adipose-derived stem cell protein extract

[nameless]

i believe it is AAPE. which page on theracell did you get that.

It's my understanding that AAPE would have to be stored in certain ways at certain temps and stuff like that. I don't think it can sit on a shelf at a pharmacy and sold like that. I think that would damage it's efficacy.

[zeos]

i believe it is AAPE. which page on theracell did you get that.

not on theracell but related to it,i found it on a medical site,i have already posted it here(go to the end):

https://www.baldtruthtalk.com/showthread.php?t=16196&page=39

so it's important that some of you guys with knowledge of the issue contact them even better call them directly

[nameless]

it's translated from greek to english(from a medical site) but i think it's understandable
what are your thoughts?


Hair Loss Treatment with stem cells

A new medical technique for hair regeneration using stem cells solves in men and women with thin hair and thinning. Treatment is based on the isolation of a large number of proteins, which are released from the stem cells of fat. Innovative therapy has been developed and applied atin Greece in collaboration with the Centre for Plastic Surgery "Cosmetic Restoration" and pioneer biotechnology company regenerative «Theracell». The isolation of stem cells from fat is a revolutionary art, with impressive results and new perspectives in aesthetic medicine. Recent clinical studies show that the regenerative properties of stem cell derivatives have applications in aesthetic medicine and more specifically to regenerate skin and hair regeneration. The skilled and innovative way of making it rich in growth factors 'extract' activates the regeneration and growth of hair, without trichothylakeion and stem cell transplantation.
It is scientifically proven that up to 80% of the action of the stem due to the secretion of a large number of biomolecules, such as growth factors, primarily, cytokines and various enzymes. Very specifically, the fat stem cells secrete more than 700 such proteins, which play an important role in tissue remodeling and regeneration. These proteins act at the site of application:
• Enabling other skin cells (fibroblasts)
• Motivating other cells to migrate to the site of injury
• At the same time protect skin cells from oxidative stress
The treatment process comprises four steps:
1 Collection of a very small amount of fat,
2 Transfer the fat in the laboratory under specific conditions
3 Activation of stem cells,
4 Topical application of treatment in four monthly sessions
Derivatives of activated stem cells after their application to the scalp, promote the growth of hair follicles. Also activate the stem cells already present in the region, so that they start producing new hair and improve existing ones. This technique provides a good, and possibly unique, reliable, scientific alternative for patients who either do not want or are not suitable for hair transplantation. Particularly good results are shown in fine and short hair, which indicates to lengthen and thickens. The application of the new technique is easy, with local anesthesia by skilled, well-educated on the subject physician.

Source: www.ygeia360.gr

It sounds like it could be the correct treatment but I'm not sure if they are using the same growth factors and proteins that are in AAPE. I think that the best thing for you to do would be if you could contact them and ask them to compare what they are doing to what is being done in the AAPE study. The address to the AAPE study can be copied and pasted to the email you send them. You can get the email address to the AAPE study in the first post of this very thread. But I will include it here to save you the trouble of having to look for it. Here it is:

http://www.balancehairrestoration.com/wp-content/uploads/using-proteins-secreted-by-adipose-derived-stem-cells.pdf

[nameless]

not on theracell but related to it,i found it on a medical site,i have already posted it here(go to the end):

https://www.baldtruthtalk.com/showthread.php?t=16196&page=39

so it's important that some of you guys with knowledge of the issue contact them even better call them directly

I don't know Greek. I don't understand them and they wouldn't understand me. I did follow your link to your other post and I did respond to it. Here is what I posted to you:

It sounds like it could be the correct treatment but I'm not sure if they are using the same growth factors and proteins that are in AAPE. I think that the best thing for you to do would be if you could email them and ask them to compare what they are doing to what is being done in the AAPE study. The address to the AAPE study can be copied and pasted to the email you send them. You can get the email address to the AAPE study in the first post of this very thread. But I will include it here to save you the trouble of having to look for it. Here it is:

http://www.balancehairrestoration.com/wp-content/uploads/using-proteins-secreted-by-adipose-derived-stem-cells.pdf

I am preparing an email to send to them but I don't think they will understand what I'm saying to them and I won't understand their reply either if they do respond to me.

[nameless]

not on theracell but related to it,i found it on a medical site,i have already posted it here(go to the end):

https://www.baldtruthtalk.com/showthread.php?t=16196&page=39

so it's important that some of you guys with knowledge of the issue contact them even better call them directly

I went to the source you provided and it looks like a newspaper. I can't understand what the newspaper says so I can't navigate to the article about the specific clinic you're referring to and I have no idea what the name of the clinic is or how to contact them. You did not provide a link to the clinic or their contact information.

[nameless]

The only papers we should be taking with 100% certainty are from Dr. Lauster, Jahoda, Cotsarelis, Christiano and Tsuji.

Here's a proven therapy for you hellouser:

http://www.medicaldaily.com/fda-approves-igrow-low-level-laser-therapy-device-stimulates-hair-growth-men-304890

[zeos]

I don't know Greek. I don't understand them and they wouldn't understand me.....

I am preparing an email to send to them but I don't think they will understand what I'm saying to them and I won't understand their reply either if they do respond to me.

don't think so nameless,you will not have any problems because as you see the whole site(http://theracell.eu/) is only in english,it's more an english speaking enterprise

i've also found the email address of the clinic,
here can you contact them(also in english):

http://www.athanasioschristopoulos.com/english/contact.php

you should contact them(send them an email or give them a call,I prefer the second)because you have the knowledge of the issue

here is the site where i got the information:
http://www.vitamelia.gr/510/therapei...-blastokuttara (in greek)

https://translate.google.de/translat...ara&edit-text= (tranlated in english)

send these 2 sites also to them so that they can understand what are you talking about

good luck!!!

[nameless]

don't think so nameless,you will not have any problems because as you see the whole site(http://theracell.eu/) is only in english,it's more an english speaking enterprise

i've also found the email address of the clinic,
here can you contact them(also in english):

http://www.athanasioschristopoulos.com/english/contact.php

you should contact them(send them an email or give them a call,I prefer the second)because you have the knowledge of the issue

here is the site where i got the information:
http://www.vitamelia.gr/510/therapei...-blastokuttara (in greek)

https://translate.google.de/translat...ara&edit-text= (tranlated in english)

send these 2 sites also to them so that they can understand what are you talking about

good luck!!!

Thanks Zeos. Really, thanks a lot. I'm thankful that someone like you with some gumption and spirit has stepped up to collaborate with me to try to figure out a way way to get these clinics interested in AAPE. Please don't stop thinking of ideas of how I can get clinics interested in AAPE. I will try any good ideas you come up with to get this ball rolling.

I work until 4:30 pm today and after I get off work I'm going to sit down and organize an email for the clinic that you gave me the contact information for. I will include the AAPE study and I will ask ALL of the pertinent questions.

I'll also start looking into how I could possibly contact them by telephone. I have to have my phone carrier give me an international calling plan. But I do prefer emails because these scientific issues are very complex and email text allows me to collect my thoughts. It would definitely be best to lay the communication ground work via email so I can lay out a general idea of what we are asking them to do.

[nameless]

don't think so nameless,you will not have any problems because as you see the whole site(http://theracell.eu/) is only in english,it's more an english speaking enterprise

i've also found the email address of the clinic,
here can you contact them(also in english):

http://www.athanasioschristopoulos.com/english/contact.php

you should contact them(send them an email or give them a call,I prefer the second)because you have the knowledge of the issue

here is the site where i got the information:
http://www.vitamelia.gr/510/therapei...-blastokuttara (in greek)

https://translate.google.de/translat...ara&edit-text= (tranlated in english)

send these 2 sites also to them so that they can understand what are you talking about

good luck!!!

I did email them at all of the email addresses you gave me.

I do not know if email from one person will be enough to persuade them to try AAPE. I wish we could get everyone here at this site to email them too but of course most of these folks lack the gumption to take a positive pro-active step forward. It's easier for them to cry and PMS rant about treatments not being available than to actually do something that might cause a treatment to become available.

Our only realistic short term revolutionary treatment possibilities:

1. AAPE

2. Adipose derived stem cells. But this method may not work because the cells may migrate out of the scalp too quickly. So AAPE is the best bet.

[joachim]

I did email them at all of the email addresses you gave me.

I do not know if email from one person will be enough to persuade them to try AAPE. I wish we could get everyone here at this site to email them too but of course most of these folks lack the gumption to take a positive pro-active step forward. It's easier for them to cry and PMS rant about treatments not being available than to actually do something that might cause a treatment to become available.

Our only realistic short term revolutionary treatment possibilities:

1. AAPE

2. Adipose derived stem cells. But this method may not work because the cells may migrate out of the scalp too quickly. So AAPE is the best bet.

if you think it's a good strategy that more forum user send mails to the clinic, why not just create some fake mail addresses on your own from gmail, hotmail, gmx etc. so you could have a lot of different identity's and appear as many different persons? you could easily create 20, 50 or even 100 different accounts which could be used to email them. of course you should adapt the text every time a bit so that they have the feeling of talking to different persons.
you would then see if your strategy pans out. if not, at least it was worth a try and you can move on.

[hgs1989]

On the role of ADSC in hair regeneration, horsley of yale university made a study after the one which they discovered the thinning of fatty layer during catagen. http://horsley.yale.edu/sites/defaul...s/bsreview.pdf interestingly they further supported the importance of ADSC on hair. at many occasions they showed that mice lacking a proper fatty layer had had hair loss and early entry into catagen. the more intersting part is that " transplantation of adipocyte progenitor cells intradermally into the back skin of shaved mice at the extended 3–4 week telogen stage of the hair follicle cycle that occurs around 7 weeks of age resulted in adipocyte graft formation and corresponding precocious hair growth. Anagen was induced in these mice injected with the enriched adipocyte progenitor cells from WT or AZIP, but not with cells of the entire stromal vascular fraction. , supporting that the hair-inducing activity was specific
for immature adipocyte lineage cells."

so transplantation of adepocyte progenitor cells intradermally cause the hair to enter anagen phase prematurely. most clinics that are studying this are working with SVF. the papaer notes that only immature cells are needed. according to this: http://stemcellassays.com/2013/05/breaking-fat-svf/ only 2 - 10% of SVF is stem cells.
some of you brought the problem of migration adding this to the low concentration of the needed cells make it worse.
Dr.yates recently posted hair transplant results with an injection of SVF+prp after 7 months. either that the transplant was really really good or that SVF+prp played a big role in thickening the guy's hair. what do yo guys think?

some notes before replying to this:
1-I realize that yale study is on mice, but remember replicel, histogen, christiano, jahoda, lauster and everybody else worked with mice first.
2- my personal take on 1 is that what affects mice positively and causes great results will not be the full head of hair for us but it will affect us positively for sure. our hair is much more complicated than mice's fur.

another thing I wold like to point out is that some of you question the role of prp. I would rather question current practice. charging > 1000$ and market it as a one time a year treatment while it has some potential with several injections. I found this article that might support this http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032742/ same with SVF. the link I posted above notes that only a small fraction of SVF contains stem cells. so multiple injections would also be required.

[nameless]

On the role of ADSC in hair regeneration, horsley of yale university made a study after the one which they discovered the thinning of fatty layer during catagen. http://horsley.yale.edu/sites/defaul...s/bsreview.pdf interestingly they further supported the importance of ADSC on hair. at many occasions they showed that mice lacking a proper fatty layer had had hair loss and early entry into catagen. the more intersting part is that " transplantation of adipocyte progenitor cells intradermally into the back skin of shaved mice at the extended 3–4 week telogen stage of the hair follicle cycle that occurs around 7 weeks of age resulted in adipocyte graft formation and corresponding precocious hair growth. Anagen was induced in these mice injected with the enriched adipocyte progenitor cells from WT or AZIP, but not with cells of the entire stromal vascular fraction. , supporting that the hair-inducing activity was specific
for immature adipocyte lineage cells."

so transplantation of adepocyte progenitor cells intradermally cause the hair to enter anagen phase prematurely. most clinics that are studying this are working with SVF. the papaer notes that only immature cells are needed. according to this: http://stemcellassays.com/2013/05/breaking-fat-svf/ only 2 - 10% of SVF is stem cells.
some of you brought the problem of migration adding this to the low concentration of the needed cells make it worse.
Dr.yates recently posted hair transplant results with an injection of SVF+prp after 7 months. either that the transplant was really really good or that SVF+prp played a big role in thickening the guy's hair. what do yo guys think?

some notes before replying to this:
1-I realize that yale study is on mice, but remember replicel, histogen, christiano, jahoda, lauster and everybody else worked with mice first.
2- my personal take on 1 is that what affects mice positively and causes great results will not be the full head of hair for us but it will affect us positively for sure. our hair is much more complicated than mice's fur.

another thing I wold like to point out is that some of you question the role of prp. I would rather question current practice. charging > 1000$ and market it as a one time a year treatment while it has some potential with several injections. I found this article that might support this http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4032742/ same with SVF. the link I posted above notes that only a small fraction of SVF contains stem cells. so multiple injections would also be required.

That's interesting about PRP. I guess there is some small benefit to PRP but I really am not very interested in PRP as it stands. Maybe they should try even more than 3 injections and see if say 6 or 7 injections improve on the results even more than 3 injections do.

That aside, I still have concerns about injected ADSCs migrating out of the scalp after injection. Yhe world-class heart clinic working with ADSCs in The Caribbean indicated that studies show that essentially the ADSCs injected into hearts , and other tissue, migrated out of that tissue within 3 days. Some of the cells could still be found in the tissue at 5 weeks although it appears that the overwhelming majority of these cells migrate out of the tissue within 3 days. At the bottom of this email I will post the email from that heart clinic rep to me.

I agree with you about SVF. I think it's a waste of time. Not only is there very little ADSCs in the mix but also some of the other things in the mix could actually inhibit the ADSCs.

Based on all of the above I would say AAPE is our best bet for a near term solution. I do think that there's a chance that ADSCs might be effective but if the stay in the target area 3 days or less I think AAPE is the smarter choice between these 2. For one thing, the mere act of harvesting these cells, culturing them, and then re-implanting them is enough to throw them off-kilter for a short spell. The shock of hair transplants can affect the growth cycle of follicles so why not ADSCs? I can imagine that it could easily take 3 days or longer for the ADSCs to get re-acclimated after being harvested, cultured, and re-implanted back into the skin. I think that AAPE is the thinking man's selection over ADSCs since the cells migrate out of tissue rapidly.

Also, thanks for the added Yale study demonstrating that the right cells alone can prompt hair growth in mice. It's another piece of evidence proving that AAPE is what we should be focusing on.

[nameless]

hgs1989 here again is that email from the ADSC heart clinic talking about how the ADSCs essentially leave the target area within 3 days:

ADRCs as well as mesenchymal cells (MSCs) from bone marrow,when delivered in the heart as well as other tissues and organs have generally migrated out of the target tissue after about three days though some can be found up to about five weeks. It is important to note that the primary way these cells regenerate and repair is through cell-to-cell signaling (paracrine effect). The cells can and do turn into other cells. That is why they are called adult stem cells. The differentiate into adipose, bone, cartilage etc. The cells “know” what tissue is needed so in the case of the heart they differentiate into heart muscle cells (cardiomyocytes). It is also important to note that while ADRCs have been found to grow new cardiomyocytes they are not enough to build new muscle but they appear to assist in improving pumping function. The most important mechanism for cardiac and other wound repair found with ADRCs is the growth of new blood vessels (angiogenesis). These new bloods vessels support the main arteries and bring more blood and oxygen to the lungs so the person can breathe better per the PRECISE trial (see data on maximum volume of oxygen consumption –mVO2).
What makes ADRCs “better” is that they have been found to be more abundant with cells that are more directly capable of stimulating the growth of new blood vessels as well as cardiomyocytes and are more potent later in life that bone marrow cells.

[nameless]

hgs did you see this:

http://www.youtube.com/watch?v=vrz3Rp4JwDM

[nameless]

hgs1989 I don't think that this could possibly be what we're looking for because these growth factors and proteins have to stored and formulated in specific ways in order for them to be effective. Even Dr. Aaron Gardner said they're unstable so I don't see how they could be put in a bottle and just sit on a shelf till someone buys them but I thought you might want to see this:

http://ymsconsulting.org/aape_faq.html