Increasing the hair inductive potential of DP cells

[lacazette]

SARI, AGNES ROSARINA PRITA is a researcher from melbourne, and here it's her master research thesis:


Increasing the hair inductive potential of human dermal papilla cells: stimulating and characterising cell aggregation

https://minerva-access.unimelb.edu.au/handle/11343/55427

Dermal papilla cells (DPCs) are able to induce hair follicles. DPC tend to aggregate both in vitro and in vivo. This tendency is associated with their ability to induce hair growth. The use of DPCs to treat alopecia is limited because human DPCs lose their hair-inducing activity in culture, whereas ovine DPC do not.

The aims of this study were to characterise the molecular phenotype of ovine DPC aggregates, and to determine whether aggregating ovine DPCs secrete factors affecting the aggregative behaviour or inductive potential of human DPCs.

Expression of papilla markers in cultured ovine DPCs was characterised. The effects of ovine factors, different culture substrates and medium compositions on aggregative behaviour of human DPCs were determined. Co-cultures of ovine and human papilla cells, separated by a permeable membrane were observed to determine whether the ovine cells secrete soluble factors that affect human cell aggregation.

Ovine DPC aggregates expressed 16 papilla markers, showing they have a similar phenotype to papillae in vivo. In co-culture experiments, well-formed aggregates were produced in humanvine DPC mixtures. In contrast, unmixed human DPCs remained in a monolayer state, indicating that ovine cells are required to initiate aggregation but the human cells are then able to incorporate into aggregates. Both human and ovine DPCs had a higher tendency to aggregate in medium containing 20% (v/v) lamb serum compared to 10% (v/v) foetal calf serum. The effect of co-culturing human with ovine DPCs separated by a permeable membrane gave positive additional effects to human aggregation.

In summary, ovine biomolecules show potential for increasing the aggregative behaviour of human DPCs in culture. These biomolecules might eventually be used to treat androgenetic alopecia.
"


So apparently her biovine molecules could help to culture inductive DP cells for a cellular therapy, but could also eventually be used directly as a medication for existing hair

I saw also that she was one of the travel grant recipients from the 2014 World Congress Hair Research

http://www.hair2014.org/Contents.asp?midx=14&openpage=14

Her work seems quite interesting but I don't find her contact info, would like to ask what she is doin now, if she had link with a company or what,etc.. so if someone find it

[nameless]

This is interesting but they can't inject ovine cells into humans because doing so would trigger an immune response.

[noisette]

Hi bro,
I found her facebook lol : https://www.facebook.com/agnes.pritasari

[JayM]

Get in her DM's son.

[JayM]

She is the media and communications officer on whatever she does anyway. So could be a good shout getting a reply

[baldybald]

Now, do we need to understand the hair loss mechanism to find the cure ?

[Vic]

Now, do we need to understand the hair loss mechanism to find the cure ?

Can't solve the problem without knowing what the problem is

[lacazette]

@nameless, hey dude it'q not injecting ovine cells but as it is saying : co-culturing human with ovine DPCs separated by a permeable membrane ( to keep the inductive potential

@noisette, thanks bro I sent her a msg, though it will go in her 'other' category of msgs, Im afraid she will not see it

[lacazette]

In this study from the Us army researchers, thampagazam and darling, in 2014 , they explained where we were about a DPs therapy:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057739/

Cells with the potential for hair follicle neogenesis can be either injected directly in the bald scalp or in areas where hair follicles are desired or can be incorporated in skin substitutes to regenerate skin appendages. One limitation of applying these cells for hair regeneration is that expansion and frequent passage of trichogenic cells results in loss of hair inductive potential. Advancements in cell culture technique like using less trypsin during multiplication [106], three dimensional culture systems [107,108], xeno free culture system [109], conditioning with human serum [63,110] or supplementing media with morphogens like wnt and bmp modulators [111–113] can lead to methods for extending the efficacy of these cells.

We used the reconstitution assay [114,115] with minor modifications to evaluate whether the trichogenic potential of DP cells can be maintained by using three dimensional culture systems.
The resultant HFs contained hair shafts and sebaceous glands (Figure 4A,B), similar to the results of others
We have also recently demonstrated for the first time that isolated, cultured and dissociated human DP cells can induce complete human hair follicles when combined with neonatal foreskin keratinocytes and grafted onto a nude mice [116].


Cellular therapy based on autologous dermal fibroblast holds enormous promise to the field of regeneration medicine. It offers a safe, immunologically acceptable and simple alternative for tissue regeneration applications.
Practical challenges including scale up, formulation, storage, transport and delivery mechanisms are the main challenges to development and clinical translation of preclinical observations. However, recent FDA approval of fibroblast therapy applications for nasolabial folds and the expanding pipeline of other fibroblast therapies in clinical development show that cellular therapy is an expanding field.


scale up, formulation, storage, transport and delivery mechanisms" there are tremendous progress in each kind of theses challenges nowadays. We are more in a technology.practical waiting game for us now, more than a knowledge wiating game about DP indution,etc

[Keeper]

Invite her to the forum, maybe she can discuss some questions

[nameless]

@nameless, hey dude it'q not injecting ovine cells but as it is saying : co-culturing human with ovine DPCs separated by a permeable membrane ( to keep the inductive potential

@noisette, thanks bro I sent her a msg, though it will go in her 'other' category of msgs, Im afraid she will not see it

lacazette, thanks for clearing that up. Sometimes this science can be hard to absorb. So if I'm to understand this correctly doesn't this mean that they culture the human cells in the same container as the ovine cells but they keep the ovine cells and human cells separated in that container? And if this is the case doesn't this mean that after they culture the bovine cells and human cells they can simply remove the ovine cells and save the human cells.

[lacazette]

yes it's exactly that Nameless. Apparently ovine cells secrete a combination of factors that make them don't lose their hair inductive potential. So co culturing ovine and human DPs even seperate by a membrane, make the human DPs benefit from these factors aswell and increase their hair induction

[lacazette]

There's also this interesting girl, if someone find her mail

She is working on hair regeneration and artificial dermal papilla, and had a presentation at the last ISMS 2015 (international symposium on morphological sciences)

http://www.isms2015.org/cv/chengminlin.pdf

Dr. Chang-Min Lin,PhD and Associate professor, is the Director of English Curriculum and Foundation Study Block in English Curriculum, Department of Histology and Embryology, Shantou University Medical College, China.
She has studied the "Regeneration of Hair Follicle", obtained 3 patents for the devices of Hair Follicle transplantation,got 4 items of research grants.

Title: Comparison of Calcium and Barium Microcapsules as Scaffolds in the Development of Artificial Dermal Papillae

[lacazette]

Also dp related, there is this lab from Cornell Univ engineering:

The Organic Robotics Lab (ORL) focuses on using synthetic adaptation of natural physiology to improve machine function and autonomy. Our research spans three primary areas: bioinspired robotics, soft sensors and displays, and advanced manufacturing. We use soft materials, mechanical design, and novel fabrication methods to replicate sensory organs such as dermal papillae, replicate organs that rely on actuation such as the heart, and to power soft actuators and robots.

https://orl.mae.cornell.edu/#about