Final Days: Chinese Scientists Have Solved the DP Culturing Problem! (2014)

[blmars french]

just one problem ..... : O
it takes 10 years ........................ every 10 years. : (
few hairs on a mouse makes you jump for joy? : Mad:
it's been that mice treated .... : (
(with RU58841 in the 90s)
with CB even pigs have treatment.: (

[Jazz1]

just one problem ..... : O
it takes 10 years ........................ every 10 years. : (
few hairs on a mouse makes you jump for joy? : Mad:
it's been that mice treated .... : (
(with RU58841 in the 90s)
with CB even pigs have treatment.: (

That 10 years is always from western countries lol, let's hope the chinks get this to us quickly through their country.

[blmars french]

should not dream of Asian technology does not precede the West.
should not rejoice too quickly.

[Desmond84]

Firstly, thanks Boldy for getting me the paper Ur a champion. Also, Arashi's explanation is uncanny...couldn't explain it better myself

One interesting point in the study is that these scientists are fully aware of the work of Taiwanese university and have tried to outmatch them! Here’s an excerpt from their paper:

“Previous studies (referenced as the studies conducted by TAIWANESE UNIVERSITY) showed that there are two manners in which DP spheroids form. DP cells were cultured on the surface of plates with low-adhesive capacities or within hydrogels. For the former, DP spheroids with varying diameter can be formed on the first day after seeding through the intercellular collision and fusion, and the size of DP spheroids depends on the seeding density of cells, even for 600μm. However, there is a limited scope for expanding cell numbers. For the latter, DP spheroids also can be formed by cloning growth of DP cells in hydrogels. The size of DP spheroids depends on the time of cell culture, which usually requires about two weeks to form a DP spheroid with a diameter 100μm, hardly exceeding 200μm.

Our result showed that the formation of DP spheroids can be divided into two aspects. In the early stage, DP cells can form spheroids with a diameter of 100μm, through intercellular collision and fusion. In the later stage, the size of DP spheroids mainly increase through cell proliferation within DP spheroids. Generally, the diameter of DP spheroids formed in Matrigel can reach to 250μm, which is similar to normal human scalp DP.”

Very interesting.

Also, I remember another study pointing out the size of DP spheroid having an impact on the hair diameter. The Chinese spheroids were exactly the same size as natrual DP spheroids in our donor zone (250mcg), something that the Taiwanese only achieved in some of the cultured population (at random). So their technique seems pretty solid.

One shortcoming of the paper is that they didn't do a full genetic comparison between the original DP cells and the Cultured. They only tested for the genes known to possess trichogenic potential. But all in all, very solid paper with very promising outcome.

With regards to timelines, we all know how that goes but I personally believe a working cell therapy will be in trials by 2020 the latest and by 2022-2023 we'll have it available to patients at least in Japan. A working cell therapy is defined as converting a NW7 to NW1 with a very high density engineered DHT-resistant hair follicles.

By 2025, you'll have the first Kidney, lung and Liver transplants and further down the line (2028-onwards) nanotechnology will cure hairloss from a new angle, something along the lines of what Molten is getting at.

__________________________________________________ _____________

Also, guys I've been stuck on the calculations for the CB-03-01 formulation. Sorry it's taking a bit longer than I promised. I'm reading up on guidelines about some of the ingredients and what dose range should be in a topical cream. We're looking at over 10 ingredients all serving a specific purpose. I'll have it all posted for you guys by the end of this weekend Sorry once more.

P.S, JCM and other brothers...my Fin micro-dosing is going strong 0.25mg TWICE A WEEK has maintained my hair for well over 20 months now. I'll update my thread when I get the chance in case you didnt see this.

[fred970]

Yeah of course, French technology is the best. France will come up with a cure for hair loss.

[kissmyscalp]

Maybe French technology is not the best but French medicine IS, so shut up.

[Tenma]

Yeah they grew cosmetically correct HUMAN hair on mice, as you can see on the photo's. However they didnt use cultured DP cells (they couldnt culture human DP cells back then), they just used existing DP cells which they took from human donors. Jahoda managed to culture DP cells last year for the first time in history, but they lost gene expression so the resulting hair was not correct like in Tsuji's experiment and it lacked colour and thickness. Anyway that problem should now thus be solved thanks to the Chinese

So, theoretically we're done It just needs to be shown to work now on human skin. Jahoda already did that last year of course (human foreskin transplanted onto mice), but still, we'd need to see the whole process now, resulting in human hair on human skin, grown from human CULTURED DP cells.

I see. We are really close! All we need is to succeed in clinical trials. Its really cool that taiwanese are already planning to start testing 3D spheroid culture of DP cells on humans.

Very exciting times!

[burtandernie]

We really need to see all of this though put together and this hair being grown on humans to really see that it all works as expected. It would also be nice to see a third party verify this all works.
Yeah well the US is going to fall behind other countries because few people go into engineering or sciences because its wrongly viewed as nerdy or boring. Whatever country has more educated people will lead the future

[Hair Bear]

Just today I was looking at my sisters hair and just wanted to run my finger through it as I once was able to do mine, it looked so complete and full.

I'd love to have that confidence and sensation back some day.

[locke999]

Curious question. When was the last time an Asian country came out with some innovated and not copied from the United States. What are some popular products we are using that was created by an Asian country. I know that Japanese cars are used but cars were not something Japan innovated.

[burtandernie]

It would be cool to see someone NW 7 be a NW 0 a week later. Then we could see how big a difference hair really makes and I do think it does make a difference.

[HairBane]

Remember that
1. This is happening in Asia. Who knows how quickly this could be approved over there once stem cell science is a little more understood and mature in say, 6-7 years. China really want to prove themselves as innovators in the science and technology industries, so they'll probably try to get things like this through approval as quickly as possible.

2. We won't go from no solution to a complete solution all of a sudden. There are plenty of interim solutions that could work really well, especially if used together and compounded. So if this takes 10 years to become available, in 5 years time we should have at least a few things on the market that work for regrowth. Maybe one of Follica/Replicel/Histogen obviously, but also perhaps a better anti-androgen, and likely dozens of things that are off the radar right now.

3. Like Arashi says, once the scientific protocol for hair multiplication is out there, there will be doctors in the less regulated parts of the world hacking a solution for the more adventurous norwoods.

[hellouser]

It would be cool to see someone NW 7 be a NW 0 a week later. Then we could see how big a difference hair really makes and I do think it does make a difference.

Won't be a week, thats for sure. You need to take into account the hair growth cycle;



Then you need to take into account how long it takes the cells to turn into fully functioning follicles... and then you need to take into account that hair grows about 1cm per month.

It'll be a bit of a wait period before you see the results... but once you do, you'll be rocking a socially acceptable head of hair and regain your confidence, self esteem and notice your overall happiness in life SKYROCKET. I can't wait for that day.

[Tenma]

Firstly, thanks Boldy for getting me the paper Ur a champion. Also, Arashi's explanation is uncanny...couldn't explain it better myself

One interesting point in the study is that these scientists are fully aware of the work of Taiwanese university and have tried to outmatch them! Here’s an excerpt from their paper:

“Previous studies (referenced as the studies conducted by TAIWANESE UNIVERSITY) showed that there are two manners in which DP spheroids form. DP cells were cultured on the surface of plates with low-adhesive capacities or within hydrogels. For the former, DP spheroids with varying diameter can be formed on the first day after seeding through the intercellular collision and fusion, and the size of DP spheroids depends on the seeding density of cells, even for 600μm. However, there is a limited scope for expanding cell numbers. For the latter, DP spheroids also can be formed by cloning growth of DP cells in hydrogels. The size of DP spheroids depends on the time of cell culture, which usually requires about two weeks to form a DP spheroid with a diameter 100μm, hardly exceeding 200μm.

Our result showed that the formation of DP spheroids can be divided into two aspects. In the early stage, DP cells can form spheroids with a diameter of 100μm, through intercellular collision and fusion. In the later stage, the size of DP spheroids mainly increase through cell proliferation within DP spheroids. Generally, the diameter of DP spheroids formed in Matrigel can reach to 250μm, which is similar to normal human scalp DP.”

Very interesting.

Also, I remember another study pointing out the size of DP spheroid having an impact on the hair diameter. The Chinese spheroids were exactly the same size as natrual DP spheroids in our donor zone (250mcg), something that the Taiwanese only achieved in some of the cultured population (at random). So their technique seems pretty solid.

One shortcoming of the paper is that they didn't do a full genetic comparison between the original DP cells and the Cultured. They only tested for the genes known to possess trichogenic potential. But all in all, very solid paper with very promising outcome.

Thank you for the update Des!

Really good they are aware of what taiwanese are doing. Lets hope the thing you mention about genetic comparison can be addressed in the near future.

My guess is that if cultured DP Cells were the same size as "normal" DP cells, most genetic expression should be retained.

Maybe im wrong, though. far from being a biology scientist here lol

[Buckerine11]

Firstly, thanks Boldy for getting me the paper Ur a champion. Also, Arashi's explanation is uncanny...couldn't explain it better myself

One interesting point in the study is that these scientists are fully aware of the work of Taiwanese university and have tried to outmatch them! Here’s an excerpt from their paper:

“Previous studies (referenced as the studies conducted by TAIWANESE UNIVERSITY) showed that there are two manners in which DP spheroids form. DP cells were cultured on the surface of plates with low-adhesive capacities or within hydrogels. For the former, DP spheroids with varying diameter can be formed on the first day after seeding through the intercellular collision and fusion, and the size of DP spheroids depends on the seeding density of cells, even for 600μm. However, there is a limited scope for expanding cell numbers. For the latter, DP spheroids also can be formed by cloning growth of DP cells in hydrogels. The size of DP spheroids depends on the time of cell culture, which usually requires about two weeks to form a DP spheroid with a diameter 100μm, hardly exceeding 200μm.

Our result showed that the formation of DP spheroids can be divided into two aspects. In the early stage, DP cells can form spheroids with a diameter of 100μm, through intercellular collision and fusion. In the later stage, the size of DP spheroids mainly increase through cell proliferation within DP spheroids. Generally, the diameter of DP spheroids formed in Matrigel can reach to 250μm, which is similar to normal human scalp DP.”

Very interesting.

Also, I remember another study pointing out the size of DP spheroid having an impact on the hair diameter. The Chinese spheroids were exactly the same size as natrual DP spheroids in our donor zone (250mcg), something that the Taiwanese only achieved in some of the cultured population (at random). So their technique seems pretty solid.

One shortcoming of the paper is that they didn't do a full genetic comparison between the original DP cells and the Cultured. They only tested for the genes known to possess trichogenic potential. But all in all, very solid paper with very promising outcome.

With regards to timelines, we all know how that goes but I personally believe a working cell therapy will be in trials by 2020 the latest and by 2022-2023 we'll have it available to patients at least in Japan. A working cell therapy is defined as converting a NW7 to NW1 with a very high density engineered DHT-resistant hair follicles.

By 2025, you'll have the first Kidney, lung and Liver transplants and further down the line (2028-onwards) nanotechnology will cure hairloss from a new angle, something along the lines of what Molten is getting at.

__________________________________________________ _____________

Also, guys I've been stuck on the calculations for the CB-03-01 formulation. Sorry it's taking a bit longer than I promised. I'm reading up on guidelines about some of the ingredients and what dose range should be in a topical cream. We're looking at over 10 ingredients all serving a specific purpose. I'll have it all posted for you guys by the end of this weekend Sorry once more.

P.S, JCM and other brothers...my Fin micro-dosing is going strong 0.25mg TWICE A WEEK has maintained my hair for well over 20 months now. I'll update my thread when I get the chance in case you didnt see this.

2023 at the earliest? That's disheartening. I'll be married with kids by then, and hair loss will be but a fleeting concern. FML