Tsuji-lab (Team Tokyo)

[Desmond84]

What their journal article stated was that 24 hair germs is required to produce around 120 hairs/cm2.

I hope Dr Lauster continues with his work as well

I think both methods are groundbreaking and who knows what else may be going on in other academic labs that we are not aware of yet!

What's important is science is finally catching up to the hairloss community which is amazing

[hellouser]

Yep So what they do is they create a hair seed (hair germ) made up of specific stem cells and inject it in your dermal layer.

In mice, after 22 days the hairs started to grow, connected to all the surrounding structures including nerve cells. They could even induce a goose bump and the hairs would stand up straight LOL

The amazing things is the hair seeds produced 70% of natural hair density a mice normally has which is unheard using our current treatments

Hmm... sounds like Lauster still has the leg up on them as he's created the whole follicle, that 70% figure still kind of sounds like a shot in the dark similar to what aderans/replicel are doing by not knowing how well it work.

With Lauster's work it seems like a patient would walk in and say 'I want 1,500 grafts' and he says 'Done.' and he creates exactly that many. Though with FUE is it even possible to hit 100% natural density? I'd imagine 20,000 grafts to cover 300cm2 of bald scalp would take a toll and probably require multiple visits, no?

How would Tsuji labs know how many cells are injected? Are they all counted? Do all the cells turn into hair for sure?

[Desmond84]

Thanks Desmond!

So Tsuji labs treatment won't require transplant, just injection?

Yep So what they do is they create a hair seed (hair germ) made up of specific stem cells and inject it in your dermal layer.

In mice, after 22 days the hairs started to grow, connected to all the surrounding structures including nerve cells. They could even induce a goose bump and the hairs would stand up straight LOL

The amazing things is the hair seeds produced 70% of natural hair density a mice normally has which is unheard using our current treatments

[hellouser]

I'm not as familiar with his work but from what I understood he took on a much larger task by creating an entire follicle from scratch! This method involved creating multiple cell lines (not just 2 types of stem cells) and will still require a FUE transplantation method!

By the sound of things though (as unreliable as Nigam can be), it seems that Dr Lauster has moved on to other research interests and is no longer involved in hair follicle regeneration! Again, something that Nigam claims from being in contact with him on a regular basis,and NOT from any valid source!

Thanks Desmond!

So Tsuji labs treatment won't require transplant, just injection?

[Desmond84]

Great explanations!

Can you (if you know) explain how Dr. Roland Lauster's work is done and how it compares to Tsuji Labs?

I'm not as familiar with his work but from what I understood he took on a much larger task by creating an entire follicle from scratch! This method involved creating multiple cell lines (not just 2 types of stem cells) and will still require a FUE transplantation method!

By the sound of things though (as unreliable as Nigam can be), it seems that Dr Lauster has moved on to other research interests and is no longer involved in hair follicle regeneration! Again, something that Nigam claims from being in contact with him on a regular basis,and NOT from any valid source!

[Desmond84]

Been googling and researching more, all this stuff is damn interesting So it turns out until now NO clinical trials with iPS cells have been performed, but that's expected to happen within 1-2 year, as you can read in this very interesting interview with Shinya Yamanaka, THE man when it comes to iPS cells http://ajw.asahi.com/article/behind_...AJ201303220009

Oh wow! very nice find

Looks like we'll know the safety of this treatment sooner than many of us thought!

Future looks very promising indeed

[hellouser]

Thx brother

What Tsuji lab is working on is complete formation of brand new follicles!

Replicel & Aderans are working on creating chimeric follicles, which is a mix of your old hair which is DHT-sensitive and cells from your Donor area which is DHT-resistant! How effective this method is should be announced by July this year

So far, Tsuji lab has figured out how to completely engineer a new hair follicle and grow on a hairless mice! What they're stuck on is how to mass produce these hairs! i.e. how to get mass produce all the necessary building blocks to manufacture a hair follicle!

Hope that helps

Great explanations!

Can you (if you know) explain how Dr. Roland Lauster's work is done and how it compares to Tsuji Labs?

[Desmond84]

Hey Desmond,

Never had the chance to congratulate you for all your informative posts, positivity and research so...Thanks lol

I have been more focused on experimental hairloss treatments instead of possible future "cures"! Have to admit that Tsuji Lab's work is quit interesting and promising...

Anyway I'm not sure what their method consist of...Is it any close to Aderans/Replicel method? Like taking a "sample" of cells from the back of the head, select the ones you want multiply, grow them in appropriate medium, inject all over scalp and BOOM full head of hair?

(sorry for the ignorance!)

Thx brother

What Tsuji lab is working on is complete formation of brand new follicles!

Replicel & Aderans are working on creating chimeric follicles, which is a mix of your old hair which is DHT-sensitive and cells from your Donor area which is DHT-resistant! How effective this method is should be announced by July this year

So far, Tsuji lab has figured out how to completely engineer a new hair follicle and grow on a hairless mice! What they're stuck on is how to mass produce these hairs! i.e. how to get mass produce all the necessary building blocks to manufacture a hair follicle!

Hope that helps

[hellouser]

OK, from what I understand, there are THREE types of stem cells:

1) MULTIPOTENT: These cells can only form specific cell types. For example, multipotent stem cells in the brain can only form neural cells and glia! Those in the bone marrow can only give rise to different blood cell types!

2) PLURIPOTENT: These can give rise to almost ANY cell type.

3) TOTIPOTENT: These can form ANY cell and have the potential to create a complete organism!

Now, there are THREE types of stem cells (all are multipotent btw) in your hair follicle:

- Mesenchymal stem cells

- Dermal Papillae

- Melanocyte stem cells (responsible for giving you the specific colour of your hair)

If we are able to turn iPS cells into these THREE types of stem cells then we can mass produce hair germs and implant them into our scalps!

Within 6-9 months we should have a natural hair destiny as this is exactly what happens to an embryo during its development around the third month of pregnancy!

Thank you This is great information!

[Desmond84]

OK, from what I understand, there are THREE types of stem cells:

1) MULTIPOTENT: These cells can only form specific cell types. For example, multipotent stem cells in the brain can only form neural cells and glia! Those in the bone marrow can only give rise to different blood cell types!

2) PLURIPOTENT: These can give rise to almost ANY cell type.

3) TOTIPOTENT: These can form ANY cell and have the potential to create a complete organism!

Now, there are THREE types of stem cells (all are multipotent btw) in your hair follicle:

- Mesenchymal stem cells

- Dermal Papillae

- Melanocyte stem cells (responsible for giving you the specific colour of your hair)

If we are able to turn iPS cells into these THREE types of stem cells then we can mass produce hair germs and implant them into our scalps!

Within 6-9 months we should have a natural hair destiny as this is exactly what happens to an embryo during its development around the third month of pregnancy!

[Arashi]

That first iPS human clinical trial is even expected to begin within a year, according to this article: http://blogs.nature.com/news/2013/02...man-trial.html

[Arashi]

Been googling and researching more, all this stuff is damn interesting So it turns out until now NO clinical trials with iPS cells have been performed, but that's expected to happen within 1-2 year, as you can read in this very interesting interview with Shinya Yamanaka, THE man when it comes to iPS cells http://ajw.asahi.com/article/behind_...AJ201303220009

[Arashi]

Desmond, help me out here, the more I read the more I'm getting confused

If I understand correctly, Team Tokyo ALREADY knows (and successfully demonstrated) how to make a hair follicle out of Mesenchymal & Dermal stem cells. These 2 types of stem cells are 'multipotent', instead of pluripotent (and thus restricted by lineage)

Researchers also already know how to make make pluripotent stem cells (and after today's finding that's even become better and easier). I know that currently the big hurdle in this field of research is that researchers don't know that much yet about the processes that induce a pluripotent stem cell to become a certain differentiated cell type. But obviously Team Tokyo already knows how to make differentiated hair follicle cells out of these MULTIpotent stem cells. So what's the problem here exactly that's holding this back ? Turning pluripotent stem cells into multipotent Mesenchymal & Dermal stem cells ?

[Arashi]

While this of course is an interesting step forward (until now THE problem with IPS has been the risk of cancer), the major hurdle is of course inducing those stem cells to actually form follicles. This process in general is not very well understood yet by scientists (understanding the chemical reactions that induce a stem cell to form tissue).

I think I've not expressed this correctly. The problem is to get stem cells to turn into those epithelial stem cells and dermal papilla cells. The researched already succeeded at creating HUMAN hair follicles from combining those 2 types of stem cells, so the problem here currently is to get general stem cells turn into those subtypes of stem cells. At least, that's what I think.

Am I correct here Desmond ?

[moore]

Thank you so much for these info guys.
2020....looks like a lot of time.