First Pics of 3D spheroidal DP aggregate @ Dr. Nigam's Lab

[brunobald]

The spheroidal DP seem happy on there own sitting in the dish. I take it they are still alive so to speak. I wonder what is the first step they take to becoming a hair follicule. I wonder if outside of the body you can provoke them into doing something under the micro scope. What if you added some known scalp native chemcials, proteins, blood whatever into the petri dish and observe their reaction. If you setup a timelapse camera you could see things happening over a longer time. This is something we could do ourselves. If something provoked a responce you could start removing things from the ****tail and start mapping out the steps needed for neogenisis.

[veca]

I'd definetely chip in $1000. Though I'm afraid it's a bit of an unrealistic idea. We'd need someone with expertise, to operate in a good lab in a non western country and people to experiment on. I don't think any of these are easy to get.

you're really crazy, I thought you were just a pessimist, but I'm pretty sure you're crazy, and I hope you're kidding. :-)

[Arashi]

you're really crazy, I thought you were just a pessimist, but I'm pretty sure you're crazy, and I hope you're kidding. :-)

Care to explain that statement ? Or are you just angry ? And I'm not a pessimist by far. I just know a con artist when I see one. Well maybe a con artist isn't the right word. A con artist wouldn't go as far with these experiments. But he definitely is misleading people with photoshopped images and lies, trying to sell them a worthless service, just to get money out of their pockets. And I'm allergic to that. That's all.

[didi]

What is significance of these photos posted by dr Nigam?

Does it mean he can give us unlimited number of grafts or is it just another session of mental masturbation

[Arashi]

What is significance of these photos posted by dr Nigam?

Does it mean he can give us unlimited number of grafts or is it just another session of mental masturbation

They're pretty useless indeed. I'd rather hear how he thinks he's going to convince us. Is he going to inject Boldy for example soon ? And if so, is he going to do it on a slick bald part, like just below the hairline ? That would be pretty exiting. But if he's going to do his experiment on some random Indian guy with diffuse thinning and show us AGAIN some blurry before/after pictures with different lighting, angle, hair length and it's impossible to verify the validity of the photo's then ... who cares. Just another episode in the Nigams book of lies and deception.

[mari0s]

They're pretty useless indeed. I'd rather hear how he thinks he's going to convince us.

Could just please, please stop doing this? You have made your point. It will take months to see first results do you want pass all that time repeating every message that he is a cheater that use every trickery in the book ecc ecc? You are becoming irritating just like ironman. When and if he will post blurry photo then you can complain how much you want (we will do toghether) but for once it would be nice to have a thread of Nigam's procedure without dozen and dozen pages of insults and whining.

[veca]

Could just please, please stop doing this? You have made your point. It will take months to see first results do you want pass all that time repeating every message that he is a cheater that use every trickery in the book ecc ecc? You are becoming irritating just like ironman. When and if he will post blurry photo then you can complain how much you want (we will do toghether) but for once it would be nice to have a thread of Nigam's procedure without dozen and dozen pages of insults and whining.

I thought I was the only one who thinks so. Thank you brother

[didi]

No seriously guys, Im not following every Nigams case(especially his HM technique, its still many years away)

can somebody explain to me in plain English why should we be excited with these photos of 3D spheroidal thing,

Is it like major discovery or?

[jay woo]

Tomorrow, we will inject 3D spheroids on the first patient and wait to see the outcome and will keep you all updated.
Respected Jahoda says in published study that, we should expect a new hair follicle/s from these injected 3D spheroidal DP cultured cells. Ofcourse i am waiting also report of versican and alkaline phosphate marker report which will conform trichogenic potential of these 3D spheroids.

Has the versican and alkaline phosphate marker report arrived? Is it possible to see the report?

I am still amazed at peoples opinions on researchers. I do not think they are hiding anything. Most of them have a huge ego to feed. They would like nothing more than to be able to go look at what I have done. Research always takes longer than expected. Research is mostly about incremental improvements. Eventually you pass the goal, you just do not know how far the goal is until you reach it.

Hellouser buying equipment and reading every journal on a subject doesn't guarantee success. It is so much harder to succeed and remember failure is always part of the path to success. If you buy a computer and software and read every book on programming are you now a programmer (quality programmer)?

[Arashi]

Could just please, please stop doing this? You have made your point. It will take months to see first results do you want pass all that time repeating every message that he is a cheater that use every trickery in the book ecc ecc? You are becoming irritating just like ironman. When and if he will post blurry photo then you can complain how much you want (we will do toghether) but for once it would be nice to have a thread of Nigam's procedure without dozen and dozen pages of insults and whining.

I was just responding to Didi's remark. He made a very valid point in my opinion. If you disagree, then please focus on the discussion and explain to Didi and me what's the use of these photo's. Thanks.

[Arashi]

Hellouser buying equipment and reading every journal on a subject doesn't guarantee success. It is so much harder to succeed and remember failure is always part of the path to success. If you buy a computer and software and read every book on programming are you now a programmer (quality programmer)?

Hehe. Well I think your example is pretty bad cause yes, you actually can be a quality programmer if you have some brains and just read some books, right from the start. In fact, one of the best programmers I know started programming some really complex software, right after just reading some books. While I know others with years of experience who suck hairy balls. Programming actually is much more about knowing and understanding theory than about experience.

Anyway, Hellouser merely meant that we'd need to find somebody with expertise. Like I said, it's going to be very hard to find someone like that and even harder are the rest of the requirements.

[jay woo]

My point is that the equipment that is required would not be impossible to obtain. It may not be easy to obtain if you lack money and credibility in the industry. For some of the equipment and supplies I would not be surprised that you would require some form of registration and approval. People with in the industry would know the suppliers and the people to talk to. The hard part to find is the people with enough knowledge to be able to come up with new ideas that are safe and plausible.

The ability to use the equipment and obtain meaningful results is difficult. They do not provide an idiots guide to pushing the boundaries in stem cell research. Just like you fiend who is a quality programmer it is about a mindset not just the equipment. The books gave him a leg up but his mindset carried him.

This experiment seems to be about replicating another experiment by another researcher except for using a human as the guinea pig. It will be interesting to see what results are obtained and what lessons are learnt. From the results it will then be even more interesting to see what direction the experiments go, will it be another experiment replication or a new idea from Dr Nigam.

[Arashi]

My point is that the equipment that is required would not be impossible to obtain. It may not be easy to obtain if you lack money and credibility in the industry. For some of the equipment and supplies I would not be surprised that you would require some form of registration and approval. People with in the industry would know the suppliers and the people to talk to. The hard part to find is the people with enough knowledge to be able to come up with new ideas that are safe and plausible.

The ability to use the equipment and obtain meaningful results is difficult. They do not provide an idiots guide to pushing the boundaries in stem cell research. Just like you fiend who is a quality programmer it is about a mindset not just the equipment. The books gave him a leg up but his mindset carried him.

This experiment seems to be about replicating another experiment by another researcher except for using a human as the guinea pig. It will be interesting to see what results are obtained and what lessons are learnt. From the results it will then be even more interesting to see what direction the experiments go, will it be another experiment replication or a new idea from Dr Nigam.

I agree. That's why we're stuck with Nigams.

[mari0s]

I was just responding to Didi's remark. He made a very valid point in my opinion. If you disagree, then please focus on the discussion and explain to Didi and me what's the use of these photo's. Thanks.

Not just that, you have made every post in this thread complaining about Nigam, whats's the point with that? Just to provoke like ironman? I tell you this because i think you are a nice persone just frustrated of lack of clear result, if i would think you are a troll i won't be bother answer to you.

Everytime i open the thread i hope to find some update about markers show thricogenity or not, instead i have to face page and page of complaining, it's quite frustrating

The photos aren't useless, without them someone surely would have questioned if he really use 3D DP cells instead of fresh water.

To put into practice Jahoda work on human, a western company need at list 10+ years (assuming they start today). That photos proves that Nigam did it in 3 months. If he will not obtain good results he can still tring to change protocols or approach without wasting years and years (Replicel is still on DSC cells because using DP or 3D culture would require to start new trials for scratch and another 10 years)

[UK_]

Look, if Nigam can show regeneration with this technique and it is guaranteed not some scam PRP injection instead then I will be booking to go and see him to have thinning areas treated.

Can anyone give a timeframe of when we should know whether this procedure will work or not?