Induce hair follicle neogenesis without the help of inductive HF dermal papilla cells

[lacazette]

Sung jan lin is back with good stuff

He had been invited for a presentation in a seminar by the National Institute of Biological Sciences in China
http://webcache.googleusercontent.co...&ct=clnk&gl=us

20 August 2015
Speaker:
Sung-Jan Lin, MD, Ph.D.
Topic:
Inducing hair follicle organogenesis with defined protein factors

Abstract:

Neogenesis of the miniorgan of hair follicles depends on the initiation and perpetuation of cross-talk between keratinocytes and dermal cells. We ask whether this epithelial-mesenchymal interaction can be initiated without introducing inductive mesenchymal cells in postnatal life. We found that protein extract from embryonic skin of specific developmental stage was able to induce hair follicle neogenesis both in a full thickness wound and a modified patch assay in mice without the help of inductive hair follicle dermal papilla cells or newborn dermal cells. Hair follicle neogenesis here was mediated mainly through the effect on fibroblasts. When adult fibroblasts, but not keratinocytes, were cultured with the protein extract, they were conferred the ability to induce new hair follicles. Through phosphoproteomic analysis with mass spectrometry, we found that insulin/IGF signaling was activated and required for the hair follicle inductivity in adult fibroblasts. Finally, we searched for key protein factors to elicit hair follicle neogenesis. Through proteomics analysis with mass spectrometry, we identified 3 extracellular proteins enriched in embryonic skin that together were required and sufficient to induce hair follicle neogenesis in vivo. Therefore, organ regeneration could be initiated by creating a pro-regeneration environment with defined extracellular factors. Identification of such environmental signals can be incorporated with other approaches to enhance tissue regeneration.

damn HF neogenesis in a full thickness wound without DP cells or newborn dermal cells, it sounds huge!

[lacazette]

And now from a few days ago:
http://otl.sinica.edu.tw/en/index.ph...article_id=858

Home -> Technologies Available

Preparation of skin extract for hair follicle neogenesis

Docket Number：03A-1031215

Inventor：Sung-Jan Lin, Mai-Yi Fan, Yu-Ju Chen, Chia-Feng Tsai

IP Status：US pending, TW pending

Abstract：

When skin is wounded, it tends to repair with fibrosis without regeneration of associated appendages. To regenerate functional skin, we hypothesized that skin appendage regeneration can be facilitated by defined extracellular factors. We screened and successfully identified protein extract and a ****tail of specific protein mixtures that are able to induce hair follicle neogenesis.

Fields of Application：

Protein extract or ****tail of specific proteins can be used in (A) As a drug for local administration to induce hair follicle neogenesis; (2) Preparation of bioartificial dermis or bioartificial skin: addition of the extract in bioartificial skin or bioartificial dermis can induce hair follicle neogenesis; (3) In vitro cell culture: addition of this recipe to cultured fibroblasts can confer the fibroblasts trichogenic capability.

Advantages when compared to the existing technologies：

With this technology, hair follicle neogenesis can be induced without expansion of hair follicle dermal papilla cells from the patients. The cost can be reduced.

[allTheGoodNamesAreTaken]

Another good find, but... mice.

[baldybald]

Another good find, but... mice.

And you may hear mice in the next 15 yeas, sorry.

[Mpbman]

Sometimes I wish I was a balding mouse

[lacazette]

it's been a decade that researchers are working to overcomed the large expansion of DP cells and maintenance of their hair-inductive capability (why aderans crashed)

Now sung jan lin is able to induce HF without DP cells! Just with fibroblasts cultured in the right protein extract. If it's the case it's a game changer

And remember this SJ Lin trial in taiwan hospital with 400 people beginned in 2006 ( estimation date 2016)
https://clinicaltrials.gov/ct2/show/...ollicle&rank=3

"This study is to try to maintain cultured dermal papilla cells in spherical structure in vitro before transplanting into dermis in vivo. Also, this study is aimed in clarifying actual mechanism of inducing hair follicle by dermal papilla cells."

This guy want to cure hairloss, he dedicated his life to it , more of one decade studies all in the hair follicle area
He is/will plan for sure to test this new technique (without the need of DP cells) soon I think, if it's not already planned.
could be a safely and simply protocol like aderans (not w/ DP but Fibroblasts cultured cells) but with the efficiency? Or a wound neogenesis protocol with direct application of the protein extract as he claim on his invention? I don't know but I believe in this guy, he's a smart little genius since his teens, look at his parcours

[Renee]

Everything is pre clinical research stage.

[hellouser]

it's been a decade that researchers are working to overcomed the large expansion of DP cells and maintenance of their hair-inductive capability (why aderans crashed)

Now sung jan lin is able to induce HF without DP cells! Just with fibroblasts cultured in the right protein extract. If it's the case it's a game changer

And remember this SJ Lin trial in taiwan hospital with 400 people beginned in 2006 ( estimation date 2016)
https://clinicaltrials.gov/ct2/show/...ollicle&rank=3

"This study is to try to maintain cultured dermal papilla cells in spherical structure in vitro before transplanting into dermis in vivo. Also, this study is aimed in clarifying actual mechanism of inducing hair follicle by dermal papilla cells."

This guy want to cure hairloss, he dedicated his life to it , more of one decade studies all in the hair follicle area
He is/will plan for sure to test this new technique (without the need of DP cells) soon I think, if it's not already planned.
could be a safely and simply protocol like aderans (not w/ DP but Fibroblasts cultured cells) but with the efficiency? Or a wound neogenesis protocol with direct application of the protein extract as he claim on his invention? I don't know but I believe in this guy, he's a smart little genius since his teens, look at his parcours

Fibroblasts? Would that be FGF-9? If so, thats been known for a while thanks to Dr. Cotsarelis. Was even talked about on the Spencers radio show with that regular caller of his....joefromstantonisland or whatever.

[lacazette]

Fibroblasts? Would that be FGF-9? If so, thats been known for a while thanks to Dr. Cotsarelis. Was even talked about on the Spencers radio show with that regular caller of his....joefromstantonisland or whatever.

Nope Fibroblasts are cells (that can be taken from our skin and cultured far more easily than DP cells, in larger proportions,etc - if i remember well, i have to look again about that)

FGF9 and others are just growth factors of these cells


"A fibroblast is a type of cell that synthesizes the extracellular matrix and collagen
The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. Fibroblasts secrete the precursors of all the components of the extracellular matrix, primarily the ground substance and a variety of fibers. The composition of the extracellular matrix determines the physical properties of connective tissues.

"Fibroblast growth factors, or FGFs, are a family of growth factors.The FGFs are heparin-binding proteins and interactions with cell-surface-associated heparan sulfate proteoglycans have been shown to be essential for FGF signal transduction"

[nameless]

so now they can produce hair induced DP cells by way of fibroblasts and by way of iPS cells.

This means that the issue of hair inductivity is solved. Now it's just a matter of running these techniques through trials. This should take 3 years in Japan if they don't have to do animal studies.