50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

[didi]

Yes, I have seen the pic on HS,

You sayiing 80% regrowth,
we need to know what was extracted(type of grafts) and compare it to what is growing back(do 3 hair grafts grow as 3 hair grafts)

also do you know where you planted those grafts in recipient?

its good to see you are about to crack Ghos technique,


can you upload the pictures on TBT forum please, HS is pain to navigate

[drnigams]

Didi,I have mailed before (large ) pics of donor to NSN including the before pics of birth mark.
Not just 2 follicle grafts but he may get extra regeneration both at donor and recipient because we injected dp cells,dp,activated stemcells,growth factors and ecm.
BTW it is not 80% ,one graft is not in the view in the photo,90% regeneration is what i am expecting around birth mark and am i waiting for the last extraction and would like to watch it some more time.
Some times due to human error technician may end up extracting the whole graft.You can see the healing which is clean and very quick.
Theoretically donor regeneration has to be 100%,practically due to human error it may range between 90 to 95% donor regeneration.
More imp is to watch the recipient regeneration,because it contains the bisected graft without the dermal papilla and blood supply.

You sayiing 80% regrowth,
we need to know what was extracted(type of grafts) and compare it to what is growing back(do 3 hair grafts grow as 3 hair grafts)

also do you know where you planted those grafts in recipient?

its good to see you are about to crack Ghos technique,


can you upload the pictures on TBT forum please, HS is pain to navigate[/QUOTE]

[didi]

Im impressed with regrowth in donor area(around bmark),
wow..90% regrowth ,it beats hst thats for sure...

I can see power shift...from Holland to India

[UK Boy]

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

[UK Boy]

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

Also why did you not consider DP culturing years ago when Aderans and Intercytex first mentioned it?

Will you also try culturing Dermal Sheath Cup Cells like Replicel as well or do yoy not think thst those cells will work? Are you allowed to/able to culture DSC cells?

Sorry if you have previously mentioned all this elsewhere. The cell growth side of your work is of more interest to me than all the hair transplant stuff.

[drnigams]

UKBoy,
Aderans are into autologous stemcell solution injections 6weeks apart.
We already have similar solution with similar results like aderans as on today.But these results need to be improved.
Aderans i think but not sure have recently also started dp culturing,i am not sure is the dp culture part of their clinical trial or not.Some one can find out and help me with that.
According to me the number one guy is the german Dr.Gerd than comes aderans,histogen,replica etc. who has already created hair germ in the lab and published that paper.
Since he is not a surgeon neither they are into clinical trials at present hence the effort has not resulted into commercial availability of hair germ implant.May be most probably i will take their work foraard into clinical trials or if hopefully get a sanction from indian regulators to allow me to use hair germ as part of medical procedure rather than as adrug ,in that case in 2014 we should be able to offer to the patients from our clinic in BOMBAY,India.
Another Scientist i am impressed with and will meet him this year is Takashi Tsuji, a regenerative medicine specialist of Tokyo university of science who created 95% growth on mice after injecting human hair stemcells.
Ofcourse the brilliant UK researcher Jahoda ,whom i am meeting in may2013, he injected his dp cells on his wife's arm and got 2 growing follicles.
Regarding regulations if you want to launch any stemcell product for mass use in the market...the full process of clinical trials has to be completed but if you are offering in a limited way under monitoring of doctors from ones clinic or hospital than in certain counties it is given permission as a medical procedure rather than classified as a drug.
We have the clearance from Independent Institutional committee for stemcell research and therapy with minimum manipulation as medical procedure from our specific clinic at mumbai and also clearance from Independent ethics Committee.
We have also register ourself with the regulatory authorities and informed the details of our process ,
we also need to summit all the records of the patients with followup to the regulatory authorities.
All our materials should be sourced from the FDA registered manufacturers or distributors including imported products .Which we comply with the bills and invoices.
Dr Gerd will help us as a consultant shortly and will also visit the mumbai lab when we finalize our contract next month.


With the help

Dr. Nigam, sorry to bother you again but I just wanted clarification.

When you talk about doing DP culturing is that the same as what Aderans do? I know you have different regulations hence why you can do this but I to ask how you think you can make this work when Aderans have spent years and years and millions of dollars and still only have a 60% response rate with only 40% getting any sort of cosmetically viable regrowth. What do you plan to do to make it work better?

In terms of going hair germs I wish you the best of luck, it would be excellent if you have success with this and could get the hair germs to sprout in vivo. Will Dr. Gerd be working in your lab in India full time or just consulting from Germany? If you have success what will be the next step? The treatment will be available in India but surely if will be wanted worldwide.

Thanks for your answers Dr. Nigam.

[UK Boy]

UKBoy,
Aderans are into autologous stemcell solution injections 6weeks apart.
We already have similar solution with similar results like aderans as on today.But these results need to be improved.
Aderans i think but not sure have recently also started dp culturing,i am not sure is the dp culture part of their clinical trial or not.Some one can find out and help me with that.
According to me the number one guy is the german Dr.Gerd than comes aderans,histogen,replica etc. who has already created hair germ in the lab and published that paper.
Since he is not a surgeon neither they are into clinical trials at present hence the effort has not resulted into commercial availability of hair germ implant.May be most probably i will take their work foraard into clinical trials or if hopefully get a sanction from indian regulators to allow me to use hair germ as part of medical procedure rather than as adrug ,in that case in 2014 we should be able to offer to the patients from our clinic in BOMBAY,India.
Another Scientist i am impressed with and will meet him this year is Takashi Tsuji, a regenerative medicine specialist of Tokyo university of science who created 95% growth on mice after injecting human hair stemcells.
Ofcourse the brilliant UK researcher Jahoda ,whom i am meeting in may2013, he injected his dp cells on his wife's arm and got 2 growing follicles.
Regarding regulations if you want to launch any stemcell product for mass use in the market...the full process of clinical trials has to be completed but if you are offering in a limited way under monitoring of doctors from ones clinic or hospital than in certain counties it is given permission as a medical procedure rather than classified as a drug.
We have the clearance from Independent Institutional committee for stemcell research and therapy with minimum manipulation as medical procedure from our specific clinic at mumbai and also clearance from Independent ethics Committee.
We have also register ourself with the regulatory authorities and informed the details of our process ,
we also need to summit all the records of the patients with followup to the regulatory authorities.
All our materials should be sourced from the FDA registered manufacturers or distributors including imported products .Which we comply with the bills and invoices.
Dr Gerd will help us as a consultant shortly and will also visit the mumbai lab when we finalize our contract next month.


With the help

All sounds very interesting and exciting Dr. Nigam. If your work with Dr. Gerd is successful it will be a huge leap forward and bring to hair loss sufferers a treatment that was not expected for another 10 years. You seem to good to be true Dr. Nigam and that worries me si I won't get my hopes up too much but I think that what you preach is very true - we will beat hair loss by working TOGETHER. By getting in touch with the other doctors who are workung on this problem and trying to work with them you are taking the industry forward hugely. It's ridiculous that it's taken someone so long to do this.

[Boldy]

All sounds very interesting and exciting Dr. Nigam. If your work with Dr. Gerd is successful it will be a huge leap forward and bring to hair loss sufferers a treatment that was not expected for another 10 years. You seem to good to be true Dr. Nigam and that worries me si I won't get my hopes up too much but I think that what you preach is very true - we will beat hair loss by working TOGETHER. By getting in touch with the other doctors who are workung on this problem and trying to work with them you are taking the industry forward hugely. It's ridiculous that it's taken someone so long to do this.

Good to see someone has the balls to step forward from the already well paid ht solutions, and trying something new that is the cure. (if its in real world as nice as mice/ paper)



@ nigams, arent the germcells created by 3d culture by putting dp and epithelial cells together? this procedure should allow new hair where its never excited before (from nw0 to nv negative for example) I remember a study where they combined epthelial cells + dp cells to create hair on a mice ear.
where DP cells alone couldn't do it since there where no KC cells.

Important to always do PCR screening to check for mutations, although they were not found in some studies even after 90 passages, its still a must.

regarding the procedure you are doing nigams, there is nothing special about it, its just basic cell culture/ biology if you ask me, so I wonder why the **** we had to wait all these years, while this procedure is known for at least 15 years.....

it makes me thinking that drug companies are behind this slow process....

If you bring this forward, even the haters on this forum will love you,

I'm sure

[drnigams]

Boldy,
Your knowledge of the subject fascinates me.
Can you mail me the links regarding 3d culturing studies for hair germ creation.I had discussed 3d culture with Dr Gerd.
We do culture Keratinocyte stemcells present in the bulge of follicle.
Can you elaborate more on the KC cell requirement for hair germ formation.
Regarding PCR screening ,yes we do process the samples in thermocycler and watch cells in GELDOC for any mutation.
You are right any biotec will understand regarding HM cell culturing is nothing big,and the real challenge is ahead to create potent dp culture,dp implant and hair germ invitro creation and regrowth of the same on human scalp.

Good to see someone has the balls to step forward from the already well paid ht solutions, and trying something new that is the cure. (if its in real world as nice as mice/ paper)



@ nigams, arent the germcells created by 3d culture by putting dp and epithelial cells together? this procedure should allow new hair where its never excited before (from nw0 to nv negative for example) I remember a study where they combined epthelial cells + dp cells to create hair on a mice ear.
where DP cells alone couldn't do it since there where no KC cells.
Regarding PCR screening ,yes we do process the samples in thermocycler and watch GELDOC for any mutation.
You are right any biotec will understand regarding HM cell culturing is nothing big,and the real challenge is ahead to create potent dp culture,dp implant and hair germ invitro creation and regrowth of the same on human scalp.


Important to always do PCR screening to check for mutations, although they were not found in some studies even after 90 passages, its still a must.

regarding the procedure you are doing nigams, there is nothing special about it, its just basic cell culture/ biology if you ask me, so I wonder why the **** we had to wait all these years, while this procedure is known for at least 15 years.....

it makes me thinking that drug companies are behind this slow process....

If you bring this forward, even the haters on this forum will love you,

I'm sure

[534623]

Ofcourse the brilliant UK researcher Jahoda ,whom i am meeting in may2013, he injected his dp cells on his wife's arm and got 2 growing follicles.

Dr. Nigam,
your knowledge of the subject - doesn't fascinate me.

[drnigams]

I am hungry for more facts ,
Ironman go ahead give more facts about jahoda's work,dermal papilla cells/potent culture and it's potential to create follicle,you seem to think that only cd34+ alone can create new follicles.
Talk about solutions ,let's help each other and work together.I know you know all and you won't need anybody's help..
By the way i will ask jahoda himself when i meet him in first week of MAY2013 and learn more about about his experience of effects and potential of dp cells/culture and doubling.
Ironman share your wisdom and help the industry to find MPB cure.
What do you suggest should the researchers focus on to create hair germ or in any other way create new follicles.You know doublings are interim procedures till we reach at successful invivo or invitro HM.

Dr. Nigam,
your knowledge of the subject - doesn't fascinate me.

[didi]

Whats the deal with dr Jahoda?
How come he hasnt progressed much since his discoveries

[UK_]

Iron_Man, why are you having such a tough time accepting your defeat?

Take it like a man dude, move on!

Dr Nigam is a hair loss surgeon working with some of the best names in the industry, and you're.... some loon on a hair loss forum?

[drnigams]

Didi,
Researchers like Jahoda ,Gerd,Takashi and others are lab researchers and they it leave for applied researchers like us,aderans to work on it clinically and offer to the the patients or sell their research to companies like aderans to monetize it.Applied researchers are key to integrate various different aspects of research for a new invention which should also be commercially viable.
Didi, basically the work of HT will today or tomorrow shift from HT doctors to Biotechs.
Because of this reason not lot of HT docs are interested to research on stemcells and even if they want unfortunately they are not trained in the field of biotechnology and they will need help of biotechs and will also need a lab.
Remember we SURGEONS LOVE SURGERY, without surgical process most of us feel incomplete, with few exceptions.
On the other hand biotechs can't inject anything on apatient as they are not licensed doctors and they will need help of HT docs for procedure and clinical follow up unless they are capable enough financially to risk in expensive clinical trials.
That's why even at the age of43 ,i enrolled for masters in biotech and finished my masters last year which definitely helps me
integrate the experience and knowledge as an HT doctor with the tissue engineering expertise , and ofcourse having a euippedFDA certified lab and clinic with HT patients together, does help .
I am just taking forward my thoughts and the research of great scientists forward by studying their published studies,interacting with them and applying some commonsense.

Lot of these researchers are not entrepreneurs but are employees and have no direct benefit commercially,many do research publish and sell their research and they may or may not have know how, as how to monetize their research and fund it further.
Unlike aderans who while developing on stem cell HM have already acquired bosley HT clinics ,they can use those clinics for trials and feeback and will have access to ready patients in 90 clinics across AMERICA when their HM product/process hits the market, thus they can survive with long term research with monetizing process in place unlike other talented scientists but without commercial vision.

Whats the deal with dr Jahoda?
How come he hasnt progressed much since his discoveries

[didi]

dr nigam
thats where enteprenuers like yourself come in to take care of applied science..
You could employ those scientists to work for you company, they dont have to physicaly relocate...you need some real brains..fast

I wonder why dr gho didnt make much progress in the past 8 years, he came up with HST which in reality is a form of FUE but nothing since then..he calls its stem cell transplantation but you could call FUE stemcell transplant...
im sure he tried to deliver true HM but didnt work in practice and now his focus is HSI..still not HM..just another flavour of HST

I lost hope in Aderans, i still remembr bout 6 years ago they said on their website to deliver HM before the end of decade(2010)...now it is 2013 ans still nothing..
i think you have better chances than aderans,
5 years from now is the best case scenario..in meantime we need cheap scarless doubling procedure