PRP At home

[unknowndude]

This topic is intriguing to me. I was looking around the web and found this DrPRP site. It's a medical company that sells kits to separate PRP and plasma from blood. They claim the kit can help separate and reclaim the pure PRP and gets a 97% recovery rate. $180 for a seemingly simple plastic device seems steep but might be worth it for those who are hardcore about this. The kit wasn't as interesting as the instructions on their site.

https://drprpusa.com/product/prp-kit-sample/

The experts who have done research and are already self-applying PRP might want to look that over.

BTW, They recommend 3000 RPM centrifuge speed and a 4 minute first spin. 3200 RPM and 6 minute 2nd spin. They recommend larger needles and counterbalancing the PRP tube with another tube on the opposite side of the centrifuge.

Hope this helps someone. I want to see more feedback here before I bite the bullet and try this personally. Would love for people who are doing this to check back in frequently!

[Sensorium]

What a great thread. I just picked up 10 of the 3dose syringes that Birdman recommended. I’ll also be using the 4mm needles recommended. Can anyone comment on the number of injections and dose per injection? Is there a certain number of units we are to be injecting every cm2? Or can they be more spread out? Also, if you are using a 4mm needle, I assume you inject straight in (no angle)? How often to do you do every 2 weeks before switching to once a month?

I just had a Rahal transplant with PRP and I’d like to do the follow up PRP doses at home.

Thanks!

[SuperDPAsteve]

Like another poster said, this is very intriguing and I think I’ll try it out myself. I’m collecting a shopping cart of supplies online. Big thanks to OP and birdman for the info.

The two things I’m still fuzzy on: time and speed in the centrifuge. Is this an exact science or more of a “within a certain range” type of thing? Can you tell by looking at the tube when to stop?

Second - once you’ve finished your second spin and have the PRP ready to go, how do you go around getting it in the syringe? 4mm isn’t gonna be long enough to reach down in that tube to the PRP

Thanks again for all the informative posts

[Gabryhell]

Hi guys i'm new to the forum and i'm very interested in this threads. I bought all i need for this "homemade prp" But maybe something is missing... Do i need to activate the plasma with some chemicals? I feel like a crazy scientist with this stuff around 🤣. Thanks in advance for the answer and sorry if my english seems a bit off.

[DougS]

Hi Gabryhell, I am new to the thread too and also like you I just bought everything I need for home prp. The last delivery of BD tubes with acd-a is coming in on Tuesday. I was thinking about that same question. I don't we need to activate it. I found this study on Pubmed https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343942/ I haven't finished reading it, yet. I found another study that said that to avoid early activation to use a needle to withdraw blood greater than 22 gauge because thinner needles will activate the platellets. And the hypodermic needle I am going to use is 31 guage. So I think that means that the 31G will activate it. I am still reading.

[Gabryhell]

Hi Gabryhell, I am new to the thread too and also like you I just bought everything I need for home prp. The last delivery of BD tubes with acd-a is coming in on Tuesday. I was thinking about that same question. I don't we need to activate it. I found this study on Pubmed https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5343942/ I haven't finished reading it, yet. I found another study that said that to avoid early activation to use a needle to withdraw blood greater than 22 gauge because thinner needles will activate the platellets. And the hypodermic needle I am going to use is 31 guage. So I think that means that the 31G will activate it. I am still reading.

Hi doug, thanks for this material But now i have even more questions XD the problem is that i wanted to use a dermaroller, But maybe i will buy a needle instead. Anyway i'm from Italy and i didn't find any tube with acd-a bit i bought 100 with sodium citrate i hope it Will work as good.

[DougS]

ACD stands for Acid Citrate Dextrose and ACD-A/ACD-B are the different mixtures. I found this long blog (https://www.researchgate.net/post/Wh...trate_or_ACD-A) among many doctors on this topic. They say that ACD-A is best. About 3/4 down the page a Max-Joseph Kraus from Universität Koblenz-Landau gave a lot of details including the product's catalog number (367714) of the tube with ACD-A that he uses. Its made by BD and I found their product catalog (https://www.bd.com/resource.aspx?idx=30770) and the product is at the bottom of page 21. I bought mine here (http://www.pulmolab.com/product_info...0wVVQFsKKHvOCd). Its arriving on Tuesday.

[DougS]

I think that sodium citrate may be different that Citrate Dextrose.

[Peeone78]

I have been doing my own "at home" PRP as well and also have all of the necessary equipment. However, it seems this thread has not gotten much attention, though.

Strange?

-Birdman

Hi birdman where did you get your calcium chloride from

[DougS]

Ok, just thought I'll put in my 2 cents. Links are just for references, I'm sure you can find something cheaper online or locally.

Drawing blood - I'm a phlebotomist. So this I know A LOT. get a butterfly needle, 21g or 23g. Either draw yourself or get someone to help you.
http://www.plcofamerica.com/blog/201...erfly-needles/

Needle holders - http://www.plcofamerica.com/blog/201...erfly-needles/

Tourniquets to help your veins pop and easier to draw: https://www.amazon.com/Physician-Sup...ds=Tourniquets

Tube rack for when you need to keep your tubes upright to pipette the RPR. Also use it to store your unused PRP in freezer (read below): https://www.amazon.com/SEOH-TEST-TUB...ds=tube+holder

Pipettes to remove plasma from your tubes: https://www.amazon.com/Qingsun-Dispo...words=pipettes

Look thru YouTube on how to do a simple blood draw: https://www.youtube.com/watch?v=_w3-gtzvenk

Type of Tubes: Tubes with ACD solutions or simple EDTA works fine too. The trick is to not let the blood clot. Without ACD or EDTA you will only get serum in return and not plasma. That said, I still prefer ACD.

ACD: https://www.amazon.com/Solution-Tube...ords=acd+tubes

Sterile tubes - you need some sterile tubes to keep your PRP before you transfer to your syringes. Also you can use this to storage your unused PRP and keep them in the freezer. Plasma is usable for up to one year if store in freezer. So keep in mind to draw a little more blood each time. When ready to use the frozen PRP, just thaw them in room temperature. https://www.amazon.com/EarthOx-Steri...=sterile+tubes

Centrifuge: https://www.amazon.com/Tangkula-Elec...rds=centrifuge


Ok here are the steps:

1. Draw your blood in ACD tubes. maybe 6-8 tubes if you want. Can save the rest of the PRP in freezer.

2. First Spin - Spin your tubes in centrifuge. Pipette the plasma and buffy coat into sterile tubes. If you get a little red blood cells that's fine too, no big deal. Just try to be careful only get plasma and buffy coat. https://en.wikipedia.org/wiki/Buffy_...ion-scheme.png

3. Second spin - spin your sterile tubes in centrifuge AGAIN. This is to get more concentrated PRP. Ok, now you should see a tube full of yellow goodness and possible a few drops on blood at the bottom. The droplets of blood is from your pipette session if you accidentally sucked in a little red blood cell. like i've said, no big deal. Ok, now look at the tubes, the top 2/3 of it is PPP (Platelet Poor Plasma). Those are not what you want. You only want to keep the bottom 1/3 of the plasma, which is your PRP (platelet RICH plasma). You can research what you can use with the PPP, mostly we just discard it. But I don't know, maybe you can rub on your face? http://image.ec21.com/company/b/bs/b...pimg/01-03.jpg
So at this point, you will want to pipette out top 2/3 of it and discard. Save the rest of it for your injection. Injecting your PRP with a little droplets of red blood cell is okay.

4. Injection - Use lidocaine numbing cream if you want. https://www.amazon.com/Lidocane-Plus...lidocaine+4%25. Follow package instructions.

Get yourself some 1-3ml syringes. Get the needles from here: https://beautygroup-shop.com/meso-re...-12mm-25mm-1pc. Why you asked? because these needles are 4mm deep. You want the PRP to be as near to your hair follicles as possible. Your hair follicles are about 4mm deep from your scalp. This 4mm needles will also prevent your injections to go too shallow or too deep. Some people like to add calcium to "open up" the platelets. You can do that too. But seriously, this step is optional.

5. Frequency? Well, once every two weeks sounds reasonable to start with. Then you can reduce to once every 4 weeks.

Good luck!

That was helpful, but I did find one thing on Pubmed that said that "Lidocaine had a negative effect on platelet aggregation rate". I think that might be why some places don't use anaesthetics for PRP hair injections. Here the research link https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4049644/