A case of NW7 for in-vivo Doubling / HM / DP - Day 1 @ Dr. Nigam's

[didi]

Dr Nigam

Get interviewd by Spencer, I know you tried it before and it failed but Spence keeps talking about you in his radio shows which is a good sign


I wish you got into HM 10 years ago, by now we'd have cure

[534623]

7)The claim that stemcells are extracted and implanted after soaking in preservation medium to multiply follicle stemcells is ultimately proved as an ILLUSION.

Excuse me, but where is the "ultimate proof" of this "ILLUSION" you're TALKING about?
Where is your proof for your incompetent and fraudulent claims?

But let me explain you (again) how stupid and incompetent you are...

EVERYBODY out there - even laymen! - know, that if you pluck, I mean, if your really PLUCK out an anagen hair with out fingers or tweezers or similar "tools" - it grows back...


So what part of this don't you understand?

Furthermore, researchers out there know since DECADES, that follicular stem cells are still attached to PLUCKED hairs...

The scientific evidence for that is simply there since DECADES ...

http://www.ncbi.nlm.nih.gov/pubmed/15149497

Anyway, if there are follicular stem cells attached to the plucked hairs - where do these MISSING follicular stem cells come from, when the hair follicle completely regenerates itself and its hair shaft grows back??

WHERE DO THE MISSING FOLLICULAR STEM CELLS (due to hair plucking) COME FROM??
Definitely not from Dr. Nigams "FDA certificated stem cells Lab"...

So what part of this don't you understand?

Completely the same for the recipient area,
IF you try to IMPLANT plucked hairs:

A portion of the hair follicle stem cells are attached to the plucked hairs, and if this plucked hair regenerates COMPLETELY also in the recipient area - WHERE THE HELL DO THE MISSING FOLLICULAR STEM CELLS COME FROM??

In simple words:
What exactly multiplies the missing follicular stem cells in the implantation site of plucked hairs??

Right - such a procedure is called IN VIVO HAIR FOLLICLE STEM CELLS MULTIPLICATION.

An implanted HAIR SHAFT, with sufficient attached hair follicle stem cells (see pic above), has basically the same potential to regenerate a completely intact HAIR FOLLICLE again; same as the follicle in the donor site, whose HAIR SHAFT, with the attached stem cells, has been removed due to plucking it out from the follicle.

So everything you have to do is "to mimic the same regeneration environment & conditions" for the IMPLANTED hair shaft in the recipient site. In other words, you simply have to mimic the same situation & conditions as this happens by nature (without "simply adding ANY stem cells") to a hair follicle, whose HAIR SHAFT has been plucked out.

So how can I tell the hair shaft with the attached (but insufficient) stem cells, to multiply its attached stem cells ON ITS OWN to create finally a brand new whole and intact hair follicle? - as this happens also on its own in the donor site - you know, the reason why a plucked hair grows back on its own - and without Dr. Nigams "FDA certificated stem cells Lab"...

Yeah, doing this, telling the HAIR SHAFTS with the attached follicular stem cells to multiply its attached stem cells OWN ITS OWN in the skin - this is called "rocket science" - or sometimes "Illusion" by idiots in this field ...

[drnigams]

IM,read your own reference properly...
Abstract
BACKGROUND AND OBJECTIVES:

A considerable portion of the hair follicle remains attached to plucked hair and can be used for follicle cell culture. In this study we have phenotyped these cells in an attempt to identify the stem cell fraction. Reports in the literature have indicated that this cell population may be positive for cytokeratin (CK) 19. Because stem cells in general need to be protected from apoptosis, the presence of the apoptosis-suppressing Bcl-2 protein, together with the absence of the apoptosis-promoting Bax and the CK profile may be used as an indicator of the stem cell population in the hair follicle, and in cultures of hair follicle cells.
METHODS:

Hair follicles from skin biopsies and plucked hair were derived from the scalps of healthy volunteers. Follicular cells were cultured from the plucked hairs. These hair follicles, plucked hairs and cultured cells were examined for their CK profiles, which are indicative of the type of cell (basal/stem cells) and for their status with respect to the proliferation marker Ki-67, Bax and Bcl-2.
RESULTS:

We found coexpression for CK19 and Bcl-2, but not Bax in two distinct areas, localized in the upper and lower third of the follicle from both skin biopsies and plucked hairs, while proliferation markers were negative in these areas. CK19 and Bcl-2 were also coexpressed in combination in a fraction of the follicular cell culture. The skin basal cell marker CK14 could be found throughout the outer root sheath of the hair follicle from both skin biopsies and plucked hairs, as well as in the follicular cell culture.
CONCLUSIONS:

Thus, CK19/Bcl-2-positive and Bax-negative cells can be obtained from cells derived from plucked hair and are retained in cultures made from these cells. If this phenotype represents follicular stem cells, our finding endorses the assumption that stem cells are located in the bulge area of the hair follicle, as we did not find them in or near the dermal papilla.

Dear im,You no nothing about stemcells.....
different types of stemcells are inherently present in a follicle,even now on your scalp.
Now if you pluck/bisect an angen follicle...and you get the bulge area of stemcells(epithelial) with it..some follicles may have the POTENTIAL to regrow,even without the dermal(mesenchymal) stemcells.As has been shown by few studies including jahodas.
You are missing the most important part of the study you have coated.
REMEMBER THE WORD CULTURE mentioned in the study.
CULTURE CANNOT BE DONE IN ILLUSIONARY PRESERVATION MEDIA...BUT IN THE LAB AS BELOW

Refer to my answers to roger at HS.
Roger,
You have raised i right question,i knew members were not able to understand this difference as in when how can stemcells be converted to activated or progenitor stemcells,which can be of some use.All other promoted concepts like preservation media etc. are an attempt in hoping to activate stemcells,but as on today with not any significant success.
This is how follicle stemcells are converted to active or progenitor cells--
1)a follicle need to be extracted from scalp/body.
2)This follicle need to be removed of blood ,sorrounding tissue,sorrounding cells even of the follicle itself,by specific tissue engineering process which we ,aderans and other biotech companies do.
3)once the stemcells are isolated and there is no contamination of any other cell,including not even any other cell of follicle also.it is ready for further activation and multiplication,this contamination can be checked and ruled out at the lab,including the contamination of organisms,in that case sample is discarded as of no use.
4)After isolation of pure stemcells population of the follicle(stemcells in a follicle like-cd34+,k19 bulge epithelial stemcells,outer root sheath stemcells.dermal cup sheath and mesenchymal dermal stemcells of the root of the follicle,as you are aware all the epithelial and dermal stemcells are required to be able to create a new follicle.)
these stemcells are kept in 4degree centigrade for specific time an an incubator with specific ENZYME which loosens the cell membrane of stemcells( which leads to a series of sodium potassium pump and other biochemical changes in cell meniu),.Active or progenitor cells means now they can divide themselves and multiply and proliferate..
after this the stemcells are kept at warmer temperature in co2 incubator at 37degreecentigrade for a specific time with another specific ENZYME for breaking loose the stemcells IT IS ONLY KNOW THE STEMCELLS COME INTO ACTIVE PROGENITOR CELLS AND WITH ADDITION OF FURTHER GROWTH FACTORS AND RIGHT CULTURE MEDIA THE STEMCELLS ARE HELPED IN SPEEDING UP CELL DIVISION AND THUS MULTIPLICATION.

I hope know you understand that just dipping ,or soaking the follicle in preservation media ,nothing will happen as i said before it is an ILLUSION for marketing and postioning as if using stemcells as the stemcell word is in news,even if you put every possible activator which we use in lab to activate follicle stemcells.
Even if we have to give active stemcells injection like aderans ,we have to use these active stemcells and try to activate the existing dormant follicle stemcells ,without much success for long term,these active stemcell can temporarily stimulate the follicle.
I also plan to extract the vellous or dormanting follicles,activate only the bulge stemcells,implant the rest of the follicle back and reinject the activated stemcells.similarly extract a follicle remove the dermal papilla,activate the dp cells,implant the rest of the follicle back and than inject the activated stemcells of dp back.
I may not need all this ,the day(2months from now) i am able to see the results of stemcell solution and dp culture injection to form new follicle itself,for which i am optimistic.
Yes one may require hm ,dpculture injections once a year or few years to save the fibrosing follicle in non safe donor zone.Follicles from safe donor area will not have this problem as they are safe from genetic and dht effect of fibrosing or dormanting.

Excuse me, but where is the "ultimate proof" of this "ILLUSION" you're TALKING about?
Where is your proof for your incompetent and fraudulent claims?

But let me explain you (again) how stupid and incompetent you are...

EVERYBODY out there - even laymen! - know, that if you pluck, I mean, if your really PLUCK out an anagen hair with out fingers or tweezers or similar "tools" - it grows back...


So what part of this don't you understand?

Furthermore, researchers out there know since DECADES, that follicular stem cells are still attached to PLUCKED hairs...

The scientific evidence for that is simply there since DECADES ...

http://www.ncbi.nlm.nih.gov/pubmed/15149497

Anyway, if there are follicular stem cells attached to the plucked hairs - where do these MISSING follicular stem cells come from, when the hair follicle completely regenerates itself and its hair shaft grows back??

WHERE DO THE MISSING FOLLICULAR STEM CELLS (due to hair plucking) COME FROM??
Definitely not from Dr. Nigams "FDA certificated stem cells Lab"...

So what part of this don't you understand?

Completely the same for the recipient area,
IF you try to IMPLANT plucked hairs:

A portion of the hair follicle stem cells are attached to the plucked hairs, and if this plucked hair regenerates COMPLETELY also in the recipient area - WHERE THE HELL DO THE MISSING FOLLICULAR STEM CELLS COME FROM??

In simple words:
What exactly multiplies the missing follicular stem cells in the implantation site of plucked hairs??

Right - such a procedure is called IN VIVO HAIR FOLLICLE STEM CELLS MULTIPLICATION.

An implanted HAIR SHAFT, with sufficient attached hair follicle stem cells (see pic above), has basically the same potential to regenerate a completely intact HAIR FOLLICLE again; same as the follicle in the donor site, whose HAIR SHAFT, with the attached stem cells, has been removed due to plucking it out from the follicle.

So everything you have to do is "to mimic the same regeneration environment & conditions" for the IMPLANTED hair shaft in the recipient site. In other words, you simply have to mimic the same situation & conditions as this happens by nature (without "simply adding ANY stem cells") to a hair follicle, whose HAIR SHAFT has been plucked out.

So how can I tell the hair shaft with the attached (but insufficient) stem cells, to multiply its attached stem cells ON ITS OWN to create finally a brand new whole and intact hair follicle? - as this happens also on its own in the donor site - you know, the reason why a plucked hair grows back on its own - and without Dr. Nigams "FDA certificated stem cells Lab"...

Yeah, doing this, telling the HAIR SHAFTS with the attached follicular stem cells to multiply its attached stem cells OWN ITS OWN in the skin - this is called "rocket science" - or sometimes "Illusion" by idiots in this field ...

[Arashi]

Ok, you said what you think about Nigam, we ALL GOT IT.

Maybe you got it. But Didi didn't for sure. That's where all the discussion comes from. But now even Didi has, a few pages back in this thread, FINALLY admitted, on page 8 of this thread, that Dr Nigams lies when Dr Nigams does not like the truth, so that part of the discussion can now be left behind.

I just wanted people to see how unreliable this guy is and how many times he lied about stuff. I'd feel really sorry when people go all over to India, just to be treated by some wig wearing clown who has no idea what he's doing.

[drnigams]

IM,

I overestimated your knowledge but can't blame you, you are not a HT surgeon or master in Bio-tech like me.

The picture which you are showing as stem cells in the plucked follicle is a joke, it is the surrounding tissue which is visible like any other FUE / FUT extracted follicle.

The Mid-follicle bulge area where epithelial stem cells are present (which you are mentioning) cannot be seen in human follicle not even the gross bulge without hematoxylin and eosin staining. Yes in mouse follicle a mid-follicular roundish structure is seen under micro scope.

You are trying to tell that the inherent epithelial stem cells present in the safe donor area follicles, can alone create new follicle, you are partially correct not totally. As some may develop into a follicle provided mid-follicular bulge stem cells and outer root sheath stem cells holding follicle part is present in the bisected follicle. Because outer root sheath and dermal cup sheath stem cells have the potential to create missing dermal papilla in the bisected follicle.

Do not forget the important word in the study, Culturing of Stem Cells, not just soaking of stem cells in preservation or cultural media (I can quote Dr. Gho on this on his patent paper as mentioned by him himself, respected Dr. Gho definitely knows about it but not a half doctor like you).

Also remember the safe donor area follicle, stem cells are already active specially after wounding or bisection.

We improve this technique of Jahoda & others by using Ultra Sound and Fibro-optic camera vision and bisect the follicle around Aurber line so that Dermal cup sheath are also present in the bisected follicles (This is the secret, have not shown the picture till now but today I am theoretically revealing for the benefit of all).

To make the bisection regeneration still better and even creating new follicle I inject seperately source DP Cell, Growth Factor, Progenerative Stem Cells into the implantation site of incomplete bisected follicle both at recipient and donot. Automatically better regeneration and new follicle will regenerate.

As on today without any boasting this is the most advance technique of Donor Regeneration. We are still trying to improve with injection of DP Culture and Multiplied Stem cells after 6 weeks.

Kindly follow the post of Roger (Under the Hair loss Research & Clinical trials forum under the thread name "Some questions for Dr. Nigam) on HS wherein he has asked me a very relevant question with my detailed answer about your query I cannot post the link as other forum name is mentioned on the link.

[Arashi]

But unnecessary bashing leads to demotivation.

Now THAT is funny !!! You talking about 'unnecessary bashing', while you not only turn every thread into a Anti Gho, Gho bashing thread, you even do so with the most ridiculous, easily debunkable lies, like that there are 500 extraction sites on James head (Yeah show them to us !), or that picture of NSN scalp you drew where you just COMPLETELY RANDOMLY placed circles.

And you talk about bashing ? If I were Gho I'd send my lawyers after you.

[HARIRI]

Good answer Dr. Nigam, we appreciate your patience in the forum. I hope you wont give up on us. I really have no idea why you are being attacked brutally while you are trying to develop the science of hair restoration!!!

[HARIRI]

I think Mr. Kobren is somehow getting interested in interviewing you especially that you are corresponding pretty well in the forums. He talked a lot about you and Dr. Wesely's scareless FUE in the last episode. He said that he was hoping that you are listening to the show but since you are located in India and because of the massive time difference. I found you the YOU TUBE link of last episode of Bald Truth show.

Please listen to the whole show from this link, its was uploaded yesterday:-

https://www.youtube.com/watch?v=kISP_BdMSrI

Give us your valuable opinion, sir.

[Arashi]

And also, what I just don't get: you keep saying that Gho is a fraud. If so, then how come he got his scientific articles published in the British Journal of Dermatology ? And more importantly: why don't YOU get a scientific article published there in which you explain what Gho did was impossible ? And please copy and paste the email back from the British Joural of Dermatology if they deny publication of your "research" and show us the explanation why they denied publishing your article ok ? That should be fun.

[Arashi]

. I really have no idea why you are being attacked brutally

And this is why I KEEP repeating myself. People come to this board, they haven't read most of the posts and don't know why you are being attacked brutally, they might even feel sorry for you, since they don't know what you did, which lies you told and how you cheated.

But then again, I DO ask myself, what am I even doing here. If people aren't interested in the truth, why should I be the one that is to explain them, time after time ..

[drnigams]

I never said dr gho is this or that and only commented on his technique specially when arashi and im instigate ...my silence will be taken as acceptance ...
Lets not get personal....all of us have right to debate on techniques...thanks arashi..your biased bashing makes me work harder and faster.
.Arashi;116233]And also, what I just don't get: you keep saying that Gho is a fraud. If so, then how come he got his scientific articles published in the British Journal of Dermatology ? And more importantly: why don't YOU get a scientific article published there in which you explain what Gho did was impossible ? And please copy and paste the email back from the British Joural of Dermatology if they deny publication of your "research" and show us the explanation why they denied publishing your article ok ? That should be fun.[/QUOTE]

[Arashi]

I never said dr gho is this or that and only commented

You never used the term 'fraud', I will give you that, but you implied it many many times. You literally said that you were going to 'expose dr gho for what he really is'. Then I ask you: "YES please do that ! Write up a scientific article in which you 'expose' Gho and and send it to the British Journal of Dermatology'. Wouldn't that be the best way to 'expose' Gho ?

[gc83uk]

Why do these gimps come on the forums with about 40odd posts, giving it the 'big I am' attitude without realising that Dr Nigam wants the forum members to join in with his developments and not just lick his ass. He wants people to challenge his theories.

Some people need to check the facts and not worry too much about the wellbeing of others, it's just a forum ffs.

[drnigams]

Thanks HARIRI,
Yesterday i got a mail from a regular senior caller of spencer's show...he has been asking me for some time to call on the show...and don't want to be seen as promoting myself by calling...although as per this guys request i will mail winston or spencer..to give a suitable date and time to cal...i don't want to disturb his topic of the show by calling in between.
I want to communicate a simple technique which all fut/fue docs can use which will give no fue dots and reduce strip scar by 70%,this product is approved for this claim,and readily available and used by surgeons worlwide.
I remember he mentions you as a regular caller from dubai...i will be in doha and dubai..next month..after i return from uk,amsterdum in first week of may.
Few forum guys are meeting me at edinburg,london and amsterdum.
i also plan to take video interview with top stemcell research guys at 7th world congress at edinburgh and post for you all,in these interview i will go into details of hm,dp culture,plucked follicle regen etc.
I

I think Mr. Kobren is somehow getting interested in interviewing you especially that you are corresponding pretty well in the forums. He talked a lot about you and Dr. Wesely's scareless FUE in the last episode. He said that he was hoping that you are listening to the show but since you are located in India and because of the massive time difference. I found you the YOU TUBE link of last episode of Bald Truth show.

Please listen to the whole show from this link, its was uploaded yesterday:-

https://www.youtube.com/watch?v=kISP_BdMSrI

Give us your valuable opinion, sir.

[Arashi]

Anyway, i AM done with you Dr Nigams. Like I've said, if people refuse to read past posts, then why should I be the one to explain them time after time about everything you lied about and your deceit ? If they decide to go to India without doing their due diligence, then thats THEIR problem, not mine.