STUDY: "this can be used for large-scale preparation of cells to treat hair loss"

[NeedHairASAP]

J Tissue Eng Regen Med. 2015 Jun 29. doi: 10.1002/term.2046. [Epub ahead of print]

Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system.


Dong L1, Hao H1, Liu J1, Tong C1, Ti D1, Chen D1, Chen L1, Li M2, Liu H1, Fu X1, Han W1.
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Abstract
Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study. Wnt-CM was prepared from the supernatant of Wnt1a-expressing bone marrow mesenchymal stem cells (BM-MSCs) that maintain the hair-inducing gene expression of DP cells. The collagen-chitosan scaffold cells (CCS cells) were constructed using a two-step method by inoculating the Wnt-CM-treated DP cells and epidermal (EP) cells into the CCS. The cells in the air-liquid culture formed dermal condensates and a proliferative cell layer in vitro. The CCS cells were able to induce hairregeneration in nude mice. The results demonstrate that Wnt-CM can maintain the hair induction ability of DP cells in expansion cultures, and this approach can be used for large-scale preparation of CCS cells in vitro to treat hair loss.



Copyright © 2015 John Wiley & Sons, Ltd.


KEYWORDS:
Wingless-type mouse mammary tumour virus integration site; bone marrow mesenchymal stem cell; conditioned medium; dermal papilla; hairfollicles; regeneration; three-dimensional; tissue engineering