A case of NW7 for in-vivo Doubling / HM / DP - Day 1 @ Dr. Nigam's

[ccmethinning]

Dr Nigam did not take these pictures, they were taken by the patient - and I honestly dont see any drastic change in lighting.

The first picture is taken in natural light, no flash. The second up close to an incandescent bulb. The third with a flash.

[UK_]

The first picture is taken in natural light, no flash. The second up close to an incandescent bulb. The third with a flash.

Yeah but theoretically the last picture (flash) should have therefore shown the least amount of hair growth (given the degree of light reflecting off the scalp due to the flash) but it actually shows the most.

Regardless, I havent seen any doctor in the past 10 years work as hard as Dr Nigam in helping us cross the bridge to the next best treatment. He's even outlined a complete agenda he has completed (on our behalf) for the congress in a few days time (view post "Visiting WCHR 2013, UK to improve on HM – Agenda @Dr. Nigam’s" on Hairsite).

I dont see any other doctor doing this for us.

[UK_]

I took the liberty of posting the entire agenda:

Visiting WCHR 2013, UK to improve on HM – Agenda @Dr. Nigam’s

My agenda for 7th World Congress on Hair Research, 4th – 6th May 2013 would be seeking answer for following questions by meeting concerned researchers and discussions with them during their presentation. Let me see if I can get video interviews, discussions for you all. All my member friends can also post your questions related to stem cell hair multiplication, DP Cells Injection, DP culture, etc which I might have missed.

I will be in Edinburgh from 3rd – 6tth May & 10th & 11th May 2013, I will in London from 7th – 9th May 2013. Let me know if any of the forum members from UK or around who will be there. Kindly let me know on dr.rahul1970@gmail.com
One member from Netherland will be coming to attend the conference, he is a bio-tech and has been helping me a lot may be I will sponsor him partly.

Questions:-
1. Hair follicle epithelial and mesonchomil stem cells interaction and communication to create a new hair follicle in-vitro or in-vivo. Would like to discuss more with Jahoda regarding his organ germ method culture for the same.

2. Trichogenic DP culture, Cohesive DP Structure, In capsulated DP Cells, ECM secreting aggregates of DP cells.

3. How to increase the healing of bisected follicle without the Dermal Papilla?

4. How potent are freshly isolated DP cells from body hair kept in DP culture medium?

5. The in-vitro follicle bisection, what is the minimum requirement for re growth in the bisected follicle (Bulge stem cells + outer root sheath & / or dermal cup sheaths stem cells &/or hair germ matrix epithelial stem cells)?

6. The best method in culture medium for DP culture that is 3D culture hang-in-drop method, how to get a intact basal lamina?

7. Role of repeat injection of Growth Factors in-vivo for activating dormant follicles &/or creating new follicles.

8. Better ways to counter the effects of DHT on Dermal Papilla via TGF-BETA1, how to block or slow down the follicle miniaturization?

9. What difference in hair follicle neogenesis we can expect viv-a-vis multipotent adult hair follicle stem cells vs pluripotent embryonic stem cells? How effective wil be the conversion of multipotent adult hair follicle stem cells to pluripotent embryonic stem cells? How will HM improve if at all by conversion of multipotent adult hair follicle stem cells to pluripotent stem cells and subsequent differentiation?

10. What is my favorite researcher Jahoda’s experience on trans-gender hair transplant and how to achieve optimal results with eye-brow like pluck hair transplant with or without associated Dermal Sheath?

11. How many of these researchers are open to do small clinical experiment on their research findings at Mumbai, India in association with my Clinic & Lab to take advantage of my existing permission regarding stem cell research and software regulations in India?


I want to focus on following research papers and subsequent Q&A discussions from 4th – 6th May 2013, Edinburgh, UK:-

1. Update on dermal papilla culture systems
Young Wang Sung, Republic of Korea
2. Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches
Koh-ei Toyoshima, Japan
3. Classic perspectives on human hair follicle organ culture
Michael Philpott, UK
4. Intermediate hair follicles: a more clinically relevant hair follicle model
Ben Miranda, UK
5. Tissue Engineering and Regeneration
Chairs: Mike Philpott, Bruno Bernard, Kurt Stenn

P217 - Full-thickness skin with mature and cycling hair follicles using tissue culture expanded human cells
X Wu, L Scott, K Stenn
Aderans Research Institute, Inc., Marietta, United States

P218 Enhancing hair follicle neogenesis in bioengineered human skin substitutes
R Thangapazham1, P Klover1, S Li1, J-A Wang1, Y Zheng2, L Sperling1, G Cotsarelis2, T Darling1
Uniformed Services University of the Health Sciences, Bethesda, United States,
2University of Pennsylvania, Philadelphia, United States

P220-Human hair follicle neogenesis using microenvironmentally reprogrammed dermal papilla cells
CA Higgins1, CA Jahoda2, AM Christiano1,3
1Dermatology, Columbia University, New York, United States, 2 Biological and Biomedical Sciences, Durham University, Durham, United Kingdom, 3Genetics and Development, Columbia University, New York, United States

P221 Calcium microcapsule: a possible scaffold for building artificial dermal papillae
C-M Lin1, K Huang2, Y Li3
1Shantou University Medical College, Shantou, China, 2S. Gallicano Dermatologic
Institute (IRCCS) Second Affiliated Hospital, Shantou University Medical College,
Shantou, China, 3First Affiliated Hospital, Shantou University Medical College, Shantou,
China

P222 Human scalp derived fibroblasts are multipotent and trichogenic
X Wu, K Watkins, K Stenn
Aderans Research Institute, Marietta, United States

P044 The therapeutic potential of naturally immune privileged hair follicle cells
X Wang, J Hao, G Leung, T Breitkopf, Z Ao, G Warnock, J Shapiro, K McElwee
University of British Columbia, Vancouver, Canada

8:30-Concurrent Session: 3 Sidlaw Auditorium
Stem Cells and Epigenetics
Chairs: George Cotsarelis, Vladimir Botchkarev, Denis Headon

P186 Topology-dependent plucking leads to more regenerating hairs than plucked via extra-follicular macro-environment modulation
C-C Chen1,2,3, L Wang4, TX Jiang1, S Shi4, CM Chuong1
1Pathology, University of Southern California, Los Angeles, United States,
2Department of Dermatology, Taipei Veterans General Hospital, Taipei, Taiwan,
Republic of China, 3Department of Dermatology, Yang ming University, Taipei, Taiwan,
Republic of China, 4Center for Craniofacial Molecular Biology, University of Southern
California, Los Angeles, United States

P187 Competitive balance of Intra-bulge BMP/WNT signaling reveals a robust gene network governing stem cell homeostasis and cyclic activation
E Kandyba1, Y Leung1, Y-B Chen2, R Widelitz3, C-M Chuong3, K Kobielak1
1Eli and Edythe Broad Center for Regenerative Medicine & Stem Cell Research,
Department of Pathology, University of Southern California, Los Angeles, United States,
2Norris Medical Library, University of Southern California, Los Angeles, United States,
3Department of Pathology, University of Southern California, Los Angeles, United States
1030 Plenary Symposium 1: Pentland Auditorium
An Update on Hair Follicle Stem Cells
Chairs: Satoshi Itami, Rod Sinclair

PS1.1 Hair follicle stem cells in alopecia
George Cotsarelis, USA

PS1.4 Cellular and signaling mechanisms that regulate hair follicle stem cells by live imaging
Valentina Greco, USA

PS1.2 Mechanisms of hair follicle aging and stem cell regulation
Emi Nishimura, Japan

PS1.3 Biological and translational potential of hair follicle mesenchymal cells
Colin Jahoda, UK

1200 Keynote Lecture Pentland Auditorium
K1 Skin Stem Cells: In Silence and in Action
Elaine Fuchs, USA

1600 Concurrent Session 5: Pentland Auditorium
Androgenetic Alopecia and Hair Restoration
Chairs: Ramon Grimalt, Nilofer Farjo, Venkataram Mysore

P039 What is the meaning of the “visible pigmented non-vellus hair count” in FDAapproved clinical trial reports?
DJJ Van Neste1,2, HD Rushton3
1Skinterface, Tournai, Belgium, 2Brussels’ Hair Clinic, Brussels, Belgium, 3School of
Pharmacy & Biomedical Sciences, University of Portsmouth, Portsmouth, United
Kingdom

P040 Pharmacodynamic of P-3074 (finasteride 0.25% topical solution) in subjects with androgenetic alopecia
M Caserini1, R Palmieri1, M Radicioni2, E Terragni2
1Polichem S.A., Lugano, Switzerland, 2Cross Research S.A., Arzo, Switzerland


P041 Genetic variations associated with efficacy of dutasteride, the dual 5a-reductase inhibitor in the treatment of male pattern hair loss
SJ Jo1,2, A Rhie3,4, S Lee3,4, B-L Lew5, W-Y Sim5, KH Cho1,2, JH Chung1,2, J-I Kim3,4, OS Kwon1,2
1Department of Dermatology, Seoul National University College of Medicine, Seoul,
Korea, Republic of, 2Institute of Human-Environment Interface Biology, Seoul National
University College of Medicine, Seoul, Korea, Republic of, 3Genomic Medicine Institute,
Medical Research Center, Seoul National University, Seoul, Korea, Republic of,
4Department of Biomedical Sciences, Seoul National University Graduate School, Seoul,
Korea, Republic of, 5Department of Dermatology, Kyunghee University College of
Medicine, Seoul, Korea, Republic of

P043 Oxidative stress and cell senescence in androgenetic alopecia (AGA)
JH Upton1, A Bahta1, N Farjo2, B Farjo2, R Bhogal3, MP Philpott1
1Centre for Cutaneous Research, Queen Mary’s University of London, Whitechapel,
United Kingdom, 2Farjo Medical Centre, Manchester, United Kingdom, 3Unilever R&D,
Colworth, United Kingdom

P227 New development in unlimited hair follicle transplant
C-M Lin1, K Huang2, Y Li3
1Shantou University Medical College, Shantou, China, 2Second Affiliated Hospital,
Shantou University Medical College, Shantou, China, 3First Affiliated Hospital,
Shantou University Medical College, Shantou, China

P046 Platelet-rich plasma promoting hair growth in human hair follicle dermal papilla cells associated VEGF and VEGFR-2
BJ Kim1,2, SY Kim1,2, TR Kwon1,2, YH Lee1, YS Song1,2, K Li1, YW Lee3, CH Huh4, CK
Hong1
1Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea,
Republic of, 2Department of Convergence Medicine and Pharmaceutical Biosciences,
Graduate School, Chung-Ang University, Seoul, Korea, Republic of, 3Department of
Dermatology, Konkuk University, College of Medicine, Seoul, Korea, Republic of,
4Department of Dermatology, Seoul National University Bundang hospital, Seongnam,
Korea, Republic of

1600 Concurrent Session 6: Fintry Auditorium
Factors Influencing Hair Growth
Chairs: Ryoji Tsuboi, Julie Thornton, Natalia Botchkareva

P126 A novel PPARγ modulator (GMG-43AC) inhibits human hair growth and inflammatory response in keratinocytes, and up-regulates expression of epithelial stem-cell associated keratins, K15 and K19
Y Ramot1,2, A Mastrofrancesco3, E Herczeg-Lisztes4, T Biro4, M Picardo3, JE Kloepper2,
R Paus2,5
1Department of Dermatology, Hadassah - Hebrew University Medical Center, Jerusalem,
Israel, 2Department of Dermatology, University of Luebeck, Luebeck, Germany,
3Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics
Research, S. Gallicano Dermatologic Institute (IRCCS), Rome, Italy, 4Department of
Physiology, University of Debrecen, Debrecen, Hungary, 5Institute of Inflammation and
Repair, University of Manchester, Manchester, United Kingdom

P127 Influence of oxygen tension on hair growth and cycle and therapeutic potential of systemic normobaric oxygenation
H Kato1, K Kinosheeta1, N Aoi1, K Inoue1, S Itami2, K Yoshimura1
1Department of Plastic Surgery, University of Tokyo Hospital, Tokyo, Japan,
2Department of Regenerative Dermatology Osaka University Graduate School of
Medicine, Osaka, Japan

P128 Eplerenone stimulates human hair growth ex vivo
JE Kloepper1, S Klominek1, T Biró2, N Farman3, R Paus1,4

P129 Age related expression changes of WNT signaling associated genes in human hair follicle
SN Kim1, AR Kim1, SY Lee2, WS Park1

P130 Human scalp hair follicles possess the enzymes to synthesise prostaglandins and prostamides from phospholipids and contain PGFα in vivo
KG Khidhir1,2, M Shalbuf1, NP Farjo3, BK Farjo3, JW Wang4, DF Woodward4, SM
Picksley1, VA Randall1

P131 Closing KATPATP channels in human hair follicles inhibits hair growth, promotes catagen-like changes and alters paracrine signalling; could this be a new therapeutic approach for excessive hair disorders?
H Rahman1, N Farjo2, B Farjo2, V Randall1

P132 Effect of hair follicle environment on nanocarriers stability and drug release properties
F Rancan1, S Hadam1, Z Afraz1, B Bellier2, R Wagner3, C Graf4, J Lademann5, E Ruehl4,
B Verrier6, U Blume-Peytavi1, A Vogt1


P133 Thyrotropin-releasing hormone (TRH) modulates keratin expression in human skin
Y Ramot1,2, G Zhang2, J Hardman3, IS Haslam3, T Biro4, L Langbein5, R Paus2,3

P134 Systemic angiotensinergic stimulation modulates Epithelial-Mesenchymal Transition and collagen deposition in skin and hair follicle
ZP Jara1, M Consolo2, SP Panicker2, K Honda2, P Karnik2, S Karnik1
1Cleveland Clinic, Cleveland, United States, 2Dermatology, CWRU, Cleveland, United States

1600 Concurrent Session 7: Sidlaw Auditorium
P145 Trichomegaly, or Hollywood “movie lashes” resulting from mutations in FGF5 and an elongated hair cycle anagen phase
CA Higgins1, L Petukhova1,2, YY Ho1, E Drill1, L Shapiro3, M Wajid4, AM Christiano1,5

P146 Detection of chronobiological features on the surface of human hair through the multi-stage analysis of Wilhelmy-force profiles

P147 Fractions of cortical cell types correlate with macroscopic bending modulus inhuman hair
T Itou1, Y Ezawa1, S Nagase1, A Mamada1, S Inoue2, K Koike1
1Kao Corporation, Tokyo, Japan, 2Kao Corporation, Wakayama, Japan

P148 In vitro assembly of human hair keratin intermediate filaments: Combination of Keratin proteins and their properties

P149 ALOX12 inhibitors promote cuticle maturation that affects on hair Texture
K Kizawa1, T Takahashi2, T Fujimori3, R Suzuki2, T Kawai3

1600 Psychology and Hair Disorders Workshop Tinto

1605 Psychodermatology and psychopharmacology of hair loss
John Koo, USA
Monica Huynh, USA

1800 EHRS General Assembly Kilsyth / Moorfoot
1800 KHRS General Assembly Tinto

Monday 6 May 2013
0830 Concurrent Session 10: Sidlaw Auditorium Hair Follicle Development, Control of the Hair Cycle and Pigmentation
Chairs: Cheng-Ming Chuong, Emi Nishimura, Desmond Tobin

P103 Functional presence of the endocannabinoid system in human hair follicle derived outer root sheath keratinocytes
E Herczeg-Lisztes1, IL Szabó1, A Oláh1, AG 1, N Vasas1, K Sugawara2, R Paus2, T Bíró1

P151 Regulatory T cells increased in the area around and above the bulge of mouse vibrissa follicles in catagen
M Oishi, I Niibe, T Matsuzaki
Biological Science, Shimane University, Matsue, Japan

P152 A meeting of two chronobiological systems: Period1 and BMAL1 modulate the human hair cycle
Y Al-Nuaimi1, JA Hardman2, IS Haslam2, T Biro3, BI Toth3, N Farjo4, B Farjo4, M Philpott5, R Watson2, B Grimaldi6, JE Kloepper7, R Paus2,7

P153 Reprogramming regular skin fibroblasts into hair inducing dermal papilla cells
C Clavel1,2, A Rezza1,2, M Rendl1,2,3

P154 Development of the mouse dermal fat layer is linked to hair follicle development, occurs independently of subcutaneous fat and is marked by restricted early expression of FABP4
K Wojciechowicz1,2, K Gledhill1, CA Ambler1, C Manning1, CA Jahoda1

P155 ATM expression in hair bulb melanocytes is a marker of oxidative stress in canities-prone scalp
SK Sikkink1, O Freis2, L Danoux2, DJ Tobin1
P156 Stem cell factor/c-kit signalling in the hair bulb is essential to maintain hair pigmentation in adult human hair follicles in vivo and in organ culture
T Vafaee1,2, TJ Jenner1, SM Picksley1, VA Randall1

1200 John Ebling Lecture Pentland Auditorium

K2 Hair Follicle: The Root of the Future. Clinical Research, Implications and Advances
Ulrike Blume-Peytavi, Germany

1300 Hair Research Society of India Symposium Fintry Auditorium



CP1 What’s new in clinical hair research?
Abraham Zlotogorski, Israel

CP2 What’s new in basic science aspects of hair research?
Sarah Millar, USA

[One]

What is this?

[Vox]

What is this?

Perhaps our wet dream? Whatever it may be, it broke my bullshitometer. Unless proved otherwise of course.

[drnigams]

One,
Diagramatic representation of stemcell hair multiplication procedure...In my print advt.s this explanation is used for layman understanding...
The success rate of hm will increase with 3d spheroidal dp culture in coming months..At present some get great results and some get mediocre results and some no results.
Hope fully with trichogenic dp culture in coming months ..no more doubling will be required...

What is this?

[bananana]

Dr Nigam, a wild idea here:

Is it in any way possible to determine whether the patient will be a good candidate for HM before his visit? Maybe by patient sending some kind of specimen via mail to your clinic or something similar?

Because 20% of patients will get no results, and that is a pretty big number, and also are you planning to develop the technique in the future (to reduce that number and/or get better results)?

Thanks

[drnigams]

bananana,
Aderans are working on a concept, wherein with genetic testing they can predict ,which patients will respond to autologous stemcell solution.

Yes ,in coming months with injections of 3d spheroidal dp culture,cohesive ecm secreting dermal papilla ,encapsulated dermal papilla,proto hair development in lab invitro and implantation should improve success rate of HM injections.
We will give 1st dp culture injection to our patient in 4 weeks from now and will observe the results in coming months,followed by above mentioned improvements.
Even Aderans who have much longer experience than us are able to provide similar results with their HM injections as on today in their clinical trials.
HM injections in combination of trichogenic dp culture injections with or without growth factors should give much improved results in months to come...

Dr Nigam, a wild idea here:

Is it in any way possible to determine whether the patient will be a good candidate for HM before his visit? Maybe by patient sending some kind of specimen via mail to your clinic or something similar?

Because 20% of patients will get no results, and that is a pretty big number, and also are you planning to develop the technique in the future (to reduce that number and/or get better results)?

Thanks

[UK_]

Dr Nigam regarding the 20% non-responders, are these only after 1 treatment? Have you tried 3 - 4 treatments at separate time-points and still no response?

I think ill be getting a treatment when the trichogenic dp culture injections are released.

[bananana]

bananana,
Aderans are working on a concept, wherein with genetic testing they can predict ,which patients will respond to autologous stemcell solution.

Yes ,in coming months with injections of 3d spheroidal dp culture,cohesive ecm secreting dermal papilla ,encapsulated dermal papilla,proto hair development in lab invitro and implantation should improve success rate of HM injections.
We will give 1st dp culture injection to our patient in 4 weeks from now and will observe the results in coming months,followed by above mentioned improvements.
Even Aderans who have much longer experience than us are able to provide similar results with their HM injections as on today in their clinical trials.
HM injections in combination of trichogenic dp culture injections with or without growth factors should give much improved results in months to come...

Thanks you for a prompt response, as always. I cant wait to see the results from your improved technique. I hope the successful HM percentage will go up with the introduction of these new techniques.

Keep us updated, and if you can get in touch with OBI ask him how is his progress going.

EDIT: UK's question is interesting - have you tried multiple HM sessions on non responders?

Thanks

[stayhopeful]

seriously, what don't you understand about controlled lighting? let's be adults here

[mm05035]

One,
Diagramatic representation of stemcell hair multiplication procedure...In my print advt.s this explanation is used for layman understanding...
The success rate of hm will increase with 3d spheroidal dp culture in coming months..At present some get great results and some get mediocre results and some no results.
Hope fully with trichogenic dp culture in coming months ..no more doubling will be required...

Dr Nigam,
I would kindly like to ask you one thing. I know that the hairloss industry is very profitable and selling hope to hairloss sufferers could result to a fortune for you, but please keep in mind that among the hairloss sufferers there are young people like me who are really depressed and hairloss affects our everyday lives. By making bold claims like 300-->60000 hairs you make me postpone other alternatives i have right now and wait for your treatment.
I am not asking for your sympathy here I am just asking you to be honest. You are actually playing with our lives here. Please don't promise things you won't be able to achieve.
Many in here believe in you, even though this is unreasonable, so please don't let us down.

[TO YOUNG TO RETIRE]

300 -> 60000

[TO YOUNG TO RETIRE]

that ,i mean what are we doing here waiting and arguing and being emotionaly exhausted, ????????????

why the f@## we dont visit this Dr?

[Vox]

300 -> 60000

Do you really believe these numbers can hold any truth?