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Increasing the hair inductive potential of DP cells
SARI, AGNES ROSARINA PRITA is a researcher from melbourne, and here it's her master research thesis:
Increasing the hair inductive potential of human dermal papilla cells: stimulating and characterising cell aggregation
https://minerva-access.unimelb.edu.a...le/11343/55427
Dermal papilla cells (DPCs) are able to induce hair follicles. DPC tend to aggregate both in vitro and in vivo. This tendency is associated with their ability to induce hair growth. The use of DPCs to treat alopecia is limited because human DPCs lose their hair-inducing activity in culture, whereas ovine DPC do not.
The aims of this study were to characterise the molecular phenotype of ovine DPC aggregates, and to determine whether aggregating ovine DPCs secrete factors affecting the aggregative behaviour or inductive potential of human DPCs.
Expression of papilla markers in cultured ovine DPCs was characterised. The effects of ovine factors, different culture substrates and medium compositions on aggregative behaviour of human DPCs were determined. Co-cultures of ovine and human papilla cells, separated by a permeable membrane were observed to determine whether the ovine cells secrete soluble factors that affect human cell aggregation.
Ovine DPC aggregates expressed 16 papilla markers, showing they have a similar phenotype to papillae in vivo. In co-culture experiments, well-formed aggregates were produced in humanvine DPC mixtures. In contrast, unmixed human DPCs remained in a monolayer state, indicating that ovine cells are required to initiate aggregation but the human cells are then able to incorporate into aggregates. Both human and ovine DPCs had a higher tendency to aggregate in medium containing 20% (v/v) lamb serum compared to 10% (v/v) foetal calf serum. The effect of co-culturing human with ovine DPCs separated by a permeable membrane gave positive additional effects to human aggregation.
In summary, ovine biomolecules show potential for increasing the aggregative behaviour of human DPCs in culture. These biomolecules might eventually be used to treat androgenetic alopecia.
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So apparently her biovine molecules could help to culture inductive DP cells for a cellular therapy, but could also eventually be used directly as a medication for existing hair
I saw also that she was one of the travel grant recipients from the 2014 World Congress Hair Research
http://www.hair2014.org/Contents.asp...14&openpage=14
Her work seems quite interesting but I don't find her contact info, would like to ask what she is doin now, if she had link with a company or what,etc.. so if someone find it
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This is interesting but they can't inject ovine cells into humans because doing so would trigger an immune response.
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Senior Member
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She is the media and communications officer on whatever she does anyway. So could be a good shout getting a reply
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Now, do we need to understand the hair loss mechanism to find the cure ?
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Originally Posted by baldybald
Now, do we need to understand the hair loss mechanism to find the cure ?
Can't solve the problem without knowing what the problem is
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@nameless, hey dude it'q not injecting ovine cells but as it is saying : co-culturing human with ovine DPCs separated by a permeable membrane ( to keep the inductive potential
@noisette, thanks bro I sent her a msg, though it will go in her 'other' category of msgs, Im afraid she will not see it
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In this study from the Us army researchers, thampagazam and darling, in 2014 , they explained where we were about a DPs therapy:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4057739/
Cells with the potential for hair follicle neogenesis can be either injected directly in the bald scalp or in areas where hair follicles are desired or can be incorporated in skin substitutes to regenerate skin appendages. One limitation of applying these cells for hair regeneration is that expansion and frequent passage of trichogenic cells results in loss of hair inductive potential. Advancements in cell culture technique like using less trypsin during multiplication [106], three dimensional culture systems [107,108], xeno free culture system [109], conditioning with human serum [63,110] or supplementing media with morphogens like wnt and bmp modulators [111–113] can lead to methods for extending the efficacy of these cells.
We used the reconstitution assay [114,115] with minor modifications to evaluate whether the trichogenic potential of DP cells can be maintained by using three dimensional culture systems.
The resultant HFs contained hair shafts and sebaceous glands (Figure 4A,B), similar to the results of others
We have also recently demonstrated for the first time that isolated, cultured and dissociated human DP cells can induce complete human hair follicles when combined with neonatal foreskin keratinocytes and grafted onto a nude mice [116].
Cellular therapy based on autologous dermal fibroblast holds enormous promise to the field of regeneration medicine. It offers a safe, immunologically acceptable and simple alternative for tissue regeneration applications.
Practical challenges including scale up, formulation, storage, transport and delivery mechanisms are the main challenges to development and clinical translation of preclinical observations. However, recent FDA approval of fibroblast therapy applications for nasolabial folds and the expanding pipeline of other fibroblast therapies in clinical development show that cellular therapy is an expanding field.
scale up, formulation, storage, transport and delivery mechanisms" there are tremendous progress in each kind of theses challenges nowadays. We are more in a technology.practical waiting game for us now, more than a knowledge wiating game about DP indution,etc
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Invite her to the forum, maybe she can discuss some questions
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