50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

**Joker** · 04-18-2013, 11:02 AM

The macro photos of this 15-graft in-vitro test were posted on HS by Dr. Nigam, showing total regrowth in 18 days. Macro photos of NSN's 10-graft in-vivo test showed 90% regrowth.

And Spencer still won't even consider talking to Dr. Nigam? I'd say this is enough proof to establish that the guy is doing at least something out of the ordinary. It fits right within Dr. Wesley's and Dr. Gho's research (both of whom Spencer has taken an interest in) and might even help both.

**TO YOUNG TO RETIRE** · 04-18-2013, 11:06 AM

cannot agree more

Originally posted by Joker

The macro photos of this 15-graft in-vitro test were posted on HS by Dr. Nigam, showing total regrowth in 18 days. Macro photos of NSN's 10-graft in-vivo test showed 90% regrowth.

And Spencer still won't even consider talking to Dr. Nigam? I'd say this is enough proof to establish that the guy is doing at least something out of the ordinary. It fits right within Dr. Wesley's and Dr. Gho's research (both of whom Spencer has taken an interest in) and might even help both.

**UK_** · 04-18-2013, 11:13 AM

Originally posted by Joker

The macro photos of this 15-graft in-vitro test were posted on HS by Dr. Nigam, showing total regrowth in 18 days. Macro photos of NSN's 10-graft in-vivo test showed 90% regrowth.

And Spencer still won't even consider talking to Dr. Nigam? I'd say this is enough proof to establish that the guy is doing at least something out of the ordinary. It fits right within Dr. Wesley's and Dr. Gho's research (both of whom Spencer has taken an interest in) and might even help both.

Totally agree, there is clear regrowth here - I find it almost absurd that Spencer has not even acknowledged this yet.

Perhaps we should find another forum?

**534623** · 04-18-2013, 11:28 AM

Originally posted by Joker

The macro photos of this 15-graft in-vitro test were posted on HS by Dr. Nigam, showing total regrowth in 18 days. Macro photos of NSN's 10-graft in-vivo test showed 90% regrowth.

And Spencer still won't even consider talking to Dr. Nigam? I'd say this is ...

Sure, YOU would say - more experienced guys surely not; or could you see what happened with the extracted part(s)??

Because to create (for photos) "small extractions wounds" and to show idiots "100% regrowth"!!" thereafter ...

Haarweb Forum - Los bericht bekijken - hair science institute methode werkt?

http://www.haarweb.nl/forum/showpost.php?p=260703&postcount=10

... every idiotic HT doc out there can show that.

By the way: Didn't you notice that he just shows "donor regrowth photos of extremely small areas? And who says that these pics belong to "the" discussed patient?

Ahh, I know - you think Nigam is just unable to make photos of the WHOLE (or at least big parts) of a patients donor area?

Here is the the proof that he IS able to take such photos ...

Dr. Nigam's fraudulent claims about Dr. Gho's work - Bald Truth Talk - Hair Loss, Hair Transplant and Hair Restoration Community

http://www.baldtruthtalk.com/showthread.php?t=11858

And here is one of Dr. Nigam's extremely fraudulent claims about Dr. Gho's work... And here you can see in detail what Dr. Nigam, in fact, did in this photo: Dr. Nigam simply made lots of blue circles everywhere in an area behind neversaynever's ear - an area, which simply doesn't (naturally) have a high follicle density

... and everybody could see these photos which show almost the whole donor area.

**Joker** · 04-18-2013, 11:59 AM

Give it a rest, guy. Not everyone on earth is your sworn enemy, and not everyone on earth responds well to the sarcasm and aggression you bring to every conversation. I'm not proclaiming Dr. Nigam as the messiah. I'm saying that every physician - including Gho - deserves an opportunity to present their advances without being heckled. I understand you don't like Nigam, and that's your prerogative, but you also impede his ability to communicate his methods with other users who might not share your opinions. That's not fair.

**Henkeh91** · 04-18-2013, 12:16 PM

Thanks Dr Nigams for sharing

**Arashi** · 04-18-2013, 12:17 PM

Originally posted by Joker

Give it a rest, guy. Not everyone on earth is your sworn enemy, and not everyone on earth responds well to the sarcasm and aggression you bring to every conversation. I'm not proclaiming Dr. Nigam as the messiah. I'm saying that every physician - including Gho - deserves an opportunity to present their advances without being heckled. I understand you don't like Nigam, and that's your prerogative, but you also impede his ability to communicate his methods with other users who might not share your opinions. That's not fair.

Maybe you believe in Dr Nigams, maybe you don't. But IronMan has of course a point here, you can't take NSN's birthmark area seriously as proof. A picture of the WHOLE donor area, now that would be interesting. But NeverSayNever magically disappeared from all forums all of a sudden ... Now a critic might think that he made a deal with Dr Nigam. Maybe that if regrowth failed dr Nigam would pay all made expenses, like plane, hotel etc, if NSN just would never return to the forums again. I don't know. It's just beyond me why an active forum member here wants to travel all the way to India, just to completely disappear shortly thereafter. But hey, thats just me.

Main point is: you can't blame Spencer for not taking Nigams seriously YET. This might change in the future, I might be wrong about Nigams, let's all hope so, but I'm afraid I'm spot on ...

**paradigmshift** · 04-18-2013, 11:52 PM

Arashi, Your NSN theory sounds more like a unlikely conspiracy than anything.

For any individual member, activity on this forum happens in bursts for a few weeks and ceases for some time (this is likely correlated with how depressed and self-conscious the person is feeling at the point in his life). May be NSN is really ecstatic about his results right now... and that's why he is not online every day like you and me. He might be enjoying life.

IronMan is a very special exception. He often raises good points, but they completely drowned out by his hateful, sarcastic, and immature (surprising for his age) rants. If he raises his objections a little more dispassionately, people might not be so annoyed by him. If he becomes incrementally more caring, people might even like him (like they do Desmond). We can't know what he has been through in life... after all, he is on this forum pretty much all the time.

**Arashi** · 04-19-2013, 06:08 AM

Originally posted by paradigmshift

Arashi, Your NSN theory sounds more like a unlikely conspiracy than anything.

For any individual member, activity on this forum happens in bursts for a few weeks and ceases for some time (this is likely correlated with how depressed and self-conscious the person is feeling at the point in his life). May be NSN is really ecstatic about his results right now... and that's why he is not online every day like you and me. He might be enjoying life.

IronMan is a very special exception. He often raises good points, but they completely drowned out by his hateful, sarcastic, and immature (surprising for his age) rants. If he raises his objections a little more dispassionately, people might not be so annoyed by him. If he becomes incrementally more caring, people might even like him (like they do Desmond). We can't know what he has been through in life... after all, he is on this forum pretty much all the time.

Of course you might be right. But the thing that bothers me, that this wasn't a 'normal' case, he was performing a TEST for Dr Nigams. In a normal situation, yes, it would make sense that a person is just happy with his result and leaves the forums. But in a test ? It just doesn't make any sense to me ...

And regarding Ironman, I agree. He's a valuable source to these forums, with often very good info, but his sarcasm just makes you hate him at times ...

**drnigams** · 05-24-2013, 08:53 AM

Day 53 Update In-Vitro Doubling of 15 G patch test @Dr. Nigam's

Day 53 Update In-Vitro Doubling of 15 G patch test @Dr. Nigam's

Dear friends,

Kindly find Day 53 update of donor and recipient of 15 graft patch test of in-vitro hair doubling along with tattoos marks and one shot video.

results
Donor - On Day 0 total 23 follicles were extracted from 15 grafts (Single - 8, Double - 6 & Triple - 1)

On Day 53 by Videoscopic & Macro images of Donor shows - 37 follicles as against 52 follicles on Day 18 may be some must have gone into shedding or some may have not regenerated. Although still better than 23 follicles which were extracted from the donor which mean the gain of 14 follicles at the donor which means as on today (Day 53) regeneration is 160%, may become less or more, actual donor regeneration will come to know in 6 to 9 months. You must be wondering how come more than 100% regeneration that is becuase of stem cells, dp cell, growth factor were injected into the donor and recipient. You can clearly see even after using large punch to extract the graft from the donor like in FUE...... THERE IS NO WHITE DOT OR SCAR MARK. This proves if FUE doctors start using ECM, concentrated PRP and / or growth factor, stem cells, DP cells, visible FUE scar can be avoided. As the above have important role in wound healing. Hence no white dot or scare.

Recipient shows 23 follicles as against 38 follicles on Day 18 but still 100% recipient regeneration as 23 bisected follicle were implanted at the recipient, may be the 15 follicles is not seen on Day 53. May be they must have gone into shedding or they must have not regenerated. Actual recipient regeneration will be known after 6 to 8 month.

observe the pigmentation and thickness of Donor and Recipient re-growth to analyze the diameter of new hair.

WCHR 2013, Edinburgh, UK, had 2 paper presentation on unlimited hair transplant. One Japanese paper were shown which was earlier confirmed by Jahoda that ........
Freshly isolated DP cells which is trichogenic, if implanted at the base of upper half of the amputated / bisected follicles, new dermal papilla or new follicle with similar characteristic of the donor will be created.
Another study confirm that upper half of the hair follicle if amputed and extracted with outer root sheath stem cells minimum and great if extracted with matrix stem cells can create it's own dermal papilla.

The study also confirmed that the lower dermal papilla when imputed from its upper half of the follicle can create it's own shaft and rest of the follicle.

Cotsarelis had published one paper called "De-Differentiation of stem cells" in which bulge stem cells (epidermal) can become matrix germ stem cells and dermal stem cells and similarly dermal stem cells can become epidermal stem cells if required, this is called stem cell De-Differentiation.

With my experience of 4 to 5 month of in-vitro and in-vivo technique, as on today I come to conclusion that in-vitro hair doubling or donor doubling will give higher donor & recipient regeneration with better quality of hair than in-vivo donor doubling.

since in in-vivo donor doubling the major issues is that its a blind technique and not one can bisect the follicle at the matrix germ cells level or dermal cup sheath level. In-vivo technique has disadvantages....
1. since it's a blind technique, precise bisection which we want cannot happen
2. Becuase precise bisection cannot happen some extraction cannot suitable hence multiple extraction need to be done
3. Because it is blind technique hence it will be more expensive
4. The hair re-growth at the recipient will be much lesser than the in-vitro technique because only part of upper follicle is being extracted hence hair at the recipient could be thinner.

Both In-vitro and in-vivo technique will be scarless but the role of injecting stem cells, dp cells, growth factor, ECM, as per my observation is very important for donor and recipient re-growth and thickness of hair.

I remember what Washneik said that in the 1st phase hair multiplication will be used as a adjunct to the hair transplant. This is what I feel too, by the time in coming months we will get consistent result when we will add 3d spheroidal aggregated capsulated, growth factor secretarine trichogenic, good dose of dp cells and / or what Dr. Gerd and Tsuji Lab did that is co-culturing of epithelial stem cells and trichogenic dp cells to create proto hair or micro follicle as named by Gerd for real CELLULAR HAIR IMPLANT or if Histogen succeeds and as believed by Ralf Paus of UK as he told to me in WCHR 2013 that dormant 100000 hair follicles lying also in the slick bald scalp in Telogen phase with partial fibrosis can be activated with stem cells, DP cells, Growth factors, Noggin, WNT7A, etc. in-vivo.

I would advice all the patient of AGA doing or not doing hair transplant, doubling or HM should start with mast cells supressor (Oral or topical) OC-03-01, anti-androgen preferably topical will come in the market, antioxydent like melatonin. Oral good dose of antioxidents and smoker should start L-Cystine and biotin in good doses orally and / or topically to counter the follicle damage of nicotine.

By next month I will have topical vascular endothelial growth factor FGF and others which can be applied topically for the medical AGA prescription but these topical gels are not available to the patient directly but only to the bio-tech labs.
For prevention of AGA I am working on how to reduce on micro inflammation due to multiplication at and around hair follicle and androgenic TGF - Beta-catenine topical or injectible to reduce mast cell released histamines. Reducing DHT concentration on scalp, countering prostaglandin, interlukine 1 & 6 concentration on scalp and products to up regulate PGE2, insulin growth factor, products to prevent apoptosis of stem cells, products to convert dormant stem cells to progenitor stem cells in-vivo through ioantophoresis, electoporation, nano particulate transdermal targetted delivery, chitosan, etc capsulated vehicle.

These medical prescription will also helps my HM results if patient starts this medical prescription, same for the regular transplant.

If I had movable photon microscope costing US $1 million, my HM result would have increase significantly. Since with movable photon microscope applied on patient scalp. I can see telogen follicles on the human scalp and inject the stem cells directly into it specially when they are fibrosed, partially or totally, normal stem cells injection could be difficult to them. In movable photo microscope, I can see cells of epidermis and dermis and also upward & downward movement of stem cells.

As you remember I had mentioned the use of photon micro-scope in cellular regeneration micro imaging study, same was highlighted by 2 presenters with their paper titled "Imaging in hair generative therapies"

Elian Fuchs in her paper presentation "Stem cells in silence and in action" showed upward & downward descent video of moving stem cells in a hair follicle in-vivo

Hope fully I have to do some research in Gerd lab in Berlin which is having fixed photon microscope and bio-reactors, etc to find the answer that why HM doesn't response on all the scalp? and we know HM respond on some scalp and in these responding scalp the results are really good and acceptable.

Donor Count line

Click the below link for enlarge image

http://www.drnigams.net/images/RTN/Day53/Large/Donor/Donor-countline.jpg

Recipient IMG

Click the below link for enlarge image

http://www.drnigams.net/images/RTN/Day53/Large/Recipient/Recipient.jpg