Masayuki Amagai-Professor, Department of Dermatology, Graduate School of Medicine, Ke

[Renee]

I just read this at dr Amagai's university web page:

Characterization of human hair follicles

We identified the cell surface marker of stem cell-enriched human bulge cells and, using this marker, successfully isolated and cultured living human bulge cells (Ohyama et al., J Clin Invest, 2006). We also established the culture conditions for sustained expression of genes related to trichogenic activity in human dermal papilla cells (patent filed). In-vivo assays and 3D cultures for hair follicle reconstitution have been developed. These cultivated cell components and assays will facilitate experiments for the regeneration of hair follicles.

Development of treatment for scarring alopecia using bioengineered human hair follicles

The goal of the present project is to bioengineer human hair follicles with the maintenance of their full function and structure, for potential use in the treatment of permanent hair loss. We shall attempt to regenerate human hair follicles using a cell mixture of hair follicle bulge stem cells and dermal papilla cells with optimized hair-inductive capacity. The hair reorganization potency of the cell mixture will be assessed by hair reconstitution assays in vivo or using a 3-D culture system.

http://www.med.keio.ac.jp/gcoe-stemcell/english/member/amagai.html

Look at figure 2: Fig.2 Regeneration of human hair follicle with cultured human dermal papilla cells inmice

It shows a lot of regrowth.

[champpy]

Is that human hair growing from a human piece of skin grafted onto a mouse? Or human hair growing from mouse skin? Id be much more impressed if its sprouting from human skin

Looks like they still havent solved the issue of hair pigmentation

[nameless]

I just read this at dr Amagai's university web page:

Characterization of human hair follicles

We identified the cell surface marker of stem cell-enriched human bulge cells and, using this marker, successfully isolated and cultured living human bulge cells (Ohyama et al., J Clin Invest, 2006). We also established the culture conditions for sustained expression of genes related to trichogenic activity in human dermal papilla cells (patent filed). In-vivo assays and 3D cultures for hair follicle reconstitution have been developed. These cultivated cell components and assays will facilitate experiments for the regeneration of hair follicles.

Development of treatment for scarring alopecia using bioengineered human hair follicles

The goal of the present project is to bioengineer human hair follicles with the maintenance of their full function and structure, for potential use in the treatment of permanent hair loss. We shall attempt to regenerate human hair follicles using a cell mixture of hair follicle bulge stem cells and dermal papilla cells with optimized hair-inductive capacity. The hair reorganization potency of the cell mixture will be assessed by hair reconstitution assays in vivo or using a 3-D culture system.

http://www.med.keio.ac.jp/gcoe-stemcell/english/member/amagai.html

Look at figure 2: Fig.2 Regeneration of human hair follicle with cultured human dermal papilla cells inmice

It shows a lot of regrowth.

This information needs to be gotten into the hands of replicel and any other researchers experimenting with cell implantation to treat hair loss.

[FooFighter]

Yes, but Replicel is testing their method on humans not on rat, lol!

I am not even reading articles where baldness is cured on mice.

[nameless]

Yes, but Replicel is testing their method on humans not on rat, lol!

I am not even reading articles where baldness is cured on mice.

Yea, and we don't know what would happen if Replicel tested this new method (in humans) to preserve trichogenicity, do we?

So it's a good idea to send them this new information and suggest to them that they should consider looking into this new method to protect trichogenicity.

[Renee]

It's significant because they cultured dermal papilla cells that were able to induce hair.

Dr manabu ohyama from shisheido was a co researcher and here is the patent filed from 2010. It's amazing that since then we still have no good treatment.

https://www.google.com/patents/WO2010021245A1?cl=en&dq=Masayuki+Amagai+hair&hl=en&sa=X&ved=0CBsQ6AEwAGoVChMImJLR38fwyAIVCdUeCh2JAAoQ

[lacazette]

Agree it's frustrating Renee, but since that time, the problem is the large scale amount as we need hundreds of these inductive DPs

as you know the more you try to expand the cells, the more it's difficult to make them don't loose their potential, and so the more the protocol has to be powerful

Large scale production is becoming possible with progress in scaffolds, environment factors, cultur methods, (also bioprinting), etc (like the chineses one for example :http://www.ncbi.nlm.nih.gov/pubmed/26118627)

but the problem is that they have to work and found ameliorations on every aspects of the whole process (for cheapness, easiness, safety, time consuming,and everything) for that the all in one protocol become viable commercially


us army researchers explained well in 2014:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057739/

"One limitation of applying these cells for hair regeneration is that expansion and frequent passage of trichogenic cells results in loss of hair inductive potential. Advancements in cell culture technique like using less trypsin during multiplication [106], three dimensional culture systems [107,108], xeno free culture system [109], conditioning with human serum [63,110] or supplementing media with morphogens like wnt and bmp modulators [111–113] can lead to methods for extending the efficacy of these cells."

" It offers a safe, immunologically acceptable and simple alternative for tissue regeneration applications.
Practical challenges including scale up, formulation, storage, transport and delivery mechanisms are the main challenges to development and clinical translation of preclinical observations."

----

theorically the cure is here, they know how to keep inductivity of the DPs to grow new hair, and now they begin to find different ways to make large scale amount with keeping this inductivity

BUT they need to work on every little fcking aspects and enhanced every little things in each domains, to make the whole processus become a simple protocol that is a commercially viable thing (and less expensive than for exemple 100 000 USD)

So for sure every aspects to enhance is quite difficult, that's why it's going damn slowly (though there is big progress on culture methods, scaffolds things, bioprinting,etc and big methods progress in each domains (cheapness, easiness, etc etc), so the in fine cheap protocol is not that far away ( like hopefully the shiseido's one)

and that's why amagai, ohyama, tsuji and others are damn interested by iPSCs, cause with iPSCs you don't need anymore the complicated protocols (the one for culture and expand the cells, and the one even more complicated that you have to add for make these cells keeping their inductivity)

the iPSCs can offer the unlimited amount of inductive DPs we would need, and with them the protocol become cheap, faster, and everything, so commercially viable
BUT safety issue is the problem and has to be definitly determined before any things can be tested

But at least in the worst case where Shiseido or another classic cells culture protocols (or with the wounding way (Lgr6 protocol,follica))don't success to achieve hair regeneration in the coming years, iPSCs will be here to do the job once safety is overcomed ( as you see in the ips topic, safety iPSCs progress is growing insanely as they are needed in any area as they have beneficial potential in every single disease)
let's just hope we will don't need them!

[Renee]

Ips cells are way too dangerous and a long way from being used in a clinical environment. A better solution is using Autologous stem-cell transplantation (culturing and then injecting inductive dermal papilla cells) to cure baldness in the next 5 years.

[goldnt]

So when can i get my hair back? *cries*

[nameless]

Ips cells are way too dangerous and a long way from being used in a clinical environment. A better solution is using Autologous stem-cell transplantation (culturing and then injecting inductive dermal papilla cells) to cure baldness in the next 5 years.

I think they have almost all, if not all, of the safety bugs worked out for Ips cells. I could be wrong but that is what it looks like in Lacazette's many links.

Sorry if I misspelled your name Lacazette.

[FooFighter]

http://www.genengnews.com/gen-news-highlights/gene-found-that-regulates-stem-cell-number-production/81251921/

[lacazette]

Also regarding Keio Univ, there's this from Keio Hospital clinical research list :

https://translate.google.com/translate?hl=en&sl=ja&tl=en&u=http%3A%2F%2Fwww.derma.med.keio.ac.jp%2Fderma%2Findex.html

-Approval Number:20050075
-Type of research: Challenges name: Basic Research: Attempt human hair 嚢再 producing with hair follicle-derived cells isolated from human scalp tissue
-Research Period: 2006 / 1-2018 / 3
-Principal investigator,Practice responsible: Yamagami Atsushi, Ise Misaki


And there's also this lab from Keio Univ:

http://www.miyata.mech.keio.ac.jp/MIYATALAB_regmed_en.html

"Our field is the area between medical and mechanical engineering. Development of medical technology requires not only medical technique but also mechanical engineering. In Miyata laboratory, we see the medical problem from the viewpoint of mechanical engineers and develop brand new technology for assisting medical technique."

"Cell Patterning for Hair Regeneration

There are many types of alopecia which is diseases of hair growth. Using medicines is popular method for curing hair growth and restoration, but medicines may not available in all cases. If the skin is injured and structure is broken like scarring alopecia, it is impossible to grow the hair even using medicines. To regenerate hair growth ability, reconstruction of skin structure is needed. When making skin structure in vitro, how to place cells is very important. The best way to regenerate same function as living skin is to imitate original structure. To make regenerative medicine of hair realize, we are developing 3D cell patterning devices and trying to make artificial skin structure in vitro. "

[grantwilson]

American Board of Dermatology cooperates with the research center and laboratory teams from universities wordwide. Additionally to the new systemic approaches and planned surveys by essay writing service portal as they are offering unprintable data which may be downloaded from experts. Medical dermatology courses can be also chosen for the second major by international students.

I have some additional materials about new methods of Cell Patterning for Hair Regeneration by Michael Rosenblum (Department of Dermatology, UC Davis). Some of the reports and research papers are published in the Feinberg School of Medicine journals by Maria L Colavincenzo as well.