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No need to thank me."massive twat" or "massive bell end" ?
I mean, the change of your train of thoughts from "twat" to "bell end" - what the hell is wrong with you ...
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So - what?
"massive twat" or "massive bell end" ?
I mean, the change of your train of thoughts from "twat" to "bell end" - what the hell is wrong with you ...
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I literally have no idea what you are talking about...btw is that you narrating that video? If so, I am very sympathetic towards you because you don't sound right in the head. You also sound much older, mid 40's maybe? That rings alarm bells right there, a mid 40 year old who loves to argue with people all day long on the internet...nice.Comment
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Smurf,
Till now we were using DP and DS isolated stemcells apart from epithelial cd200 ,cd34, k19,ORS stemcells as a part of our stemcell solution.We realised the below mentioned two mistakes just recently...
1)To wait for at least 1 1/2 month to two months to have sufficient dose of stemcells,and increase the number of injections to 4.
2)After i found out the specific markers for DP cells ,we realized we need sufficient quantity of inducible DP cells.
At present from the next week after speaking to german team i will discuss my new DP and DS cell culture and will start its trial with few samples in the lab and probably by next month should be able to add DP/DC cultured cells with Epithelial stem cells.This should improve results significantly.
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Dr. Nigam's 1st case study pics with Hair Doubling
Dear Friends/Critics,
Hair Doubling of 6 single hair follicle into 12 new single hair follicle at the recipient (1 recipient site showing 2 follicles in a single FU). This is the first case study, 9 more case studies will be conducted for the coming 2 months. This will be an important breakthrough as there are approximately 1300 dermal papilla cells and additional dermal sheath cells per follicle which may allow us hair doubling in multiples with only few follicular units. In both bisected follicles, we have also injected pro-genitor cd200, cd34, ck19, versican and alkaline phosphate positive, epidermal and dermal stem cell respectively. I will explore the legality aspect of this technique in the western world as this technique can be replicated partially even without isolating and activating stem cells. I wish to patent this technique as proto hair organoids and will offer the process training to the fellow doctors with the license fee and continuous up-gradation on the process. With either German OR Japanese team shortly, Dr. Nigam's brand would have better credibility value as experienced and senior bio-technologists and tissue engineers become a part of the brand with their rapport in published scientific world and state-of-art labs. As you are aware of Dr.Nigam's brand stands for applied research and state-of-art, chain of hair transplant clinics which offers access to clinical new procedural development as unpublished data before final procedures are selected and starts undergoing specific clinical trials.
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According to this, you do NOT apply/inject AUTOLOGOUS cells/stuff into the bisected follicles.
So, from which SOURCE do you get the ALLOGENEIC cells?
Derived from a cow? A horse? A dog? Human cadavars?Comment
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534623,
Few grafts were extracted from donor scalp for isolating various epithelial and mesenchymal stemcells,which were injected into the 2 bisected single follicles ,all the 12 bisected follicles were implanted into the reciepient left temple.Where did you get the idea of allogenic stemcells from my post..?
Also both the bisected follicles had some DP cells as they were dissected just below the line of auber.In the 3rd case study we will add cultured inducible DP/DC cells into both the bisected follicles.We expect more than double the growth with addition of potent DP/DC cells.Comment
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Because you didn't mention in your previous post what you mentioned NOW.
Anyway, is the content of this page ...
... the content (including the photos) of this published paper?
If YES, simply say "Yes!". If NO, simply say "No!"
I mean, you can publish on your website whatever you want - that's not the point and not my question. Everything I want to know is whether or not this page contains the whole content (including the photos) of the mentioned published paper by Rossi/Toscani, because I'm not aware of the paper's content and I do not buy such a (toilet)paper if the content of the paper is exactly the same content as on your website. That's the point. So YES or NO?Comment
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Yes ,and i have mentioned his name,not mine.I will put many published papers in public domain on my site as reference on the subject matter of HAIR STEMCELL RESEARCH .I am an applied clinical researcher not a hardcore research development guy and also a practicing hairtransplant surgeon. And remember who will post without patents all the details of its own research,haven't you noticed i have posted much more than my site on the forums,why keep visiting my site when i am live with you..
including the photos) of this published paper?
If YES, simply say "Yes!". If NO, simply say "No!"
I mean, you can publish on your website whatever you want - that's not the point and not my question. Everything I want to know is whether or not this page contains the whole content (including the photos) of the mentioned published paper by Rossi/Toscani, because I'm not aware of the paper's content and I do not buy such a (toilet)paper if the content of the paper is exactly the same content as on your website. That's the point. So YES or NO?[/QUOTE]Comment
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Okay. thanks. But where did you mention the author's names?
Maybe I'm blind, but I can't find the names of the authors.
Everything I can see ...
... is the title of the paper: "Follicular Bisection in Hair Restoration: In Vivo Experiment" - but not the names of the authors:
Rossi A, Gilio M, Pistola G, Carlesimo M, Borroni RG, Fortuna MC, Toscani M.
Furthermore, you didn't publish the REFERENCES they used and mentioned in the paper (around 4 source/references).Comment
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Do you remember you have mentioned about this paper few days back,i know the next page was not opening,i will check tmr and revert..OK .
I have virtually all the important papers like any other researcher on Follicular bisectioning,DP/DC culture,molecular biologyof androgenitic alopecia,Epithelial and mesenchymal stemcells,Tissue engineering of hair follicle and much more .Every researcher or applied clinical researcher always takes advantage of previous work done by other respected authors and takes it forward which are in public domain or buys it from them.
We clinical guys apply this research to bring it to the clinic level for the patients and pharma guys get this research to the pharmacy. It's a team work,everyone has it's own role.Comment
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@ noname,
Isn't it better to let the dr spend his time on research,instead of keep him occupied with questions that can be easily found if you put a little effort into research....
@Nigams, I'm very curious what you are going to do with you DP culture process.. the laste papers regarding dp culture are really hopeful, with good yield... If you can mange to make 40.000 or 80.000 cells from 200 dp's that would be...
Okay. thanks. But where did you mention the author's names?
Maybe I'm blind, but I can't find the names of the authors.
Everything I can see ...
... is the title of the paper: "Follicular Bisection in Hair Restoration: In Vivo Experiment" - but not the names of the authors:
Rossi A, Gilio M, Pistola G, Carlesimo M, Borroni RG, Fortuna MC, Toscani M.
Furthermore, you didn't publish the REFERENCES they used and mentioned in the paper (around 4 source/references).Comment
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BOLDY ,no doubt the last papers were very useful,infact i did not sleep the whole night and straight away went to the lab in the morning to discuss with my biotech,we are in the process of ordering certain media like chang media,keratinocyte conditioning media ,VD3 etc.Today we we did micro dissection of DP from follicle and collected the DP cells with the pipette and counted them and looked for contamination with other epithelial or dermal cells.
The delay is only with the receiving of the supplies mentioned above.We have offices of three international companies in INDIA...,SIGMA ALDRICH,INVITROGEN and CYANNE.
In the mean time i will introduce extracted but not cultured DP cells in the bisected follicles to increase the yield of the follicle.@ noname,
Isn't it better to let the dr spend his time on research,instead of keep him occupied with questions that can be easily found if you put a little effort into research....
@Nigams, I'm very curious what you are going to do with you DP culture process.. the laste papers regarding dp culture are really hopeful, with good yield... If you can mange to make 40.000 or 80.000 cells from 200 dp's that would be...Comment
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Need I remind people that this guy faked photographs for his website? And that he claims to offer pretty much every treatment ever devised, including synthetic transplants, which are banned in the US for very good reasons?
This is not someone you should be trusting! He is a liar and a quack, and does not deserve your attention. I get that you're all a bit desperate, but seriously. There are real treatments on the way. Focus on those.Comment
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This is true. In fact we are waiting that Nigam does an interview with a serious person like Spencer to understand more.
Extra Nigam you said, in another forum, this:
"I think NW2 to NW7 is possible in 2013 itself"
Can you tell us, please, how much money and how much time for this?Comment
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