The 50 Graft Test Procedure

[Arashi]

Ok, great news guys, got mail back from Kristel and confirmed what I've said before !!

So basically, the cut-off hairs you see are just that: Cut-off hairs. Dead material. The graft stayed in the donor for these hairs and thus these are expected to regrow 100%.

The hairs WITH bulb will get transplanted and these are expected to regrow 80%.

So, in our example: let's say we find 400 hairs in the petridish. 250 hairs are usuable according to HASCI (we still have to verify but I'm pretty damn sure we'll find 250 hairs with bulbs in the petridish). Hence in donor we should expect this amount of hair growing from the extraction sites: 150+ 0.8*250= 350 hairs. So 350 out of the 400 hairs should regrow. Hence James should have lost about 50 hairs in donor and gained about 250 new hairs in recipient.

[c5000]

Here's my take on everything up to this point:

- I haven't counted the number of hairs in the '1' subsection yet. For this post, I'll assume that there's around 400-450 total hairs in the petri dish like Arashi said. I'll verify this later though to make sure.

- According to HASCI, they extracted 150 usable singles and 50 usable doubles. If we accept this as true, this implies that there are 250 usable hairs in total. To confirm this, perhaps we can count the number of hairs with intact bulbs in the petri.

- If there are 400-450 total hairs and 250 of those hairs are usable, this implies that the other 150-200 hairs are either transections or in telogen. Based on the numbers, it's clear that the majority of these must be transections. These transections should hopefully regenerate in the donor but are unlikely to yield in the recipient. For the telogen hairs, these are unlikely to regenerate in the donor. It's unsure if they will yield in the recipient either.

- When calculating the donor regeneration, we should reference this with the 400-450 extracted. When calculating the recipient yield, we should reference this with the 250 usable grafts.

- The most important thing we should look at is the net gain in hairs. We started out with 400-450 in the petri, let's see how many we end up with when we count the hairs in the donor and recipient later. This is the simplest proof of concept.

Will they even put these unusable grafts/hairs in the recipient though?

[didi]

Originally Posted by Arashi
Anyway, I'm sure we'll get an answer from her tomorrow regarding the bulb-less hairs 'mystery'. Like said my guess is that she'll say that ALL bulb-less hairs will regrow and 80% of the hairs with bulb will regrow too. I'd be highly surprised if she doesn't say that.




Look at the pic of HST grafts, has bulb, means HST graft needs bulb to take - in recipient
bulbless hairs are expected to regrow in donor(80%)

Those with bulbs will regrow in recipient...almost total regrowth as Ghos says it in the pic

[didi]

arashi

can you elaborate when you say all bulbless hairs an hairs will bulbs will regrow...

do you mean 2 hair graft will regrow in donor and recipient (real doubling)or 1 hair(bulb) will grow in donor and other bulb-less in recipient or something like that ?

[FearTheLoss]

I feel like this test we did won't prove much to anyone..the critics will still be critics because we are still guesstimating things..

[JJJJrS]

I'm pretty sure I've overcounted and am going to say there are 390-420 hairs in the petridish. Need some time to really sit down for it but I think this will turn out to be true.

I'll confirm it eventually to make sure. But my impression is that most people will have a tendency to undercount the results.

[Arashi]

The majority of these bulb-less hairs must be transections.

For sure. Anyway, ttyl, hopefully with some good news from her

[JJJJrS]

Anyway, I'm sure we'll get an answer from her tomorrow regarding the bulb-less hairs 'mystery'. Like said my guess is that she'll say that ALL bulb-less hairs will regrow and 80% of the hairs with bulb will regrow too. I'd be highly surprised if she doesn't say that.

I totally agree with that and would also be shocked if she doesn't say the same.

The majority of these bulb-less hairs must be transections. I'm certain of that and no other explanation makes sense.

The nice thing is, we'll be able to see if this explanation is correct in a couple of weeks when the donor starts to regenerate.

I wish they had kept it at 50 grafts though. Counting 400-450 hairs won't be fun at all

[Arashi]

- If there are 400-450 total hairs

I'm pretty sure I've overcounted and am going to say there are 390-420 hairs in the petridish. Need some time to really sit down for it but I think this will turn out to be true.

[Arashi]

- According to HASCI, they extracted 150 usable singles and 50 usable doubles. If we accept this as true, this implies that there are 250 usable hairs in total. To confirm this, perhaps we can count the number of hairs with intact bulbs in the petri.

Haven't counted but at first glance I'm pretty sure this will turn out to be true.

About the HASCI made photo's: Kristel said in Dutch they've made "mis oecologische" recordings, which will be released.

Not sure what "mis oecologische"even means, but oecologische would probably translate as "ecologic" ? Maybe someone knows what she's saying here ?

Anyway, I'm sure we'll get an answer from her tomorrow regarding the bulb-less hairs 'mystery'. Like said my guess is that she'll say that ALL bulb-less hairs will regrow and 80% of the hairs with bulb will regrow too. I'd be highly surprised if she doesn't say that.

[JJJJrS]

Here's my take on everything up to this point:

- I haven't counted the number of hairs in the '1' subsection yet. For this post, I'll assume that there's around 400-450 total hairs in the petri dish like Arashi said. I'll verify this later though to make sure.

- According to HASCI, they extracted 150 usable singles and 50 usable doubles. If we accept this as true, this implies that there are 250 usable hairs in total. To confirm this, perhaps we can count the number of hairs with intact bulbs in the petri.

- If there are 400-450 total hairs and 250 of those hairs are usable, this implies that the other 150-200 hairs are either transections or in telogen. Based on the numbers, it's clear that the majority of these must be transections. These transections should hopefully regenerate in the donor but are unlikely to yield in the recipient. For the telogen hairs, these are unlikely to regenerate in the donor. It's unsure if they will yield in the recipient either.

- When calculating the donor regeneration, we should reference this with the 400-450 extracted. When calculating the recipient yield, we should reference this with the 250 usable grafts.

- The most important thing we should look at is the net gain in hairs. We started out with 400-450 in the petri, let's see how many we end up with when we count the hairs in the donor and recipient later. This is the simplest proof of concept.

[JJJJrS]

Anyway, good news is she said that they've been taking their own pictures as well, which are still to follow ! So we'll hopefully get better pictures for analysis.

Just to be clear, the pictures that james posted weren't from HASCI?

Hopefully they took some nice, high quality photos because that would make the analysis much easier.

The petri dish photo is great though and certainly very informative.

[Arashi]

I wonder what Dr Nigam thinks about all of this?

Haha seriously ? After that picture of NSN he posted with all those circles he made up ? He obviousy has no interest in the truth, just like Didi.

[gc83uk]

I wonder what Dr Nigam thinks about all of this?

[Arashi]

lol, to be honest I reckon Iron Man thinks we are all idiots who doesn't know anything and he is the only normal person here.

Haha Actually I'm SURE he thinks that