50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

[drnigams]

Boldy,
Your knowledge of the subject fascinates me.
Can you mail me the links regarding 3d culturing studies for hair germ creation.I had discussed 3d culture with Dr Gerd.
We do culture Keratinocyte stemcells present in the bulge of follicle.
Can you elaborate more on the KC cell requirement for hair germ formation.
Regarding PCR screening ,yes we do process the samples in thermocycler and watch cells in GELDOC for any mutation.
You are right any biotec will understand regarding HM cell culturing is nothing big,and the real challenge is ahead to create potent dp culture,dp implant and hair germ invitro creation and regrowth of the same on human scalp.

Good to see someone has the balls to step forward from the already well paid ht solutions, and trying something new that is the cure. (if its in real world as nice as mice/ paper)



@ nigams, arent the germcells created by 3d culture by putting dp and epithelial cells together? this procedure should allow new hair where its never excited before (from nw0 to nv negative for example) I remember a study where they combined epthelial cells + dp cells to create hair on a mice ear.
where DP cells alone couldn't do it since there where no KC cells.
Regarding PCR screening ,yes we do process the samples in thermocycler and watch GELDOC for any mutation.
You are right any biotec will understand regarding HM cell culturing is nothing big,and the real challenge is ahead to create potent dp culture,dp implant and hair germ invitro creation and regrowth of the same on human scalp.


Important to always do PCR screening to check for mutations, although they were not found in some studies even after 90 passages, its still a must.

regarding the procedure you are doing nigams, there is nothing special about it, its just basic cell culture/ biology if you ask me, so I wonder why the **** we had to wait all these years, while this procedure is known for at least 15 years.....

it makes me thinking that drug companies are behind this slow process....

If you bring this forward, even the haters on this forum will love you,

I'm sure

[Boldy]

All sounds very interesting and exciting Dr. Nigam. If your work with Dr. Gerd is successful it will be a huge leap forward and bring to hair loss sufferers a treatment that was not expected for another 10 years. You seem to good to be true Dr. Nigam and that worries me si I won't get my hopes up too much but I think that what you preach is very true - we will beat hair loss by working TOGETHER. By getting in touch with the other doctors who are workung on this problem and trying to work with them you are taking the industry forward hugely. It's ridiculous that it's taken someone so long to do this.

Good to see someone has the balls to step forward from the already well paid ht solutions, and trying something new that is the cure. (if its in real world as nice as mice/ paper)



@ nigams, arent the germcells created by 3d culture by putting dp and epithelial cells together? this procedure should allow new hair where its never excited before (from nw0 to nv negative for example) I remember a study where they combined epthelial cells + dp cells to create hair on a mice ear.
where DP cells alone couldn't do it since there where no KC cells.

Important to always do PCR screening to check for mutations, although they were not found in some studies even after 90 passages, its still a must.

regarding the procedure you are doing nigams, there is nothing special about it, its just basic cell culture/ biology if you ask me, so I wonder why the **** we had to wait all these years, while this procedure is known for at least 15 years.....

it makes me thinking that drug companies are behind this slow process....

If you bring this forward, even the haters on this forum will love you,

I'm sure

[UK Boy]

UKBoy,
Aderans are into autologous stemcell solution injections 6weeks apart.
We already have similar solution with similar results like aderans as on today.But these results need to be improved.
Aderans i think but not sure have recently also started dp culturing,i am not sure is the dp culture part of their clinical trial or not.Some one can find out and help me with that.
According to me the number one guy is the german Dr.Gerd than comes aderans,histogen,replica etc. who has already created hair germ in the lab and published that paper.
Since he is not a surgeon neither they are into clinical trials at present hence the effort has not resulted into commercial availability of hair germ implant.May be most probably i will take their work foraard into clinical trials or if hopefully get a sanction from indian regulators to allow me to use hair germ as part of medical procedure rather than as adrug ,in that case in 2014 we should be able to offer to the patients from our clinic in BOMBAY,India.
Another Scientist i am impressed with and will meet him this year is Takashi Tsuji, a regenerative medicine specialist of Tokyo university of science who created 95% growth on mice after injecting human hair stemcells.
Ofcourse the brilliant UK researcher Jahoda ,whom i am meeting in may2013, he injected his dp cells on his wife's arm and got 2 growing follicles.
Regarding regulations if you want to launch any stemcell product for mass use in the market...the full process of clinical trials has to be completed but if you are offering in a limited way under monitoring of doctors from ones clinic or hospital than in certain counties it is given permission as a medical procedure rather than classified as a drug.
We have the clearance from Independent Institutional committee for stemcell research and therapy with minimum manipulation as medical procedure from our specific clinic at mumbai and also clearance from Independent ethics Committee.
We have also register ourself with the regulatory authorities and informed the details of our process ,
we also need to summit all the records of the patients with followup to the regulatory authorities.
All our materials should be sourced from the FDA registered manufacturers or distributors including imported products .Which we comply with the bills and invoices.
Dr Gerd will help us as a consultant shortly and will also visit the mumbai lab when we finalize our contract next month.


With the help

All sounds very interesting and exciting Dr. Nigam. If your work with Dr. Gerd is successful it will be a huge leap forward and bring to hair loss sufferers a treatment that was not expected for another 10 years. You seem to good to be true Dr. Nigam and that worries me si I won't get my hopes up too much but I think that what you preach is very true - we will beat hair loss by working TOGETHER. By getting in touch with the other doctors who are workung on this problem and trying to work with them you are taking the industry forward hugely. It's ridiculous that it's taken someone so long to do this.

[drnigams]

UKBoy,
Aderans are into autologous stemcell solution injections 6weeks apart.
We already have similar solution with similar results like aderans as on today.But these results need to be improved.
Aderans i think but not sure have recently also started dp culturing,i am not sure is the dp culture part of their clinical trial or not.Some one can find out and help me with that.
According to me the number one guy is the german Dr.Gerd than comes aderans,histogen,replica etc. who has already created hair germ in the lab and published that paper.
Since he is not a surgeon neither they are into clinical trials at present hence the effort has not resulted into commercial availability of hair germ implant.May be most probably i will take their work foraard into clinical trials or if hopefully get a sanction from indian regulators to allow me to use hair germ as part of medical procedure rather than as adrug ,in that case in 2014 we should be able to offer to the patients from our clinic in BOMBAY,India.
Another Scientist i am impressed with and will meet him this year is Takashi Tsuji, a regenerative medicine specialist of Tokyo university of science who created 95% growth on mice after injecting human hair stemcells.
Ofcourse the brilliant UK researcher Jahoda ,whom i am meeting in may2013, he injected his dp cells on his wife's arm and got 2 growing follicles.
Regarding regulations if you want to launch any stemcell product for mass use in the market...the full process of clinical trials has to be completed but if you are offering in a limited way under monitoring of doctors from ones clinic or hospital than in certain counties it is given permission as a medical procedure rather than classified as a drug.
We have the clearance from Independent Institutional committee for stemcell research and therapy with minimum manipulation as medical procedure from our specific clinic at mumbai and also clearance from Independent ethics Committee.
We have also register ourself with the regulatory authorities and informed the details of our process ,
we also need to summit all the records of the patients with followup to the regulatory authorities.
All our materials should be sourced from the FDA registered manufacturers or distributors including imported products .Which we comply with the bills and invoices.
Dr Gerd will help us as a consultant shortly and will also visit the mumbai lab when we finalize our contract next month.


With the help

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

[UK Boy]

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

Also why did you not consider DP culturing years ago when Aderans and Intercytex first mentioned it?

Will you also try culturing Dermal Sheath Cup Cells like Replicel as well or do yoy not think thst those cells will work? Are you allowed to/able to culture DSC cells?

Sorry if you have previously mentioned all this elsewhere. The cell growth side of your work is of more interest to me than all the hair transplant stuff.

[UK Boy]

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

[didi]

Im impressed with regrowth in donor area(around bmark),
wow..90% regrowth ,it beats hst thats for sure...

I can see power shift...from Holland to India

[drnigams]

Didi,I have mailed before (large ) pics of donor to NSN including the before pics of birth mark.
Not just 2 follicle grafts but he may get extra regeneration both at donor and recipient because we injected dp cells,dp,activated stemcells,growth factors and ecm.
BTW it is not 80% ,one graft is not in the view in the photo,90% regeneration is what i am expecting around birth mark and am i waiting for the last extraction and would like to watch it some more time.
Some times due to human error technician may end up extracting the whole graft.You can see the healing which is clean and very quick.
Theoretically donor regeneration has to be 100%,practically due to human error it may range between 90 to 95% donor regeneration.
More imp is to watch the recipient regeneration,because it contains the bisected graft without the dermal papilla and blood supply.

You sayiing 80% regrowth,
we need to know what was extracted(type of grafts) and compare it to what is growing back(do 3 hair grafts grow as 3 hair grafts)

also do you know where you planted those grafts in recipient?

its good to see you are about to crack Ghos technique,


can you upload the pictures on TBT forum please, HS is pain to navigate[/QUOTE]

[didi]

Yes, I have seen the pic on HS,

You sayiing 80% regrowth,
we need to know what was extracted(type of grafts) and compare it to what is growing back(do 3 hair grafts grow as 3 hair grafts)

also do you know where you planted those grafts in recipient?

its good to see you are about to crack Ghos technique,


can you upload the pictures on TBT forum please, HS is pain to navigate

[drnigams]

Gc,Didi,
did you have a look at birthmark pics of nsn showing 8 extraction regrowth out of 9 visible.Also gc kindly mail me or post your mail id so that i can mail you large pics,you had posted your mail id before,i am not getting that post.

[drnigams]

UK Boy,
Dr Gerd will be joining our team as a biotech consultant in april.He is the one who created world's microhair follicle or hair germ in the world including dermal papilla for implantation.First we will do mini trial with permission from authorities.If it shows hair regeneration we will go for world's first such clinical trial in india,for hair germ or microfollicle implant.The mini trial at the clinic as medical procedure should start by nov/dec.2013.And we will know early results in 3 to 6 months of injections of microfollicle and than the clinical trials will start.I am also in touch with indian authorities as new bill on clinical research is about to be presented in the indian parliament by this year end.
Till that time the next thing is finishing our dp culture in next 2 months and moving towards dp implant development .
Till that time i am utilising dp cells and body dermal papilla with my autologous stemcell solution.,Which is equally good with some limitation on number of follicle developed on a bald scalp.We will be meeting the german team in scotland hair research conference on may 4th,5th and 6th 2013.
If i get time, may fly to their lab at berlin,Their main focus is no more hair follicle creation as they have already created and now it is for people like us to do trials and get into the market place.They are now into development of human physiology on a microchip which cam be used for drug testing and speed up molecular development for new drugs.Which is an ambitious project.

Dear Dr. Nigam

Thank you for all your posts and updates but I but just reading through a thread on Hairsite and I noticed that your were supposed to be visiting the German scientists in Berlin again in March. We're now entering into April and I did not see you mention this trip at all, in fact with all the posts you've been making I do not see that you would have had time

So did you visit Berlin or not? and if not do you intend to go soon? If you did visit in March what came out of the visit? How is their research coming along? Do they intend to start clinical studies soon?

Apologies if you did indeed mention the visit somewhere, all I've seen is information about are your case studies.

IThanks in advance for your answer.

[UK Boy]

Dr. Nigam trip to Berlin in March??

Dear Dr. Nigam

Thank you for all your posts and updates but I but just reading through a thread on Hairsite and I noticed that your were supposed to be visiting the German scientists in Berlin again in March. We're now entering into April and I did not see you mention this trip at all, in fact with all the posts you've been making I do not see that you would have had time

So did you visit Berlin or not? and if not do you intend to go soon? If you did visit in March what came out of the visit? How is their research coming along? Do they intend to start clinical studies soon?

Apologies if you did indeed mention the visit somewhere, all I've seen is information about are your case studies.

IThanks in advance for your answer.

[534623]

Iron man

since you are so knowledgable on the subject of hair loss why dont you offer to work for dr nigam,be his chief advisor for europe?

That is easy to explain:

I'm incorruptible on one hand, unaffordable on the other hand.

What part of this don't you understand?

I mean, not even the NASA has been able to get me...

[didi]

Iron man

since you are so knowledgable on the subject of hair loss why dont you offer to work for dr nigam,be his chief advisor for europe?
maybe you can speed up the process and bring the cure earlier

[534623]

hmmm, maybe, but I don't think that answering of at least pkt 1) and 2) will be that long. So please answer at least pkt. 1) and 2).

hmmm, seems you need some help ...

So can you please explain me a few things:

1) From which study did you use this scientific pic (red questionmarks)?
2) What's the author's name?
3) What's the graphic all about? What exactly does it explain?
4) In which context is this pic at all with all the other "doubling" procedure photos you posted in this HS thread (see the snippet of this post above)?

The author's name is Dr. S. Millar and published the study (including the pic you used) in 2002.
So what's the papers TITLE?
...and so on and so on.

I mean, seriously:
You post unlabeled scientific pics on your website and hair loss forums, and it seems that you don't even know anything about the pics.