Tsuji labs news????

[fadingaway]

So your 4,000+, anti-scientific, negative crusading posts have just been sarcasm? Awesome.

I think someone doesn't understand the difference between sarcasm and pessimism.

[Renee]

Anybody aware of this patent filed by tsuji in February 2014? It's about controlling the color of the implanted hair follicle using his method from 2012.

FOLLICLE GERM FOR TRANSPLANTATION IN WHICH HAIR COLOR IS CONTROLLED, COMPOSITION INCLUDING REGENERATED HAIR FOLLICLE GERM FOR TRANSPLANTATION, AND METHOD OF TRANSPLANTING REGENERATED HAIR FOLLICLE GERM

http://www.faqs.org/patents/app/20140052167

[lacazette]

Sounds good renee, it could confirm his claims that he succesfully grown ectodermal organs for the moment ( teeth, hair follicles, and some secretory glands) with his organ germ method.

His lab is part of the RIKEN CENTER FOR DEVELOPMENTAL BIOLOGY ( there's 20 different labs)
http://www.cdb.riken.jp/en/research/...rog_index.html

there's other lab that are interesting. One lab work on iPSC, one on tissue, one on growth control signaling, 2 others work on cells adhesion, cells asymetry,etc..


Here's the link of tsuji lab, called "riken laboratory for organ regeneration"
http://www.cdb.riken.jp/org/en/topics/index.html

[lacazette]

Another lab of tsuji called "Four-Dimensional Multicellular Dynamics Laboratory"
http://www.riken.jp/en/research/labs...multicell_dyn/

•Development of automatically cell analysis system for 4-dimensional multicellular dynamics in organogenesis

[lacazette]

In their last publish study Jul2015

"This bioengineered organ germ method, which manipulates epithelial and mesenchymal cells via cell compartmentalization at a high cell density in a type I collagen gel matrix, was developed to reconstruct bioengineered organ germs in vitro as an organ engineering technology (Figure 2a,b) [91,92]. This method successfully developed bioengineered ectodermal organs, such as teeth and hair follicle germs, through multicellular assembly and epithelial and mesenchymal interactions similar to those in natural organ germs (Figure 2c,d) [91,92,93,94,95]. Importantly, the bioengineered tooth and hair follicle germ transplants could restore physiological functions via cooperation with peripheral tissues at the lost tooth or hair follicle "

"These studies are a proof-of-concept for bioengineered organs that can functionally restore the lacrimal gland"


In 2012:

"Recently, Toyoshima and colleagues (Tsuji Lab) created a bioengineered hair follicle germ using embryonic skin-derived epithelium and mesenchymal donor cells. The transplanted germ integrated with the host epithelium, producing a fully functional hair follicle demonstrating connection to the host nerves as well as to the piloerection ability. They further demonstrated that through the use of a guide, the interaction between these donor cells and the host epithelium was independent of placement depth. Taken together, these studies demonstrate that cells delivered in the appropriate package are able to integrate with the host tissue to produce a hair follicle. This technique would provide the clinician the ability to control the pattern, density, and orientation of the follicles resulting from this cell-based therapy."


That was before iPSC breaktrought, so now in 2015, if they can do the same with iPSC rather than embryonic, it's sounds like the cure, but are they trying it on human trial? Or do they need more time on mouse to perfect the protocol? maybe it's more the second option (even if I really hope Im wrong)

In 2012 they were saying they plan to begin human trials within 3-5 years. So let's cross fingers trials begin soon, with regenerative law in japan, it could hit the market there before 2020

[lacazette]

(Tsuji Lab) created a bioengineered hair follicle germ using embryonic skin-derived epithelium and mesenchymal donor cells.

The two are now possible with iPSC

xu team : "The researchers then converted iPSCs into a large number of epithelial stem cells" 2014

And for mesenchymal also :

"Here we report generation of induced pluripotent stem cells (iPSC)-derived MSCs.
Starting from iPSCs with almost unlimited expandability, the protocol can be readily scaled up to provide huge amounts of MSCs with uniform biological properties for multicenter evaluation, large animal experiments, and potential clinical trial"
2014

Come on tsuji test it on human!!

[Renee]

On the university website dr Tsuji says hair will be available in 5-10 years and teeth regeneration will be available in 2-3 decades.

[Renee]

Lacazette, so he will grow the follicles in a dish and transplant it one by one? Or it will be an injection?

[lacazette]

Hey Renee, seems taht the text with this quote is from 2013 (bottom of the page)

(If we don't take in consideration that he could have unespected issues, or at contrary the progress of technologies and knowledge those last two years), we could probably talk more about 3-8 years for the public. Let's hope exponential progress will diminish the timeline if they're not already in human trial

@Renee, on their mouse study they grew 'HF germ' and they injected them. But I also see they use the word 'transplanted them'
So maybe in this organ germ method, 'accurate injection of a hair germ' and 'transplantation of a hair germ' means the same. Or maybe there really are two ways to implant them i don't know.

Quote from Desmond in a 2013 topic :

According to their last study, they injected 24 "hair germs" into a 1cm2 area to yield approximately 120 hairs/cm2.

[Swooping]

I have recently spoken to Dr. Takashi Tsuji. They are still encountering problems, most notably the critical issue of expansion of the epithelial bulge stem cells. When they overcome the problems they will initiate a pre-clinical study to test for safety followed by human clinical trials. He is confident that the method will succeed in humans too.

It's a shame I can't speak Japanese to get to know more from this very bright yet humble person. They are probably the closest too a full cure imo. We'll have to wait, definitely worth following though. Might be our hero one day .

Real research, real possibilities.