I think I've hacked it

[FGF11]

There are a lot of big problems in this world, that hair loss, sometimes seems actually quite small compared to them. Watch The Boy Whose Skin Fell Off, and My Flesh and Blood just to get an idea of how a disease such as Epidermolysis bullosa can be soooooooooo much worse than hair loss. So please stay positive. and be grateful for what you are and have in life but also don't sit on your butt

I'm taking a real break from this for maybe a month or two. I need some refreshing.

[FGF11]

Yes, I'm aware about them.

The first cell therapies for skin was actually developed by Howard Green. He, unfortunately, died. But what he did was, he cultured epithelial human cells in a very specific way. In which, he added the media from under, and layers of epithelia grew out and then he developed autologous cell transplantation. This gave rise to few companies, one of them was named Organogenesis, which developed substitute skins but the company went bankrupt because first. Many of the famous skin biologists today are trained in his lab, including elaine fuchs, which she has trained many others including Dr. Rendl, Dr. Greco, ..

In response to a question, he says the following:

"It's worth noting that at the time we did the initial skin graft experiments, the US Food and Drug Administration (FDA) existed, but they were not interested, and there were no committees whose approval you had to seek. We went ahead with this without any impediments of that sort. Within a few years, I could not do an experiment on a mouse without committee approval, so that's how the situation changed within a short period of time. If I had needed to face that at the beginning, I wouldn't have started the whole thing because it's just too much to deal with."

So even prominent scientist are actually happened by FDA in many ways.

Colin Jahoda, was among the first scientist, who showed that dermal papillae can induce hair follicle regeneration. At the time, he was working at Dr. Reynolds lab, which was a pioneer in hair follicle studies. He has also trained many of Many other scientists, such as Dr. Christiano, which she has Trained Dr. Higgins, and so on. Dr. Jahoda, first transplanted a hair follicle to his wife's hand and showed that dermal papillae can induce hair follicles.

However, later on works from Dr. Mc Elwee showed that derma sheath cells are more important than dermal papillae cells. Dermal papillae cells never actually divide in vivo, so they must come from somewhere. After recent studies, By Dr. Bernieskie, (was trained in Dr. Freda miller lab) he showed that actually Dermal sheath cells contribute to dermal papillae, and dermal papillae is not the important one.


So replicel seems to be on the right track here. Since aderans and intercytex were trying to use dermal papillae (as what Dr. Christiano and Higgins) are trying to do. However replicel is culturing dermal sheath cells.

However, guaranteed it won't work.

Why? you may ask?

Hematopoietic stem cells have been known for 50 years or so, and tons of more research is being done on them for bone marrow transplantation studies. We do have Hematopoietic stem cell for quite a decade now, however, despite 10X more knowledge on them than hair follicle stem cells. Scientists have not yet been able to culture these stem cells. It's not possible. Replicell is on the right track, but it'll take 50 more years for them to learn how to culture these cells. They will differentiate so fast and irreversibly since they are out of their niche.

If some one finds a way to culture Hematopoietic stem cells, he could save millions of people every year. But alas it's not yet feasible. So is hair follicle stem cell culture.


As for cell coating, those are jokes. They are not useful in any sense. The only real use I see, is to some how use them to coast stem cells from other people, or may be embryonic stem cells used by histogen (so these cells would become immune protective) and transplant them back to the hair. So they will secrete growth factors in the head, while the body won't attack them.


This is not something fancy, as viacyte is doing the exact same thing, they're using some one else's embryonic stem cells but they put it in a protective sheet and implant it in the body. They're using it to cure diabetes. They are in clinical trials. As for diabetes, no one has still find a way to replicate those stem cells as well. and we know tons more about beta-cells than any other cells.

So my take, it won't happens soon. Dr. Paul kemp was the smartest and given up on that long ago.

A simple gene analysis will take years if I want to do, and so no I can't do that. Also, I know, even if I try I would fail to keep inductivity of dermal papillae or dermal sheath cells, It's gigantic task.
It's nothing short of miracle.

[joachim]

hey FGF11, i was wondering: do you have the tools and are you able to analyze dermal papilla cells in terms of gene expression? if you are aiming at full regrowth, does this involve some culturing (multiplication) of cells?
i'm not sure if you are aware of all the culturing attempts in the past as you are probably not long enough on the forum.
researchers like jahoda, christiano, and other labs tried to multiply DP cells but none of them succeeded as it turned out the cells lose inductive capabilities and gene expression levels of some genes.
some recent papers seem to indicate that there was progress regarding this but there's no clear answer to that yet. maybe you've seen the other recent thread about the nanocoating which seem to allow the cells to expand and retaining all the necessary properties. however, there is no exact comparison of before/after gene expression levels so this may still be not a full success. i don't know.

are you aware of those issues? does your approach even need some culturing of cells?

[FGF11]

the difference is: this guy tests his theories directly and immediately on himself, because he knows what is dangerous and what not. he knows the risk and he has the know-how to test different things in a safe way on himself.
he doesn't have to do years of tests on mice and go through clinical trials and first find investors who give him millions of dollar to test a theory.
if he knows the science and has access to tools and materials, which all seems to be the case, then he could get very far if he is eager and passionate enough. with the right effort, a single person can get much further than all those so-called hair researchers who spend years and millions of money without real progress. most of them have a full head of hair, so they are not in a hurry to find an effective cure. just tinkering in their labs, year after year, publishing meaningless papers and tests on mice which never work on humans.

[joachim]

The low hanging fruit for cures and treatments is long gone. No one is going to scribble down the cure for MPB on the back of a napkin somewhere its way too hard for that. No one guy is going to hack together the cure for MPB.
A lot of things that in theory could or should work dont in practice ever amount to much if anything. Putting theory into a practical compound that works is really where the rubber meets the road. Easier said than done. Only 2 companies have ever done it.

the difference is: this guy tests his theories directly and immediately on himself, because he knows what is dangerous and what not. he knows the risk and he has the know-how to test different things in a safe way on himself.
he doesn't have to do years of tests on mice and go through clinical trials and first find investors who give him millions of dollar to test a theory.
if he knows the science and has access to tools and materials, which all seems to be the case, then he could get very far if he is eager and passionate enough. with the right effort, a single person can get much further than all those so-called hair researchers who spend years and millions of money without real progress. most of them have a full head of hair, so they are not in a hurry to find an effective cure. just tinkering in their labs, year after year, publishing meaningless papers and tests on mice which never work on humans.

[FGF11]

-

[burtandernie]

The low hanging fruit for cures and treatments is long gone. No one is going to scribble down the cure for MPB on the back of a napkin somewhere its way too hard for that. No one guy is going to hack together the cure for MPB.
A lot of things that in theory could or should work dont in practice ever amount to much if anything. Putting theory into a practical compound that works is really where the rubber meets the road. Easier said than done. Only 2 companies have ever done it.

[jjo]

Hey Fgf I'll tell you what I tell everyone who comes along with a potential new treatment: try it out but give us proof. Pictures, results, numbers comparions with existing treatments and placebo. I hope it works, I wish you the best of luck but the burden of proof is on you.

This is the same I said about Follicept. I am glad they tried but they haven't proven it works. However that just means we need more people trying.

leave him alone and stop asking for pics and proof...the guys working on it and obviously wants this. he hasn't made any promises i'm sure when he has something to report he will, pics will come later.

thanks FGF11 for all your time and effort.

[Westonci]

Hey FGF11 why don't you hire some interns man? Sounds like you're pretty swamped. Hopefully you can get a whole operation off the ground, there are a lot of people on these forums that could probably lend a hand in some way, at least on the non-scientific aspect of things. I do think that the only real solution to baldness will come through genetic manipulation. I'm pretty surprised that others are not trying to tackle it from this angle. I wish you all the luck. I'm in contact with Dr. Hsu from the follicept team,he's a very capable scientists, and although I don't think that their approach to hairloss is going to work at all, he might be open to collaborating or helping you find resources if he's swayed by your science. I can't recommend him enough as a person.

Hey FGF11, I live in Toronto. If you live here, I can volunteer my time to help your cause. Will do anything to help you.

[Hemo]

Good luck friend

[InBeforeTheCure]

But AR is not the only thing that I am playing with, and so I'm going to actually go for complete reversal. But I won't be talking about that. Because I will only confuse you guys, but don't expect anything soon.

Pls tell. We can deal with confusion.

Unless of course you're concerned about others stealing your ideas. Then it's best not to say, of course.

[Hairmore]

Fgf, perhaps you can convince some chemist or group of scientist of your idea and you could test it somehow together. I would love to know if it really works. I am also not convinced without any images.

[Sogeking]

Hey Fgf I'll tell you what I tell everyone who comes along with a potential new treatment: try it out but give us proof. Pictures, results, numbers comparions with existing treatments and placebo. I hope it works, I wish you the best of luck but the burden of proof is on you.

This is the same I said about Follicept. I am glad they tried but they haven't proven it works. However that just means we need more people trying.

[Sogeking]

double.

[FGF11]

By all means continue with your project and prove us wrong. Until then anyone with a brain would be somewhat skeptical.

I, myself, am skeptical as well.

actually everyone must be very skeptical.

I have not started anything yet (the actual self trial), so there is no reason to lie.

On paper though, and based on what I know from a small experiment, I have my hopes high and actually my fingers crossed.