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Thread: PRP At home

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  1. #11
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    Quote Originally Posted by woodpecker View Post
    Ok, just thought I'll put in my 2 cents. Links are just for references, I'm sure you can find something cheaper online or locally.

    Drawing blood - I'm a phlebotomist. So this I know A LOT. get a butterfly needle, 21g or 23g. Either draw yourself or get someone to help you.
    http://www.plcofamerica.com/blog/201...erfly-needles/

    Needle holders
    - http://www.plcofamerica.com/blog/201...erfly-needles/

    Tourniquets to help your veins pop and easier to draw: https://www.amazon.com/Physician-Sup...ds=Tourniquets

    Tube rack for when you need to keep your tubes upright to pipette the RPR. Also use it to store your unused PRP in freezer (read below): https://www.amazon.com/SEOH-TEST-TUB...ds=tube+holder

    Pipettes to remove plasma from your tubes: https://www.amazon.com/Qingsun-Dispo...words=pipettes

    Look thru YouTube on how to do a simple blood draw: https://www.youtube.com/watch?v=_w3-gtzvenk

    Type of Tubes: Tubes with ACD solutions or simple EDTA works fine too. The trick is to not let the blood clot. Without ACD or EDTA you will only get serum in return and not plasma. That said, I still prefer ACD.

    ACD: https://www.amazon.com/Solution-Tube...ords=acd+tubes

    Sterile tubes
    - you need some sterile tubes to keep your PRP before you transfer to your syringes. Also you can use this to storage your unused PRP and keep them in the freezer. Plasma is usable for up to one year if store in freezer. So keep in mind to draw a little more blood each time. When ready to use the frozen PRP, just thaw them in room temperature. https://www.amazon.com/EarthOx-Steri...=sterile+tubes

    Centrifuge: https://www.amazon.com/Tangkula-Elec...rds=centrifuge


    Ok here are the steps:


    1. Draw your blood in ACD tubes. maybe 6-8 tubes if you want. Can save the rest of the PRP in freezer.

    2. First Spin - Spin your tubes in centrifuge. Pipette the plasma and buffy coat into sterile tubes. If you get a little red blood cells that's fine too, no big deal. Just try to be careful only get plasma and buffy coat. https://en.wikipedia.org/wiki/Buffy_...ion-scheme.png

    3. Second spin - spin your sterile tubes in centrifuge AGAIN. This is to get more concentrated PRP. Ok, now you should see a tube full of yellow goodness and possible a few drops on blood at the bottom. The droplets of blood is from your pipette session if you accidentally sucked in a little red blood cell. like i've said, no big deal. Ok, now look at the tubes, the top 2/3 of it is PPP (Platelet Poor Plasma). Those are not what you want. You only want to keep the bottom 1/3 of the plasma, which is your PRP (platelet RICH plasma). You can research what you can use with the PPP, mostly we just discard it. But I don't know, maybe you can rub on your face? http://image.ec21.com/company/b/bs/b...pimg/01-03.jpg
    So at this point, you will want to pipette out top 2/3 of it and discard. Save the rest of it for your injection. Injecting your PRP with a little droplets of red blood cell is okay.

    4. Injection - Use lidocaine numbing cream if you want. https://www.amazon.com/Lidocane-Plus...lidocaine+4%25. Follow package instructions.

    Get yourself some 1-3ml syringes. Get the needles from here: https://beautygroup-shop.com/meso-re...-12mm-25mm-1pc. Why you asked? because these needles are 4mm deep. You want the PRP to be as near to your hair follicles as possible. Your hair follicles are about 4mm deep from your scalp. This 4mm needles will also prevent your injections to go too shallow or too deep. Some people like to add calcium to "open up" the platelets. You can do that too. But seriously, this step is optional.

    5. Frequency? Well, once every two weeks sounds reasonable to start with. Then you can reduce to once every 4 weeks.

    Good luck!
    Hey woodpecker, any update on your PRP regime? How effective has this been for hair loss?

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