Dr Nigam, my own experience

[Arashi]

Dr Nigams, on HS you said you're going to experiment with the 3d culture on those 2 forum members, right ? If I understand correctly, you're going to try to repeat Jahoda's experiment with SCID mice but this time on them ?

[TO YOUNG TO RETIRE]

thanks for the detailed answer Dr. but doesnt this increase the time of the procedure? and how close can you implant the grafts each other?Dr i think you are changing the transplant field by a lot

TYTR,
You can see the difference between the lateral slits or holes by traditional fue implantation at the recipient,
versus the needle based implantation of the follicle at the recipient mpb scalp,
when , i will use the same for bisected graft implantation by needle for BOLDY(the forum member) .

At present in hair transplant procedure,
recipient implantation of grafts is performed by using needle or surgical blades to create holes at the recipient,
The graft is than inserted/pushed against the outflowing blood,into the dermis with the help of forcep..

This process ,is not able to avoid microscarring,scabs,minimum distance required between the two grafts implanted at the recipient.

CHOIR or PEN implants are available..but still one has to push the grafts into the hole of the needle,more than two follicle grafts have issues with use of choir implanters..
.
When the surgeon makes a hole at the recipient and push the graft into the hole ,the outflow of blood,and the force used to push the grafts can lead to some damage to the grafts...apart from the obvious scabs and microscarring.

What i have developed now... is an intra dermal needle,with a slit to place the graft...and this needle is connected to the syringe...the syringe has a fine wire within ...when the needle is inserted into the scalp... the piston of the syringe is pushed..the fine wire within,enters the needle..and pushes the grafts into the skin..
I call it...SCARLESS NEEEDLE IMPLANTER
As you know when you take an intradermal injection...there is no mark,no microscarring,faster healing...and definitely high density,less damage to the grafts ,less damage to the blood vessels,less proping out of grafts...,no scabs,faster way to get rid of the redness,and hopefully better chance of graft survival after implantation..!

[tom vercetti]

I will also get my 3D DP cells injected by next week ! hope it will improve the density all over my scalp and get a Cristiano Ronaldoooooooo full density

[Boldy]

FTL,
The european doc would most likely, be coming on 2nd september.
Tom is coordinating the meet..

I have not heard back from dr cole /spencer..
you will have to ask them..regards further news...

Wesley belgium and boldy, the forum members are here with me at my clinic..
They will be getting hair doubling and HM.

Wesley and boldy will receive incision less recipient implantation with my new needle based implantation .

Hey guys,


Indeed I'm in mumbai currently in a hotel.

here is some experience.


My purpose if this visit, is to let dr nigams experiment with different DP culture protocols to get the max of cell therapy.

I have different protocol's , including 3d culture, to aggregate the cells prior injection.

Also we will be checking, and optimizing the current 2d culture, to expand the cells at higher rate. My goal is to achieve approximately 5 doubling per passage/ (7-10 days).


50% of the extracted cells will be cultured by his own protocol, the other half, by the new protocol, and we will see difference in growth very soon between the protocols under the inverted microscope to compare the results.

- at the end, the winning protocol will be the new standard, based on alp activity, lef1, and proliferation speed.

to ensure the cell trichoniciy. at the end, the expanded cells will be aggregated in to small spheroids in 3d culture, for the multiple 3d culture different methods will be tested. hanging drop is one of them.

- also some extra grafts where taken to do his normal Hm method(in a different culture flask), that contains multiple cells, from the bulge, ors, ds/dsc etc) all these cells are trichogenic.

this combination (nigams current HM (including epithelial cells) + improved expanding of the dp's in 2d and aggregating in 3d culture as last step, will be something that no one has tried before (aderans + replicel).

I have faith in this.


You guys all know that I'm sensitive, and can't tolerate drugs. Today we found out that my blood doesn't cloth at all. we will look tomorrow how to solve it, before we can do a Doubling for the hairline.

now this was the technical stuff, that I find interesting to talk about.


the flight, was very nice (by french air) everything went easy. there at mumbai, the pickup guy with DR. Nigams t shirt was standing with a big DR nigams picture board haha.. so it was not hard to miss that. he drove me from the airport to the hotel.

Next day (monday), piked up by the same (I will call it nigam's taxi), and brought to the clinic, to discuss some detials regarding dp culture and the protocols etc.

TOday we have done the above (extracting for hm and my dp protocol).


Im ultra curious to the cell growth of the different culture protocols, ..


I will probably do like 1500-2000 Doubling for the hairline, to breing it back to its original state, like what tom has. and my dp protocol + nigams Hm, is meant to give super high density.



the +-175 extracts (for the cell culture), nigams told me I don't want to leave the holes in the donor in this state,lets go for some regrowth there. lets use body hair' disassociated dermal papilla cells + tomorrow, some% of the Hm cells (he calls this activated stemcells I guess..).

The have fist used a numbing ointment, then ice, and then anesthesia, so I was not able to feel the extraction.

I have 2 goals, and I don't know at this point which I give the first place. Both are as important for me.

- Get my nw0 back at full density
- Working on something that will make Ht's/ and mechanical stuff History, since I believe that cell therapy is the cure and can be done efficient.


for the newbies that don't know what cell therapy is: http://www.hairlosshelp.com/forums/m...VIEWTMP=Linear


I will try to keep you guys updated with my experience if there is need for that.

[KO1]

Boldy, screw IT, you need to do a PhD in this. Go ask Jahoda if he wants to be your Advisor.

Best of luck, this is awesome.

[tom vercetti]

Hi boldy, so I will meet you in person by next week ! Im coming by 25th. Are you confident for my injection ?

I also trust a lot in this protocol

[TO YOUNG TO RETIRE]

lets all wish the best to boldy.. i hope it works out.

suddently we can see the light

[hellouser]

Boldy is gonna be be f.vcking crazy amounts of p.ussy

[Boldy]

Hi boldy, so I will meet you in person by next week ! Im coming by 25th. Are you confident for my injection ?

I also trust a lot in this protocol

Nice ! I'm confident that your cells will be aggregated in 3d. Yes(regarding all the papers I have read for human dp's). It should restore the trichoncity, and alp + lef1.

However, It will become more interesting soon, once we see the results of the new protocols.. less expanding time, more cells, and I assume even no loss of the lef1 and alp..., in 2d culture, which means the cells can be even trichonic in 2d as well..

I will try to enjoy mumbai also and maybe other cities, so its not just hairloss ...

btw, the women here, are pretty hot....

edit, I will try to make as natural as possible posts, which means I will post evry single negative Point I notice, so that other users if they ever plan to visit, know what to expect.

so far, I have to say, the culture here is different, the traffic here is allot different then europe... its a very busy city.. and the streets are not as clean as in europe!, However, I have seen them cleaning like 4-5 times today. there is some cleaning lady in the clinic, that cleans the whole day. air conditioning is installed in evry room in the hotel and the clinic.

[tom vercetti]

Nice ! I'm confident that your cells will be aggregated in 3d. Yes(regarding all the papers I have read). It should restore the trichoncity, and alp + lef1.

However, It will become more interesting soon, once we see the results of the new protocols.. less expanding time, more cells, and I assume even no loss of the lef1 and alp..., in 2d culture, which means the cells can be even trichonic in 2d as well..

I will try to enjoy mumbai also and maybe other cities, so its not just hairloss ...

Wow very nice ! thanks for the help you give to dr nigam and indirectly to me

[Arashi]

Boldy, just wanted to congratulate you with everything you're doing !! It's going to be extremely interesting to follow your findings, and that's even an understatement All the best !!

[Arashi]

Also Boldy, what do you expect ? I mean, if everything works out and you and dr Nigams can expand cells in 3d and elicit HF neogenesis (much like Jahoda did on human skin on SCID mice), then you and Dr Nigams have basically solved the hairloss puzzle and we can all get to NW0 ? Or am I missing something ?

[Boldy]

Also Boldy, what do you expect ? I mean, if everything works out and you and dr Nigams can expand cells in 3d and elicit HF neogenesis (much like Jahoda did on human skin on SCID mice), then you and Dr Nigams have basically solved the hairloss puzzle and we can all get to NW0 ? Or am I missing something ?

hmm, In theory it is, however this is the right time, to see what can be archived in real life, (I have waited long time for this).

To be honest, I know the cells will be trichonic, in 3d and even 2d.. the combo with what I have described in my earlier post that has not done yet, is also very encouraging..

Somehow I feel its my responsibility is to make the best and fine tune the culture methods like explained in my earlier post in page 85, and real life data should tell us, how much repeated treatments, should be needed for good density.


I believe in everything is possible.. , the question remains: all this energy and time put in this project enough to solve or cure our hairloss?

Lets go and find out.

[Arashi]

hmm, In theory it is, however this is the right time, to see what can be archived in real life, (I have waited long time for this).

To be honest, I know the cells will be trichonic, in 3d and even 2d.. the combo with what I have described in my earlier post that has not done yet, is also very encouraging..

Somehow I feel its my responsibility is to make the best and fine tune the culture methods like explained in my earlier post in page 85, and real life data should tell us, how much repeated treatments, should be needed for good density.


I believe in everything is possible.. , the question remains: all this energy and time put in this project enough to solve or cure our hairloss?

Lets go and find out.

Big props to you and Dr Nigams ! We're all praying here for you guys

[LMS]

hmm, In theory it is, however this is the right time, to see what can be archived in real life, (I have waited long time for this).

To be honest, I know the cells will be trichonic, in 3d and even 2d.. the combo with what I have described in my earlier post that has not done yet, is also very encouraging..

Somehow I feel its my responsibility is to make the best and fine tune the culture methods like explained in my earlier post in page 85, and real life data should tell us, how much repeated treatments, should be needed for good density.


I believe in everything is possible.. , the question remains: all this energy and time put in this project enough to solve or cure our hairloss?

Lets go and find out.

You along with Tom are pioneers. You got balls.