Dr Nigam, my own experience

[TO YOUNG TO RETIRE]

lets all wish the best to boldy.. i hope it works out.

suddently we can see the light

[tom vercetti]

Hi boldy, so I will meet you in person by next week ! Im coming by 25th. Are you confident for my injection ?

I also trust a lot in this protocol

[KO1]

Boldy, screw IT, you need to do a PhD in this. Go ask Jahoda if he wants to be your Advisor.

Best of luck, this is awesome.

[Boldy]

FTL,
The european doc would most likely, be coming on 2nd september.
Tom is coordinating the meet..

I have not heard back from dr cole /spencer..
you will have to ask them..regards further news...

Wesley belgium and boldy, the forum members are here with me at my clinic..
They will be getting hair doubling and HM.

Wesley and boldy will receive incision less recipient implantation with my new needle based implantation .

Hey guys,


Indeed I'm in mumbai currently in a hotel.

here is some experience.


My purpose if this visit, is to let dr nigams experiment with different DP culture protocols to get the max of cell therapy.

I have different protocol's , including 3d culture, to aggregate the cells prior injection.

Also we will be checking, and optimizing the current 2d culture, to expand the cells at higher rate. My goal is to achieve approximately 5 doubling per passage/ (7-10 days).


50% of the extracted cells will be cultured by his own protocol, the other half, by the new protocol, and we will see difference in growth very soon between the protocols under the inverted microscope to compare the results.

- at the end, the winning protocol will be the new standard, based on alp activity, lef1, and proliferation speed.

to ensure the cell trichoniciy. at the end, the expanded cells will be aggregated in to small spheroids in 3d culture, for the multiple 3d culture different methods will be tested. hanging drop is one of them.

- also some extra grafts where taken to do his normal Hm method(in a different culture flask), that contains multiple cells, from the bulge, ors, ds/dsc etc) all these cells are trichogenic.

this combination (nigams current HM (including epithelial cells) + improved expanding of the dp's in 2d and aggregating in 3d culture as last step, will be something that no one has tried before (aderans + replicel).

I have faith in this.


You guys all know that I'm sensitive, and can't tolerate drugs. Today we found out that my blood doesn't cloth at all. we will look tomorrow how to solve it, before we can do a Doubling for the hairline.

now this was the technical stuff, that I find interesting to talk about.


the flight, was very nice (by french air) everything went easy. there at mumbai, the pickup guy with DR. Nigams t shirt was standing with a big DR nigams picture board haha.. so it was not hard to miss that. he drove me from the airport to the hotel.

Next day (monday), piked up by the same (I will call it nigam's taxi), and brought to the clinic, to discuss some detials regarding dp culture and the protocols etc.

TOday we have done the above (extracting for hm and my dp protocol).


Im ultra curious to the cell growth of the different culture protocols, ..


I will probably do like 1500-2000 Doubling for the hairline, to breing it back to its original state, like what tom has. and my dp protocol + nigams Hm, is meant to give super high density.



the +-175 extracts (for the cell culture), nigams told me I don't want to leave the holes in the donor in this state,lets go for some regrowth there. lets use body hair' disassociated dermal papilla cells + tomorrow, some% of the Hm cells (he calls this activated stemcells I guess..).

The have fist used a numbing ointment, then ice, and then anesthesia, so I was not able to feel the extraction.

I have 2 goals, and I don't know at this point which I give the first place. Both are as important for me.

- Get my nw0 back at full density
- Working on something that will make Ht's/ and mechanical stuff History, since I believe that cell therapy is the cure and can be done efficient.


for the newbies that don't know what cell therapy is: http://www.hairlosshelp.com/forums/m...VIEWTMP=Linear


I will try to keep you guys updated with my experience if there is need for that.

[tom vercetti]

I will also get my 3D DP cells injected by next week ! hope it will improve the density all over my scalp and get a Cristiano Ronaldoooooooo full density