Electric stimuli induces mechanisms activation of Wnt/β-catenin and MAPK pathway

[noisette]

Hair growth promoting effects of different alternating‐current parameter settings are mediated by the activation of Wnt/β‐catenin and MAPK pathway

Abstract

Electrical stimulation is being used in variable skin therapeutic conditions. There have been clinical studies demonstrating the positive effect of electrical stimuli on hair regrowth. However, the underlying exact mechanism and optimal parameter settings are not clarified yet. To investigate the effects of different parameter settings of electrical stimuli on hair growth by examining changes in human dermal papilla cells (hDPCs) in vitro and by observing molecular changes in animal tissue. In vitro, cultured hDPCs were electrically stimulated with different parameter settings at alternating current (AC). Cell proliferation was measured by MTT assay. The Ki67 expression was measured by immunofluorescence. Hair growth-related gene expressions were measured by RT-PCR. In animal model, different parameter settings of AC were applied to the shaved dorsal skin of rabbit for 8 weeks. Expression of hair-related genes in the skin of rabbit was examined by RT-PCR. At low voltage power (3.5 V) and low frequency (1 or 2 MHz) with AC, in vitro proliferation of hDPCs was successfully induced. A significant increase in Wnt/β-catenin, Ki67, p-ERK and p-AKT expressions was observed under the aforementioned settings. In animal model, hair regrowth was observed in the entire stimulated areas under individual conditions. Expression of hair-related genes in the skin significantly increased on the 6th week of treatment. There are optimal conditions for electrical stimulated hair growth, and they might be different in the cells, animals and human tissues. Electrical stimuli induce mechanisms such as the activation of Wnt/β-catenin and MAPK pathway in hair follicles.

[NeedHairASAP]

Hair growth promoting effects of different alternating‐current parameter settings are mediated by the activation of Wnt/β‐catenin and MAPK pathway

Abstract

Electrical stimulation is being used in variable skin therapeutic conditions. There have been clinical studies demonstrating the positive effect of electrical stimuli on hair regrowth. However, the underlying exact mechanism and optimal parameter settings are not clarified yet. To investigate the effects of different parameter settings of electrical stimuli on hair growth by examining changes in human dermal papilla cells (hDPCs) in vitro and by observing molecular changes in animal tissue. In vitro, cultured hDPCs were electrically stimulated with different parameter settings at alternating current (AC). Cell proliferation was measured by MTT assay. The Ki67 expression was measured by immunofluorescence. Hair growth-related gene expressions were measured by RT-PCR. In animal model, different parameter settings of AC were applied to the shaved dorsal skin of rabbit for 8 weeks. Expression of hair-related genes in the skin of rabbit was examined by RT-PCR. At low voltage power (3.5 V) and low frequency (1 or 2 MHz) with AC, in vitro proliferation of hDPCs was successfully induced. A significant increase in Wnt/β-catenin, Ki67, p-ERK and p-AKT expressions was observed under the aforementioned settings. In animal model, hair regrowth was observed in the entire stimulated areas under individual conditions. Expression of hair-related genes in the skin significantly increased on the 6th week of treatment. There are optimal conditions for electrical stimulated hair growth, and they might be different in the cells, animals and human tissues. Electrical stimuli induce mechanisms such as the activation of Wnt/β-catenin and MAPK pathway in hair follicles.

hint hint: pilox

[joachim]

hint hint: pilox

this is not true actually. pilox does not do exactly this electrical stimulation with that frequency.

however, as pilox already has the right electrodes to do the iontophoresis-like thing with the copper and zinc, it would be perfectly suited for an upgrade to test that theory. creating an AC source below 5V and at a few MHz frequency is not really that hard. maybe we should pitch that idea to the pilox creators.
i still don't believe in the effectiveness of pilox for now, but if they could add that AC source which could stimulate the cells in a positive way, this could be really huge.
i always had the feeling that some kind of electrical stimulation at certain frequencies could be beneficial to keep cells alive and profilating, but it's so hard to try all different combinations. thus, it's very interesting to see a study about exactly this. maybe it's not a full cure, but it could be highly beneficial if we could put the sleeping cells into an active state again.

needhairasap, are you in contact with the pilox guys? could you reach out to them and forward them the study and the idea to integrate it into the pilox device?