Hair regrowth through wound healing process after ablative fractional laser treatment

[hair4us2]

SM is five years away. These lasers treatments are available all over the US and the world RIGHT NOW (albeit they use it on peoples faces and not their scalps)

So why doesn't someone try the lasers on their hair to see if this causes regrowth in humans as opposed to mouse?

[NeedHairASAP]

Induction of De Novo Hair Regeneration in Scars After Fractionated Carbon Dioxide Laser Therapy in Three Patients

THOMAS M. BEACHKOFSKY MD1, J. SCOTT HENNING DO2 andCHAD M. HIVNOR MD2
Article first published online: 14 APR 2011

DOI: 10.1111/j.1524-4725.2011.01934.x

© 2011 by the American Society for Dermatologic Surgery, Inc.

[NeedHairASAP]

http://laserinmedici...n disorder.pdf

Results
Fractional ErYAG laser treatment was well tolerated, with slight stinging
phenomena during laser procedure. In all patients, the initial reactions to treatment
consisted of erythema and minimal swelling in the treated areas; the patients reported a
burning sensation but no significant pain. The erythema lasted between 2 and 10 days
(mean, 4.6 days), and its severity was correlated with the initial erythema level.
In all patients sclerosis regressed greatly, skin score markedly decreased and
dermoscopy findings objectively showed a reduction of amount of cicatrisation, skin
hardness and dischromia. At the end of the follow up period number of hair follicule in
the treated zone increased greatly.




The role of vascular endothelial growth factor in fractional laser resurfacing with the carbon dioxide laser

Abstract
The aim of this study was to analyze the role of vascular endothelial growth factor (VEGF) in mechanisms of cutaneous remodeling induced by fractional CO2 laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO2 laser treatment. Biopsies were taken 1 h, and 1, 3, 7, 14, 28 and 56 days after treatment. Skin samples VEGF expression was evaluated by immunohistochemistry and ELISA, fibroblasts by hematoxylin-eosin staining, and types I and III collagen by ELISA. Staining for VEGF was found in many types of cell including fibroblasts. The amount of VEGF in the skin of laser-treated areas had increased significantly compared to that in the control areas on days 1 and 3 (P?<?0.05, P?<?0.01, respectively), then decreased by day 7 after treatment and returned to the baseline level. The number of fibroblasts in the skin of the laser-treated areas had increased significantly compared to that in control areas on days 3, 7, 14, 28 and 56 after irradiation (P?<?0.05, P?<?0.01, P?<?0.01, P?<?0.01, P?<?0.01, respectively). The amount of type I collagen was significantly higher in the skin of the laser-treated areas compared to that in control areas from day 28 to day 56 (P?<?0.05, respectively), and type III collagen was significantly higher from day 3 to day 56 (P?<?0.05, P?<?0.05, P?<?0.05, P?<?0.05, P?<?0.01, respectively). There was a positive correlation between the level of VEGF and fibroblast proliferation early stage after laser treatment (r?=?0.853, P?<?0.01), but there was no correlation after the first week (r?=??0.124, P?>?0.05). The amounts of type I and III collagen showed no significant correlations with the expression of VEGF in the late stages after laser treatment (r?=?0.417, P?>?0.05 and r?=?0.340, P?>?0.05, respectively). The results suggest that VEGF might be mainly involved in the early stages of wound healing, including the stages of acute inflammation, fibroblast proliferation and vessel formation induced by fractional CO2 laser resurfacing





Clinical effects of non-ablative and ablative fractional lasers on various hair disorders: a case series of 17 patients

Abstract

Background and objectives: Both ablative and non-ablative fractional lasers have been applied to various uncommon hair disorders. The purpose of this study was to demonstrate the clinical effects of fractional laser therapy on the course of primary follicular and perifollicular pathologies and subsequent hair regrowth. Materials and methods: A retrospective review of 17 patients with uncommon hair disorders - including ophiasis, autosomal recessive woolly hair/hypotrichosis, various secondary cicatricial alopecias, pubic hypotrichosis, frontal fibrosing alopecia, and perifolliculitis abscedens et suffodiens - was conducted. All patients had been treated with non-ablative and/or ablative fractional laser therapies. Results: The mean clinical improvement score in these 17 patients was 2.2, while the mean patient satisfaction score was 2.5. Of the 17 subjects, 12 (70.6%) demonstrated a clinical response to non-ablative and/or ablative fractional laser treatments, including individuals with ophiasis, autosomal recessive woolly hair/hypotrichosis, secondary cicatricial alopecia (scleroderma and pressure-induced alopecia), frontal fibrosing alopecia, and perifolliculitis abscedens et suffodiens. Conversely, patients with long-standing ophiasis, surgical scar-induced secondary cicatricial alopecia, and pubic hypotrichosis did not respond to fractional laser therapy. Conclusion: Our findings demonstrate that the use of non-ablative and/or ablative fractional lasers promoted hair growth in certain cases of uncommon hair disorders without any remarkable side effects.



Read More: http://informahealth...172.2013.764436



Up-regulation of fibroblast growth factor (FGF) 9 expression and FGF-WNT/?-catenin signaling in laser-induced wound healing

Abstract
Fibroblast growth factor (FGF) 9 is secreted by both mesothelial and epithelial cells, and plays important roles in organ development and wound healing via WNT/?-catenin signaling. The aim of this study was to evaluate FGF9 expression and FGF-WNT/?-catenin signaling during wound healing of the skin. We investigated FGF9 expression and FGF-WNT/?-catenin signaling after laser ablation of mouse skin and adult human skin, as well as in cultured normal human epidermal keratinocytes (NHEKs) upon stimulation with recombinant human (rh) FGF9 and rh-transforming growth factor (TGF)-?1. Our results showed that laser ablation of both mouse skin and human skin leads to marked overexpression of FGF9 and FGF9 mRNA. Control NHEKs constitutively expressed FGF9, WNT7b, WNT2, and ?-catenin, but did not show Snail or FGF receptor (FGFR) 2 expression. We also found that FGFR2 was significantly induced in NHEKs by rhFGF9 stimulation, and observed that FGFR2 expression was slightly up-regulated on particular days during the wound healing process after ablative laser therapy. Both WNT7b and WNT2 showed up-regulated protein expression during the laser-induced wound healing process in mouse skin; moreover, we discerned that the stimulatory effect of rhFGF9 and rhTGF-?1 activates WNT/?-catenin signaling via WNT7b in cultured NHEKs. Our data indicated that rhFGF9 and/or rhTGF-?1 up-regulate FGFR2, WNT7b, and ?-catenin, but not FGF9 and Snail; pretreatment with rh dickkopf-1 significantly inhibited the up-regulation of FGFR2, WNT7b, and ?-catenin. Our results suggested that FGF9 and FGF-WNT/?-catenin signaling may play important roles in ablative laser-induced wound healing processes.



Fractional Photothermolysis Laser Treatment of Male Pattern Hair Loss


BACKGROUND Various trials have been conducted on the management of male pattern hair loss (MPHL). A variety of laser and light sources have been used for the treatment of MPHL.

OBJECTIVE To understand the effects of a 1,550-nm fractional erbium-glass laser on the hair cycle in an alopecia mouse model and to study the clinical effects of the same laser used as treatment for MPHL.

MATERIALS AND METHODS Irradiation was applied to the shaved skin of C3H/HeN mice using various energy and density settings and varied irradiation intervals. In a clinical pilot study involving human subjects, 20 participants were treated over five sessions at 2-week intervals. A fractional photothermolysis laser was used at the energy of 5 mJ and a total density of 300 spots/cm2.

RESULTS In the animal study, the hair stimulation effects were dependent upon the energy level, density, and irradiation interval. The anagen conversion of hair and the increase in Wnt 5a, ?-catenin signals were observed. In the human pilot study, incremental improvements in hair density and growth rate were observed.

CONCLUSIONS This pilot study showed that a 1,550-nm fractional erbium-glass laser might induce hair growth, but more intensive studies are required to clarify the clinical applications of this treatment.

The authors have indicated no significant interest with commercial supporters.





Induction of De Novo Hair Regeneration in Scars After Fractionated Carbon Dioxide Laser Therapy in Three Patients

THOMAS M. BEACHKOFSKY MD1, J. SCOTT HENNING DO2 andCHAD M. HIVNOR MD2
Article first published online: 14 APR 2011

DOI: 10.1111/j.1524-4725.2011.01934.x

© 2011 by the American Society for Dermatologic Surgery, Inc.

[nameless]

SM is five years away. These lasers treatments are available all over the US and the world RIGHT NOW (albeit they use it on peoples faces and not their scalps)

Needhairasap I appreciate your efforts but I think that there are two issues:

1. They haven't gotten the laser method of hair regrowth figured out completely. All the T's are not crossed and all the I's are not dotted.

2. Just because the FDA let's you use lasers on the back doesn't mean the FDA will let you use lasers on the head.

[BrianH123]

I made the mistake of getting laser hair removal on my back and after wasting ~3000$ , i came to the conclusion it didn't work and there was more hair on my back when i had started. That was 14 treatments to be exact. I would joke around with the girls who worked there saying they should use it on my head because it doesn't do a dam thing to take hair off, just more grows. I'm aware the properties of hair on my back are not the same as on the top of my head, so i don't know if it would work the same. but it makes me feel like this could be a possible way to help hair grow back on my head seeing how the before and after of my back hair laser treatments went.

[NeedHairASAP]

Needhairasap I appreciate your efforts but I think that there are two issues:

1. They haven't gotten the laser method of hair regrowth figured out completely. All the T's are not crossed and all the I's are not dotted.

2. Just because the FDA let's you use lasers on the back doesn't mean the FDA will let you use lasers on the head.

1. This statement is empty. The only issues are (a) which laser is best, there are maybe 3-5 potential types that could be used and (b) which settings are best, there are maybe 3-4 setting to figure out..... and I'm not really sure what you're implying... we haven't figured these two (relatively simple) aspects, therefore.....? we should not talk about this? Therefore we should not pursue this? I just don't even understand what your point is in your #1 point.... if you're just stating that they haven't figured this out exactly... just to state the obvious, then okay... thanks.

2. What does the FDA have to do with this? If it works, I'll fly to mexico (or fill in the blank with 50 other countries) and then I'll have this done. I could really care less about the FDA in this case....and I think we probably all should not care. I'm not anti-FDA but again, I don't really understand why you said this.... The FDA hasn't approved this yet.. therefore? Are you implying we should just go back to talking about hypothetical topicals that may be available in five years? Or maybe we can hypothesize and argue about if AAPE will give us 100 extra hairs or 125 extra hairs... I mean what is the point of saying #2? You need to put more "therefore"s in your posts... e.g. "XYZ is a fact, THEREFORE [insert action/conclusion]"


anyway... EVERYONE! Laser wounding is the only thing that will be available in the next 3-4 years baring a legitimate god-given miracle that comes out of nowhere.

On top of that, there is potential that this can trigger regeneration of every single hair on your head... where as all these other things (bim, HTs, minox, fin, RU, etc. etc.) are things that MAY regrow 10-20% of your hair... laser wounding-to-regeneration is the best bet we have.

This is based on facts.. we know dermarolling works... we have seen campfire man.... we know the recent plucking studies.... etc. etc. etc.... the question is... how do we "do a campfire man" in a CONTROLLED, SAFE, CONSISTENT application?


Answer: LASERS

[NeedHairASAP]

I made the mistake of getting laser hair removal on my back and after wasting ~3000$ , i came to the conclusion it didn't work and there was more hair on my back when i had started. That was 14 treatments to be exact. I would joke around with the girls who worked there saying they should use it on my head because it doesn't do a dam thing to take hair off, just more grows. I'm aware the properties of hair on my back are not the same as on the top of my head, so i don't know if it would work the same. but it makes me feel like this could be a possible way to help hair grow back on my head seeing how the before and after of my back hair laser treatments went.

same here... there are many reports online of laser hair removal ending up in more hair.



That said, I was wrong... these lasers are not used for laser hair removal. They are used for face stuff... essentially they zap the top layer of skin off of your face, and the skin rejuvenates more youthful.... see Kardashians et al.

[nameless]

I made the mistake of getting laser hair removal on my back and after wasting ~3000$ , i came to the conclusion it didn't work and there was more hair on my back when i had started. That was 14 treatments to be exact. I would joke around with the girls who worked there saying they should use it on my head because it doesn't do a dam thing to take hair off, just more grows. I'm aware the properties of hair on my back are not the same as on the top of my head, so i don't know if it would work the same. but it makes me feel like this could be a possible way to help hair grow back on my head seeing how the before and after of my back hair laser treatments went.

Can you please post before and after pics of the hair growth on your back?

[nameless]

Needhairasap, has science completely figured out ALL of the details to regrow human hair with lasers or do they still have to figure out some more details? Please show me before and after pics demonstrating hair regowth ON HUMANS using lasers. It would be a lot easier to take this serious if you had positive regrowh pics in humans. Mice don't mean much.

[hair4us2]

Needhairasap I appreciate your efforts but I think that there are two issues:

1. They haven't gotten the laser method of hair regrowth figured out completely. All the T's are not crossed and all the I's are not dotted.

2. Just because the FDA let's you use lasers on the back doesn't mean the FDA will let you use lasers on the head.

What has the FDA got to do with allowing people to use lasers on their scalp?????

The FDA is not the World's Police Health authority! There is much more to the world than the frickin FDA!!

If I want to use lasers on my head, or my nose or my bum cheeks then I don't need the approval of the FDA.

[NeedHairASAP]

Needhairasap, has science completely figured out ALL of the details to regrow human hair with lasers or do they still have to figure out some more details? Please show me before and after pics demonstrating hair regowth ON HUMANS using lasers. It would be a lot easier to take this serious if you had positive regrowh pics in humans. Mice don't mean much.

Obviously if before and after pictures existed I wouldn't be here trying to convince people to look further into this...

The point is: these lasers are at almost every cosmetic clinic in the world. It's a matter of figuring out the type and the settings....

we already "know" that it should work if the right laser and settings are used.

lastly, i dont care if you "take this serious"-- because either way, it's serious.

[joachim]

i have to agree that the whole wounding approach seems very attractive, effective, and when the last steps are figured out, also relatively easy. it's really only about figuring out the right wavelength, intensity, and duration of the laser burning on the scalp. some of those studies already give some good hints into the right direction.
also, since dr. cots from follica came up with his wounding theories, it got even more obvious that wounding is a nice approach. they even stated they are able to consistently produce new hair de novo but for some reason dr. cots says they still don't have a cure, after all their laser patenting stuff. i always had the feeling they are holding something back.

but all those storyies about campfire guy, or more hair growing after hair removal. it all points into the wounding phenomenon. actually it's not that easy to understand. skin cells are beeing burned off and the body starts a repair mechanism. chemical signals are released at the wounding spot, and the cells are multiplying themselves to repair the damaged spot. during multiplication those cells decide to whether become simple skin cells or hair cells (DP cells or whatever). dr. cots mentioned there is a short time window where this decision is made by the cells.
of course, controlling this cells consistently is the difficulty.

but if a laser hair removal can lead to lots of denovo hairs already, then it can't be that hard to figure out.
our best chance would be to exactly try it with that same laser from the hair removal. if we could have a chance go get our hands on it.
once figured out, nobody needs the fda. the treatment plan would go viral and everybody would find access to such a laser one way or another.

[joachim]

needhairasap: do you have the possibilities to drive this topic forward? seems you're a good research guy with the right knowledge. we need to find access to such a laser anyway.

[BrianH123]

Unfortunately i didn't take before pictures .. So i could take an after picture, i'm not really sure if that would help tho because there is nothing to compare it to. I also have gone back to waxing my back once a month so it would take 2-3 months to get a real idea of how dense and dark the new hair is. My shoulders have black dark hair now when there used to be nothing, but again i have no photos of before so i'm kinda useless. Id love to see if this could work tho!

[joachim]

Unfortunately i didn't take before pictures .. So i could take an after picture, i'm not really sure if that would help tho because there is nothing to compare it to. I also have gone back to waxing my back once a month so it would take 2-3 months to get a real idea of how dense and dark the new hair is. My shoulders have black dark hair now when there used to be nothing, but again i have no photos of before so i'm kinda useless. Id love to see if this could work tho!

hey brian, do you think there is a chance the place where you did the laser hair removal could try out the laser on your scalp, on a small spot only (5x5mm or so)? would you even give it a shot if they would let you do the experiment? as you had great "success" with growing new hairs on the back, why not try exactly the same treatment on the scalp? what if it really works? you would have found the cure by accident.

at least we should know the product name of the laser (manufacturer and exact type of the device). if it's a device with configurable laser intensity, maybe the could give the exact setting, so that we could do a bit more research on this used setting.