Acceleration in iPS cells clinical applications!!

[lacazette]

the most important new 21 July 2015:

Scientists develop method to kill iPS-derived cells with high cancer risk
http://ajw.asahi.com/article/sci_tec...AJ201507220030

Researchers said July 21 they have found a way to eliminate cells derived from induced pluripotent stem (iPS) cells that have a high risk of becoming cancerous, a development that could lead to clinical applications of iPS cells.

“It is a totally new method that can remove cells that could turn cancerous,” said Kenichiro Kosai, a professor of gene therapy and regenerative medicine at Kagoshima University. “The technique could be used for clinical purposes in the field of regenerative medicine.”

pluripotent cells that fail to differentiate into a specific tissue are more likely to become cancerous, posing a grave problem for the clinical use of those cells.

Kosai and colleagues studied a gene known as survivin, which is activated in cancer cells. They discovered that survivin is also activated in iPS cells and ES cells that fail to develop into other cells.

The researchers created strains of a genetically modified virus that can proliferate and kill cells that contain survivin. Seven days after the modified virus was introduced into undifferentiated iPS cells and ES cells, all those cells died.

Meanwhile, cells that had turned into the specific tissue survived the introduction of the virus.

The research team will present its findings at a conference of the Japan Society of Gene Therapy on July 25 in Osaka."

That's a big big new, this will accelerate Tsuji, REgience, Keio univ, etc to enter human clinical trials


Other breaktroughts that will help, from only june/july 2015:

-New substrate opens door to mass produced regenerative thérapies

http://www.biopharma-reporter.com/Bi...tive-therapies
http://www.ibtimes.com/are-stem-cell...ssible-2021269

"Researchers at the University of Nottingham in the U.K., have found a synthetic substrate that can be used in the production of billions of stem cells.
Researchers at the University of Nottingham in the U.K. developed the cost-effective substrate that allows the growth of stem cells, and can also survive long-term storage.

"What we are doing here is paving the way for the manufacture of stem cells in large numbers when those therapies are proved to be safe and effective."

"The field of regenerative medicine has snowballed in the last five years and over the coming five years a lot more patients will be receiving stem cell treatments," Denning said. "Clinical trials are still in the very early stages. However, with this kind of product, if we can get it commercialized and validated by the regulators, it could be helping patients in two to three years." And he's from UK, not japan

- MicroRNA Switches Improve Cell Purification
https://beyondthedish.wordpress.com/...-purification/

Stem cell-based therapies usually require the differentiation of stem cells into various cell types that are used for regenerative therapies. Such a strategy requires that the differentiated cells be purified from the rest of the cells. Typically, cell surface proteins are used as the means to distinguish cell types. Unfortunately, many undesired cell types may also share the same cell surface receptors, which will badly compromise the efficiency of cell purification.

Hirohide Saito from the Center for iPS Cell Research and Application (CiRA) at Kyoto University has designed a new way to isolate differentiated cells using microRNAs. This technique appears to be better than using cell surface proteins and it may revolutionize stem cell science.


However, the protocols that are normally used to differentiate iPSCs lead to a mixed population of cells that are very heterogeneous, and the desired cell type has to be isolated from this mixture. Normally, antibodies that bind to surface receptors unique to the desired cell type are used for this purpose but in many cases such purification strategies are inefficient and the cell yield is rather poor. Also, these cell purification techniques have a tendency to damage cells

New RNA-based procedures designed at CiRA may avoid these problems. Hirohide Saito and his colleagues designed tiny RNA molecules (microRNAs or miRNAs) that are designed to detect and sort live cells not by surface receptors, but by miRNAs. MicoRNAs are better markers of cell types and can improve purity levels


Senior Lecturer Yoshinori Yoshida, a heart muscle specialist who works with Professor Saito, immediately saw the potential of this technology.
So Professor Saito and Dr. Yoshida put their heads together to test the effectiveness of miRNA switches

The results were remarkable. Dr. Yoshida saw far better purification than he ever seen with standard methods. Furthermore, because this technology is RNA-based, it does not integrate into the genome and cause mutations. This could potentially make the cells eligible for clinical application.

Just 3 advances from june, if you look the results from one year on Google, it's incredbile there's little breaktroughs every week for cheaper, safely, faster methods.

japanese bioenginereed HFs is coming guys, I definitly believe in a temporallu approval in 2018-2019. Then it will be our choice to take the risk or not ( but it will be low risk with the ipsc tumor-free development)

[champpy]

Excellent information man. Thanks for posting that!

[Renee]

Dr terskikh method using ips cells has cured pattern baldness, Hopefully he begins human trials ASAP. I wonder if the mouses formed any tumors after injections?

[nameless]

Dr terskikh method using ips cells has cured pattern baldness, Hopefully he begins human trials ASAP. I wonder if the mouses formed any tumors after injections?

Dr. Terskikh works for Sanford & Burnham. I call them S & B for short. These guys are a long way off because they do not know how to make the system work for them. S & B is talking about 10 years. What a bunch of idiots.

I think that Shisheido has found a way to do the same thing without stepping on S & B's patent. And Shisheido DOES know how to make the system work for them so they will get it to market sooner. A lot sooner. I think this is what Shisheido is talking about when they say they'll have a cure for baldness by 2018.

I also think SM04554 will be significantly better than any FDA approved medicine already in the marketplace and I anticipate that SM04554 will be in the marketplace in late 2016 or early 2017 at the latest.

[luiza]

Dr terskikh method using ips cells has cured pattern baldness, Hopefully he begins human trials ASAP. I wonder if the mouses formed any tumors after injections?

When Terskikh did that, did all the hair grow back? I didn't research enough about him.

[lacazette]

Here Luiza

http://beaker.sbpdiscovery.org/2015/...grow-new-hair/

“We have developed a method using human pluripotent stem cells to create new cells capable of initiating human hair growth"
“Our stem cell method provides an unlimited source of cells from the patient for transplantation and isn’t limited by the availability of existing hair follicles.”
“In adults, dermal papilla cells cannot be readily amplified outside of the body and they quickly lose their hair-inducing properties,” said Terskikh. “We developed a protocol to drive human pluripotent stem cells to differentiate into dermal papilla cells and confirmed their ability to induce hair growth when transplanted into mice.”
“Our next step is to transplant human dermal papilla cells derived from human pluripotent stem cells back into human subjects,” said Terskikh. “We are currently seeking partnerships to implement this final step.”


He found what jahoda was missing one year before. And yes Renee I think it could really help ,look what the communication manager was saying in june:

"The first trials could start in two years from now if Dr. Terskikh can both fund his research ($1.5 million) and studies into the tumorigenicity of this approach ($5 million).

I hope this gives you more clarity on where this research stands.

Best,"

the price of the studies regarding tumorigenicity means that it is (was?)one of the major problem. So i hope that discovery from japan will give a big booster to test those continuous magnificient mouse studies results INTO HUMAN TRIAL FINALLY !! And if it does, I lower my timeline and I say 2/3 years for the japananese cure lol yes Im on a good mood tonight thanks to Dr Kosai

[lacazette]

Look at some comments in the S&B page lol

"DAVID M MORROW, MD on February 5, 2015 2:52 pm

i am a double board certified dermatologist & cosmetic surgeon with some background and knowledge in stem cells, and in hair transplantation procedures. I would love to be involved in your research. I am currently part of the Cell Surgical Network based in Rancho Mirage & Beverly Hills, which you may have heard of. Please advise.
Dr. Morrow"


"vincent p de villers on August 4, 2015 11:35 am

Dear patrick
I represent the major shareholder in a stem cell company which mass produces ipsc’s.
Im interested i discussing collaboration.
Regards.
Vincent.
Ps im bald but dont care!!!"

"Dr. Alan J. Bauman on January 28, 2015 9:54 am

Dr. Terskikh & Team, Congratulations on your achievement in using stem cells to grow new hair. The entire Bauman Medical Group hair transplant team (and our hair loss patients) wish you much success in the future as you continue this hair loss research which will hopefully produce another viable treatment, or perhaps a cure, for baldness in the near future. Sincerely, Alan J. Bauman M.D. – Diplomate, American Board of Hair Restoration Surgery"

[lacazette]

the communicant don't anwsered to a lot of comments, but he does for this one, i like that

"Thomas on February 4, 2015 10:25 pm

I would donate a lot of money ONLY if the clinical trials were done in Japan as regulations there allow for quick trials as well as a quick commercial release. USA and the FDA is a waste of time. We have one life to live and I am NOT willing to wait any longer for a treatment.

Reply 
Patrick Bartosch on February 13, 2015 2:32 pm

Dear Thomas,

Thank you very much for your comment and your interest in our research. We’ll keep your contact details on file and be in touch once this moves to a stage where we can discuss clinical trials and next steps.

Best,
Patrick Bartosch
Manager, Communications
Sanford-Burnham

[hellouser]

the communicant don't anwsered to a lot of comments, but he does for this one, i like that

"Thomas on February 4, 2015 10:25 pm

I would donate a lot of money ONLY if the clinical trials were done in Japan as regulations there allow for quick trials as well as a quick commercial release. USA and the FDA is a waste of time. We have one life to live and I am NOT willing to wait any longer for a treatment.

Reply 
Patrick Bartosch on February 13, 2015 2:32 pm

Dear Thomas,

Thank you very much for your comment and your interest in our research. We’ll keep your contact details on file and be in touch once this moves to a stage where we can discuss clinical trials and next steps.

Best,
Patrick Bartosch
Manager, Communications
Sanford-Burnham

LOL, that was MY comment!

[Renee]

I believe shiseido will roll out the cure in 2018 via ips cells as they claimed in an article last month. 2 well known ips cell researchers Dr.'s Manabu Ohyama and Jiro Kishimoto work for shiseido. After dr terskikh's success these researchers probably developed their own ipsc method with shiseido backing, hence their claim in that recent article. Btw first hair transplant was done by Japanese researchers in 1939!