adipose-derived stem cell protein extract

[hiko]

There were quite some presentations about it at the hair congress too;

P080
HAIR GROWTH STIMULATION BY ADIPOSE TISSUE-DERIVED STEM CELLS
Gyeong-Hun PARK

* P153 (FC4) ADIPOSE DERIVED STEM CELLS AND GROWTH FACTORS APPLIED ON HAIR TRANSPLANTATION. FOLLOW-UP OF CLINICAL OUTCOME
Federica ZANZOTTERA

P222 STUDY ON HAIR-INDUCING CAPACITY OF HUMAN ADIPOSE-DERIVED STEM CELLS (ADSC)
Chang Hoon SEO

P231 CLINICAL USE OF CONDITIONED MEDIA OF ADIPOSE TISSUE-DERIVED STEM CELLS IN FEMALE PATTERN HAIR LOSS: A RETROSPECTIVE CASE SERIES STUDY
Hyoseung SHIN


So i guess it surely grows SOME hair.. The interest is there.

The results in the published report show more than just "some" hair growth. AFAIK, there aren't current or pending treatments that have shown results that good.

These results should hopefully apply to Histogen's phase 2b higher dosing trials. They use the same core ingredients.

[Swooping]

The results in the published report show more than just "some" hair growth. AFAIK, there aren't current or pending treatments that have shown results that good.

These results should hopefully apply to Histogen's phase 2b higher dosing trials. They use the same core ingredients.

I agree, what would be nice if we could obtain it at a low price and actually do more sessions. That would probably yield even better results too?

[nameless]

I agree, what would be nice if we could obtain it at a low price and actually do more sessions. That would probably yield even better results too?

Swooping I think that the difficulty we're having getting into communication with the folks in that study is a positive sign. They are not in any hurry to talk with us but snake-oilers usually email back immediately after I contact them because they want to get the money quickly. I found one of the scientists email address in the study and I emailed him 4 or 5 days ago and he still has not responded PLUS they haven't responded to you or the other poster who tried to contact them. I think this shows they are in no rush to take our money. Based on this I think there is a good probability that, that study is legitimate. I really want to try the treatment that was in that study but I want to do it exactly as it was done in that study. I live in America but I would fly back and forth to them for treatment. I do not want to bother with a treatment that is close or similar to what was in the study. I want the exact same treatment. If you find a way to get me in touch with the people in the study please tell me how.

[Alias123]

if anyone knows any place in the EU who does or are going to this treatment in the near futureplease link!

[moleular]

http://ddrheinrich.com/en/treatments/face-skin-hair/stem-cells/

I've been in contact with them. They offer ADSC's and their respective growth factors.
They aren't cultured in hypoxic conditions, as they were in the research at the start of this thread.
Culturing stem cells for therapy (according to them) is currently not allowed under EU law, although they have mentioned that it is possible via their Swiss lab.
Their basic ADSC procedure is €5000.
I'm awaiting some answers to various things, but will keep you posted.

[Alias123]

Thank you moleular! i will look into it and possible do a treatment within a month, have no financial boundries so i might aswell take a chance and try it, if they check out that is, keep me posted! thanks again

[nliyan25]

Anybody?

Is anybody considering buying this? If this works, I would definitely fork over $1000 for it.

http://ymsconsulting.org/aape_hair.html

[moleular]

I saw it and contacted the guy.
He offered very little information in response to my questions, and pointed me to a really poor wix site for answers.
I should imagine it's either not the real thing, or he gets it through back door channels.
Having bought real growth factors from life science companies, I know that they can't just be kept refrigerated as he suggested, and if they are, they need to be used rapidly to maintain a biological response. They also need to be shipped in icepacks and with a rapid shipping service.

Having said that, I think it needs probing more, and really pin him down on the source. It could well come from the korean company which make it, but he's probably going to be reluctant to say much, given that it won't be fda approved

[nliyan25]

good point

I saw it and contacted the guy.
He offered very little information in response to my questions, and pointed me to a really poor wix site for answers.
I should imagine it's either not the real thing, or he gets it through back door channels.
Having bought real growth factors from life science companies, I know that they can't just be kept refrigerated as he suggested, and if they are, they need to be used rapidly to maintain a biological response. They also need to be shipped in icepacks and with a rapid shipping service.

Having said that, I think it needs probing more, and really pin him down on the source. It could well come from the korean company which make it, but he's probably going to be reluctant to say much, given that it won't be fda approved

Good point, let's hope it's the real thing.

[Swooping]

http://ddrheinrich.com/en/treatments...ir/stem-cells/

I've been in contact with them. They offer ADSC's and their respective growth factors.
They aren't cultured in hypoxic conditions, as they were in the research at the start of this thread.
Culturing stem cells for therapy (according to them) is currently not allowed under EU law, although they have mentioned that it is possible via their Swiss lab.
Their basic ADSC procedure is €5000.
I'm awaiting some answers to various things, but will keep you posted.

Great man. What is their method then? They extract it, seperate it and immediately inject it or what? Btw, how hard is it do yourself? Is there anyone here with lab experience, obviously you would need the equipment etc. But seems like it is rather easy? 5000 euro is alot, very curious what it will cost through this swiss lab.

[bananana]

Great man. What is their method then? They extract it, seperate it and immediately inject it or what? Btw, how hard is it do yourself? Is there anyone here with lab experience, obviously you would need the equipment etc. But seems like it is rather easy? 5000 euro is alot, very curious what it will cost through this swiss lab.

Yeah.
5k euro is a huge price, how long can there results last?

[moleular]

With regard to adipose extraction method, it isn't a straightforward process.
It will be something like the following;

http://www.adistem.com/technology/adipose-derived-adult-stem-cells/

It seems adistem have chosen to use PRP as the sort of 'carrier'. I don't know whether they use that at the clinic, but it would need some sort of liquid carrier to make the most of the harvested stem cells from the adipose tissue, and probably simply provide enough liquid for injection.

Interestingly, when PRP comes into contact with collagen, it is then activated and the growth factors present within it, increase in their potency.
This is a good and bad thing, as TGF-ß1 is also increased, which is in fact a hinderer of hair growth. It's part of the reason why standard PRP isn't very effective when injected standalone.
Using ACell or other extracellular matrix helps to combat this to some degree by giving the stem cells present in the plasma a scaffold to grow.

I should also note this isn't something you'd want to do at home. I have actually tried to inject things into my scalp and it hurts A LOT.
Typically such therapies are provided using mesotherapy techniques such as nappage or other shallow intradermal injections.
It takes a little skill to get it down properly.

[Swooping]

One method of prostemics is described as;

Human subcutaneous adipose tissues were obtained from 23 healthy women with informed consents as approved by the institutional review boards by medical liposuction. The age distribution of the patients ranged from 26 to 51 years, with a mean of 36.7 years and about 67 kg. The adipose tissues were exposed to collagenase (final 0.075% type II collagenase, Sigma-Aldrich, St. Louis, MO, USA) for 30 min at culture temperature, followed by centrifugation at 400 g for 10 min, washed and resuspended in PBS. The stromal cell fraction was filtered through a 70 μm cell strainer (BD Biosciences, San Jose, CA, USA). Using Histopaque-1077 (Sigma-Aldrich, St. Louis, MO, USA), ADSCs were isolated from the filtrate, then cultured at 37 °C, 5% CO2 in DMEM containing 10% FBS. Characteristic expressions of stem cell-related surface markers were confirmed by flow cytometry [9,10]. ADSCs expressed CD73, CD90 and CD105, and were lacking in CD34 and CD49d. Adipogenic, osteogenic, and chondrogenic differentiation was also checked by the conventional method [43,44]. After the isolation of ADSC from patients, cells were pooled. ADSCs were cultured and expanded in normal control medium, and used for the experiments at passages 4. Cells were finally frozen in aliquots using CellFreezerTM (Genenmed, Seoul, Korea) for the future. To produce a ADSC-CM (AAPETM), a frozen vial containing 1 × 106 cells were launched onto culture medium containing 10% FBS. After repeating subcultures to reach 5 × 108 cells, the expanded ADSCs were introduced into CellFactoryTM CF10 (Nunc, Rochester, NY, USA) in DMEM/F12 serum-free medium (Welgene, Taegu, Korea). Cultures were conducted under a hypoxia by providing 2% O2 using N2 gas supply in a humidified multichannel incubator during 2 weeks. The conditioned media were collected and micro-filtered, followed by quantitated total protein production. Finally, for fresh use, 4 mL vials containing equal protein concentration were freeze-dried as a single lot sample preparation of AAPE (Prostemics Research Institute, Sungnam, Korea) for this study.

We need those I am pretty sure as seen in the movie from 2:21 to 2.35;

https://www.youtube.com/watch?v=vrz3Rp4JwDM#t=154

Are they these;

http://www.prostemics.com/magazine/aape.php

I guess so... Like someone said in this topic the ones for $1000.. Probably way cheaper if directly bought from prostemics though.

[moleular]

I doubt that it could be bought much cheaper from Prostemics themselves. This is what makes me a little uncertain about yms offering it for $1000.
It's typically very expensive to buy growth factors. For example, buying just one growth factor like FGF9 is in the region of $250, and that's only for 20 µg (micrograms).
It should be noted that AAPE isn't quite the same as ADSC.
AAPE is solely the proteins / growth factors which are extracted, probably from a cultured medium containing the stem cells. It is the stem cells which secrete these growth factors. Stem cells are not contained in AAPE.
The ADSC method which started this thread, is a method by which both the stem cells and their respective secreted growth factors are used.
And the difference between the aforementioned and adipose derived stem cells used by Dr Heinrich, is that the stem cells from adipose tissue (your own fat) haven't (and can't by EU law) be cultured.

All of that aside, I really wish people would get behind this method on this forum (particularly those frequently browsing cutting edge treatments), because this is actually a reality now, and can be effective.

I propose community funding to work with Dr Heinrich for example (although it doesn't have to be [and I have no affiliations with his clinic]), and getting something set up whereby we can bring down the price of such treatments.

You all know that the other treatments discussed in this section are at least 2 years away from being commercial, so why not dedicate some proper efforts to something which is doable now.
Community funding for ADSC could almost help to subsidise the treatments of yourself and others.

Admittedly, the better results are from Fukuoka's research whereby he cultured the cells in hypoxic conditions, but with enough money backing it, there's no reason we can't gradually bring that around.

Frankly, I'm sick and tired of playing the waiting game, and I'm not prepared to anymore.

I welcome your thoughts on it, but please don't let it degrade into name calling and veering off at wild tangents.

[Swooping]

I doubt that it could be bought much cheaper from Prostemics themselves. This is what makes me a little uncertain about yms offering it for $1000.
It's typically very expensive to buy growth factors. For example, buying just one growth factor like FGF9 is in the region of $250, and that's only for 20 µg (micrograms).
It should be noted that AAPE isn't quite the same as ADSC.
AAPE is solely the proteins / growth factors which are extracted, probably from a cultured medium containing the stem cells. It is the stem cells which secrete these growth factors. Stem cells are not contained in AAPE.
The ADSC method which started this thread, is a method by which both the stem cells and their respective secreted growth factors are used.
And the difference between the aforementioned and adipose derived stem cells used by Dr Heinrich, is that the stem cells from adipose tissue (your own fat) haven't (and can't by EU law) be cultured.

All of that aside, I really wish people would get behind this method on this forum (particularly those frequently browsing cutting edge treatments), because this is actually a reality now, and can be effective.

I propose community funding to work with Dr Heinrich for example (although it doesn't have to be [and I have no affiliations with his clinic]), and getting something set up whereby we can bring down the price of such treatments.

You all know that the other treatments discussed in this section are at least 2 years away from being commercial, so why not dedicate some proper efforts to something which is doable now.
Community funding for ADSC could almost help to subsidise the treatments of yourself and others.

Admittedly, the better results are from Fukuoka's research whereby he cultured the cells in hypoxic conditions, but with enough money backing it, there's no reason we can't gradually bring that around.

Frankly, I'm sick and tired of playing the waiting game, and I'm not prepared to anymore.

I welcome your thoughts on it, but please don't let it degrade into name calling and veering off at wild tangents.

Yes i know, most of them are derived from e.coli and they don't work though. I think we should have the ones which are cultured under hypoxic conditions as growth factor secretion will get way bigger because of that. I doubt too that prostemics will offer them for way cheaper than $1000, but we'll see.

Its main components of prostemics AAPE;

include PDGF (44.41 ± 2.56 pg/mL), bFGF (131.35
± 30.31 pg/mL), KGF (86.28 ± 20.33 pg/mL), TGF-β1
(103.33 ± 1.70 pg/mL), HGF (670.94 ± 86.92 pg/mL),
VEGF (809.53 ± 95.98 pg/mL), collagen (921.47 ±
49.65 pg/mL), fi bronectin (1466.48 ± 460.21 pg/mL),
and SOD in 5 mL saline solution

This is picogram, the dosages are still pretty low. Regranex a foot ulcer creme carries like 100ug/gram of PDGF-AA, heavily overdosed.

2 years? Damn your positive, to be honest i would expect nothing close in 5 years even. Follica is nowhere with their dermabrasion + lithium, Histogen didn't meet expectations and minoxidil still outperforms it heavily, their ROI isn't probably going worth it. Perhaps replicel, that's it. Furthermore the only thing i see maybe working is this b-catenin small molecule agonist, but I doubt it as there are more negative loops in the WNT pathway.

Seems like this is really the only thing realistically worth it for the coming years. I don't think that it will be better than any anti androgen + minox therapy, but as it works by stimulating various paracrine signalling factors it is a multi-action treatment just like minoxidil and that is what we need.

Did you get any more information from this swiss lab moleular?