The 50 Graft Test Procedure

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  • clarence
    Senior Member
    • Sep 2012
    • 278

    Originally posted by didi
    First, he was thought HASCI wont be able to find 50 usable grafts? How accurate was that
    Oh he really did, didid he, didi?



    Originally posted by 534623
    Of course there are, in general, still losts of hairs/grafts in your donor area


    but the "tricky environment" itself could be somewhat problematic concerning

    - the influence on the regeneration rate of HST extractions;
    - rather problematic to observe/monitor/document the extractions sites,

    IF Kristel is forced to extract the grafts everywhere in a bigger area, instead just within a rather small 2-3 cm² area.

    Comment

    • Arashi
      Senior Member
      • Aug 2012
      • 3888

      I've been thinking about it and honestly I expect only 1 answer from Kristel. Something along the lines of "ALL hairs without a bulb in the petridish will grow back and 80% of the hairs WITH bulb will grow back as well". That's pretty much what they always claimed. Yeah ok, they were talking about grafts instead of hairs but I just can't believe they're going to try to hide behind that.

      We'll see, but the more I think about it, the more I figure it has to be something like this.

      Comment

      • Arashi
        Senior Member
        • Aug 2012
        • 3888

        Originally posted by hellouser
        Um, I'd say 50 would be on the low end. You don't take statistics based on low numbers.

        The 250 grafts is great, gives us a much better picture.
        Exactly. 250 hair is statistically much more significant than just 50 hairs. Variance in 50 hairs is a lot higher.

        Comment

        • Arashi
          Senior Member
          • Aug 2012
          • 3888

          Originally posted by gc83uk
          lol, to be honest I reckon Iron Man thinks we are all idiots who doesn't know anything and he is the only normal person here.
          Haha Actually I'm SURE he thinks that

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          • gc83uk
            Senior Member
            • Nov 2011
            • 1339

            I wonder what Dr Nigam thinks about all of this?

            Comment

            • Arashi
              Senior Member
              • Aug 2012
              • 3888

              Originally posted by gc83uk
              I wonder what Dr Nigam thinks about all of this?
              Haha seriously ? After that picture of NSN he posted with all those circles he made up ? He obviousy has no interest in the truth, just like Didi.

              Comment

              • JJJJrS
                Senior Member
                • Apr 2012
                • 638

                Originally posted by Arashi
                Anyway, good news is she said that they've been taking their own pictures as well, which are still to follow ! So we'll hopefully get better pictures for analysis.
                Just to be clear, the pictures that james posted weren't from HASCI?

                Hopefully they took some nice, high quality photos because that would make the analysis much easier.

                The petri dish photo is great though and certainly very informative.

                Comment

                • JJJJrS
                  Senior Member
                  • Apr 2012
                  • 638

                  Here's my take on everything up to this point:

                  - I haven't counted the number of hairs in the '1' subsection yet. For this post, I'll assume that there's around 400-450 total hairs in the petri dish like Arashi said. I'll verify this later though to make sure.

                  - According to HASCI, they extracted 150 usable singles and 50 usable doubles. If we accept this as true, this implies that there are 250 usable hairs in total. To confirm this, perhaps we can count the number of hairs with intact bulbs in the petri.

                  - If there are 400-450 total hairs and 250 of those hairs are usable, this implies that the other 150-200 hairs are either transections or in telogen. Based on the numbers, it's clear that the majority of these must be transections. These transections should hopefully regenerate in the donor but are unlikely to yield in the recipient. For the telogen hairs, these are unlikely to regenerate in the donor. It's unsure if they will yield in the recipient either.

                  - When calculating the donor regeneration, we should reference this with the 400-450 extracted. When calculating the recipient yield, we should reference this with the 250 usable grafts.

                  - The most important thing we should look at is the net gain in hairs. We started out with 400-450 in the petri, let's see how many we end up with when we count the hairs in the donor and recipient later. This is the simplest proof of concept.

                  Comment

                  • Arashi
                    Senior Member
                    • Aug 2012
                    • 3888

                    Originally posted by JJJJrS
                    - According to HASCI, they extracted 150 usable singles and 50 usable doubles. If we accept this as true, this implies that there are 250 usable hairs in total. To confirm this, perhaps we can count the number of hairs with intact bulbs in the petri.
                    Haven't counted but at first glance I'm pretty sure this will turn out to be true.

                    About the HASCI made photo's: Kristel said in Dutch they've made "mis oecologische" recordings, which will be released.

                    Not sure what "mis oecologische"even means, but oecologische would probably translate as "ecologic" ? Maybe someone knows what she's saying here ?

                    Anyway, I'm sure we'll get an answer from her tomorrow regarding the bulb-less hairs 'mystery'. Like said my guess is that she'll say that ALL bulb-less hairs will regrow and 80% of the hairs with bulb will regrow too. I'd be highly surprised if she doesn't say that.

                    Comment

                    • Arashi
                      Senior Member
                      • Aug 2012
                      • 3888

                      Originally posted by JJJJrS
                      - If there are 400-450 total hairs
                      I'm pretty sure I've overcounted and am going to say there are 390-420 hairs in the petridish. Need some time to really sit down for it but I think this will turn out to be true.

                      Comment

                      • JJJJrS
                        Senior Member
                        • Apr 2012
                        • 638

                        Originally posted by Arashi
                        Anyway, I'm sure we'll get an answer from her tomorrow regarding the bulb-less hairs 'mystery'. Like said my guess is that she'll say that ALL bulb-less hairs will regrow and 80% of the hairs with bulb will regrow too. I'd be highly surprised if she doesn't say that.
                        I totally agree with that and would also be shocked if she doesn't say the same.

                        The majority of these bulb-less hairs must be transections. I'm certain of that and no other explanation makes sense.

                        The nice thing is, we'll be able to see if this explanation is correct in a couple of weeks when the donor starts to regenerate.

                        I wish they had kept it at 50 grafts though. Counting 400-450 hairs won't be fun at all

                        Comment

                        • Arashi
                          Senior Member
                          • Aug 2012
                          • 3888

                          Originally posted by JJJJrS
                          The majority of these bulb-less hairs must be transections.
                          For sure. Anyway, ttyl, hopefully with some good news from her

                          Comment

                          • JJJJrS
                            Senior Member
                            • Apr 2012
                            • 638

                            Originally posted by Arashi
                            I'm pretty sure I've overcounted and am going to say there are 390-420 hairs in the petridish. Need some time to really sit down for it but I think this will turn out to be true.
                            I'll confirm it eventually to make sure. But my impression is that most people will have a tendency to undercount the results.

                            Comment

                            • FearTheLoss
                              Senior Member
                              • Dec 2012
                              • 1581

                              I feel like this test we did won't prove much to anyone..the critics will still be critics because we are still guesstimating things..

                              Comment

                              • didi
                                Senior Member
                                • Nov 2011
                                • 1360

                                arashi

                                can you elaborate when you say all bulbless hairs an hairs will bulbs will regrow...

                                do you mean 2 hair graft will regrow in donor and recipient (real doubling)or 1 hair(bulb) will grow in donor and other bulb-less in recipient or something like that ?

                                Comment

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