InVitro5000G HAIR DOUBLING to 10000G@DRNIGAMS

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  • drnigams
    Senior Member
    • Nov 2012
    • 551

    InVitro5000G HAIR DOUBLING to 10000G@DRNIGAMS

    Dear Friend's / Critics.Presenting a case of 5000 grafts doubling to 10000 grafts in 3 days on a male hair transplant doctor himself from delhi, 27years ,NW5/6 WITH THE AIM TO CONVERT TO NW1/2.aFTER THE EXTRACTION OF THE GRAFTS FROM THE DONOR SCALP,INVITRO BISECTION WAS DONE UNDERV MAGNIFICATION.aLL THE DONOR BISECTED GRAFTS WERE IMPLANTED AT THE RECIPIENT AND NONE AT THE DONOR AS PER PATIENTS INSTRUCTIONS AS HE WILL BE GETTING MARRIED EARLY NEXT YEAR.Also added to each bisected graft wasprogenitor stemcells,dp cells isolated from the chest hair.Also added were EXTRACELLULAR MATRIX,prp,and 6 growth factors to boost the survival of the grafts.This is as per my knowledge world's 1st documentation of invitro hair doubling with boosters.
    I personally believe this is much better technique than my own donor doubling in vivo which was done for neversay never as he did not want an fue extraction of grafts.Since this is not the blind technique.Will keeep you all updated with followup pics.
    Regards dr.nigam

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    Day 0, Day 11 & Day 12 front view of the recipient where all the 10000 bisected grafts were implanted.

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    Day 0 & Day 11 side view were 2500 grafts were doubled to 5000 grafts and all implanted at the recipient. On day 12 further 2500 grafts were doubled to 5000 grafts & implanted at the recipient. Day 12 same angle pic not available.

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    Day 0, Day 11 & Day 12 vertex with bisected grafts.

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    Day 0 & Day 11 side view were 2500 grafts were doubled to 5000 grafts and all implanted at the recipient. On day 12 further 2500 grafts were doubled to 5000 grafts &implanted at the recipient. Day 12 same angle pic not available.

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    Extracted grafts with .5mm inner diametre hollow needle.Kept in special preservation medium(this is a reference pic of nsn case.

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    Invitro bisection of grats just above the dermal papilla.AS YOU CAN SEE ALL THE FOLLICLES OF FOLLICULAR UNIT ARE BISECTED AND IMPLANTED.

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    BISECTED GRAFTS IN VITRO.

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    50X MAGNIFICATION MICROSCOPE WITH ANDROID WITH HAIR SOFTWARE USED FOR STUDY OF BISECTED GRAFTS.

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    aLL THE IMPLANTED GRAFTS WERE INJECTERD WITH PROGENITOR STEMCELLS,DP CELLS FROM CHEST FOLLICLES.aLSO ADDED WERE 6 GROWTH FACTORS ,PRP AND EXTRACELLULAR MATRIX AS BOOSTER TO THE GRAFTS FOR BETTER SURVIVAL.

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  • Kiwi
    Senior Member
    • Mar 2011
    • 1105

    #2
    Thanks for the update doc.

    Do you need an older member of this site to volunteer? I have blonde hair and I would be willing to shave my head and document the process if it included FUE into my old FUT scar.

    Comment

    • Kiwi
      Senior Member
      • Mar 2011
      • 1105

      #3
      Also looking forward to ironmans assessment. Healthy non patronizing and good mannered debate is welcome.

      Comment

      • drnigams
        Senior Member
        • Nov 2012
        • 551

        #4
        Kiwi,
        You can mail me your pics dr.rahul1970@gmail.com I may select you as a volunteer,once i look at your donor and recipient scalp,the density,your strip scar.It will be great if you have any birth mark or will you be ready for atiny dot tattoo for credible documentation.
        Originally posted by Kiwi
        Thanks for the update doc.

        Do you need an older member of this site to volunteer? I have blonde hair and I would be willing to shave my head and document the process if it included FUE into my old FUT scar.

        Comment

        • wesleybelgium
          Junior Member
          • Mar 2013
          • 16

          #5
          keep up the good work.... we need pioneers like you

          good work dr nigams

          im glad that there are doctors that dare to make a diffrence beyond regular fue ht's...

          i'm also a hardcore hairlossveteran i have a before and 2 after pics on my profile

          and your hairdoubling and hairmultiplication intrests me verry much
          sinds i have only 1000 to 2000 grafts max donor left afer 2 hairtransplants
          the one thing that intrests me the most is increasing density on top... i allready did 2x prp sessions also , with no results
          in my public info you can see my impressive list of hairmeds i'm also using , still i think i need growthfactors to get the results i want

          i can only say , keep up the good work and maybe we meet some day , i have this summer some vacation left , maybe for a trip to india , who knows

          Comment

          • One
            Senior Member
            • Oct 2012
            • 132

            #6
            News?

            Dr Nigam why do not update the evolution of the cases? So it becomes impossible to understand your technique. There are dozens of cases on your website still early days.

            Comment

            • Pentarou
              Senior Member
              • Apr 2013
              • 484

              #7
              I agree, Dr Nigam really needs to keep some of these cases updated.

              Dr Nigam also needs reliable professional photography for his cases too.

              Comment

              • drnigams
                Senior Member
                • Nov 2012
                • 551

                #8
                One,
                Spoke to this patient yesterday,except 15% shedding he is observing good growth all over the implanted area.
                Regarding his photos ,will post when he comes to see me at mumbai clinic, in mid may 2013.
                In the meantime he has posted few pics which i have uploaded at HS,
                and in one week he has promised to send me some more pics...but when the patient pics are not of same angle,lighting....i prefer to take pics at my clinic and then post on the forums.
                Kindly have a look at nw7 to nw2 thread tonight ,the patient have sent me his pics ..which are encouraging

                QUOTE=One;119386]News?

                Dr Nigam why do not update the evolution of the cases? So it becomes impossible to understand your technique. There are dozens of cases on your website still early days.[/QUOTE]

                Comment

                • 534623
                  Senior Member
                  • Oct 2011
                  • 1865

                  #9
                  Originally posted by drnigams

                  Dear Critics.
                  Hi. How is the weather on your planet?

                  Originally posted by drnigams
                  Presenting a case of 5000 grafts doubling to 10000 grafts in 3 days on a male hair transplant doctor himself from delhi, 27years ,NW5/6 WITH THE AIM TO CONVERT TO NW1/2.

                  - aFTER THE EXTRACTION OF THE [5000] GRAFTS FROM THE DONOR SCALP,
                  - INVITRO BISECTION WAS DONE UNDERV MAGNIFICATION.aLL THE DONOR BISECTED GRAFTS WERE IMPLANTED AT THE RECIPIENT AND NONE AT THE DONOR
                  So, you extracted (via normal FUE?) 5000 grafts/FU's from the patient's donor scalp, then you bisected these 5000 extracted grafts outside the body under magnification to get 10,000 bisected grafts for implantation into the recipient area.

                  Does it mean, and if I count correctly, that after 3 days this patient ended finally up with (all in all) 15,000 (!) holes and/or slits in his head?

                  How did you get the 5,000 FUE grafts from this patient's donor area??
                  I mean, in all your posted photos, including the after implantation photos - it seems you didn't shave the whole head. Actually, I can't even see the back of the patients head (donor area) shaved in the pics. I mean, for 5000 (!) FUE extractions, you need either a very large area to get 5000 grafts or you must extract the grafts very close to each other - OR you simply performed a "golden standard" procedure; namely, a normal STRIP/FUT procedure in the donor area. But in your graft-photos, I can't see typical FUT grafts. The grafts rather look like typical FUE grafts.

                  So HOW did you get the 5,000 grafts from this patient's donor area, without shaving the whole donor area??

                  Originally posted by drnigams
                  Also added to each bisected graft wasprogenitor stemcells,dp cells isolated from the chest hair.Also added were EXTRACELLULAR MATRIX,prp,and 6 growth factors to boost the survival of the grafts.
                  Progenitor stem cells?
                  You say you got (of course within just 3 days) sufficient DP cells from the patient's chest hair. But HOW and FROM WHERE did you get "Progenitor stem cells"?

                  Have you EVER tested what such a mix of the following components

                  - DP cells
                  - pregenitor stem cells
                  - Extracellular matrix
                  - PRP platelet rich plasma
                  - 6 different growth factors

                  ... can cause in the body?
                  And how do you know that the combination of all these things have to ability "to boost the survival of the grafts"??

                  Originally posted by drnigams
                  [ATTACH]20065[/ATTACH]
                  Invitro bisection of grats just above the dermal papilla.AS YOU CAN SEE ALL THE FOLLICLES OF FOLLICULAR UNIT ARE BISECTED AND IMPLANTED.

                  [ATTACH]20064[/ATTACH]

                  [ATTACH]20063[/ATTACH]
                  BISECTED GRAFTS IN VITRO.
                  Sorry, but NONE of these photos show a bisection of grafts "just above the dermal papilla".
                  For example, this photo ...



                  ... shows bisected grafts, whereas the distal parts show multiple hairs, but not the proximal parts below them - and NONE of the bisected grafts shows a bisection "just above the dermal papilla". That indicates, that you still have no clue where the DP is located in a hair follicle.

                  Originally posted by drnigams
                  AS YOU CAN SEE ALL THE FOLLICLES OF FOLLICULAR UNIT ARE BISECTED AND IMPLANTED.
                  Sorry, but that is something I can't see in any of your photos.

                  Comment

                  • Dees Dab
                    Member
                    • Apr 2013
                    • 43

                    #10
                    Dr Nigam, it took three days? Can you describe in a little more detail what u did in the first, second and third day of your procedure?

                    And why no pre and post op pics of the donor whether head and/or body?

                    Also will there be white dot scarring at the donor visible if the patient cuts his hair very short?

                    Comment

                    • drnigams
                      Senior Member
                      • Nov 2012
                      • 551

                      #11
                      Welcome Ironman,
                      Now that you have arrived..i hope thunderstorms are predicted...!
                      1)Yes 5000G extracted with .6mm/.7mm/.8mm punches as per single ,double and triple follicle graft.Yes fine incisions at the recipient approx.10000.
                      2)Safe donor area of this patient was approx.300sqcms,and his density at the donor was approx.50G per sqcm.Which means he has a safe donor area with 15000grafts,of which we have extracted 5000 grafts and implanted all the bisected grafts at the recipient as per the patients wish.
                      3)We have shaved more,will post the pics in mid may when the patient arrives from delhi.What you are seeing is shaving on day 0.
                      4)Few grafts from the donor scalp and chest were sent to the lab for isolation activation(progenitor) of stemcells (bulge,outer root sheath,dermal cup sheath,dermal mesenchymal stemcells).It takes approx. 4 hours for recieving the progenitor stemcells from the lab from the extracted follicles.Multiplied stemcells will be given to the patient when he comes to clinic in mid may,it takes minimum 6weeks for 4 to 5 passages to have atleast 1million stemcells.
                      5)Regarding survival of grafts and effect of these cells and factors,i will cover in next post.
                      Down with little fever and lot of pending work for edinburgh conference.
                      60 i have purposely not shown the right bisection ,will post the same tmr.The idea is to bisect at the level of dermal cup sheath around aubers line ,so that both the parts have mesenchymal cells.

                      Bottom line is ..i want certain part containing mesenchymal dermal cells of the root of the follicle.
                      Originally posted by 534623
                      Hi. How is the weather on your planet?


                      So, you extracted (via normal FUE?) 5000 grafts/FU's from the patient's donor scalp, then you bisected these 5000 extracted grafts outside the body under magnification to get 10,000 bisected grafts for implantation into the recipient area.

                      Does it mean, and if I count correctly, that after 3 days this patient ended finally up with (all in all) 15,000 (!) holes and/or slits in his head?

                      How did you get the 5,000 FUE grafts from this patient's donor area??
                      I mean, in all your posted photos, including the after implantation photos - it seems you didn't shave the whole head. Actually, I can't even see the back of the patients head (donor area) shaved in the pics. I mean, for 5000 (!) FUE extractions, you need either a very large area to get 5000 grafts or you must extract the grafts very close to each other - OR you simply performed a "golden standard" procedure; namely, a normal STRIP/FUT procedure in the donor area. But in your graft-photos, I can't see typical FUT grafts. The grafts rather look like typical FUE grafts.

                      So HOW did you get the 5,000 grafts from this patient's donor area, without shaving the whole donor area??


                      Progenitor stem cells?
                      You say you got (of course within just 3 days) sufficient DP cells from the patient's chest hair. But HOW and FROM WHERE did you get "Progenitor stem cells"?

                      Have you EVER tested what such a mix of the following components

                      - DP cells
                      - pregenitor stem cells
                      - Extracellular matrix
                      - PRP platelet rich plasma
                      - 6 different growth factors

                      ... can cause in the body?
                      And how do you know that the combination of all these things have to ability "to boost the survival of the grafts"??


                      Sorry, but NONE of these photos show a bisection of grafts "just above the dermal papilla".
                      For example, this photo ...



                      ... shows bisected grafts, whereas the distal parts show multiple hairs, but not the proximal parts below them - and NONE of the bisected grafts shows a bisection "just above the dermal papilla". That indicates, that you still have no clue where the DP is located in a hair follicle.


                      Sorry, but that is something I can't see in any of your photos.

                      Comment

                      • drnigams
                        Senior Member
                        • Nov 2012
                        • 551

                        #12
                        Grafts which you have mentioned in your post are of invivo bisection ..
                        Invitro technique gives us option of better precision for bisection and in invivo the bisection points will vary technician to technician and graft to graft..but the intention is to have atleast part of outer root sheath and or part of mesenchymal cells in case of invivo technique.
                        And invitro technique the intention is to have atleast a part of mesenchymal cells or dermal cup sheath cells.
                        Originally posted by drnigams
                        Welcome Ironman,
                        Now that you have arrived..i hope thunderstorms are predicted...!
                        1)Yes 5000G extracted with .6mm/.7mm/.8mm punches as per single ,double and triple follicle graft.Yes fine incisions at the recipient approx.10000.
                        2)Safe donor area of this patient was approx.300sqcms,and his density at the donor was approx.50G per sqcm.Which means he has a safe donor area with 15000grafts,of which we have extracted 5000 grafts and implanted all the bisected grafts at the recipient as per the patients wish.
                        3)We have shaved more,will post the pics in mid may when the patient arrives from delhi.What you are seeing is shaving on day 0.
                        4)Few grafts from the donor scalp and chest were sent to the lab for isolation activation(progenitor) of stemcells (bulge,outer root sheath,dermal cup sheath,dermal mesenchymal stemcells).It takes approx. 4 hours for recieving the progenitor stemcells from the lab from the extracted follicles.Multiplied stemcells will be given to the patient when he comes to clinic in mid may,it takes minimum 6weeks for 4 to 5 passages to have atleast 1million stemcells.
                        5)Regarding survival of grafts and effect of these cells and factors,i will cover in next post.
                        Down with little fever and lot of pending work for edinburgh conference.
                        60 i have purposely not shown the right bisection ,will post the same tmr.The idea is to bisect at the level of dermal cup sheath around aubers line ,so that both the parts have mesenchymal cells.

                        Bottom line is ..i want certain part containing mesenchymal dermal cells of the root of the follicle.

                        Comment

                        • One
                          Senior Member
                          • Oct 2012
                          • 132

                          #13
                          Dr Nigam and Dr. Poswal because do you have the same patient with the same photo?


                          http://drnigamsthane.com/gallery/ Nigam

                          http://bellicapelli.forumfree.it/?t=61747722 Poswal

                          Comment

                          • Arashi
                            Senior Member
                            • Aug 2012
                            • 3888

                            #14
                            Originally posted by One
                            Dr Nigam and Dr. Poswal because do you have the same patient with the same photo?


                            http://drnigamsthane.com/gallery/ Nigam

                            http://bellicapelli.forumfree.it/?t=61747722 Poswal
                            Oh my god !!! I'm dying of laughter here What a joke. Let's see how Dr Nigams is going to explain this one Nah he'll just blame his new webdeveloper (he's probably just as stupid as the old one who photoshopped patient photo's, haha). What a joke !

                            Comment

                            • hellouser
                              Senior Member
                              • May 2012
                              • 4423

                              #15
                              Originally posted by One
                              Dr Nigam and Dr. Poswal because do you have the same patient with the same photo?


                              http://drnigamsthane.com/gallery/ Nigam

                              http://bellicapelli.forumfree.it/?t=61747722 Poswal

                              Comment

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