Anyone have any news from Lauster/Jahoda teams?

[liba]

Their methods hold the key to the cure imo, but it is a shame that they are silent for so long and didn't make any progress on it, or at least didn't reveal any information from what I know... Seems that Lauster just created some brand new hair follicle and don't give a crap anymore, truly an outrage.

[hellouser]

Their methods hold the key to the cure imo, but it is a shame that they are silent for so long and didn't make any progress on it, or at least didn't reveal any information from what I know... Seems that Lauster just created some brand new hair follicle and don't give a crap anymore, truly an outrage.

Dr. Lauster basically found the cure 5 years ago. If you think nothing has been done since, you have to assume they either gave up (unlikely) or hit a road block (also unlikely). However, 5 years ago they were already able to create follicles albeit small ones.

They've got the cure.... thats not even a question except that the university is holding the patent. You want the cure? You're going to have to make a case that you're worthy of living a better life, because even if this got released, you'd still get screwed because this is supposed to be for testing cosmetics instead of animal testing. So yeah... bald men are continuously getting snubbed.

[FooFighter]

Dr. Lauster basically found the cure 5 years ago. If you think nothing has been done since, you have to assume they either gave up (unlikely) or hit a road block (also unlikely). However, 5 years ago they were already able to create follicles albeit small ones.

They've got the cure.... thats not even a question except that the university is holding the patent. You want the cure? You're going to have to make a case that you're worthy of living a better life, because even if this got released, you'd still get screwed because this is supposed to be for testing cosmetics instead of animal testing. So yeah... bald men are continuously getting snubbed.

That follicle that they created you cant use it for cosmetic surgery, because that follicle is tiny and dont have gene expressions.

[hellouser]

That follicle that they created you cant use it for cosmetic surgery, because that follicle is tiny and dont have gene expressions.

That was 5 years ago.

[hellouser]

That follicle that they created you cant use it for cosmetic surgery, because that follicle is tiny and dont have gene expressions.

That was 5 years ago. You can be pretty sure things have changed since.

[mikes23]

I think they solved the gene expression, I believe the problem is, being able to implant it and having it survive. I know dr gardner talked about needing the right environment for the follicle to survive. The world vongress for hair meeting is in November this year, in Miami. so we will be able to see how dr lauster is progressing. Also wake forest university are working on lab grown follicles too. I just wish Desmond could get in touch with Dr Lauster to see how they are doing, instead of us waiting a whole year to find out.

[liba]

Dr. Lauster basically found the cure 5 years ago. If you think nothing has been done since, you have to assume they either gave up (unlikely) or hit a road block (also unlikely). However, 5 years ago they were already able to create follicles albeit small ones.

They've got the cure.... thats not even a question except that the university is holding the patent. You want the cure? You're going to have to make a case that you're worthy of living a better life, because even if this got released, you'd still get screwed because this is supposed to be for testing cosmetics instead of animal testing. So yeah... bald men are continuously getting snubbed.

Damn I hope we can do something to get them do the things they ought to do... At least remind them how great it can be if they put their method in to hair loss treatment..

[liba]

I think they solved the gene expression, I believe the problem is, being able to implant it and having it survive. I know dr gardner talked about needing the right environment for the follicle to survive. The world vongress for hair meeting is in November this year, in Miami. so we will be able to see how dr lauster is progressing. Also wake forest university are working on lab grown follicles too. I just wish Desmond could get in touch with Dr Lauster to see how they are doing, instead of us waiting a whole year to find out.

Yeah that's kinda good news at least... Have they decided to come to the meeting?

[karxxx]

A Johoda ' team ?
Not to clone another team ?

[noisette]

A multi-organ chip co-culture of neurospheres and liver equivalents for long-term sub

Hello guys, I have a recent paper (2/2015) from Lauster. This can help ?

A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing

Journal of Biotechnology (Impact Factor: 2.88). 02/2015; DOI: 10.1016/j.jbiotec.2015.02.002
Source: PubMed

ABSTRACT Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, often do not accurately predict drug toxicity, leading to high attrition rates in clinical studies (Paul et al., 2010). The phylogenetic distance between humans and laboratory animals is enormous, this affects the transferability of animal data on the efficacy of neuroprotective drugs. Therefore, many neuroprotective treatments that have shown promise in animals have not been successful when transferred to humans (Dragunow, 2008; Gibbons and Dragunow, 2010). We present a multi-organ chip capable of maintaining 3D tissues derived from various cell sources in a combined media circuit which bridges the gap in systemic and human tests. A steady state co-culture of human artificial liver microtissues and human neurospheres exposed to fluid flow over two weeks in the multi-organ chip has successfully proven its long-term performance. Daily lactate dehydrogenase activity measurements of the medium and immunofluorescence end-point staining proved the viability of the tissues and the maintenance of differentiated cellular phenotypes. Moreover, the lactate production and glucose consumption values of the tissues cultured indicated that a stable steady-state was achieved after 6 days of co-cultivation. The neurospheres remained differentiated neurons over the two-week cultivation in the multi-organ chip, proven by qPCR and immunofluorescence of the neuronal markers βIII-tubulin and microtubule-associated protein-2. Additionally, a two-week toxicity assay with a repeated substance exposure to the neurotoxic 2,5-hexanedione in two different concentrations induced high apoptosis within the neurospheres and liver microtissues, as shown by a strong increase of lactate dehydrogenase activity in the medium. The principal finding of the exposure of the co-culture to 2,5-hexanedione was that not only toxicity profiles of two different doses could be discriminated, but also that the co-cultures were more sensitive to the substance compared to respective single-tissue cultures in the multi-organ-chip. Thus, we provide here a new in vitro tool which might be utilized to predict the safety and efficacy of substances in clinical studies more accurately in the future.
Copyright © 2015. Published by Elsevier B.V.

[liba]

Hello guys, I have a recent paper (2/2015) from Lauster. This can help ?

A multi-organ chip co-culture of neurospheres and liver equivalents for long-term substance testing

Journal of Biotechnology (Impact Factor: 2.88). 02/2015; DOI: 10.1016/j.jbiotec.2015.02.002
Source: PubMed

ABSTRACT Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, often do not accurately predict drug toxicity, leading to high attrition rates in clinical studies (Paul et al., 2010). The phylogenetic distance between humans and laboratory animals is enormous, this affects the transferability of animal data on the efficacy of neuroprotective drugs. Therefore, many neuroprotective treatments that have shown promise in animals have not been successful when transferred to humans (Dragunow, 2008; Gibbons and Dragunow, 2010). We present a multi-organ chip capable of maintaining 3D tissues derived from various cell sources in a combined media circuit which bridges the gap in systemic and human tests. A steady state co-culture of human artificial liver microtissues and human neurospheres exposed to fluid flow over two weeks in the multi-organ chip has successfully proven its long-term performance. Daily lactate dehydrogenase activity measurements of the medium and immunofluorescence end-point staining proved the viability of the tissues and the maintenance of differentiated cellular phenotypes. Moreover, the lactate production and glucose consumption values of the tissues cultured indicated that a stable steady-state was achieved after 6 days of co-cultivation. The neurospheres remained differentiated neurons over the two-week cultivation in the multi-organ chip, proven by qPCR and immunofluorescence of the neuronal markers βIII-tubulin and microtubule-associated protein-2. Additionally, a two-week toxicity assay with a repeated substance exposure to the neurotoxic 2,5-hexanedione in two different concentrations induced high apoptosis within the neurospheres and liver microtissues, as shown by a strong increase of lactate dehydrogenase activity in the medium. The principal finding of the exposure of the co-culture to 2,5-hexanedione was that not only toxicity profiles of two different doses could be discriminated, but also that the co-cultures were more sensitive to the substance compared to respective single-tissue cultures in the multi-organ-chip. Thus, we provide here a new in vitro tool which might be utilized to predict the safety and efficacy of substances in clinical studies more accurately in the future.
Copyright © 2015. Published by Elsevier B.V.

Hmm.. i don't know exactly what to say... It is good that they are still active on something, but it is disappointing that the newest finding they are announcing is not a breakthrough of hair follicle issue they were previous onto, but something irrelevant.

[Desmond84]

The Chinese have mastered Jahoda's technique and are rapidly developing methods to take it to clinical trials. Here's the paper they published last month:

http://pubs.acs.org/doi/abs/10.1021/acsami.6b00202

Interestingly enough, DP cells maintained their Trichogenicity in passage 8 which is unheard of. Hair sprouted after expanding these cells 8 times. Let's see what more this year brings.

Important note: the DP cells used were human. They were just injected into mice! In before, another mouse study

[Arashi]

The Chinese have mastered Jahoda's technique and are rapidly developing methods to take it to clinical trials. Here's the paper they published last month:

http://pubs.acs.org/doi/abs/10.1021/acsami.6b00202

Interestingly enough, DP cells maintained their Trichogenicity in passage 8 which is unheard of. Hair sprouted after expanding these cells 8 times. Let's see what more this year brings.

Important note: the DP cells used were human. They were just injected into mice! In before, another mouse study

8 passages, that's an accomplishment in itself indeed, nice to see this progress. However, key problem with Jahoda's achievements were the lack of correct cosmetic attributes like hair color and thickness. Does this article reveal anything here ?

[allTheGoodNamesAreTaken]

The Chinese have mastered Jahoda's technique and are rapidly developing methods to take it to clinical trials. Here's the paper they published last month:

http://pubs.acs.org/doi/abs/10.1021/acsami.6b00202

Interestingly enough, DP cells maintained their Trichogenicity in passage 8 which is unheard of. Hair sprouted after expanding these cells 8 times. Let's see what more this year brings.

Important note: the DP cells used were human. They were just injected into mice! In before, another mouse study

So how many 'passages' does it take before you have a working product?

[Arashi]

So how many 'passages' does it take before you have a working product?

passages just means dividing cells. So you take a cell, make 2 out of them, now you have 2 cells after on passage. Then you this again and you have 4 cells after 2 passages. So 8 passages just means they managed to make 256 dp cells out of just 1 dp cell and all those cells still were able to induce a hair follicle.