WCHR 2013 Topic Follicle neogenesis by DP Cells @Dr. Nigam's

[drnigams]

Kindly find below the abstract which was discussed at WCHR 2013 Edinburgh which I visited recently and had a interaction with Collin Jahoda on the same. Scientific minded members can discuss it further for hair follicle neogenesis

P220

Human hair follicle neogenesis using microvironmentally reprogrammed dermal papilla cells


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CA Higgins, CA Jahods2 and AM christiano 1.3 Department of Dermatology, Columbia University, new York, USA , 2 Biological and Biomedical sciences, Durham University, Durham UK and 3 Genetics and Development, Columbia University, New York,USA

Hair Follicle (HF) neogensis refers to the generation of an entirely new HF in recipient skin using HF dermal papilla (DP) cells. This has been extensively demonstrated in rodent skin , either using intact DP or using intact Dp or using caltured DP cells. In contrast , HF neogensis in human skin has not previously been achieved using human cells. We performed global transcriptional profiling of both intact and cultured Dp cells using Affymetrix U133 Plus 2.0 array , which revealed several pathways expressed in intact5 DP , which are capable of neogenesis , but absent in caltured cells, that lack the micro environmental and anatomical context of intact DP is to grow the cells in hanging drops, which results in the formation of DP spheroids. We then profiled DP spheroids for changes in gene expression and determined that the average correlation coefficient between the transcriptomes of intact DP and the cultured cells is 0.42 , whereas that between the intact DP 3D culture. To evaluate whether recapitulation of the DP signature equated to a restored inductive potential, we established a contextual human – to – human HF neogenesis assay that could be used to assess the inductive capacity of human DP cells in human skin. When we micro implanted DP spheroids into recombined foreskins placed onto the back of SCID mice, we observed marked HF neogensis by 6 weeks, showing for the first time that intact human DP can induce de novo human HFs. We conclude that the partial restoration of the transcriptional profile in human Dp cells, achieved simply by growing the cells in a 3d spherical microenvironment, is sufficient in some instances to restore the inductive capacity of Dp cell cultures and elicit human HF neogenesis.P220