Dr. Wesley has begun his 3rd 'Pilofocus' Phase Testing- I was finally involved!

**tedwuji** · 02-11-2017, 09:53 PM

Originally posted by long4hair

I'll check back in 6 months.

I came here every day during November so I could see if you posted anything about your November appointment with Dr. Wesley and then you told us you cxld at the last minute and you were rescheduled for January.

I came here every day during January so I could see if you posted anything about your January appointment with Dr. Wesley and then you told us you cxld at the last minute.

Now you're saying you're going to see Wesley in March or April and I don't want to spend either of those 2 months here waiting with baited breath for news when I'm so uncertain you'll go to the appointment.

I agree with this, too.

**Arashi** · 02-12-2017, 05:09 PM

LOL this thread is hilarious.

Come on people, hair regeneration does not exist. The whole hair regeneration hype was started by HASCI. Check out the analysis thread, it's 100% fake. No regeneration happens at HASCI. Then Dr Nigam came, we all know how it ended with that fraudster. Then some Dr from France, don't even remember his name, but he tried it and failed. Then we had Dr Mwamba, he went dead silent. And now this hilarious thread, full of promises and ... well, more promises

And all this because HASCI claimed to be able to do it

Hair regeneration by splitting follicles is a dead end, we can finally draw that conclusion now. Just move on, until someone finds out how to duplicate DP cells we're stuck with FUE + Fin + minox

**tedwuji** · 02-12-2017, 09:34 PM

Originally posted by Arashi

LOL this thread is hilarious.

Come on people, hair regeneration does not exist. The whole hair regeneration hype was started by HASCI. Check out the analysis thread, it's 100% fake. No regeneration happens at HASCI. Then Dr Nigam came, we all know how it ended with that fraudster. Then some Dr from France, don't even remember his name, but he tried it and failed. Then we had Dr Mwamba, he went dead silent. And now this hilarious thread, full of promises and ... well, more promises

And all this because HASCI claimed to be able to do it

Hair regeneration by splitting follicles is a dead end, we can finally draw that conclusion now. Just move on, until someone finds out how to duplicate DP cells we're stuck with FUE + Fin + minox

Im not here for regen.

I am here for a scarless transplant using an endoscope.

**long4hair** · 02-12-2017, 09:57 PM

Originally posted by Arashi

LOL this thread is hilarious.

Come on people, hair regeneration does not exist. The whole hair regeneration hype was started by HASCI. Check out the analysis thread, it's 100% fake. No regeneration happens at HASCI. Then Dr Nigam came, we all know how it ended with that fraudster. Then some Dr from France, don't even remember his name, but he tried it and failed. Then we had Dr Mwamba, he went dead silent. And now this hilarious thread, full of promises and ... well, more promises

And all this because HASCI claimed to be able to do it

Hair regeneration by splitting follicles is a dead end, we can finally draw that conclusion now. Just move on, until someone finds out how to duplicate DP cells we're stuck with FUE + Fin + minox

What about this?

Hair regeneration from transected follicles in duplicative surgery: rate of success and cell populations involved - PubMed

https://www.ncbi.nlm.nih.gov/pubmed/19438685

Duplicative surgery might represent a successful alternative for hair transplantation, because both portions are capable of regenerating a healthy hair. Moreover, our results suggest the possible presence of stem cells in both halves of the follicle.

**long4hair** · 02-12-2017, 10:06 PM

Originally posted by Arashi

Just move on, until someone finds out how to duplicate DP cells we're stuck with FUE + Fin + minox

Would the technique in these 2 studies solve the inductivity problem?

Increasing the hair inductive potential of human dermal papilla cells: stimulating and characterising cell aggregation

https://minerva-access.unimelb.edu.au/handle/11343/55427

Dermal papilla cells (DPCs) are able to induce hair follicles. DPC tend to aggregate both in vitro and in vivo. This tendency is associated with their ability to induce hair growth. The use of DPCs to treat alopecia is limited because human DPCs lose their hair-inducing activity in culture, whereas ovine DPC do not. The aims of this study were to characterise the molecular phenotype of ovine DPC aggregates, and to determine whether aggregating ovine DPCs secrete factors affecting the aggregative behaviour or inductive potential of human DPCs. Expression of papilla markers in cultured ovine DPCs was characterised. The effects of ovine factors, different culture substrates and medium compositions on aggregative behaviour of human DPCs were determined. Co-cultures of ovine and human papilla cells, separated by a permeable membrane were observed to determine whether the ovine cells secrete soluble factors that affect human cell aggregation. Ovine DPC aggregates expressed 16 papilla markers, showing they have a similar phenotype to papillae in vivo. In co-culture experiments, well-formed aggregates were produced in human:ovine DPC mixtures. In contrast, unmixed human DPCs remained in a monolayer state, indicating that ovine cells are required to initiate aggregation but the human cells are then able to incorporate into aggregates. Both human and ovine DPCs had a higher tendency to aggregate in medium containing 20% (v/v) lamb serum compared to 10% (v/v) foetal calf serum. The effect of co-culturing human with ovine DPCs separated by a permeable membrane gave positive additional effects to human aggregation. In summary, ovine biomolecules show potential for increasing the aggregative behaviour of human DPCs in culture. These biomolecules might eventually be used to treat androgenetic alopecia.

The Effect of Ovine Secreted Soluble Factors on Human Dermal Papilla Cell Aggregation - PubMed

https://www.ncbi.nlm.nih.gov/pubmed/27625561

Soluble factors secreted by ovine DPC and present in LS increase the aggregative behavior of human DPC. These molecules might improve follicle inductiveness of human DPCs for the purpose of hair replacement therapy.

If this would protect enough inductivity then why don't we just use this technique now?

**Arashi** · 02-13-2017, 05:36 AM

Originally posted by long4hair

What about this?

https://www.ncbi.nlm.nih.gov/pubmed/19438685

Yeah I've seen that study in the past. I'm not sure what to think of it. One explanation might be that it's fake, like there are so many fake research articles out there. Another explanation might be that it actually works when you split the follicle under a microscope, like they've done in this study. I remember dr Gardner, who I hold very high, said he thought that it would be theoretically possible too, to dissect the follicle under a microscope, get the DP cells and make 2 new hairs that way.

I haven't read the whole study though, did they measure hair diameter ? If the resulting hair is 50% thinner then it's still not something really interesting.

Anyway, even if it would work, it's just not feasible, at least not by hand, it would take weeks of work to split like 3000 follicles, I think dr Gardner said about 3 minutes per follicle to dissect and split the DP cells, so 20 follicles an hour, 160 a day, 18 days for 1 patient.

**long4hair** · 02-13-2017, 07:19 PM

Arishi you didn't say what you think of this technology. Does it solve the inductivity problem?

Increasing the hair inductive potential of human dermal papilla cells: stimulating and characterising cell aggregation

https://minerva-access.unimelb.edu.au/handle/11343/55427

Dermal papilla cells (DPCs) are able to induce hair follicles. DPC tend to aggregate both in vitro and in vivo. This tendency is associated with their ability to induce hair growth. The use of DPCs to treat alopecia is limited because human DPCs lose their hair-inducing activity in culture, whereas ovine DPC do not. The aims of this study were to characterise the molecular phenotype of ovine DPC aggregates, and to determine whether aggregating ovine DPCs secrete factors affecting the aggregative behaviour or inductive potential of human DPCs. Expression of papilla markers in cultured ovine DPCs was characterised. The effects of ovine factors, different culture substrates and medium compositions on aggregative behaviour of human DPCs were determined. Co-cultures of ovine and human papilla cells, separated by a permeable membrane were observed to determine whether the ovine cells secrete soluble factors that affect human cell aggregation. Ovine DPC aggregates expressed 16 papilla markers, showing they have a similar phenotype to papillae in vivo. In co-culture experiments, well-formed aggregates were produced in human:ovine DPC mixtures. In contrast, unmixed human DPCs remained in a monolayer state, indicating that ovine cells are required to initiate aggregation but the human cells are then able to incorporate into aggregates. Both human and ovine DPCs had a higher tendency to aggregate in medium containing 20% (v/v) lamb serum compared to 10% (v/v) foetal calf serum. The effect of co-culturing human with ovine DPCs separated by a permeable membrane gave positive additional effects to human aggregation. In summary, ovine biomolecules show potential for increasing the aggregative behaviour of human DPCs in culture. These biomolecules might eventually be used to treat androgenetic alopecia.

The Effect of Ovine Secreted Soluble Factors on Human Dermal Papilla Cell Aggregation - PubMed

https://www.ncbi.nlm.nih.gov/pubmed/27625561

Soluble factors secreted by ovine DPC and present in LS increase the aggregative behavior of human DPC. These molecules might improve follicle inductiveness of human DPCs for the purpose of hair replacement therapy.

**Artista** · 02-21-2017, 10:23 AM

Hello everybody, Artista here!!

I called this morning to Dr. Wesley's office 30 minutes ago to speak again with 'Billena',
Dr Wesley's Doctor Representative!

We are definitely going to set out my Phase Test Update for next month in March!
'Billena' had wrote on BTT that they truly need me to finally get back there to get the medical verification and update on my Pilofocus hair regeneration Phase Test.

**Artista** · 02-21-2017, 10:24 AM

Hello everybody, Artista here!!

I called this morning to Dr. Wesley's office 30 minutes ago to speak again with 'Billena',
Dr Wesley's Doctor Representative!

We are definitely going to set out my Phase Test Update for next month in March!
'Billena' had wrote on BTT that they truly need me to finally get back there to get the medical verification and update on my Pilofocus hair regeneration Phase Test.

**Artista** · 02-21-2017, 03:43 PM

Again,
For quite a while, I had serious health issues,
(i'm relatively OK now) and I was "overworked" at where I am at, at my regular job !
Also my aunt had died recently !!! I was so sad along with my family members!!
That is why I have not been able to do the things here that I normally can do !!
Everything is getting better for me now and next month definitely am going to Dr. Wesley's PiloFocus Clinic in
New York City.
Hopefully, I will finally meet up with 'Joe From Staten' after I'm done at PiloFocus !
I know his full name but I cannot share it here.
He has serious emotional issues right now.
I truly hope to meet with him and talk in person.
I'm at work right now and I have to go.
Cheers ALL

**Artista** · 03-03-2017, 07:01 AM

HELLO EVERYBODY!

I had contacted Dr.Wesley's Pilofocus office the last week of February 2017.
I have been scheduled to meet-up with Dr. Wesley this month for the update in regards to my
'Pilofocus Hair Regeneration' Phase Test.
He will be able to medically verify my donor area that was used for the phase test.

**FearTheLoss** · 03-03-2017, 12:22 PM

Artista, when is your appointment so we know when to check back? Best of luck man!

**Artista** · 03-03-2017, 01:18 PM

It's on the third week of this month
Thanks for asking "Fear'"

**Artista** · 03-03-2017, 01:19 PM

To let you know , i'm at work now so I couldn't really respond fast

**garethbale** · 03-03-2017, 05:11 PM

I think he meant 'what date is your appointment'....

Dr. Wesley has begun his 3rd 'Pilofocus' Phase Testing- I was finally involved!

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