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Fasting can add a range of beneficial "regenerative" elements to the blood. Just thought I'd toss that into to mix for discussion. From my understanding, even intermittent fasting initiates apoptosis (cell death) to kill off old, weak cells and replaces them with healthy new ones. If the cell is no longer insulin sensitive, like a lot of people are these days from too much sugar/carbs, then I'm guessing those are the same people who I've read are less impressed with their person experience with PRP as compared to others who see great results and who probably have a better diet. I agree that it's best to use your own blood if possible, but I've been looking into stem cells and it seems if someone had a pretty bad illness, they need to find a healthy source for best result. Just throwing out another perspective to consider. Great discussion!
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PRP Tips
Hi All,
Ive been looking over peer review research on the efficacy of PRP for hairloss, and I can tell you - it's very strongly supported. If done well, it can lead to a 35% increase in hair density.
I have been looking into the method that would yield the highest PRP concentration. Dhurat & Sukesh (2014) suggested that 1st spin at 900g and second at 1000g yields the best results.
As every centrifuge is different (and has a different diameter between tubes), you should calculate the RPM's necessary at each of the stages using the calculation available at this website: http://insilico.ehu.es/mini_tools/rcf_rpm.php. The radius is the distance between the bottom of the tube and the center of the rotator.
A few other tips:
1) when drawing blood, use a large needle (>22g). This will fasten up the process and limit the amount of blood clotting that occurs.
2) After blood draw, invert tubes 5-10 times for proper mixing of the anticoagulant and blood.
3)For highest yield results, centrifuge at 16 degrees C. For me, this is unrealistic, but i'lll try to reduce the temp to room temp.
4) after second spin, seperate the PRP from the PPP relatively quickly. With time, the PRP will slowly diffuse into the PPP.
5) Activation of PRP does not seem to provide any advantages.
Hope this helps. Good luck.
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Hello, i wonder if i can use this tube for prp? thanks for answer
https://www.fishersci.ca/shop/produc...ator/p-6962057
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No that vacutainer is not the right one. You want the vacutainer with a yellow top. This is the one I bought, link below. Yellow has the ACD-A anticoagolant. Some people use the vacutainer with EDTA but ACD-A is better. I got that info from a lengthy blog among doctors at this link (https://www.researchgate.net/post/Wh...trate_or_ACD-A)
https://www.pulmolab.com/product_inf...RoCv7MQAvD_BwE
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Great conversation. I was wondering if there are other people that are self-administrating PRP therapy and I am kind of relieved they are.
I initially started injected my neck (I have a chronic issue), but eventually got deep into the general regenerative purposes.
Do you guys use prp therapy for specific physical issues only or for general prevention?
Originally Posted by hairloser94
I do not think that will do. I buy mine from here - https://prp-plasmolifting.com/produc...plasmolifting/ as it is the best price I have found and the quality is great. No difference with the 200 usd kit I bought the first time.
What centrifuges are you guys using? Any that are far better and preferable than than the rest?
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I only use PRP for hair treatment. The centrifuge I use is an older "Clay Adams Dynac" (link below) with a swing gate rotor that I bought on eBay for $190 including shipping. I paid a little extra so that I could get the swing gate rotor. The advantage of using a swing gate rotor is that the layers are perpendicular to the centrifugal force and lay flat in the tube. With non swing gate rotors the layers will be angled and there is also a slight remixing as the centrifuge slows down
http://www.blockscientific.com/manua...centrifuge.pdf
Last edited by DougS; 03-29-2019 at 11:07 AM.
Reason: mispelled word
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I see a lot of people have had issues injecting the plasma into the scalp. How about using an injecting gun like this one??
https://www.ebay.com/itm/High-Pressu...EAAOSwmBxa3uoD
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Originally Posted by dezertfox
It would definitely be easier. I also wonder about injecting directly into the scalp. I have a friend that is doing it, but she openly complains it is notoriously difficult.
On a side note...Has anyone tried Dermaheal? I read good things about it, but it might be yet another hair loss solution that does little to nothing - https://medisilk.com/dermaheal-hl-5-x-10-vials/
Price is not too steep. It is injected directly into the scalp as well. Has anyone even heard of it?
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Originally Posted by Semtex7
It would definitely be easier. I also wonder about injecting directly into the scalp. I have a friend that is doing it, but she openly complains it is notoriously difficult.
On a side note...Has anyone tried Dermaheal? I read good things about it, but it might be yet another hair loss solution that does little to nothing - https://medisilk.com/dermaheal-hl-5-x-10-vials/
Price is not too steep. It is injected directly into the scalp as well. Has anyone even heard of it?
I am inclined to try it since 150 bucks or so, but can't find much anecdotes on the internet, can you point me to some?
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Originally Posted by leonard
Birdman and Zinkin, thank you for posting your experiences with at home PRP, it has been very helpful and motivating.
I have purchased my own centrifuge and currently working on finding the anticoagulant.
Is there an in depth tutorial on how to do this? I have been looking for a step by step guide and materials needed list and have not found anything.
I have just pieced together the process from various sources, but would appreciate your help in getting it done right the first time.
thanks again.
Leonard, how was your experience with PRP then?
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