PRP At home

[TLett]

I had that question too! This video answered it and it’s a really simple thing.

https://youtu.be/XAhBzUosvsU

[Gradejohn]

I’ve been researching this online. I bought everything I need to do PRP at home. However I’m curious about doctors who have been saying they’re adding protein and/or calcium gluconate. Would this be beneficial, and if so how can I get this?

I’m also in the USA. Unfortunately I can’t use the 4 mm needles that attach to syringes because I cannot get them where I am. At least I can’t figure out where I can get them. I’m working on getting a diabetic insulin pen and adapting that. I would love VLOW 3Dose pen.

[Noked]

Ok, just thought I'll put in my 2 cents. Links are just for references, I'm sure you can find something cheaper online or locally.

Drawing blood - I'm a phlebotomist. So this I know A LOT. get a butterfly needle, 21g or 23g. Either draw yourself or get someone to help you.
http://www.plcofamerica.com/blog/201...erfly-needles/

Needle holders - http://www.plcofamerica.com/blog/201...erfly-needles/

Tourniquets to help your veins pop and easier to draw: https://www.amazon.com/Physician-Sup...ds=Tourniquets

Tube rack for when you need to keep your tubes upright to pipette the RPR. Also use it to store your unused PRP in freezer (read below): https://www.amazon.com/SEOH-TEST-TUB...ds=tube+holder

Pipettes to remove plasma from your tubes: https://www.amazon.com/Qingsun-Dispo...words=pipettes

Look thru YouTube on how to do a simple blood draw: https://www.youtube.com/watch?v=_w3-gtzvenk

Type of Tubes: Tubes with ACD solutions or simple EDTA works fine too. The trick is to not let the blood clot. Without ACD or EDTA you will only get serum in return and not plasma. That said, I still prefer ACD.

ACD: https://www.amazon.com/Solution-Tube...ords=acd+tubes

Sterile tubes - you need some sterile tubes to keep your PRP before you transfer to your syringes. Also you can use this to storage your unused PRP and keep them in the freezer. Plasma is usable for up to one year if store in freezer. So keep in mind to draw a little more blood each time. When ready to use the frozen PRP, just thaw them in room temperature. https://www.amazon.com/EarthOx-Steri...=sterile+tubes

Centrifuge: https://www.amazon.com/Tangkula-Elec...rds=centrifuge


Ok here are the steps:

1. Draw your blood in ACD tubes. maybe 6-8 tubes if you want. Can save the rest of the PRP in freezer.

2. First Spin - Spin your tubes in centrifuge. Pipette the plasma and buffy coat into sterile tubes. If you get a little red blood cells that's fine too, no big deal. Just try to be careful only get plasma and buffy coat. https://en.wikipedia.org/wiki/Buffy_...ion-scheme.png

3. Second spin - spin your sterile tubes in centrifuge AGAIN. This is to get more concentrated PRP. Ok, now you should see a tube full of yellow goodness and possible a few drops on blood at the bottom. The droplets of blood is from your pipette session if you accidentally sucked in a little red blood cell. like i've said, no big deal. Ok, now look at the tubes, the top 2/3 of it is PPP (Platelet Poor Plasma). Those are not what you want. You only want to keep the bottom 1/3 of the plasma, which is your PRP (platelet RICH plasma). You can research what you can use with the PPP, mostly we just discard it. But I don't know, maybe you can rub on your face? http://image.ec21.com/company/b/bs/b...pimg/01-03.jpg
So at this point, you will want to pipette out top 2/3 of it and discard. Save the rest of it for your injection. Injecting your PRP with a little droplets of red blood cell is okay.

4. Injection - Use lidocaine numbing cream if you want. https://www.amazon.com/Lidocane-Plus...lidocaine+4%25. Follow package instructions.

Get yourself some 1-3ml syringes. Get the needles from here: https://beautygroup-shop.com/meso-re...-12mm-25mm-1pc. Why you asked? because these needles are 4mm deep. You want the PRP to be as near to your hair follicles as possible. Your hair follicles are about 4mm deep from your scalp. This 4mm needles will also prevent your injections to go too shallow or too deep. Some people like to add calcium to "open up" the platelets. You can do that too. But seriously, this step is optional.

5. Frequency? Well, once every two weeks sounds reasonable to start with. Then you can reduce to once every 4 weeks.

Good luck!

Use this as a resource. See above.

[Gradejohn]

Use this as a resource. See above.

Thank you. What about money order to the US

[Gradejohn]

I should not have attempted to use voice to text. That should have read, it will not let me order to the US.

[Gradejohn]

Well, I just finished my first home PRP treatment. To me the hardest part was using the pipettes to cleanly get the plasma and Buffy coat out of the tubes after the first spin. I got more red blood cells that I wanted, but was still concerned I was missing some Buffy coat. I will keep everyone informed of my progress.

Also, here’s hoping it doesn’t cause too much shed.

[TLett]

Well, I just finished my first home PRP treatment. To me the hardest part was using the pipettes to cleanly get the plasma and Buffy coat out of the tubes after the first spin. I got more red blood cells that I wanted, but was still concerned I was missing some Buffy coat. I will keep everyone informed of my progress.

Also, here’s hoping it doesn’t cause too much shed.

Hoping for your success! Please do keep us posted really really interested because I too am on the verge of doing this. Questions friend: did you have any trouble with your self draw? Did someone help you with it? Hand or arm? If I’m right handed, should I draw from my left arm? Thx in advance!!!

[Gradejohn]

Hoping for your success! Please do keep us posted really really interested because I too am on the verge of doing this. Questions friend: did you have any trouble with your self draw? Did someone help you with it? Hand or arm? If I’m right handed, should I draw from my left arm? Thx in advance!!!

Thankfully one of my best friends is a head nurse at a hospital. That said, I was not above doing it myself. The trouble we had is the butterfly needle that I found. The gauge was too small and my blood clot it after the second tube of blood. So we have to find a larger gauge butterfly or surroun thankfully one of my best friends is a head nurse at the hospital. That said, I was not above doing it myself. The trouble we had is the butterfly needle that I found. The gauge was too small and my blood clotted after the second tube of blood. After that we use the syringe on my other arm to draw blood.

So we have to find a larger gauge butterfly or syringe. This was a bit disappointing to me.

[Gradejohn]

Also, on my first spin in the centrifuge, I spun for 3 1/2 minutes at 3000 RPM. On my second spin, I spun for 4000 RPM at 15 minutes. If someone has a better suggestion, I would be happy to hear it.

[TLett]

Thx so much for your answers! I’ve watched several YouTube videos on how to do a self draw and I’m not afraid. And I really appreciate your info on the spin specs you used. That part has confused me a bit. I see that your centrifuge isn’t expensive, if you purchased the one on Noked list of items. Do you feel like this spinner is sufficient for the use we’re using it for? Man you can spend as much money as you want to on those machines geez 🤔