Dr Nigam, my own experience

[Conpecia]

Haha how pissed are all these Replicel/Aderans/Histogen/Tsuji guys gonna be at Nigam??? But hey that's life, swim or drown. Nigam's stroking away...

[yan]

Haha how pissed are all these Replicel/Aderans/Histogen/Tsuji guys gonna be at Nigam??? But hey that's life, swim or drown. Nigam's stroking away...

Could someone please enlighten me, why you guys are all that euphoric? Nothing has been proofed so far!?

[hiilikeyourbeard]

Could someone please enlighten me, why you guys are all that euphoric? Nothing has been proofed so far!?

you realize that having forum members of the biggest hairloss site on the planet try this out is huge, right? he has to be completely confident in it's ability. cause if it doesn't work, we'll all know. that in itself is pretty exciting

[dipship31]

Could anyone give me a quick summary what's going on here? Went back a few pages in the thread and it looks like there is a ton of uptimism! Could anyone give me a quick paragraph overview of why??

[yeahyeahyeah]

Could anyone give me a quick summary what's going on here? Went back a few pages in the thread and it looks like there is a ton of uptimism! Could anyone give me a quick paragraph overview of why??

Long story short , a bunch of guys think nigam is legit

[Arashi]

Long story short , a bunch of guys think nigam is legit

Nah, long story short: Jahoda et al managed to biogenerate Human Hair follicles, using 3d spheres to grow DP cells, on human skin on SCID mouse( http://www.baldtruthtalk.com/showpos...&postcount=116 ). Now forum member Boldy, who's a biotech guy and Dr Nigams are going to try to repeat this, on Boldy himself, on another forum member and on Tom. If they can repeat what Jahoda did, generate hair follicles, then .... we'll you know what party time

[534623]

Went back a few pages in the thread and it looks like there is a ton of uptimism! Could anyone give me a quick paragraph overview of why??

Sorry, I don't know why either.

[One]

TYTR,
You can see the difference between the lateral slits or holes by traditional fue implantation at the recipient,
versus the needle based implantation of the follicle at the recipient mpb scalp,
when , i will use the same for bisected graft implantation by needle for BOLDY(the forum member) .

At present in hair transplant procedure,
recipient implantation of grafts is performed by using needle or surgical blades to create holes at the recipient,
The graft is than inserted/pushed against the outflowing blood,into the dermis with the help of forcep..

This process ,is not able to avoid microscarring,scabs,minimum distance required between the two grafts implanted at the recipient.

CHOIR or PEN implants are available..but still one has to push the grafts into the hole of the needle,more than two follicle grafts have issues with use of choir implanters..
.
When the surgeon makes a hole at the recipient and push the graft into the hole ,the outflow of blood,and the force used to push the grafts can lead to some damage to the grafts...apart from the obvious scabs and microscarring.

What i have developed now... is an intra dermal needle,with a slit to place the graft...and this needle is connected to the syringe...the syringe has a fine wire within ...when the needle is inserted into the scalp... the piston of the syringe is pushed..the fine wire within,enters the needle..and pushes the grafts into the skin..
I call it...SCARLESS NEEEDLE IMPLANTER
As you know when you take an intradermal injection...there is no mark,no microscarring,faster healing...and definitely high density,less damage to the grafts ,less damage to the blood vessels,less proping out of grafts...,no scabs,faster way to get rid of the redness,and hopefully better chance of graft survival after implantation..!

Wow Dr Nigam you invent a discovery per month! How come no other surgeon in 10 years of FUE one has ever thought of that? And yet it would be useful to them ...

Can you give us more details ...

I'm sorry but it seems too good to be true ...

[KO1]

Could someone please enlighten me, why you guys are all that euphoric? Nothing has been proofed so far!?

We have a knowledgeable forum member in Dr Nigam's lab, running experiments. Given that many on this forum have expressed doubts about Dr Nigam, having a respected member physically in his lab will make people much happier.

[hellouser]

Nah, long story short: Jahoda et al managed to biogenerate Human Hair follicles, using 3d spheres to grow DP cells, on human skin on SCID mouse( http://www.baldtruthtalk.com/showpos...&postcount=116 ). Now forum member Boldy, who's a biotech guy and Dr Nigams are going to try to repeat this, on Boldy himself, on another forum member and on Tom. If they can repeat what Jahoda did, generate hair follicles, then .... we'll you know what party time

I wish we knew the results immediately after, instead we're going to have wait at least a couple months before terminal hairs are visible

[Vox]

Nothing has been proofed so far!?

And nothing would be for many years to come, if we had not some knowledgeable members here going to Dr Nigam's clinic in order to test his claims on themselves. Seeing the outcome of the cutting-edge methods that are being tested, be it positive or negative, is huge by itself.

[caddarik79]

Hey guys,


Indeed I'm in mumbai currently in a hotel.

here is some experience.


My purpose if this visit, is to let dr nigams experiment with different DP culture protocols to get the max of cell therapy.

I have different protocol's , including 3d culture, to aggregate the cells prior injection.

Also we will be checking, and optimizing the current 2d culture, to expand the cells at higher rate. My goal is to achieve approximately 5 doubling per passage/ (7-10 days).


50% of the extracted cells will be cultured by his own protocol, the other half, by the new protocol, and we will see difference in growth very soon between the protocols under the inverted microscope to compare the results.

- at the end, the winning protocol will be the new standard, based on alp activity, lef1, and proliferation speed.

to ensure the cell trichoniciy. at the end, the expanded cells will be aggregated in to small spheroids in 3d culture, for the multiple 3d culture different methods will be tested. hanging drop is one of them.

- also some extra grafts where taken to do his normal Hm method(in a different culture flask), that contains multiple cells, from the bulge, ors, ds/dsc etc) all these cells are trichogenic.

this combination (nigams current HM (including epithelial cells) + improved expanding of the dp's in 2d and aggregating in 3d culture as last step, will be something that no one has tried before (aderans + replicel).

I have faith in this.


You guys all know that I'm sensitive, and can't tolerate drugs. Today we found out that my blood doesn't cloth at all. we will look tomorrow how to solve it, before we can do a Doubling for the hairline.

now this was the technical stuff, that I find interesting to talk about.


the flight, was very nice (by french air) everything went easy. there at mumbai, the pickup guy with DR. Nigams t shirt was standing with a big DR nigams picture board haha.. so it was not hard to miss that. he drove me from the airport to the hotel.

Next day (monday), piked up by the same (I will call it nigam's taxi), and brought to the clinic, to discuss some detials regarding dp culture and the protocols etc.

TOday we have done the above (extracting for hm and my dp protocol).


Im ultra curious to the cell growth of the different culture protocols, ..


I will probably do like 1500-2000 Doubling for the hairline, to breing it back to its original state, like what tom has. and my dp protocol + nigams Hm, is meant to give super high density.



the +-175 extracts (for the cell culture), nigams told me I don't want to leave the holes in the donor in this state,lets go for some regrowth there. lets use body hair' disassociated dermal papilla cells + tomorrow, some% of the Hm cells (he calls this activated stemcells I guess..).

The have fist used a numbing ointment, then ice, and then anesthesia, so I was not able to feel the extraction.

I have 2 goals, and I don't know at this point which I give the first place. Both are as important for me.

- Get my nw0 back at full density
- Working on something that will make Ht's/ and mechanical stuff History, since I believe that cell therapy is the cure and can be done efficient.


for the newbies that don't know what cell therapy is: http://www.hairlosshelp.com/forums/m...VIEWTMP=Linear


I will try to keep you guys updated with my experience if there is need for that.

WELL, BEST OF LUCK to you, I hope indeed that HT or other false solutions will be dismissed by one final cutting edge and definitive treatment.... it's been decades that balding people have been exploited by criminal (docs, labs) who only make money on desperation.

and 2013 or 2014 or even 2015 sounds realistic (but I will keep quiet) for it since I remember people hoping for the holy grail being ready in the very early 2000... I even remember forum members of 2002 being sure (about cloning, HM) it would be MAXIMUM 2010, the LAAAATEST... so 2014 or 2015 seems right.

[walrus]

Lets not get carried away until there is EVIDENCE that it actually works.

[clandestine]

edit: nvm

Boldy if you end up curing hair loss with Nigams, well, that'd be just swell.

[tom vercetti]

Boldy,

I ask my question to you this time: what do you think is the best way to inject the 3D DP cells into the scalps ? I mean, about the deap etc.. In order to not damage the bulge and to reach a large area of hair ?