New Stemcell Treatment Photos... wow?

[StinkySmurf]

Its good to see that you are also going to experiment with DP culture in medium that preserves its hair inducing ability after more doublings, If you manage to do this right like in the current studies, it would be a clever improvement over what currently is available on the market.




Good luck with it.

Aderans solved this problem didn't they. They can maintain hair inducing ability for as many cycles as they want now right?

Dr. Nigam,

I meant to ask did you encounter problems maintaining the inducing ability at 3 months. Is this why you originally started at 18 days or is it just cheaper cost and an easier domestic sales pitch?

Thanks!

[Boldy]

Dr gho or anyone cannot see DP or DS,since it's a blind technique ,they can only with understanding of hair morphometry and guess with experience and may try to extract a part of FU which may contain some part of DP and or DS cells.Ideally the disection at the levelof aubers line would be the best option .

Its good to see that you are also going to experiment with DP culture in medium that preserves its hair inducing ability after more doublings, If you manage to do this right like in the current studies, it would be a clever improvement over what currently is available on the market.

each subsequent passage. After passage two, each sample had undergone an average of 15.3 doublings, approximately a 40,000-fold expansion in cell number. At this point, cultures were still
capable of rapid growth. We have not performed an extensive study of culture growth to senescence, but some cultures have been passaged for more than 50 doublings without a significant reduction in the growth rate

https://www.dropbox.com/s/9doxfq9hqny8olc/8%20hair%20folucle%20newogenesis%20induced%20by%20cultured%20human%20scalp%20dermal%20papilla%20cells.pdf

Good luck with it.

[neversaynever]

Dr gho or anyone cannot see DP or DS,since it's a blind technique ,they can only with understanding of hair morphometry and guess with experience and may try to extract a part of FU which may contain some part of DP and or DS cells.Ideally the disection at the levelof aubers line would be the best option .

Good. Do it Dr Nigam! Make us hairy Looking forward to seeing more doubling case studies. I cant see why you would not get growth in donor and receipient. Really hope you present us case studies thatll convince us all.

One thing I'm curious about how many grafts max you can extract? Whats the highest youve done so far for doubling? Or what would you hope will be the highest? Are their dangers damaging donor regrowth if you take too many grafts? Im really hoping it works, because it shouldnt be just Gho doing this technique, and his patent doesnt apply to india.

[drnigams]

Dr gho or anyone cannot see DP or DS,since it's a blind technique ,they can only with understanding of hair morphometry and guess with experience and may try to extract a part of FU which may contain some part of DP and or DS cells.Ideally the disection at the levelof aubers line would be the best option .

Gho's procedure leaves the bottom side of the hairfolicle intact in the donor area.. if i'm not mistaken! and that part contains the dp.

[drnigams]

in vitro bisection not in vivo

Boldy is a very positive development to the thread.
You are right boldy ,my recent experience with last to case studies show more than 100% growth after in vivo bisection from the level of aubers line with 50x magnification.Two other interesting thing i did was...after bisecting 2 follicle FU ,i got two sets of DP cells and two sets of 2/3rd follicle above aubers line probably with DS.
Each bisected new FU had one set of DP cells and 2/3rd part of follicle.
I also added activated progenitor stemcells into both the bisected units.Excellent results in the first two case studies with more than 100% growth in a week.


its very very promising, it s Mather of time for the trials to finish so they can offer this procedure on the market.. after the fda approval etc.

the good part about this, the procedure is fully described in their studies/papers, a person who can read studies, and has access to a lab, can culture these cells, and then....

[/QUOTE]

[drnigams]

Only thing is that dr ghos technique improves if he injects progenitor stem cells into the donor and bisected follicles.
His technique will improve if he start injecting DP cultured potent cells into both the bisected FU.
And his technique will improve if he starts bisecting HF in vitro under high magnification.9not blindly in vivo)
Regarding DP and DS cell culturing we are expirementing with vd3,gsk3inhibitor ,wnt 10a and other enriched media to culture without DP cells losing there inductive potency.
Lauster team has claimed to have successfully cultured DP cells and DS cells,will make my DP culture more effective with their expertise and experience,as we are meeting on 27th jan.

there is for sure truth in your story, but that means, DR. Ghos doubeling would not work either over long term, right?

than how is it possible that it does work.....

It is, because regarding this paper from dr cotseralis:

https://www.dropbox.com/s/lni0awqestf5x73/13%20Review%20of%20hair%20follicle%20dermal%20cells.pdf

If you get a Dermal sheeth from a donor area and impant it into the host (aga are), It will recreate a dermal papilla. So its posible what DR nigams is saying, regarding the studies.







Now whats the different between the DP cells in the AGA area and outside the AGA area?:




With other words, Culturing, or transplanting the Dermal sheeth or dermal papilla cells, would lead to an immortal hairfolicle, since it can't destroy it self.


I can fully imagine that you guys are sceptic (you have your reasons for that Pictures etc that probably are not true on his site),But I rather stay Objective and focus on the facts/ studies that are proven to work in practice (Gho), and the papers regarding the dp and ds cells.



the problem with the Dermal papilla cells in the AGA area is that the are affected by DHT, which unfortunatley lead in to shutdown important pathways, like wnt, igf, shh bmps etc. Its a chain reaction of processes. apoptosis + catagen. Indeed the stemcels are perfectly intact in the AGA area, but they miss cd200+cd34+ progenitor cells. (explainable because of the chain reaction). This is good that the stemcells are still intact, since we now only have to implant or inject DP/ ds cells that are not affected by DHT), and an immortal hairfolicle could develop.

aderansresearch has created and tweaked the DP culture process in, and now seem to achieve 70% success rate, which is very promising!.

http://www.aderansresearch.com/

Regarding what I understand from nigams site, is that they culture the cells in the Dermal sheeth, and bulge, and then inject them into the scalp. If this is done right, healthy DP cells/ follicles could develop.



BTW guys, I'm Not supporting anyone here, Just focussing on the theory they seem to use.. On paper it seem to fits (so far)

The Dp/Ds culture thing got my interest lately, since I know its the only way that will give me my old nw0 back.

For your information, I'm GansterBoy on hairlosshelp.

[drnigams]

Boldy is a very positive development to the thread.
You are right boldy ,my recent experience with last to case studies show more than 100% growth after in vivo bisection from the level of aubers line with 50x magnification.Two other interesting thing i did was...after bisecting 2 follicle FU ,i got two sets of DP cells and two sets of 2/3rd follicle above aubers line probably with DS.
Each bisected new FU had one set of DP cells and 2/3rd part of follicle.
I also added activated progenitor stemcells into both the bisected units.Excellent results in the first two case studies with more than 100% growth in a week.


its very very promising, it s Mather of time for the trials to finish so they can offer this procedure on the market.. after the fda approval etc.

the good part about this, the procedure is fully described in their studies/papers, a person who can read studies, and has access to a lab, can culture these cells, and then....





[/QUOTE]

[garethbale]

Look, please ignore 534623. Hes mentally ill (im not joking) and a complete child. Lets get back on topic, no matter how much I enjoy watching a spurs fan getting abuse

haha, yeah cheers for that neversay never

Yea aderans lookings a bit promising, but Ive yet to see anything amazing from them. Only single injections at this point with some regrowth. But their progress and attitude are brilliant.

I'm still unsure over Aderans. They seem forthcoming and professional but I talke whatever Ken Washenik says with a pinch of salt. I remember seeing a video of him in 2008 saying HM would be available by late 2009/early 2010. He just seems a good PR man...

[neversaynever]

https://www.youtube.com/watch?v=2oQYjppOPJM


goodluck

its very very very promising, it s Mather of waiting for the trials to finish so they can offer this procedure in the market..

the good part about this, the procedure is fully described in their studies, a person who can read studies, and has a lab, can culture these cells, and then....

Yea aderans lookings a bit promising, but Ive yet to see anything amazing from them. Only single injections at this point with some regrowth. But their progress and attitude are brilliant.

We'll know more this year (as they start phase 3 i hope!). Didnt aderans say they are not creating new follicles?

And i believe that 70% is now 60%.

[neversaynever]

Look, please ignore 534623. Hes mentally ill (im not joking) and a complete child. Lets get back on topic, no matter how much I enjoy watching a spurs fan getting abuse

Gangster boy, please stick around. I dont have much knowledge so itd be nice if you kept an eye on this thread.

Everyone stop replying to him, block his posts if possible. He ruins every thread with that attitude.

And yes, gangster...please elaborate about the 70% thing?

At the moment Nigam is trying the doubling technique, its not rocket science, and cant see why it'd fail. Waiting for updates from the doc.

[Boldy]

Can you provide a link to something with that 70% stat, and please don't stop posting because it's good stuff?

https://www.youtube.com/watch?v=2oQYjppOPJM

goodluck

its very very promising, it s Mather of time for the trials to finish so they can offer this procedure on the market.. after the fda approval etc.

the good part about this, the procedure is fully described in their studies/papers, a person who can read studies, and has access to a lab, can culture these cells, and then....

[534623]

Are you intentionally addressing me? What did I do/say?

He is saying that you're talking bollocks.

[garethbale]

I'm not sure how its possible that the admin, allow such people like you on this forum..

you are going to far.

anyway, this is a lost conversation, I hoped to get a normal discussion here about the facts and the papers/studies, but it seem no one reads studies here. It makes no sense.

Its Big waste of time!

Are you intentionally addressing me? What did I do/say?

I had a go at 534 for having a go at you!

[StinkySmurf]

I'm curious how you get that information, do you have a source?, the trials and studies Claim that it Is possible to recreate immortal hairfolicles from Human Dermal papilla , or Dermal sheeth cells... and that the success rate currently is 70%.

Can you provide a link to something with that 70% stat, and please don't stop posting because it's good stuff?

[Boldy]

you're talking bollocks again...

I'm not sure how its possible that the admin, allow such people like you on this forum..

you are going to far.

anyway, this is a lost conversation, I hoped to get a normal discussion here about the facts and the papers/studies, but it seem no one reads studies here. It makes no sense.

Its Big waste of time!