Dr Nigam, my own experience

[One]

Also, Dr Nigams, you were talking earlier about a max of 5000 grafts/session. But if approx 1000 grafts already take 3 days, does that mean that 5000 grafts equals 15 office days = 3 weeks ?!?!

That's right, Dr Nigam I do not know if updates on the forum writes her or his secretary but often do not understand what it means.

Read a bunch of names (cells dp-prp, re-growth factors), numbers, bulbs cut, put some in the culture, other implanted confuses us a lot.


Moreover, often does not respond in a direct manner to the questions.

Mine is the following: Why do not you have innestat all grafts at once? Are you able to engage with a density of 40-50-60 cm2 grafts in a session?

[drnigams]

One,
Answer to your specific question if i understood correctly...

Do you want to know..why i give local anesthetic and extract approx 200 grafts from donor when patient lies in prone position and turn the patient into supine position give local anesthesia and implant at the recipient....

This is because,i want to expose the grafts to minimum time, outside the scalp...as against extract all grafts and than implant after bisection..
This also consumes time...
Remember invitro doubling is not fue..there are multiple steps and 9/10 personnel team working together and not 2/3 as in fue..
With invitro ,i can implant 1500..to 2000G per day...provided all medical parameters are normal..

That's right, Dr Nigam I do not know if updates on the forum writes her or his secretary but often do not understand what it means.

Read a bunch of names (cells dp-prp, re-growth factors), numbers, bulbs cut, put some in the culture, other implanted confuses us a lot.


Moreover, often does not respond in a direct manner to the questions.

Mine is the following: Why do not you have innestat all grafts at once? Are you able to engage with a density of 40-50-60 cm2 grafts in a session?

[Arashi]

I guess my point is: if you extracted 1119 grafts, sent 175 grafts for isolating and sourcing progenitor stem cells (which you state couldnt be used therefore in donor/recipient) and the 75 for DP culture can't be used either ... that means you had only 869 grafts for donor/recipient, yet you implanted 1044 into recipient ... does that mean you implanted way less in donor !?! It's a bit confusing.

[One]

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[drnigams]

Invitro hairdoubling...equals 1500 doublings per day..which means ..in 4 days..approx.6000grafts doublings ..per session..repeat the same after 6 to 12 weeksr visit ..

Also, Dr Nigams, you were talking earlier about a max of 5000 grafts/session. But if approx 1000 grafts already take 3 days, does that mean that 5000 grafts equals 15 office days = 3 weeks ?!?!

[didi]

wow, nice dr nigam.

12 000 grafts in 2-3 months, no scarring/white dots+90% growth and donor regeneration?

[TO YOUNG TO RETIRE]

what is the waiting time Dr? i mean when is the next available free to book time?

Invitro hairdoubling...equals 1500 doublings per day..which means ..in 4 days..approx.6000grafts doublings ..per session..repeat the same after 6 to 12 weeksr visit ..

[drnigams]

I hope this will be clear now...

1)Total1475 extraction drills at the donor.

2)Out of these1475 drills.175grafts extracted for stemcells and 75 grafts extracted for dp culture and sent to lab.

3)106grafts extracted for dermal papilla cell isolation and than implanted back at the donor from where they were extracted without dermal papilla,these were not bisected.

4)1119 grafts left were bisected and double the number i.e 2238 grafts created by bisection.

5)1044 implanted at the recipient and 1194buiisected grafts were implanted at the donor...why we implant little more at the donor is to fill all extraction drills..

So ,if we get 1475-175-75=1225x2=2450-106(as they were not doubled,used for dpcell isolation)= 2344 grafts at donor and recipient combine is what we should get, for 100%regen in total at donor and recipient...anything less could be due to telogen/exogen(10%to15%),if at all you want to count them...anything less than this, means corresponding regeneration percent ...
I can understand..invitro is new..and the process is different from fut/fue/invivo doubling..hence please feel free to clarify...

I don't understand the numbers. How should we see them ? Total extracted grafts was 1119. 175 sent out for isolation, were these whole grafts (so before split ?). Same question for the 75 grafts for DP culture. Then the 106 grafts for DP isolation, were they split ?

You say 1044 halves were planted into recipient. How many went into donor ? And I don't understand this sentence "Most of the lower half of the grafts part hair bulb was implanted as the donor except approx 200 lower half injected most of the upper half of the follicle implanted approx 75 grafts were drilled but grafts not extracted from these 75 drills at donor"

Thanks

[Arashi]

I hope this will be clear now...

1)Total1475 extraction drills at the donor.

2)Out of these1475 drills.175grafts extracted for stemcells and 75 grafts extracted for dp culture and sent to lab.

3)106grafts extracted for dermal papilla cell isolation and than implanted back at the donor from where they were extracted without dermal papilla,these were not bisected.

4)1119 grafts left were bisected and double the number i.e 2238 grafts created by bisection.

5)1044 implanted at the recipient and 1194buiisected grafts were implanted at the donor...why we implant little more at the donor is to fill all extraction drills..

So ,if we get 1475-175-75=1225x2=2450-106(as they were not doubled,used for dpcell isolation)= 2344 grafts at donor and recipient combine is what we should get, for 100%regen in total at donor and recipient...anything less could be due to telogen/exogen(10%to15%),if at all you want to count them...anything less than this, means corresponding regeneration percent ...
I can understand..invitro is new..and the process is different from fut/fue/invivo doubling..hence please feel free to clarify...

I'm not sure I understand. When you say did 1475 extraction drills, does this mean that you extracted 1475 grafts ? Cause if that would be the case then if you'd want to attain 100% regrowth, you'd need to see 2950 grafts regenerate and not 2344 ?

[One]

Dr Nigam, even I don't understand.

Until now you said that with your technique in vitro you can choose to put half of the hair in the donor, and then have regeneration, or NOT put them both in the graft and recipient.

Now you said: "In two sittings we will extract 5000 and 6400 grafts, with one child of 4/5 days on an avg."

But if we extract 5000 or 6400 grafts and we want to regeneration in donor, we will have only half the recipient, that is 2500 + 3200 = 5700 or am I wrong?