First Pics of 3D spheroidal DP aggregate @ Dr. Nigam's Lab

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  • veca
    Senior Member
    • Jan 2012
    • 219

    #16
    [B]This is similar to 2d dp culture,except they are now aggregated in 3d spheroids to improve trichogenicity. The case study with 3d spheroid dp culture will start tmr.

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    • brunobald
      Senior Member
      • Jul 2013
      • 169

      #17
      Veca, are you injecting these into a completly bald spot? Is there any other proto call other than the injection itself. Thanks

      Comment

      • UK_
        Senior Member
        • Feb 2011
        • 2691

        #18
        This looks interesting.

        Comment

        • UK_
          Senior Member
          • Feb 2011
          • 2691

          #19
          Originally posted by hellouser
          I'll be singing koombaya with a ukelele in one hand and an entire bottle of everclear in the other for the purpose of DRINKING!
          I'll be dancing around my roof with a keytar and a bottle of gin .

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          • clandestine
            Senior Member
            • Aug 2011
            • 2002

            #20
            Yes, we'll all be right drunk. For now let's wait on this and see; veca says it starts tomorrow.

            Comment

            • brunobald
              Senior Member
              • Jul 2013
              • 169

              #21
              How long did it take the injections to grow hairs on the foreskin grafted to the mouse?

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              • UK_
                Senior Member
                • Feb 2011
                • 2691

                #22
                When can we all start booking to have this procedure done?

                Also, I hope this is true DP cell technology and not just useless PRP.

                Comment

                • Arashi
                  Senior Member
                  • Aug 2012
                  • 3888

                  #23
                  Originally posted by brunobald
                  How long did it take the injections to grow hairs on the foreskin grafted to the mouse?
                  6 weeks

                  Comment

                  • Arashi
                    Senior Member
                    • Aug 2012
                    • 3888

                    #24
                    Originally posted by hellouser
                    With all this information of culturing cells and injecting, multiplying them, etc. Why hasn't anyone attempted to do any of this kind of work on their own apart from Dr. Nigam?

                    How difficult is it to culture these cells and get a working treatment while give the one finger salute to everyone and especially the FDA? Does ANYONE know what kind of equipment one would need?
                    Yeah I wish somebody else was doing this experiment. Cause we all know, in a few months Nigams will present some horrible comparison pictures, blurry, different lighting, different angle, different hair length, low resolution from a patient who's on all the meds there are, most probably is using concealer and we'll still know nothing.

                    Comment

                    • Arashi
                      Senior Member
                      • Aug 2012
                      • 3888

                      #25
                      Also keep in mind that Jahoda et al only partially restored the trichogenic potential. It's currently still unknown why they couldn't fully restore it. Not partypooping here, but just saying we should have realistic expectations.

                      Anyway, it's interesting for sure and it's a good thing that Nigams is doing this experiment, that's for sure. But I'm afraid that if he fails to replicate wat Jahoda did, or even get some partial success, he'll use all the trickery in the book again to bloat results. I wish this guy was just doing honest business.

                      Comment

                      • brunobald
                        Senior Member
                        • Jul 2013
                        • 169

                        #26
                        Originally posted by Arashi
                        6 weeks
                        Is that six weeks to break the surface and become visable or six weeks to show the first signs of forming a follicule under the skin. Nigam was showing of a special ultrasound specificly for detecting follicules for his doubling process maybe he could use it to look for the beginings of hair follicule formation.

                        The hanging droop method for creating 3d spheriods looks quite simple.

                        Comment

                        • Arashi
                          Senior Member
                          • Aug 2012
                          • 3888

                          #27
                          Originally posted by brunobald
                          Is that six weeks to break the surface and become visable or six weeks to show the first signs of forming a follicule under the skin. Nigam was showing of a special ultrasound specificly for detecting follicules for his doubling process maybe he could use it to look for the beginings of hair follicule formation.

                          The hanging droop method for creating 3d spheriods looks quite simple.

                          https://www.youtube.com/watch?v=E98sKuJZkBw
                          Jahoda et al observed marked follicle neogenesis by 6 weeks, that's all I know. Would love to read the whole study, if anybody can find it, please do post it !!

                          Comment

                          • Arashi
                            Senior Member
                            • Aug 2012
                            • 3888

                            #28
                            Originally posted by veca
                            [B]This is similar to 2d dp culture,except they are now aggregated in 3d spheroids to improve trichogenicity. The case study with 3d spheroid dp culture will start tmr.
                            According to Jahoda, DP cells always lose their inductive capability when cultured in the old way. Nigams always claimed success, but the one case we could actually verify, OBI's case, failed and all we saw were horrible comparison pictures from other cases. This is pretty much proof that Nigams was lying, am I right or am I missing something ?

                            Thing is, I'm afraid he'll do that again. If he wants to convince us, make sure he'll finally start posting GOOD photo's, instead of ones in which he uses all the trickery in the book to simulate result.

                            Comment

                            • hellouser
                              Senior Member
                              • May 2012
                              • 4419

                              #29
                              Originally posted by Arashi
                              According to Jahoda, DP cells always lose their inductive capability when cultured in the old way. Nigams always claimed success, but the one case we could actually verify, OBI's case, failed and all we saw were horrible comparison pictures from other cases. This is pretty much proof that Nigams was lying, am I right or am I missing something ?

                              Thing is, I'm afraid he'll do that again. If he wants to convince us, make sure he'll finally start posting GOOD photo's, instead of ones in which he uses all the trickery in the book to simulate result.
                              We're better off pitching in as much cash is needed, buying equipment and doing this shit on our own, get Desmond to do all the work and we're set.

                              Comment

                              • UK_
                                Senior Member
                                • Feb 2011
                                • 2691

                                #30
                                Originally posted by hellouser
                                We're better off pitching in as much cash is needed, buying equipment and doing this shit on our own, get Desmond to do all the work and we're set.
                                I'd contribute.

                                Comment

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