50 grafts patch test in Vitro Hair Doubling as requested by GC @Dr. Nigam's

[UK Boy]

DIDI,I was there in turkey few months back,
I met few doctors and they will mail me regarding the regulations ,they had no idea what i wwas talking they were traditional fut/fue surgeons.
DIDI ,GC,arashiu,IM,
your comments required
on the patch test i am performing the day after
NAME of the procedure-STEMCELLS-PLUCK & IMPLANT ....the new MPB solution.
USP of the procedure--100% guarantee of donor regen,no punch,no needle use,no mark, donor,repeat use of donor in 1month,most cost effective.Conversion of nw7 to nw1/2 in 2 sessions i month apart each session of 3 to 4 days.
As you see in the picture before this is the picture of the same guy of 15 G patch test .

As you can see the safe donor area of most of the patient is spread between a length of 40 to 45 cm an average width of 8 cm.

That means we have total safe donor area of 320 to 360 sq cm.

Density of various people may range from 30 to 90 graft per sq cm at the safe donor area.

For Donor Density of 30 graft per sq cm patient we have access to 9600 graft and
For 40 graft per sq cm we have 12800
For 50 graft per sq cm 16000

Now since we are not even giving a pin prick at the donor but plucking the hair like eye brow (but yes in a special way with special equipment so that I should have at least 3 mm part of hair follicle from the skin surface under ultra sound guidance.

Even if we get 50% regeneration (Although i expect at least 70% regen at the recipient after refinement of technique) with addition of activated stem cells, DP Cells, DP, ECM, PRP, Growth factors. The plucked follicle will have bulge area of stem cells which are rich in CD 34 + stem cells and they can be considered as partial hair. The DP part which is missing in the plucked follicle will be compensated by DP cells and DP injection from the few body hair.

Like eye brow plucking where the donor cannot be damage I can easily pluck 4000 grafts per day and can implant at the recipient. Hence in 3 days we can have 12000 grafts. NW7 to NW2 lesser NW will require lesser time. With 50% regeneration the procedure can repeated after one month, the time when the donor grafts is re-visible again for plucking.



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/01.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/02.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/03.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/04.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/05.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/06.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/07.JPG



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/M...t/Large/08.JPG

stemcells

Wow Dr. Nigam, if you master 'Plucking' you really will have the cure for baldness. Have you studied all of Dr.Cooley's work regarding plucking? There was a lot of excitment about this procedure back in 2011, a number of long standing hair transplant doctors in the US did plucking trials in their clinics and the outcome was that the recipient yield was no good. Do you really think the DP injections will be the key to the recipient survival? How long will we have to wait to truely evaluate the recipient? You're giving so much hope to so many people Dr. Nigam, I once suffered keloid scaring and am therefore terrified to ever try a normal hair transplant in case I got keloid scaring but with doctors such as yourself, Dr. Gho and Dr. Wesley working on ways to perform hair transplants with no risk of scaring I'm starting to get hope that the future will offer me several options if I decided to have a hair transplant. Obviously yours is most exciting as it offers greatest chance of donor regeneration and therefore unlimited donor.

One thing is that your say girls can pluck their eyelashes over and over again without damage but don't you believe there is a limit? We know with head hair that people can end up with bald spots from traction alopecia, so if you pluck the hairs too many times won't they stop growing?

[drnigams]

Kindly find below the microscopic images of Day 7 Donor and Day 9 recipient of 15 G patch test of in-vitro hair doubling.

We have extracted 15 grafts from the donor area and were bisected to make it to 30 grafts. 15 grafts were implanted back into the donor area and 15 grafts were implanted into the recipient area.

There were 8 Single grafts, 6 Double grafts & 1 Triple grafts, so 23 follicles were used in this procedure.

Visibility of 46 follicles means 100% regrowth anything less or more is accordingly.

Final results can be analysed after 6 months to see if some more hair regrow which may be in Telogen or in development stage.

GC, Didi & Ironman can do independent marking on the same. Because shortly the marks on the donor and recipient will disappear.

Donor microscopic images



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/1.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/2.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/3.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/4.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/5.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/6.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/7.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/8.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...er/Large/9.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/10.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/11.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/12.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/13.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/14.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...r/Large/15.jpg

Recipient microscopic images



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/1.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/2.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/3.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/4.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/5.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/6.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/7.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/8.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...nt/Large/9.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/10.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/11.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/12.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/13.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/14.jpg



click below link to see enlarged close up of the above pic
http://www.drnigams.net/images/RTN/D...t/Large/15.jpg

Dr nigam,

we need procedure that will overcome HSTs limitations, (very small sessions spaced at least 9 months apart), for high NWs is just not practical...I mean, who can wait 6+ years to finish process...plus prices which are sky high, taking advantage of unlucky sufferers

Iron man will be 60 years old by the time he receives 10 000 hst grafts..no wonder he exibits psychopathic/anti-social behavior

[drnigams]

We have sent a proposal to Blue Seed outside USA for a small stem cells lab and small clinic. They will get back to us regarding regulation. Blue Seed is dominated by silicon valley professionals and innovator.

Let me tell you forum members know more about stem cells and hair doubling than many non-western doctors. When I advertise Hair Doubling and Donor Doubling in newspaper forget about patient it is a alien language for even doctors.

You will be shocked stem cells regulatory body is not formed still in many country and in India it is in early phase and director are yet to be appointed. If I am little fortunate I will be in the advisory board of Indian Stem cells regulatory body. I have served as an Adviser to my State FDA regarding International regulatory advice on cosmoceuticals (which are neither classified as drug nor classified as over the counter non-prescription product)
My term as an FDA adviser expired five year ago because I cannot give so much time. But this stint as an FDA advisor helped me to understand pros & con of regulatory procedures and how one can work around it as a doctor with new therapeutic clinic based invention or procedures.


You're certainly researching a lot of avenues Dr. Nigam and that is both extremely refreshing and exciting. It says that Blueseed will hopefully launch between Q2 and Q3 next year so by the end of 2014 you could have your own clinic offering all your procedures in US waters, is that correct? Will you be seeking FDA and other regulatory approval whilst offering these treatments to those who want to travel out of regulatory jurisdiction?

Dr. Nigam how are you finding the time to do all this stuff!? and then come on and answer all our questions so promtly, maybe you're further along in cloning than we realise and you've cloned yourself

Given that regualtions are different in India why are there not other India doctors working on this? Or why haven't companies such as Aderans and Replicel done work in India?

Please continue to update us on all your new ventures and good luck with everything Dr. Nigam.[/QUOTE]

[Arashi]

Dr Nigams, I must admit, it's hard to not get excited when you read your postings. But please understand, we need to see solid results.

I really don't understand why NSN all of a sudden doesn't visit the forums anymore. Yes he had personal problems but that didn't keep him from posting. His case would be the first in hopefully a row of solid proof of your techniques. Please understand that this 15 graft test you're performing can't be considered as reliable proof because you're doing it on a staff member.

[drnigams]

Didi,

We do approximately 8 cases a day, all are stem cells hair multiplication + DP culture and atleast 1 a day. In-vivo donor doubling with stem cells.

As told to you we have stopped doing FUE unless we have to do free to those who did not get stem cells result. Because donor doubling takes lots of time and since for FUE we require 2 staffs and for Donor Doubling we require 6 staffs.

Didi my in-vivo hair doubling with stem cells + DP cell & DP injection require 2 sessions of 3 days each 4 to 6 months apart should be sufficient to convert NW7 to NW2. Hopefully we will be able to document the same in coming months.

Lets see what happen with plucked hair transplant with stem cells in coming 15 to 30 days.

Yesterday I did 2100 grafts donor doubling in-vivo to 4200 grafts. This patient is from other state of India. I will post his photos and also will update the photos of 5000 grafts doubling to 10000 graft on a HT doctor from Delhi post 1 month procedure. His other pictures are already posted on HS thread.

Dr nigam,

we need procedure that will overcome HSTs limitations, (very small sessions spaced at least 9 months apart), for high NWs is just not practical...I mean, who can wait 6+ years to finish process...plus prices which are sky high, taking advantage of unlucky sufferers

Iron man will be 60 years old by the time he receives 10 000 hst grafts..no wonder he exibits psychopathic/anti-social behavior

[drnigams]

GC knows the answer why NSN is not posting due to certain forum members specially IM. GC knows the good progress of the recipient of NSN case directly from NSN.

Let us leave him alone, after all he is now my patient. His privacy is important. He is no more only a forum member to me but also a patient. I am embarrassed because of silly mistakes of my staff. Although wrong identity of his but still Ironman did very bad to him by posting his wrong identity pictures on the forum. If the same will happen to you and me do you think we will still remain the forum? I sincerely apologize to NSN for the same.

Dr Nigams, I must admit, it's hard to not get excited when you read your postings. But please understand, we need to see solid results.

I really don't understand why NSN all of a sudden doesn't visit the forums anymore. Yes he had personal problems but that didn't keep him from posting. His case would be the first in hopefully a row of solid proof of your techniques. Please understand that this 15 graft test you're performing can't be considered as reliable proof because you're doing it on a staff member.

[Arashi]

GC knows the answer why NSN is not posting due to certain forum members specially IM. GC knows the good progress of the recipient of NSN case directly from NSN.

Let us leave him alone, after all he is now my patient. His privacy is important. He is no more only a forum member to me but also a patient. I am embarrassed because of silly mistakes of my staff. Although wrong identity of his but still Ironman did very bad to him by posting his wrong identity pictures on the forum. If the same will happen to you and me do you think we will still remain the forum? I sincerely apologize to NSN for the same.

??? I'm confused here. Iron Man posted name and address of NSN ?

[drnigams]

Caddarick79,
You must have noticed ,i am not selling or calling members myself,it is a big responsibility.
Things will be clear with documentation of independent senior forum members.I am not even charging them for patch test.
Still those who believe me as adoctor with gut feeling and little documentation are booking themselves.
I have 90plus enquires from forum members and viewers,i told them to come from july,except 5 to 6 who will be coming for large grafts in april and may.
I would love to recieve you in india after 2 months,with more documentations and improvements.

Dear Dr Nigam,

You seem promising and very confident with the future of hair multiplication>
At this moment, I still need some more proofs and that you become more mainstream>>> but for sure, if you become the reference and if all what you claim is becoming concrete, approved and real, i will not mind flying to India to get my hair restored at a reasonnable fare and reasonnable rythm>

Can you summarise a bit about where we are TODAY and what you are sure you will reah withing 2 or 3 years?

Thank you

[Arashi]

GC knows the answer why NSN is not posting due to certain forum members specially IM. GC knows the good progress of the recipient of NSN case directly from NSN.

Let us leave him alone, after all he is now my patient. His privacy is important. He is no more only a forum member to me but also a patient. I am embarrassed because of silly mistakes of my staff. Although wrong identity of his but still Ironman did very bad to him by posting his wrong identity pictures on the forum. If the same will happen to you and me do you think we will still remain the forum? I sincerely apologize to NSN for the same.

Seriously Dr Nigams, I don't know what to think of all this. If I understand your post correctly your staff members revealed photo's of NSN's face to IronMan who posted them ? If that's true I can imagine NSN is pissed at you. But still, he should also understand that there are tons of people out there interested in his results.

I just don't know what to think of this. I WANT to believe you but again, the skepticism in me just takes over. I find it very weird that by now we still haven't seen any pictures (except that mole, which is no proof at all without the rest of the donor).

[didi]

i think dr nigam posted pic where nsn name was written or smthing...IM made sure it spreads, its fake name anyway but IM likes to rub it in...