Differences between In-Vivo and In-Vitro (SHD)!

[drnigams]

Boldy,
No need for apology...
infact you spoke the truth..which if i speak... it would be taken the other way...Believe it or not ..it is the forum which i joined 5 months back..which has put my work in this field on fast track..specially when i realized how much it was effecting peoples life...I still can't believe why the pace of the work in HM and dp culture was so slow...

again,My apology for my past negativity, compared with a normal HT procedure, this is of course outstanding already, since there will be donor regeneration !

Its just my opinion, that cell therapy should be the way to go, instead of manual HT/doubling.

thanks, for your presence on these forums, and the pioneering work.

Looking forward to your better version of HM.

[Boldy]

BOLDY,
For me it is not just about money.
Doubling is only the interim option given to patients ...till... as u correctly said...dp culture plus epithelial injections is available hopefully in coming months ...when we can stop doubling and offer only HAIR MULTIPLICATION.

again,My apology for my past negativity, compared with a normal HT procedure, this is of course outstanding already, since there will be donor regeneration !

Its just my opinion, that cell therapy should be the way to go, instead of manual HT/doubling.

thanks, for your presence on these forums, and the pioneering work.

Looking forward to your better version of HM.

[drnigams]

Ted,
First of all let me thank you for reminding me to check when aderans(stenn),histogen(naughton) presentation were,
Looking at the so many star stemcell researchers present in the international investigative dermatology conference.
I have decided to extend my visit to edinburgh by 11th may instead of 8th may.
This is why i say forum is powerful and great for research and knowledge sharing from across the globe,and i thank desmond also for the same.
We already give injections of autologous stemcellsof epithelial and dermal origin(bulge cd34+,k19 ,outer root sheath,dermal cup sheath,dermal papilla,resident epidermis and dermis stemcells).
But what we were missing was dermal papilla culture ,we started dp culture 15days back..1st culture should be ready in 4 weeks time to be given to the patient,and in coming months we will see in practice does that give similar results regarding follicular regeneration as published in many papers by leading scientists in biotech.
Yes, since 2months we are observing better results with dp cell isolation and injection and growth factors injections with our HM injections.
Interestingly a korean scientist is presenting the results of growth factors injection on hair regeneration and hair density results on human trial done by him.
We will have to go beyond dp culture ..and create a cohesive structure similar to dermal papilla which secretes growth factors and extracellular matrix ,i think for better results with HM injections.
But this will happen in probably next 6months..

Are you working on this? Or who will make these injections available in that short time?

[Arashi]

Why HASCI dont go after Umar, Woods, RAssman..they all said HST is FUE/splitting procedure?

I dont know nor care about these doctors. But i do feel hasci should know what lies he's telling for example regarding those 50 lost grafts

[Arashi]

Really? HAIR MULTIPLICATION?

So you try to offer something ...

http://youtu.be/GL9V448xkfs

... as described in this (very old) video?? lol

At least we now understand his big masterplan. He's going to offer doubling for a few months and just when people are starting to find out it doesnt work he's going to offer something new, something better. Lol

[didi]

Ok, I've sent an email to HASCI with the lies you've been posting Dr Nigams. I sure hope they're going to send their lawyers after you. It's just insane how you keep bashing them with all your lies and twisted truths. I can somewhat understand that a forum member like Didi does that but you as a doctor ?? I really just can't believe it ...

Why HASCI dont go after Umar, Woods, RAssman..they all said HST is FUE/splitting procedure?

[Imalmostbald]

Just like boldy said, its one big fiasco. Just a matter of time before we have it now. Keep us up to date with the dp culture nigams, finally we would have the ultimate cure. And we can step aside from fue (even with high % donor regen.) Keep it up!

[534623]

Doubling is only the interim option given to patients ...till... as u correctly said...dp culture plus epithelial injections is available hopefully in coming months ...when we can stop doubling and offer only HAIR MULTIPLICATION.

Really? HAIR MULTIPLICATION?

So you try to offer something ...

http://youtu.be/GL9V448xkfs

... as described in this (very old) video?? lol

[Ted]

BOLDY,
For me it is not just about money.
Doubling is only the interim option given to patients ...till... as u correctly said...dp culture plus epithelial injections is available hopefully in coming months ...when we can stop doubling and offer only HAIR MULTIPLICATION.

Are you working on this? Or who will make these injections available in that short time?

[Arashi]

Ok, I've sent an email to HASCI with the lies you've been posting Dr Nigams. I sure hope they're going to send their lawyers after you. It's just insane how you keep bashing them with all your lies and twisted truths. I can somewhat understand that a forum member like Didi does that but you as a doctor ?? I really just can't believe it ...

[Boldy]

Dear nigams,

It was not the intention to point to you regarding its about the money, but to all HT clinics in general!. the dp procedure is known for more than 25 years, and until today, everyone is still playing around with old school surgical techniques since it pays well enough.

regarding your current donor saving / doubling procedure, of course its better then a normal HT, lets be clear about that.

[drnigams]

BOLDY,
For me it is not just about money.
Doubling is only the interim option given to patients ...till... as u correctly said...dp culture plus epithelial injections is available hopefully in coming months ...when we can stop doubling and offer only HAIR MULTIPLICATION.

its great that nigams and gho spare the Donor but,


Both are nothing compared to what already is posible in a culture dish, take 10 dermal papilla's, culture them for 10 weeks and you'll get +- 95367431640625 dermal papilla's each able to grow a healthy hair folicle, if placed near epithelial cells, or injected with epithelial cells.



Why go under the knife, if my mentioned method ****ing easy to do. , But I guess its less profitable, since the cells need allot of care and time to culture them right.... HT's are more profitable!, end conclusion, its all about the F**ing money, and not about the best cure.

sorry for my negativity, but its the truth.

I wish you all the best.

Boldy

[Arashi]

I also asked Dr. Nigam about the difference between Dr. Gho's HST In-Vivo and his SHD In-vivo, he answered me the following:-

HST invivo extracts complete follicle with bulb, as can be seen in their Petri dish of 200 grafts sample, Dr. Nigam's In-Vivo does not extracts complete follicle but partial follicle without bulb as per the pics of his partial follicle extracted in Vivo are posted on his thread of NW7 case in TBT.

HST claims that they break the stemcell portion at the donor and because they soak their extracted follicle in preservation medium they claim that they can multiply follice, which he doubts is true.

At Dr. Nigam's, he extracts partial follicle at the level of dermal papilla so that even the partial follicle has some portion of dermal papilla, which has capacity to regenerate into a complete follicle

At Dr. Nigam's, he has access to progenitor stemcells from lab,growth factors to boost the partial follicle growth.

Also why he modified the technique to In-Vivo from In-Vitro is to avoid total extraction of graft. They both have their role depends on case to case

However both great techniques of Gho's HST and Nigam's SHD are scarless. There is no wastage of grafts with Dr. Nigam's SHD..as you can see with HST 200 grafts test they had to throw away 50 grafts.

HST claims donor regeneration of 80% while Dr. Nigam's claims regeneration of 100%. However recipient regeneration claim by both is same. Also the price is cheaper due to the location probably.

Finally unfortunately Dr. Umar, Dr. Rassman and Dr Rahal doubt HST saying that they extract one follicle out of 2 follicles and implant at the recipient.

After all I respect both surgeons Dr. Gho and Dr. Nigam and believe that they are trying hard to bring FUE and Hair doubling to another level. Im just a messenger guys so take it easy on me.

Ok this is too much for me. I'm going to forward this to HASCI and show them how Dr Nigams is trying to damage their good name with these countless and blunt lies.

[Arashi]

please delete this

[Boldy]

I also asked Dr. Nigam about the difference between Dr. Gho's HST In-Vivo and his SHD In-vivo, he answered me the following:-

HST invivo extracts complete follicle with bulb, as can be seen in their Petri dish of 200 grafts sample, Dr. Nigam's In-Vivo does not extracts complete follicle but partial follicle without bulb as per the pics of his partial follicle extracted in Vivo are posted on his thread of NW7 case in TBT.

HST claims that they break the stemcell portion at the donor and because they soak their extracted follicle in preservation medium they claim that they can multiply follice, which he doubts is true.

At Dr. Nigam's, he extracts partial follicle at the level of dermal papilla so that even the partial follicle has some portion of dermal papilla, which has capacity to regenerate into a complete follicle

At Dr. Nigam's, he has access to progenitor stemcells from lab,growth factors to boost the partial follicle growth.

Also why he modified the technique to In-Vivo from In-Vitro is to avoid total extraction of graft. They both have their role depends on case to case

However both great techniques of Gho's HST and Nigam's SHD are scarless. There is no wastage of grafts with Dr. Nigam's SHD..as you can see with HST 200 grafts test they had to throw away 50 grafts.

HST claims donor regeneration of 80% while Dr. Nigam's claims regeneration of 100%. However recipient regeneration claim by both is same. Also the price is cheaper due to the location probably.

Finally unfortunately Dr. Umar, Dr. Rassman and Dr Rahal doubt HST saying that they extract one follicle out of 2 follicles and implant at the recipient.

After all I respect both surgeons Dr. Gho and Dr. Nigam and believe that they are trying hard to bring FUE and Hair doubling to another level. Im just a messenger guys so take it easy on me.

its great that nigams and gho spare the Donor but,


Both are nothing compared to what already is posible in a culture dish, take 10 dermal papilla's, culture them for 10 weeks and you'll get +- 95367431640625 dermal papilla's each able to grow a healthy hair folicle, if placed near epithelial cells, or injected with epithelial cells.



Why go under the knife, if my mentioned method ****ing easy to do. , But I guess its less profitable, since the cells need allot of care and time to culture them right.... HT's are more profitable!, end conclusion, its all about the F**ing money, and not about the best cure.

sorry for my negativity, but its the truth.

I wish you all the best.

Boldy