Who is this guy? 'Hair follicles made ex vivo that can be inserted..'

[Desmond84]

Hi Desmond. When ready will their treatment be via injection? Or growing hair follicles in a lab & transplanting into scalp?

Tbh Renee, the answer revolves around how soon they can expand human DP cells in a lab. The latest theory is human DP cells lose their trichogenicity (hair inducing properties) when they leave the ECM (extra-cellular matrix) which is a fluid they are bathing in at the hair shaft. When we extract the DP cells from the hair shaft to expand them in the laboratory they lose all their hair inducing properties. We need to find a way to produce this soup of chemicals making up the ECM and then bathing the DP cells inside it on a 3D scaffold. This will provide us with viable DP cells which will be replicating themselves. Once we can produce millions of DP cells with 100% trichogenicity, we simply place them on top of Keratinocytes and voila hairs will be growing in a matter of days. The hardest part has been finding all the chemicals making up the ECM and scientists may crack it at any point.

Tsuji and many others have shown that if you have trichogenic DP cells, the rest of hair regeneration is pretty much automatic. Look up the hair germ method as an example. We are stuck on this one final step. And 2 teams at the congress assured me last year we are merely 1 or 2 years away from solving this problem.

[JayM]

102 years? I best get on some supplements But that's a really great way to have it explained!

[Desmond84]

Of course once they are able to do that, it needs to undergo clinical trials for 5-7 years before it becomes commercially available to public.

[Renee]

Thank you for your prompt informative response. I found the below from pubmed.gov published on June 29,2015. Your thoughts?

Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system.
Dong L1, Hao H1, Liu J1, Tong C1, Ti D1, Chen D1, Chen L1, Li M2, Liu H1, Fu X1, Han W1.
Author information

Abstract
Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study. Wnt-CM was prepared from the supernatant of Wnt1a-expressing bone marrow mesenchymal stem cells (BM-MSCs) that maintain the hair-inducing gene expression of DP cells. The collagen-chitosan scaffold cells (CCS cells) were constructed using a two-step method by inoculating the Wnt-CM-treated DP cells and epidermal (EP) cells into the CCS. The cells in the air-liquid culture formed dermal condensates and a proliferative cell layer in vitro. The CCS cells were able to induce hair regeneration in nude mice. The results demonstrate that Wnt-CM can maintain the hair induction ability of DP cells in expansion cultures, and this approach can be used for large-scale preparation of CCS cells in vitro to treat hair loss. Copyright © 2015 John Wiley & Sons, Ltd.
Copyright © 2015 John Wiley & Sons, Ltd.

[Desmond84]

102 years? I best get on some supplements But that's a really great way to have it explained!

Hahaha sorry that was a typo :P I just fixed it

[lacazette]

Regarding 3D printing, here is a canadian team who is in the process of commercialized a 3D printer machine that generate hair follicles

http://www.computerworld.com/article/2688824/3d-printed-skin-holds-promise-for-burn-victims-and-others.html

"A 3D printer that can use a patient's own cells to print skin grafts, including hair follicles and sweat glands, has won a scientific design competition.

While still in pilot mode, the PrintAlive Bioprinter is in the process of being commercialized by MaRS Innovations in collaboration with the Innovations and Partnerships Office (IPO) of the University of Toronto, whose labs have filed two patents on the device.

Four University of Toronto engineers created the printer, which is about the size of a standard microwave oven. Last week, the machine won the James Dyson Award for student design, setting the stage for the group to compete for a $60,000 international prize.

Since 2008, the team has developed hundreds of design iterations to optimize how the machine operates. Recently completing a second generation, pre-commercial prototype of the machine, they hope to scale up their device from its current bench-top process to a higher volume automated process.

"But until now no one has demonstrated a simple and scalable one-step process to go from microns to centimeters."

Along with producing skin cells, the machine can also produce hair follicles, sweat glands and other human skin complexities, "providing an on-demand skin graft for burn victims," the university stated.
The university researchers are working with a burn unit at Toronto's Sunnybrook Hospital.


The University of Toronto is not alone in using 3D printing technology to create human tissue. Other university researchers and private companies are developing printing technology that could someday be used to replace organs and other body parts.

San Diego-based bio-printing company Organovo expects to unveil the world's first printed organ -- a human liver -- this year.

The Wyss Institute for Biologically Inspired Engineering at Harvard University has created a 3D printer that can lay down four different types of cells at the same time. The breakthrough in that research has been the ability to create blood vessels that can feed living tissue.

[Desmond84]

Thank you for your prompt informative response. I found the below from pubmed.gov published on June 29,2015. Your thoughts?

Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system.
Dong L1, Hao H1, Liu J1, Tong C1, Ti D1, Chen D1, Chen L1, Li M2, Liu H1, Fu X1, Han W1.
Author information

Abstract
Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study. Wnt-CM was prepared from the supernatant of Wnt1a-expressing bone marrow mesenchymal stem cells (BM-MSCs) that maintain the hair-inducing gene expression of DP cells. The collagen-chitosan scaffold cells (CCS cells) were constructed using a two-step method by inoculating the Wnt-CM-treated DP cells and epidermal (EP) cells into the CCS. The cells in the air-liquid culture formed dermal condensates and a proliferative cell layer in vitro. The CCS cells were able to induce hair regeneration in nude mice. The results demonstrate that Wnt-CM can maintain the hair induction ability of DP cells in expansion cultures, and this approach can be used for large-scale preparation of CCS cells in vitro to treat hair loss. Copyright © 2015 John Wiley & Sons, Ltd.
Copyright © 2015 John Wiley & Sons, Ltd.

No problems Renee it's my pleasure. The Wnt's role has been well-studied and most researchers are well aware of its importance. Actually, Dr Aaron Gartner came on BTT last year and mentioned he'll be testing Wnt-1a on DP cells in their hanging-drop model to see what would happen. From what I remember they are actually testing a range of signals and measuring the trichogenicity of DP cells post-treatment. It'll be interesting to see what they have found out over the last 15 months since they presented their findings.

The Wnt/β-catenin signalling are definitely key to DP inductivity. Hopefully Dr Jahoda/CHristiano's team will be presenting again at the Hair Congress this year

[Renee]

Thank you Desmond. Hopefully they crack the code soon, if they haven't already! Thanks.

[Desmond84]

Regarding 3D printing, here is a canadian team who is in the process of commercialized a 3D printer machine that generate hair follicles

http://www.computerworld.com/article/2688824/3d-printed-skin-holds-promise-for-burn-victims-and-others.html

"A 3D printer that can use a patient's own cells to print skin grafts, including hair follicles and sweat glands, has won a scientific design competition.

While still in pilot mode, the PrintAlive Bioprinter is in the process of being commercialized by MaRS Innovations in collaboration with the Innovations and Partnerships Office (IPO) of the University of Toronto, whose labs have filed two patents on the device.

Four University of Toronto engineers created the printer, which is about the size of a standard microwave oven. Last week, the machine won the James Dyson Award for student design, setting the stage for the group to compete for a $60,000 international prize.

Since 2008, the team has developed hundreds of design iterations to optimize how the machine operates. Recently completing a second generation, pre-commercial prototype of the machine, they hope to scale up their device from its current bench-top process to a higher volume automated process.

"But until now no one has demonstrated a simple and scalable one-step process to go from microns to centimeters."

Along with producing skin cells, the machine can also produce hair follicles, sweat glands and other human skin complexities, "providing an on-demand skin graft for burn victims," the university stated.
The university researchers are working with a burn unit at Toronto's Sunnybrook Hospital.


The University of Toronto is not alone in using 3D printing technology to create human tissue. Other university researchers and private companies are developing printing technology that could someday be used to replace organs and other body parts.

San Diego-based bio-printing company Organovo expects to unveil the world's first printed organ -- a human liver -- this year.

The Wyss Institute for Biologically Inspired Engineering at Harvard University has created a 3D printer that can lay down four different types of cells at the same time. The breakthrough in that research has been the ability to create blood vessels that can feed living tissue.

Yeah this 3D printer will come in very handy once we can mass produce DP cells. All of these technologies are complementing each other making organ regeneration a reality

[Desmond84]

Thank you Desmond. Hopefully they crack the code soon, if they haven't already! Thanks.

Hahaha I hope so too. Dr Beren Atac sent me an email saying she's preparing to release a new paper on Lauster's hair regeneration model. I wonder if it's big news or not. Hopefully she gets to present it in Miami in November

[lacazette]

3D printed skin sooner in japan


Japanese Researchers Say Bone and Tissue Bioprinting Process Could Be A Year Away From Use In Humans

http://3dprint.com/37745/bone-and-tissue-bioprinting/

"While groups of researchers around the world have developed small masses of tissue for use as implants, this project aims to take the next step towards making them functional and approved for use in humans. Tsuyoshi Takato, a professor at the University of Tokyo Hospital, says his team has built the next-generation 3D bioprinter which will be used to create custom-fit bone and tissue."

He adds that Japanese medical authorities are expected to grant approval for the process soon, and that the 3D bioprinter will be available commercially during 2015 if Japan’s Pharmaceutical and Medical Devices Agency (PMDA) approves the device and the process.

Takato says clinical tests of 3D printed skin will be followed by tests of materials and processes to build bones, cartilage and joints, but he adds that the main hurdle holding the project back – the heat generated by conventional 3D printers, which damages living cells and protein, is still being studied.

“We haven’t fully worked out how to avoid heat denaturation, but we already have some models and are exploring which offers the most efficient method,” Takato says

Finally, Takato stated that their machine would be ready for clinical trials focussed on human skin within the next three years"

With the new approval design in japan, the "five years away cure" sounds to become a reality. Especially when I read your excellent posts Desmond

[lacazette]

Japan to invest $21 million in developing 3D printed human organs

http://www.3ders.org/articles/20150115-japan-to-invest-21-million-in-developing-3d-printed-human-organs.html

"Could 3D printable human organ transplants become a reality in the coming years? It’s certainly starting to look that way, as reports are surfacing that the Japanese government will also begin heavily investing in this very human branch of 3D bio printing technology.

This sum is to be spread out over five research organizations attached to Osaka University, who will use it to develop 3D printable human tissue and ultimately transplantable organs. Research into human tissue printing is reportedly already well underway in Osaka, where research teams have been working with ips cells (induced pluripotent stem cells, or artificially cultivated stem cells that can be used in 3D bio printing processes)

[Renee]

Stuff like this is too far away. Your best bet now is from the research teams of dr lauster, dr christiano or dr tsuji. Ips cells are dangerous and there needs to be more studies regarding safety. On the other hand the methods in development by the first 2 researchers I mentioned have a good safety profile and some clinical data of using ones own cells that supports safety (i.e. Intercytex, aderans, replicel,etc.)

[lacazette]

There's also L'oreal in the 3D printed skin's game

http://www.wired.com/2015/05/inside-loreals-plan-3-d-print-human-skin/

"The idea is to produce skin more quickly and easily using what is essentially an assembly line developed with Organovo, a San Diego bioprinting company. Such a technique would allow the French cosmetics company to do more accurate testing, but it also has medical applications—particularly in burn care.
3-D-bioprinted skin potentially could be produced faster, provided Organovo can successfully replicate the cell structure of human epidermis.

L’Oreal already has a massive lab in Lyon, France, to produce its patented skin, called Episkin, from incubated skin cells donated by surgery patients.
Organovo pioneered the process of bioprinting human tissues, most notably creating a 3-D-printed liver system. Both parties benefit from the partnership: L’Oreal gets Organovo’s speed and expertise, and Organovo gets funding and access to L’Oreal’s comprehensive knowledge of skin, acquired through many years and over $1 billion in research and development.

In concept, it’s the same idea of programming the 3-D printer to print architecture on an X-Y-Z axis,” he says, referring to the CAD designs that typically inform 3-D printers. “We just happened to use living human cells. There’s delicacy involved.” During the last step, the structure of cells is nourished (Renard won’t say how) and kept in a temperature-controlled environment so they can fuse into a cohesive mass of tissue.

[lacazette]

Another Step Towards 3D Printing Skin Cells
Fri, 07/17/2015

http://www.mdtmag.com/news/2015/07/another-step-towards-3d-printing-skin-cells

German chemical giant BASF signed an agreement with French biotech firm Poietis in hopes of improving skin treatment with the use of 3D printing. Poietis pioneered a method of laser-assisted bioprinting that enables scientists to reproduce organic tissue. The technology will allow BASF to print skin cells in three-dimensional patterns within its Mimeskin skin equivalent material.

there's so much things about 3D bioprinting and especially skin on Google, I stop don't wanna spam the forum ^^

And to come back on the topic, please could someone with a fluent oral English could try to call at jordan Fabriquant offices? to just ask if he can give some informations about what's goin on regarding hairloss and his patent?
We never know, maybe he could answer like "yes i'm currently testing it in a preclinical trial, and it's working as hell son, be prepared to get the David Luiz haircut" haha ok i need to sleep