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I share here, big thanks to NeedHairAsap for this awesome find,
Seems that these plastic surgeons have built the protocol to apply after wounding that Follica is searching
October 9, 2015 - American Society of Plastic Surgeons
Transplantation of a LGR6+ Epithelial Stem Cell-Enriched Scaffold for Repair of Full-Thickness Soft Tissue Defects: The In-Vitro Development of Polarized Hair Bearing Skin.
Lough, Denver MD,PhD; Wetter, Nathan MD; Madsen, Christopher MD; Reichensperger, Joel BS; Cosenza, Nicole MS; Cox, Lisa BS; Harrison, Carrie BS; Neumeister, Michael MD
Background: The LGR6 expressing epithelial stem cell (LGR6+ ESC), within the follicular bulge, is capable of producing all cellular lineages and concomitant structures of the skin and interfollicular epidermis, including keratinocytes, sebaceous gland and hair follicle. Literature has shown that full thickness wounds, devoid of the stem cell niche, can subsequently be reconstructed with functional skin elements following migration of the LGR6+ ESC into the wound bed. Here, we employ a variety of LGR6+ ESC-seeded scaffolds to determine therapeutic potential in immediate reconstruction of full-thickness wounds with polarized functional hair-bearing skin.
Methods: Isolated LGR6+GFP ESCs were seeded on a spectrum of acellular matrices and monitored in both in vitro and in vivo environments. These studies were used to determine the ability of the LGR6+GFP ESC population to maintain viability, proliferation and migration processes as well as wound healing and nascent hair follicle development.
Results: Wound beds containing LGR6+GFP ESCs seeded scaffolding showed significantly augmented rates of healing, epithelialization and hair growth when compared to controls. Gene and proteomic expression studies indicate that LGR6+GFP ESC seeded matrices up-regulate wingless-type MMTV integration site family (WNT), epidermal growth factor (EGF) and angiogenesis pathways. Finally, the addition of ADSc containing SVF to LGR6+GFP ESC-seeded scaffolding, induces polarity cellular populations, while further augmenting pro-angiogenic analytes.
Conclusions: LGR6+GFP ESCs are able to undergo proliferation, differentiation and migration on simple collagen-based scaffolding while augmenting in vivo epithelialization, hair growth and angiogenesis within wound beds. The addition of SVF to LGR6+GFP ESC-seeded scaffolding induces an early form of tissue polarization, providing for the first time, a clinically applicable stem cell based construct for the repair of full thickness wounds and hair regeneration.
Seems that these plastic surgeons have built the protocol to apply after wounding that Follica is searching
October 9, 2015 - American Society of Plastic Surgeons
Transplantation of a LGR6+ Epithelial Stem Cell-Enriched Scaffold for Repair of Full-Thickness Soft Tissue Defects: The In-Vitro Development of Polarized Hair Bearing Skin.
Lough, Denver MD,PhD; Wetter, Nathan MD; Madsen, Christopher MD; Reichensperger, Joel BS; Cosenza, Nicole MS; Cox, Lisa BS; Harrison, Carrie BS; Neumeister, Michael MD
Background: The LGR6 expressing epithelial stem cell (LGR6+ ESC), within the follicular bulge, is capable of producing all cellular lineages and concomitant structures of the skin and interfollicular epidermis, including keratinocytes, sebaceous gland and hair follicle. Literature has shown that full thickness wounds, devoid of the stem cell niche, can subsequently be reconstructed with functional skin elements following migration of the LGR6+ ESC into the wound bed. Here, we employ a variety of LGR6+ ESC-seeded scaffolds to determine therapeutic potential in immediate reconstruction of full-thickness wounds with polarized functional hair-bearing skin.
Methods: Isolated LGR6+GFP ESCs were seeded on a spectrum of acellular matrices and monitored in both in vitro and in vivo environments. These studies were used to determine the ability of the LGR6+GFP ESC population to maintain viability, proliferation and migration processes as well as wound healing and nascent hair follicle development.
Results: Wound beds containing LGR6+GFP ESCs seeded scaffolding showed significantly augmented rates of healing, epithelialization and hair growth when compared to controls. Gene and proteomic expression studies indicate that LGR6+GFP ESC seeded matrices up-regulate wingless-type MMTV integration site family (WNT), epidermal growth factor (EGF) and angiogenesis pathways. Finally, the addition of ADSc containing SVF to LGR6+GFP ESC-seeded scaffolding, induces polarity cellular populations, while further augmenting pro-angiogenic analytes.
Conclusions: LGR6+GFP ESCs are able to undergo proliferation, differentiation and migration on simple collagen-based scaffolding while augmenting in vivo epithelialization, hair growth and angiogenesis within wound beds. The addition of SVF to LGR6+GFP ESC-seeded scaffolding induces an early form of tissue polarization, providing for the first time, a clinically applicable stem cell based construct for the repair of full thickness wounds and hair regeneration.
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