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you're a legend my friend. you are going to a place no man has gone before. if all this will work out great for you, ga duimen als een malle, then this will be epic beyond believe. good luck!Hey guys,
Indeed I'm in mumbai currently in a hotel.
here is some experience.
My purpose if this visit, is to let dr nigams experiment with different DP culture protocols to get the max of cell therapy.
I have different protocol's , including 3d culture, to aggregate the cells prior injection.
Also we will be checking, and optimizing the current 2d culture, to expand the cells at higher rate. My goal is to achieve approximately 5 doubling per passage/ (7-10 days).
50% of the extracted cells will be cultured by his own protocol, the other half, by the new protocol, and we will see difference in growth very soon between the protocols under the inverted microscope to compare the results.
- at the end, the winning protocol will be the new standard, based on alp activity, lef1, and proliferation speed.
to ensure the cell trichoniciy. at the end, the expanded cells will be aggregated in to small spheroids in 3d culture, for the multiple 3d culture different methods will be tested. hanging drop is one of them.
- also some extra grafts where taken to do his normal Hm method(in a different culture flask), that contains multiple cells, from the bulge, ors, ds/dsc etc) all these cells are trichogenic.
this combination (nigams current HM (including epithelial cells) + improved expanding of the dp's in 2d and aggregating in 3d culture as last step, will be something that no one has tried before (aderans + replicel).
I have faith in this.
You guys all know that I'm sensitive, and can't tolerate drugs. Today we found out that my blood doesn't cloth at all. we will look tomorrow how to solve it, before we can do a Doubling for the hairline.
now this was the technical stuff, that I find interesting to talk about.
the flight, was very nice (by french air) everything went easy. there at mumbai, the pickup guy with DR. Nigams t shirt was standing with a big DR nigams picture board haha.. so it was not hard to miss that. he drove me from the airport to the hotel.
Next day (monday), piked up by the same (I will call it nigam's taxi), and brought to the clinic, to discuss some detials regarding dp culture and the protocols etc.
TOday we have done the above (extracting for hm and my dp protocol).
Im ultra curious to the cell growth of the different culture protocols,
..
I will probably do like 1500-2000 Doubling for the hairline, to breing it back to its original state, like what tom has. and my dp protocol + nigams Hm, is meant to give super high density.
the +-175 extracts (for the cell culture), nigams told me I don't want to leave the holes in the donor in this state,lets go for some regrowth there. lets use body hair' disassociated dermal papilla cells + tomorrow, some% of the Hm cells (he calls this activated stemcells I guess..).
The have fist used a numbing ointment, then ice, and then anesthesia, so I was not able to feel the extraction.
I have 2 goals, and I don't know at this point which I give the first place. Both are as important for me.
- Get my nw0 back at full density
- Working on something that will make Ht's/ and mechanical stuff History, since I believe that cell therapy is the cure and can be done efficient.
for the newbies that don't know what cell therapy is: http://www.hairlosshelp.com/forums/m...VIEWTMP=Linear
I will try to keep you guys updated with my experience if there is need for that.Comment
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Boldy good luck man ...
thanks for doing this for our community! I hope that your case will open a new page in the history of hair loss.Comment
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Wow, if this all works out as good as it sounds in theory, then we might see real cure for baldness (or at least a very effective treatment) very soon! This is just crazy! Boldy, Tom Vercetti, Wesley, Dr. Nigam, good luck! I sooo hope this is going to work!
Exciting times ahead of us
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THIS IS ****ING AWESOME!! HELL YES TO BOLDY/TOM/DR. NIGAM!!! We are on the razor's edge my friends!!!Comment
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All we need is to see proper well documented results on Boldy, Tom Vercetti and Wesley. We don't need no Dr. Cole.
Besides since Dr. Cole is HT surgeon, his eventual negative opinion might be false, because if Nigam is legit, it would be a HUGE problem for his business (and also business of all HT docs in the world).Comment
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please work please work please work please workComment
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No it won't be. You underestimate the predictability of human stupidity. Even if there were a flat out complete cure reversing NW7 to NW0, there will still be enough ignorant people to come streaming into Dr. Cole's clinic ready to whip out heaps of cash for stone age treatments. Case in point? LeBron James. His hairline is fading and you can clearly see a strip scar on the back of his head where there never was one. He could have opted for scarless FUE. He could have opted for Dr. Gho. Even with all his millions of dollars, this dude goes for the worst option on the market.
Cole isn't going to lose business overnight.
But, if he has any plans on being relevent for a while, he damn well better take what Dr. Nigams is doing and use it to his advantage... otherwise, he's going to see his business slowly fold. So, either shape up, or ship out.
I still can't believe finasteride is being sold as a treatment... 2013 and we're still waiting for a fvcking cure and we're only now talking about the possibility of a cure and the credibility of Dr. Nigams. Just PATHETIC.Comment
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Damn! Now that is some cutting edge / future technology gentlemen!
So would you get just one follicular unit from each spheroid ball of cells?
Good Luck!Comment
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i don't think anyone really knows yet. Tsuji Labs (Team Tokyo) produced 124 hair shafts from 28 hair germs which was implanted within 1cm2 in their mouse model. that's over 4 hairs for every hair germ but they did not mention how many follicular units.
perhaps when the latest Higgins/chistiano/jahoda paper is released of 3d culturing we might know a little moreComment
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