnigams.net/images/RTN/Day53/Large/Donor/Donor-countline.jpg

Recipient IMG

Click the below link for enlarge image

http://www.drnigams.net/images/RTN/Day53/Large/Recipient/Recipient.jpg