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I know very little about Follica, but it sounds like their technique for regrowing hair is/was a lot more mundane than anything involving cutting-edge developments with growth factors, dermal papilla, stem cells, etc...
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Does the wounding leave scars?Comment
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If anybody is interested in learning about Follica and has a lot of free time:
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Dr Nigams just stated on HS that 2 forum members arrived who will be getting 3d DP culture !! That should be very interesting. If he can repeat Jahoda's experiment on SCID mice, but this time on human beings, man that's going to be extremely interesting !!Comment
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From Jahoda's paper:
Hair Follicle (HF) neogenesis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using cultured DP cells. In contrast , HF neogenesis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact DP , which are capable of neogenesis , but absent in cultured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.
And now Dr Nigams is about to try this on humans !! Really, if this doesn't get you interested, then what does ?
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It seems exciting, i just want to see photos to back it up that is all.From Jahoda's paper:
Hair Follicle (HF) neogenesis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using cultured DP cells. In contrast , HF neogenesis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact DP , which are capable of neogenesis , but absent in cultured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.
And now Dr Nigams is about to try this on humans !! Really, if this doesn't get you interested, then what does ?Comment
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Photographs of results get me interested. That's about it at this point.Comment
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