Dr. Nigam, Third Party Verification of Donor Doubling

[NeedHairASAP]

Two options to verify:

Question to the DR.

1. You said that, at a recent researcher event, one or two papers were presented that back donor doubling. Can you post these papers? Can you post about the researchers?

2. If not, can we (the forum and other crowdfunders) pay for a third party to do a 50-100 graft test?


question 2, part B:

If we could have a third-party ready to go tomorrow (hypothetically), who knew exactly how to perform the procedure to your current standard, HOW SOON CAN WE CONSIDER YOUR CLAIMS VERIFIED?

in my mind, I would think we could verify and count regrowth in both the donor and recipient within 3 weeks.


Question to the forum:

Why are we going to wait 2 years for him to turn a nw7 to an nw1? What does that prove? We could have a third-party do a 50-graft test and be done with this who thing. I can't imagine it would cost more than $35,000 usd-- which Arashi may cover almost half of-- and the rest which could maybe be crowdfunded.

[drnigams]

1. As requested kindly find below two of the few articles of study done independently..which confirms the possibility of donor doubling.
Add my hair stemcells and dp cells with or without growth factors for improved donor doubling'

New development in unlimited hair follicle transplant
C-M Lin1, K Huang2 and Y Li3 1Shantou University Medical College, Shantou, China; 2Second Affiliated Hospital, Shantou University Medical College, Shantou, China and 3First Affiliated Hospital, Shantou University Medical College, Shantou, China
Hair transplantation has had an important role in the treatment of hair loss. However, hair transplantation in fact represents hair redistribution. Thus, traditional hair transplantation is greatly restricted by insufficient transplants. Some studies have demonstrated that HF (hair follicle) upper fragments can regrow HFs. Despite the low success rate, hair graft number can be increased by hair follicle dissection. Here, we established a method to increase the regrowth rate of “single HF upper fragments.” HF upper fragments were obtained from the vibrissa of SpragueDawley rats at the level of hair bulb. Segments were transplanted underneath the skin of nude mice (6 segments per mouse, 1.5-cm distance between segments). Then culture medium of vibrissa HF was injected into the transplanted sites every 2 days, and grafts were excised at days 3, 7, and 9 and weeks 2 and 4. The control group received DMEM+10% fetal calf serum instead of HF culture medium. In all, 91.7% of the fragments (66/72) were able to regrow into intact HF from 7 days to 4 weeks. Only 2/38 of control mice fragments showed intact HF regrowth after 4 weeks of transplantation. We traced the expression of Wnt5a during the regrowth of HF: at 3 days after transplantation, the experimental group showed Wnt5a expression at the newly formed connective sheets. At 9 days, Wnt5a was expressed strongly at the typical newly formed DPs. We also designed a novel HF transplantation device that includes scissors at the bottom to dissect the HF bulb when obtaining HF from the donor. The remaining HF bulbs were able to regrow intact HFs in the donor sites. As well, the upper site of HFs can be used as hair graft transplants by the method above. Thus, our HF fragment transplantation method is a new development in unlimited hair follicle transplant.

This is what jahoida said in his presentation..after i spoke to him at edinburgh about the use of tissue engineering to double hair...as of now..tillpure HM based neofollicle creation becomes reality with consistence in the future.

Jahoida in his presentation at ishrs,snfransisco....
Other potential
developments
• The capacity of human follicle dermal cells to
induce follicles raises genuine possibilities for
creating tissue engineered skin grafts, and skin
equivalents that have the potential to develop hair
follicles and other skin appendages.
• The basic regenerative properties of the upper
follicle means that this phenomenon could be
combined with intact papilla follicle induction to at
least double follicle numbers.
Thanks and
Acknowledgments
DURHAM
Amanda Reynolds
Gavin Richardson
NEW YORK USA
Angela Christiano
Claire Higgins
DUNDEE
Roy


One more article apart from the two other independent studies on donor doubling posted few minutes back.

Involvement of the immune response in regeneration of experimentally amputated whisker follicles in vivo and in a novel culture model of regeneration using newborn follicles
S-W Li1,2, C Higgins3, K Sorensen2, AM Christiano3 and CA Jahoda2 1College of Life Science, Tarim University, Alar, China; 2School of Biological and Biomedical Sciences, Durham University, Durham, UK and 3Department of Dermatology, Columbia University, New York, USA
Follicles have a unique capacity to regenerate a functional end bulb after experimental amputation. Previous work suggests that regeneration entails an initial phase resembling skin wound healing, followed by specific remodeling of the bulb involving epithelialmesenchymal interactions. A key feature of the phenomenon is that the mesenchymal (dermal) components of the follicle (the dermal papilla and dermal sheath) reconstitute without scarring (fibrosis). Macrophages, as well as TGFβ family members, have been strongly linked with fibrosis; therefore one hypothesis is that lower follicle regeneration involves a reduced or modified immune response. This study aimed to investigate this question and to develop a novel culture model of follicle regeneration. For in vivo studies mouse and rat whisker follicles were experimentally amputated and follicles recovered between 24 hours and 3 weeks postoperatively. For in vitro work, follicles were isolated from newborn mice, bases amputated, and the follicles cultured on filters for up to 4 days. All specimens were cryofrozen or wax processed for immunohistochemistry/immunofluorescence. Early changes to the epithelium mirrored that of skin wound healing with migration of the follicle epithelium to fill the inner follicle silo, upregulation of cytokeratin15 near the follicle base, and strong expression of fibronectin. The in vitro model was able to undergo this first phase of regeneration. Labeling of in vivo specimens with a CD68 antibody (rat) and CD11b, F4/80 antibodies (mouse) identified highest concentrations of macrophages around the site of amputation at 48 hours, these cells being lost by 7 days. Interestingly, macrophages were also detected in the culture model. TGFβ1 was strongly expressed in follicle dermis, although, interestingly, not at the immediate site of dermal papilla regeneration. We suggest that lack of scarring in regenerated follicles cannot be attributed to lack of an immune response, although subtleties involving macrophage subpopulations are currently being investigated.

2.50/100 Grafts patch test of the three donor doubling anytime.
Anyway, i will be starting mr joshi's nw7 conversion to nw2 from 7th,8th and 9th november,as his cells will be ready by than.

crowd funding would be good idea, once i get jahoda, gerd, one member of tsuji,christiano,rajesh of cotsarelis group,stenn or washniek together..and use my lab in india with favourable regulations..for further research work to move close to cure with time lines...
I have already confirmed gerds participation,jahoda as agreed in principal,rajesh has also agreed in principal...i will talk to others in coming months.

we need to crowdfund to pay for a 50 graft test done by a third party service.

We could easily verify that things look promising at the 1 month mark-- and be sure of things at the 8-12 month marks....

waiting years for nigam to convert nw7 to nw2 is inefficient, and really not all the conclusive.

Nigam stated that papers were shown at the last research conference that confirm the principles behind his doubling procedure--- even this would be more conclusive in my eyes than the stupid 2 year long nw7-nw2 charade-- is it proof? yes. Is it efficient and timely? no.

Two options to verify:

Question to the DR.

1. You said that, at a recent researcher event, one or two papers were presented that back donor doubling. Can you post these papers? Can you post about the researchers?

2. If not, can we (the forum and other crowdfunders) pay for a third party to do a 50-100 graft test?


question 2, part B:

If we could have a third-party ready to go tomorrow (hypothetically), who knew exactly how to perform the procedure to your current standard, HOW SOON CAN WE CONSIDER YOUR CLAIMS VERIFIED?

in my mind, I would think we could verify and count regrowth in both the donor and recipient within 3 weeks.


Question to the forum:

Why are we going to wait 2 years for him to turn a nw7 to an nw1? What does that prove? We could have a third-party do a 50-graft test and be done with this who thing. I can't imagine it would cost more than $35,000 usd-- which Arashi may cover almost half of-- and the rest which could maybe be crowdfunded.