Anyone have any news from Lauster/Jahoda teams?

[kuba197]

http://www.hcplive.com/medical-news/major-breakthrough-in-skin-cell-growth

Takashi Tsuji also used a Wnt-10b molecule.

[allTheGoodNamesAreTaken]

One scalp hair follicle has ~3000 DPC.

OK so for a, let's say, 5000 graft transplant, you would need 3000*5000 = 15 million DP cells... and if you want to get all of that by starting from one cell, you'd need 24 passes... or of you start with 100 cells, then 17 passes gets you 13,107,200 and 18 passes gets you 26,214,400. Or if you start with 1000 cells that's 14 passes required to hit 15,000,000 total cells.

Is that an absurd number of passes to hope is achievable?

In fact, if you want that many DP cells from the currently achievable 8 passes, you need to start with about 60,000 cells, which is only 20 follicles' worth. Why isn't this already doable?

[Swooping]

Latest patent Berlin team by the way; http://patents.justia.com/patent/20140370070.

It is particularly the expansion of the isolated DPFs and the physiological administration form that represent limiting factors to the use of these cells in hair growth induction of prior art. It takes several multiplication cycles to achieve the required quantity of cells, during which process the cells cultured in monolayer cultures, especially those of the dermal papilla fibroblasts, lose their inductive abilities, namely, after 5-8 multiplication cycles as shown by experience. The cells thus treated dedifferentiate and express stem cell markers, but can no longer be used to generate hair follicles.

It has been surprisingly demonstrated by the inventors that the cells reach the level of differentiation and stabilization or regain their hair growth-inducing ability after several days to several weeks under the specific culture conditions of the invention, wherein the DPFs expanded following cell culturing are transferred at well-defined concentration into special non-adhesive cell culture vessels. In addition to the regain of inductive properties, it has been surprisingly found that as a result of active cell-cell contacts and exchange of signaling molecules, the cells subsequently form cell aggregates and differentiate. Under these specific conditions, the cells thus treated condense into approximately the shape and size corresponding to the physiological shape of a dermal papilla in a hair follicle following isolation. To this effect, the ratio of cells used/culture vessel surface is of crucial importance.

What can be deduced from table 5 is that with prolonged culture time, the expression level of genes involved in three dimensional arrangement and tissue formation is approaching the expression level observed in native dermal papilla fibroblasts.

[joachim]

it's relatively easy with the passages.
one passage means, the cells divided and duplicated themselves. so no matter of many cells a hair follicle consists, one passage just means (considering no loss in efficiency) that you make 2 hairs out of 1.
2 passages mean, you make 4 hairs out of 1. so every passage doubles the amount of cells (or hairs).

8 passages means you made 256 hairs out of 1 extracted donor hair.
10 passages mean you can make 1024 hairs.

the more passages can be achieved, the less hairs you have to extract from your donor region.
replicel also mentioned they need around 100 hairs or so to extract from the donor.
the better and the more efficient your culturing is, the less original donor hairs you need.

all in all, no matter if 8 or 10 successful passages were achieved. the gene expression and the resulting hair is the important thing here. achieving even more passages is just a nice bonus for making the exctraction at the donor site less invasive and faster.

[Swooping]

How many DP cells does one fully grown follicle have? By my calculations you have the following number of cells after this many passes:

Passes Cells
8 - 256
...
17 - 131,072
18 - 262,144
19 - 524,288
20 - 1,048,576
21 - 2,097,152
22 - 4,194,304
23 - 8,388,608
24 - 16,777,216
25 - 33,554,432

One scalp hair follicle has ~3000 DPC.

[joachim]

According to the latest study published on April 1st, WNT-10b maintains DP gene expressions for hair induction for up to 10 passages!!! That is better than the Chinese that was reported several weeks ago. This is what Aaron Gardiner was working on but gave up and switched to cancer research.

Guys, the Japs are all over this!!! It's amazing Here's the link to the paper:

http://link.springer.com/protocol/10.1007/7651_2016_319

Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro
BY Yukiteru Ouji, Masahide Yoshikawa

Let's hope the Japanese speed ahead with this discovery and blow people's expectations right out of the park

sounds great indeed, but are you sure the DP cells have fully maintained gene expression, or is it just about inductivity again? are the cultured cells fully equivalent to their origin?
do you have access to the full paper and are you going to read the whole thing?
please tell us that this time it's a real and a useful breakthrough with the DP cell culturing.

[Arashi]

This maintains DP Gene Expression, and I don't think the team mentioned before was able to do that.

They previously retained about 65% gene expression if I remember correctly. That was enough to produce hair but not enough to produce cosmetically viable hair. If they retained 100% gene expression, so a 100% copy of the original, then that would mean hairloss is cured. It would be all over the news, so I highly highly doubt that's what's happened here

[iaskdumbquestions]

I kinda agree. I welcome any kind of progress but just creating more hair follicles that arent cosmetically viable ... Not sure how this is good news. Or am I missing something ?

This maintains DP Gene Expression, and I don't think the team mentioned before was able to do that.

[allTheGoodNamesAreTaken]

How many DP cells does one fully grown follicle have? By my calculations you have the following number of cells after this many passes:

Passes Cells
8 - 256
...
17 - 131,072
18 - 262,144
19 - 524,288
20 - 1,048,576
21 - 2,097,152
22 - 4,194,304
23 - 8,388,608
24 - 16,777,216
25 - 33,554,432

[FooFighter]

Maybe someone who is good in english can email them?

[Arashi]

8, 10, 12, 20 passages who cares? 8 was enough I thought. So this just creates more hair follicles that suck, right? I don't understand what's so much more significant with that..

I kinda agree. I welcome any kind of progress but just creating more hair follicles that arent cosmetically viable ... Not sure how this is good news. Or am I missing something ?

[iaskdumbquestions]

I don't believe they were able to retain gene expression in the last study.

Yeah, you're right. I should probably start reading things instead of skimming

[NeedHairASAP]

According to the latest study published on April 1st, WNT-10b maintains DP gene expressions for hair induction for up to 10 passages!!! That is better than the Chinese that was reported several weeks ago. This is what Aaron Gardiner was working on but gave up and switched to cancer research.

Guys, the Japs are all over this!!! It's amazing Here's the link to the paper:

http://link.springer.com/protocol/10.1007/7651_2016_319

Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro
BY Yukiteru Ouji, Masahide Yoshikawa

Let's hope the Japanese speed ahead with this discovery and blow people's expectations right out of the park

This is why it's not that crazy that Thorn may just be betting on having the facility/staff etc. ready to piggy back on everyone else's discoveries.

[Pray The Bald Away]

I don't believe they were able to retain gene expression in the last study.

[iaskdumbquestions]

According to the latest study published on April 1st, WNT-10b maintains DP gene expressions for hair induction for up to 10 passages!!! That is better than the Chinese that was reported several weeks ago. This is what Aaron Gardiner was working on but gave up and switched to cancer research.

Guys, the Japs are all over this!!! It's amazing Here's the link to the paper:

http://link.springer.com/protocol/10.1007/7651_2016_319

Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro
BY Yukiteru Ouji, Masahide Yoshikawa

Let's hope the Japanese speed ahead with this discovery and blow people's expectations right out of the park

8, 10, 12, 20 passages who cares? 8 was enough I thought. So this just creates more hair follicles that suck, right? I don't understand what's so much more significant with that..